Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,712,128 C→G 6.9% A213P (GCG→CCG)  yfiC ← tRNA m(6)A37 methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,712,1280CG 6.9% 285.2 / 9.4 89A213P (GCG→CCG) yfiCtRNA m(6)A37 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (48/34);  new base G (2/4);  total (50/39)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

AACTGCTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAAT  >  NC_000913/2711981‑2712276
                                                                                                                                                   |                                                                                                                                                    
aaCTGCTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCt                                                                                                                                                    >  1:169686/1‑150 (MQ=255)
 aCTGCTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTg                                                                                                                                                   >  2:1090259/1‑150 (MQ=255)
   tGCTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCg                                                                                                                                                 >  1:1477274/1‑150 (MQ=255)
   tGCTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCg                                                                                                                                                 >  2:1448063/1‑150 (MQ=255)
      tCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCgggg                                                                                                                                              <  1:732894/150‑1 (MQ=255)
         cccATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGgaga                                                                                                                                           <  1:752918/150‑1 (MQ=255)
         cccATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGgaga                                                                                                                                           >  2:205741/1‑150 (MQ=255)
           cATTGCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCATGTGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCCGCAGGCGGGAGAAt                                                                                                                                         <  2:547308/150‑1 (MQ=255)
           cATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAAt                                                                                                                                         >  1:201431/1‑150 (MQ=255)
           cATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAAt                                                                                                                                         >  1:1209278/1‑150 (MQ=255)
            aTTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATg                                                                                                                                        <  1:590001/150‑1 (MQ=255)
            aTTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCCGCAGGCGGGAGAATg                                                                                                                                        <  2:927222/150‑1 (MQ=255)
            aTTCCGGGCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGAACGCTAAAGCATTCTCCCGCCTGCGGGGAGAATg                                                                                                                                        <  1:707792/150‑1 (MQ=255)
             ttCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCACACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGc                                                                                                                                       >  1:399266/1‑150 (MQ=255)
                 ggTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCcagc                                                                                                                                   <  2:1494193/150‑1 (MQ=255)
                 ggTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCcagc                                                                                                                                   <  1:133313/150‑1 (MQ=255)
                  gTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCCGCAGGCGGGAGAATGctttag                                                                                                                                  <  1:1428137/150‑6 (MQ=255)
                    ccGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCCGCAGGCGGGAGAATGctttagcg                                                                                                                                <  1:400598/150‑8 (MQ=255)
                              tttCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCg                                                                                                                      <  2:1622407/150‑1 (MQ=255)
                              tttCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCg                                                                                                                      <  2:1228525/150‑1 (MQ=255)
                                tCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGc                                                                                                                    >  2:1595109/1‑150 (MQ=255)
                                      ggCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGga                                                                                                                                             <  1:1441803/119‑1 (MQ=255)
                                      ggCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGga                                                                                                                                             >  2:1441803/1‑119 (MQ=255)
                                      ggCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTcgc                                                                                                              >  1:1462570/1‑150 (MQ=255)
                                        cAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCgcgc                                                                                                            >  2:488086/1‑150 (MQ=255)
                                                   tAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCg                                                                                                 >  1:717618/1‑150 (MQ=255)
                                                   tAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCg                                                                                                 <  1:92600/150‑1 (MQ=255)
                                                   tAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCg                                                                                                 >  2:1454502/1‑150 (MQ=255)
                                                   tAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCg                                                                                                 <  1:488086/150‑1 (MQ=255)
                                                      aCAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCca                                                                                              <  1:228187/150‑1 (MQ=255)
                                                          aTAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACAtc                                                                                          >  2:501142/1‑150 (MQ=255)
                                                            aaaaaGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACAtccg                                                                                        >  2:987991/1‑150 (MQ=255)
                                                            aaaaaGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACAtccg                                                                                        >  1:1280502/1‑150 (MQ=255)
                                                            aaaaaGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACAtccg                                                                                        >  2:789755/1‑150 (MQ=255)
                                                             aaaaGCCTGGGTCAGCGCAGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACAtccgt                                                                                       >  1:1334509/1‑150 (MQ=255)
                                                              aaaGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTCGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCCTTTTCCGCCACAtccgtc                                                                                      >  1:871798/1‑150 (MQ=255)
                                                                aGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACAtccgtccg                                                                                    <  1:789755/150‑1 (MQ=255)
                                                                 gCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGc                                                                                   <  2:201431/150‑1 (MQ=255)
                                                                  ccTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCa                                                                                  >  1:824142/1‑150 (MQ=255)
                                                                      ggTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACg                                                                              >  1:1508366/1‑150 (MQ=255)
                                                                      ggTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCACACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACg                                                                              <  2:399266/150‑1 (MQ=255)
                                                                        tCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTa                                                                            <  2:340725/150‑1 (MQ=255)
                                                                            cgcCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATg                                                                        <  1:1454502/150‑1 (MQ=255)
                                                                               cGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACAtccgtccg                                                                                    >  2:1157221/1‑135 (MQ=255)
                                                                               cGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACAtccgtccg                                                                                    <  1:1157221/135‑1 (MQ=255)
                                                                                      gCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATg                                                              >  2:575016/1‑150 (MQ=255)
                                                                                      gCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATg                                                              >  2:945573/1‑150 (MQ=255)
                                                                                        ttCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCt                                                            <  1:318549/150‑1 (MQ=255)
                                                                                               tAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGcc                                                     <  2:824142/150‑1 (MQ=255)
                                                                                                 gTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAg                                                   >  2:363233/1‑150 (MQ=255)
                                                                                                  ttCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGc                                                  >  1:1441570/1‑150 (MQ=255)
                                                                                                    ctggtctggCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGc                                                             >  1:1090916/1‑137 (MQ=255)
                                                                                                    ctggtctggCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGc                                                             <  2:1090916/137‑1 (MQ=255)
                                                                                                      ggtctggCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCg                                              >  2:45633/1‑150 (MQ=255)
                                                                                                       gtctggCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGt                                             >  1:1233388/1‑150 (MQ=255)
                                                                                                       gtctggCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGt                                             >  2:506776/1‑150 (MQ=255)
                                                                                                        tctggCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTa                                            <  1:1090259/150‑1 (MQ=255)
                                                                                                           ggCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTaaaa                                         >  2:905086/1‑150 (MQ=255)
                                                                                                           ggCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTaaaa                                         <  1:1595109/150‑1 (MQ=255)
                                                                                                           ggCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTaaaa                                         >  2:521064/1‑150 (MQ=255)
                                                                                                              ccACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATCACAGCCCATGCTTAATGCCAGCTCCGTAAAACCa                                      >  2:83522/1‑150 (MQ=255)
                                                                                                                  gAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTAc                                  <  1:987991/150‑1 (MQ=255)
                                                                                                                  gAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTAc                                  <  1:935550/150‑1 (MQ=255)
                                                                                                                   aaTGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCACCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTAcc                                 <  2:68395/150‑1 (MQ=255)
                                                                                                                   aaTGACTAAGCGATCGCTAAAGCATTCTCCCGCCAGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTAcc                                 <  2:1296727/150‑1 (MQ=255)
                                                                                                                        cTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCt                            >  1:504922/1‑150 (MQ=255)
                                                                                                                        cTAAGAGATCGCTAAAGCATTCTCCAGCCTGCGGGGAGAATGCAAGCAGCACCCGATGCGGAAGTCGAGCTTCGTTTTAAGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCAAGCTCAGTAAAACCATTACCAATCt                            >  2:794081/1‑150 (MQ=255)
                                                                                                                              gATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTAc                                  <  1:528388/138‑1 (MQ=255)
                                                                                                                              gATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTAc                                  >  2:528388/1‑138 (MQ=255)
                                                                                                                                 cGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGgca                   >  1:860436/1‑150 (MQ=255)
                                                                                                                                  gCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGgcag                  <  2:1462570/150‑1 (MQ=255)
                                                                                                                                   cTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGgcagc                 <  1:501142/150‑1 (MQ=255)
                                                                                                                                   cTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGgcagc                 >  1:1262000/1‑150 (MQ=255)
                                                                                                                                   cTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGgcagc                 >  2:660194/1‑150 (MQ=255)
                                                                                                                                     ctggCATTCTCCCCGCAGGCGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCAc               >  2:475760/4‑150 (MQ=255)
                                                                                                                                     aaaGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAATTGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCTGCAc               <  2:169686/150‑1 (MQ=255)
                                                                                                                                     aaaGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCAc               <  2:233662/150‑1 (MQ=255)
                                                                                                                                       aGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTa             >  2:532657/1‑150 (MQ=255)
                                                                                                                                         cATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACg           >  2:1103577/1‑150 (MQ=255)
                                                                                                                                         cATTCTCCCCGCAGGCGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACg           >  1:1312100/1‑150 (MQ=255)
                                                                                                                                          aTTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGc          >  1:1337949/1‑150 (MQ=255)
                                                                                                                                           ttCTCCCGTCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCa         <  1:555679/150‑1 (MQ=255)
                                                                                                                                           ttCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCa         >  2:405407/1‑150 (MQ=255)
                                                                                                                                              tcCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGaa      >  2:123752/1‑150 (MQ=255)
                                                                                                                                              tcCCGCCTGCGGGGAGAATGCCAGCAGCACCCAATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGaa      >  1:1457414/1‑150 (MQ=255)
                                                                                                                                               cccGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGaaa     >  2:761365/1‑150 (MQ=255)
                                                                                                                                               cccGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGaaa     <  1:930805/150‑1 (MQ=255)
                                                                                                                                                 cGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGaaaaa   >  2:1125315/1‑150 (MQ=255)
                                                                                                                                                  gCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAAt  <  1:521064/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AACTGCTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAAT  >  NC_000913/2711981‑2712276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑