Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,705,871 A→T 9.6% V418V (GTT→GTA)  lepA ← elongation factor 4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,705,8710AT 9.6% 242.0 / 13.5 83V418V (GTT→GTA) lepAelongation factor 4
Reads supporting (aligned to +/- strand):  ref base A (35/40);  new base T (5/3);  total (40/43)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

GACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTAC  >  NC_000913/2705725‑2706017
                                                                                                                                                  |                                                                                                                                                  
gaCGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACg                                                                                                                                                 >  1:431518/1‑150 (MQ=255)
gaCGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACg                                                                                                                                                 <  1:31003/150‑1 (MQ=255)
  cGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGtt                                                                                                                                               >  2:169877/1‑150 (MQ=255)
    cATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGc                                                                                                                                             >  1:682060/1‑150 (MQ=255)
    cATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCAGCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACTCCGCGTTTTTCTACGCACAACGTAATAACGTTGc                                                                                                                                             >  2:710302/1‑150 (MQ=255)
     aTAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGcc                                                                                                                                            <  2:844436/150‑1 (MQ=255)
      tAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCg                                                                                                                                           >  2:145340/1‑150 (MQ=255)
       aaCCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTTATTACGTTgtgcg                                                                                                                                          <  1:1474869/150‑5 (MQ=255)
       aaCCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCga                                                                                                                                          >  2:482986/1‑150 (MQ=255)
        aCCACTCGAGGTTGATTTCAGGCGATCGAAGAAGTCGAGCTCCACTTCCGCCATCGGGATCTTGTACGTCAGCGCGACCTGATTATCGTTGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATATCGTTGCCgag                                                                                                                                         <  2:1068777/150‑1 (MQ=255)
        aCCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCgag                                                                                                                                         >  2:103146/1‑150 (MQ=255)
         ccACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTTATTACGTTgtgcgta                                                                                                                                        <  1:1114503/150‑7 (MQ=255)
          cACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGat                                                                                                                                       <  2:1000268/150‑1 (MQ=255)
           aCGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGata                                                                                                                                      <  2:654086/150‑1 (MQ=255)
             gcgAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCTCTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATg                                                                                                                                    <  2:882332/150‑1 (MQ=255)
                 ggTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTg                                                                                                                                >  2:1526210/1‑150 (MQ=255)
                 ggTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTg                                                                                                                                >  1:228943/1‑150 (MQ=255)
                  gTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGc                                                                                                                               <  1:11650/150‑1 (MQ=255)
                   tAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCg                                                                                                                              <  1:169877/150‑1 (MQ=255)
                   tAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCg                                                                                                                              >  2:905550/1‑150 (MQ=255)
                    aGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCgg                                                                                                                             >  2:1157598/1‑150 (MQ=255)
                     gATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgc                                                                                                                            <  1:147451/150‑1 (MQ=255)
                     gATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgc                                                                                                                            <  1:741027/150‑1 (MQ=255)
                     gATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgc                                                                                                                            <  2:628075/150‑1 (MQ=255)
                        ttCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgcagc                                                                                                                         <  2:754700/150‑1 (MQ=255)
                          cAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgcagcag                                                                                                                       <  1:1202403/150‑1 (MQ=255)
                                        agTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTTCCGAGATATGCGTGCGGCAGCAGCATGTGACTCTCTg                                                                                                         <  2:1496421/150‑1 (MQ=255)
                                        agTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTg                                                                                                         >  1:147951/1‑150 (MQ=255)
                                           cGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCaa                                                                                                      >  2:401438/1‑150 (MQ=255)
                                           cGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCaa                                                                                                      >  1:559605/1‑150 (MQ=255)
                                             aGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATc                                                                                                    <  1:1584602/150‑1 (MQ=255)
                                              gCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCg                                                                                                   >  2:193593/1‑150 (MQ=255)
                                               caccacTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCgg                                                                                                  >  1:91164/1‑150 (MQ=255)
                                                accacTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGc                                                                                                 >  1:463801/1‑150 (MQ=255)
                                                   acTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTcg                                                                                              <  2:1632050/150‑1 (MQ=255)
                                                   acTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTcg                                                                                              <  2:91164/150‑1 (MQ=255)
                                                      tCCGGCCTCGGGCTCTCGTAGGTCAGCGACACCTGATTACCGTGGTAAACCATATTGGTCTGCTCGACGCTTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCgcgc                                                                                           <  2:732003/150‑1 (MQ=255)
                                                      tCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCgcgc                                                                                           <  1:591831/150‑1 (MQ=255)
                                                       ccGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCa                                                                                          >  1:629516/1‑150 (MQ=255)
                                                            aTCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgc                                                                                                                            >  1:1013187/1‑111 (MQ=255)
                                                            aTCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgc                                                                                                                            <  2:1013187/111‑1 (MQ=255)
                                                               gggATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAg                                                                                  <  1:874619/150‑1 (MQ=255)
                                                                  aTCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATg                                                                               <  2:228943/150‑1 (MQ=255)
                                                                       gTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTAtt                                                                          >  1:494255/1‑150 (MQ=255)
                                                                       gTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTAtt                                                                          <  1:13327/150‑1 (MQ=255)
                                                                          cGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTAc                                                                       <  1:157922/150‑1 (MQ=255)
                                                                            tCAGCGCCACCTGATTACCGTGGGAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCCATCGGCTCGCGCAGTTCGTAGAGGTTATTTACCg                                                                     >  2:702654/1‑150 (MQ=255)
                                                                             cAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCgc                                                                    <  2:463801/150‑1 (MQ=255)
                                                                                  ccACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCa                                                                                          >  1:348662/1‑123 (MQ=255)
                                                                                  ccACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCa                                                                                          <  2:348662/123‑1 (MQ=255)
                                                                                   cACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCgcaggcag                                                              >  2:208432/1‑150 (MQ=255)
                                                                                       tGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACCTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTg                                                          >  2:37673/1‑150 (MQ=255)
                                                                                          ttACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGAt                                                       <  1:1302710/150‑1 (MQ=255)
                                                                                           tACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATg                                                      <  1:1599079/150‑1 (MQ=255)
                                                                                              cGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGc                                                   <  1:683292/150‑1 (MQ=255)
                                                                                              cGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGc                                                   <  1:482986/150‑1 (MQ=255)
                                                                                               gTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCt                                                  <  2:559605/150‑1 (MQ=255)
                                                                                                    aaaCCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGa                                             <  1:195348/150‑1 (MQ=255)
                                                                                                       ccATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGt                                          >  1:807700/1‑150 (MQ=255)
                                                                                                         atatTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAg                                        <  1:1526210/150‑1 (MQ=255)
                                                                                                              ggTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAAc                                   >  1:1256509/1‑150 (MQ=255)
                                                                                                               gTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACt                                  >  2:694808/1‑150 (MQ=255)
                                                                                                                tCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACtt                                 <  1:193593/150‑1 (MQ=255)
                                                                                                                      cgccgcGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTg                           >  2:1513047/1‑150 (MQ=255)
                                                                                                                        ccgcGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGAc                         >  2:99300/1‑150 (MQ=255)
                                                                                                                             tttttCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGt                    <  2:458483/150‑1 (MQ=255)
                                                                                                                                ttCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCATTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTc                 <  2:949488/150‑1 (MQ=255)
                                                                                                                                ttCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTc                 <  2:682060/150‑1 (MQ=255)
                                                                                                                                 tCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCa                <  1:145340/150‑1 (MQ=255)
                                                                                                                                  atctcggcaACGTTATTACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCaa               >  1:1203155/8‑150 (MQ=255)
                                                                                                                                   tACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCaaa              >  1:1612968/1‑149 (MQ=255)
                                                                                                                                   tACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAAc              >  2:1539054/1‑150 (MQ=255)
                                                                                                                                   tACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAAc              <  2:47615/150‑1 (MQ=255)
                                                                                                                                    ctcggcaACGTTATTACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACt             >  1:1377675/6‑150 (MQ=255)
                                                                                                                                    aCGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACt             >  1:1359841/1‑150 (MQ=255)
                                                                                                                                     tcggcaACGTTATTACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACtt            >  2:1012671/5‑150 (MQ=255)
                                                                                                                                     cGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACtt            <  2:147951/150‑1 (MQ=255)
                                                                                                                                      cggcaACGTTATTACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACt             <  1:768673/145‑1 (MQ=255)
                                                                                                                                      cggcaACGTTATTACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACt             >  2:768673/4‑148 (MQ=255)
                                                                                                                                      cggcaACGTTATTACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTc           >  2:1107449/4‑150 (MQ=255)
                                                                                                                                       cacaACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCa          >  2:55458/1‑150 (MQ=255)
                                                                                                                                        acaACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCAt         >  1:1488197/1‑150 (MQ=255)
                                                                                                                                           aCGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATaca      >  1:1259969/1‑150 (MQ=255)
                                                                                                                                               aataaCGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTAc  <  1:433033/150‑1 (MQ=255)
                                                                                                                                               aataaCGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTAc  <  2:642413/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTAC  >  NC_000913/2705725‑2706017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑