Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,652,131 G→T 9.2% P53T (CCG→ACG)  yfhM ← alpha2‑macroglobulin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,652,1310GT 9.2% 314.7 / 14.5 97P53T (CCG→ACG) yfhMalpha2‑macroglobulin
Reads supporting (aligned to +/- strand):  ref base G (46/42);  new base T (6/3);  total (52/45)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGT  >  NC_000913/2651983‑2652276
                                                                                                                                                    |                                                                                                                                                 
tGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGc                                                                                                                                                  >  2:1506541/1‑150 (MQ=255)
  aaTAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCAc                                                                                                                                                <  2:229719/150‑1 (MQ=255)
    tAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACgg                                                                                                                                              >  2:281802/1‑150 (MQ=255)
       cgcgTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGgaga                                                                                                                                           >  2:857763/1‑150 (MQ=255)
          gtgtGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGTGCCGGagagac                                                                                                                                        <  1:1447495/150‑3 (MQ=255)
           tGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGTGCCGGagagaca                                                                                                                                       <  1:331645/150‑4 (MQ=255)
              gaAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCGATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGc                                                                                                                                    >  2:804592/1‑150 (MQ=255)
              gaAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGc                                                                                                                                    >  2:606935/1‑150 (MQ=255)
                aaTCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTg                                                                                                                                  <  2:908145/150‑1 (MQ=255)
                  tCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGaa                                                                                                                                >  1:1397462/1‑150 (MQ=255)
                   ccTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAAc                                                                                                                               <  2:1127787/150‑1 (MQ=255)
                         ccGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGt                                                                                                                         >  1:1154656/1‑150 (MQ=255)
                            ggTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGtttt                                                                                                                      <  1:1280671/150‑1 (MQ=255)
                               cGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTg                                                                                                                   >  1:1261067/1‑150 (MQ=255)
                                 agagGGATGGAGAACGTCAGCACCAGCGTGGCGGCACCATCAAGTGGGACTTCAGAGACATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGCAGAGTTTTGCTGAACTCGCGCTTTCTGaa                                                                                                                 <  2:1284506/150‑1 (MQ=255)
                                  gagGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAag                                                                                                                >  2:1389983/1‑150 (MQ=255)
                                   agGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAaga                                                                                                               <  1:606935/150‑1 (MQ=255)
                                    gggATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAagag                                                                                                              >  1:1301751/1‑150 (MQ=255)
                                     ggATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGTGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAgg                                                                                                             >  2:268828/1‑150 (MQ=255)
                                     ggATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTAAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAgg                                                                                                             >  1:1317353/1‑150 (MQ=255)
                                      gATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGc                                                                                                            >  1:1157101/1‑150 (MQ=255)
                                      gATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGc                                                                                                            >  1:955224/1‑150 (MQ=255)
                                        tGGATAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCg                                                                                                          >  1:627927/1‑150 (MQ=255)
                                          gagaACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCt                                                                                                        <  1:1328111/150‑1 (MQ=255)
                                          gagaACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCt                                                                                                        >  2:1016587/1‑150 (MQ=255)
                                           agaACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCtt                                                                                                       >  1:379115/1‑150 (MQ=255)
                                           agaACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCtt                                                                                                       >  1:810024/1‑150 (MQ=255)
                                            taaCGTCATCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCttt                                                                                                      <  2:379845/149‑1 (MQ=255)
                                               cGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGt                                                                                                   <  2:1350693/150‑1 (MQ=255)
                                                gTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTa                                                                                                  >  1:1276449/1‑150 (MQ=255)
                                                gTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTa                                                                                                  >  2:106059/1‑150 (MQ=255)
                                                 tCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTaa                                                                                                 >  1:662670/1‑150 (MQ=255)
                                                    gCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACtt                                                                                              >  2:112204/1‑150 (MQ=255)
                                                     cACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTCCCGGCACTCTGGTGGTCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGTACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTc                                                                                             <  1:508812/150‑1 (MQ=255)
                                                      aCCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCa                                                                                            <  2:934022/150‑1 (MQ=255)
                                                       ccAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAg                                                                                           >  1:493151/1‑150 (MQ=255)
                                                       ccAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAg                                                                                           >  1:670472/1‑150 (MQ=255)
                                                          gCGTGGCTGCACCATCAAGTTTGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAg                                                                                        <  2:1216273/150‑1 (MQ=255)
                                                          gCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAg                                                                                        >  1:831886/1‑150 (MQ=255)
                                                          gCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAg                                                                                        <  2:1545486/150‑1 (MQ=255)
                                                          gCGTGGCTGCAACATCAAGTTGGACTTCAGAGAGATCCAGCAATTTAATAAAATTAGCTGCACTCTGTTTGGAGATTTTATGTCTCTACGTCACTTAGAGTTTTGCTGTAATATTGTTTTCTTAATAGTCCGATTTAGTAACTTaagaag                                                                                        <  2:1205255/150‑1 (MQ=255)
                                                           cGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAgg                                                                                       >  2:385166/1‑150 (MQ=255)
                                                             tGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGgcg                                                                                     <  1:1015335/150‑1 (MQ=255)
                                                              ggCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGgcgc                                                                                    <  1:1016587/150‑1 (MQ=255)
                                                                  gCACCATCAAGTTGGACTTCAGAGAGTTCCAGGAATTTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCTCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCt                                                                                <  2:1110576/150‑1 (MQ=255)
                                                                   cACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCCTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCtt                                                                               >  2:1623780/1‑150 (MQ=255)
                                                                       aTCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCtttttt                                                                           <  2:21660/150‑1 (MQ=255)
                                                                        tCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTc                                                                          <  1:857763/150‑1 (MQ=255)
                                                                             ttGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGc                                                                     <  1:1503474/150‑1 (MQ=255)
                                                                              tGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCt                                                                    >  2:1466100/1‑150 (MQ=255)
                                                                                   ttCAGAGAGATCCAGCGATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTgg                                                               <  1:804592/150‑1 (MQ=255)
                                                                                    tCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGgc                                                              <  2:379115/150‑1 (MQ=255)
                                                                                     cAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGGCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGgcg                                                             >  2:327524/1‑150 (MQ=255)
                                                                                     cAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGgcg                                                             >  2:114779/1‑150 (MQ=255)
                                                                                       gagagaTCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGcgcg                                                           >  2:991642/1‑150 (MQ=255)
                                                                                       gagagaTCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGcgcg                                                           >  1:774335/1‑150 (MQ=255)
                                                                                        agagaTCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGt                                                          <  2:1175424/150‑1 (MQ=255)
                                                                                           gaTCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTAt                                                       >  1:964179/1‑150 (MQ=255)
                                                                                             tCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCg                                                     <  1:340188/150‑1 (MQ=255)
                                                                                              ccAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCTTCTTTTTTCACCGCTGTTGGCGCGTTATCgt                                                    <  1:733645/150‑1 (MQ=255)
                                                                                               cAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCgtt                                                   <  2:604868/150‑1 (MQ=255)
                                                                                               cAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCgtt                                                   >  1:270995/1‑150 (MQ=255)
                                                                                                aGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCgttg                                                  <  1:1083165/150‑1 (MQ=255)
                                                                                                aGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCgttg                                                  >  2:1376272/1‑150 (MQ=255)
                                                                                                 gCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCgttgt                                                 >  1:1261847/1‑150 (MQ=255)
                                                                                                 gCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCgttgt                                                 <  1:1506541/150‑1 (MQ=255)
                                                                                                       tCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGc                                           <  1:281802/150‑1 (MQ=255)
                                                                                                       tCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGc                                           <  1:1244223/150‑1 (MQ=255)
                                                                                                        cAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCa                                          >  1:290261/1‑150 (MQ=255)
                                                                                                         aGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCAc                                         >  2:884196/1‑150 (MQ=255)
                                                                                                         aGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCAc                                         >  2:86437/1‑150 (MQ=255)
                                                                                                              cTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCACATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTgc                                    >  1:914009/1‑150 (MQ=255)
                                                                                                                     gCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTgccagcgcc                             >  2:945796/1‑150 (MQ=255)
                                                                                                                       aCTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTgccagcgccag                           <  1:1623780/150‑1 (MQ=255)
                                                                                                                         tctGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCa                         <  2:831886/150‑1 (MQ=255)
                                                                                                                           tGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCAtt                       <  2:1207366/150‑1 (MQ=255)
                                                                                                                            gTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTa                      <  1:1031468/150‑1 (MQ=255)
                                                                                                                             ttGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTGGCACCCTGGCAGCGCCAGCATTAg                     <  2:893979/150‑1 (MQ=255)
                                                                                                                             ttGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAg                     <  1:1528834/150‑1 (MQ=255)
                                                                                                                               gggCCAGTTTTTGTCTCTCCGGCACGGATAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCGTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGTCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCa                   <  1:1089094/150‑1 (MQ=255)
                                                                                                                                ggCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTa                      <  2:717259/146‑1 (MQ=255)
                                                                                                                                ggCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTa                      >  1:717259/1‑146 (MQ=255)
                                                                                                                                  ccAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGc                <  1:106059/150‑1 (MQ=255)
                                                                                                                                  ccAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCACTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGc                >  1:128834/1‑150 (MQ=255)
                                                                                                                                       gcaaaactctcCGTGCCGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAggcg           >  2:41808/7‑150 (MQ=255)
                                                                                                                                       gcaaaactctcCGTGCCGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAggcg           >  1:524184/7‑150 (MQ=255)
                                                                                                                                       gcaaaactctcCGTGCCGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAggcg           >  2:1083051/7‑150 (MQ=255)
                                                                                                                                        caaaactctcCGTGCCGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAggcgg          >  2:872688/6‑150 (MQ=255)
                                                                                                                                        caaaactctcCGTGCCGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAggcgg          >  1:323206/6‑150 (MQ=255)
                                                                                                                                         ttGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAggcggc         <  1:1376272/150‑1 (MQ=255)
                                                                                                                                           gTCTCTCCGGCACGGAGAGTTTTGGTGAACTGGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATGGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTa       <  2:1317353/150‑1 (MQ=255)
                                                                                                                                           gTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTa       >  1:1036278/1‑150 (MQ=255)
                                                                                                                                           aactctcCGTGCCGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTa       >  2:1092255/3‑150 (MQ=255)
                                                                                                                                             ctctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTAcg     >  2:205815/1‑150 (MQ=255)
                                                                                                                                               ctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTAcgcg   >  1:1098959/1‑150 (MQ=255)
                                                                                                                                               ctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTAcgcg   <  2:1276449/150‑1 (MQ=255)
                                                                                                                                                tcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGt  <  2:579042/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGT  >  NC_000913/2651983‑2652276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑