Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,752,758 T→A 6.2% L322M (TTG→ATG)  recN → DNA repair protein RecN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,752,7580TA 6.2% 245.3 / 3.8 79L322M (TTG→ATG) recNDNA repair protein RecN
Reads supporting (aligned to +/- strand):  ref base T (40/34);  new base A (2/3);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCTACCATCCAGATTGCTGAAGCCAGCGATGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAATCGCGCGCGCATTACACCAACAACGCCAGCAATA  >  NC_000913/2752609‑2752906
                                                                                                                                                     |                                                                                                                                                    
aagCTACCATCCAGATTGCTGAAGCCAGCGATGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCAt                                                                                                                                                      >  2:665778/1‑150 (MQ=255)
  gCTACCATCCAGATTGCTGAAGCCAGCGATGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTg                                                                                                                                                    <  2:161364/150‑1 (MQ=255)
    tACCATCCAGATTGCTGAAGCCAGCGATGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTTGCACTTAAACATCACGTCAGCCATTAGGCATTGcc                                                                                                                                                  <  2:676837/150‑1 (MQ=255)
    aaCCATACAGATTCCTGAAGCCAGCGATGAACTGCGCCAATACAGCGATACTCTGTATCTCTATCACAACAGACTATTTGAAATTGAACAGCGCATCTCAAAACACAATTCGCTCGCACGTAAACATCACGTCAGCCCTCAGTCAATGcc                                                                                                                                                  <  2:666045/149‑1 (MQ=255)
            aGATTGCTGAAGCCAGCGATGAACTGCGCCTCTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTAtt                                                                                                                                          >  2:256018/1‑150 (MQ=255)
            aGATTGCTGAAGCCAGCGATGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTAtt                                                                                                                                          >  2:1249977/1‑150 (MQ=255)
             gATTGCTGAAGCCAGCGATGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTa                                                                                                                                         >  1:1510877/1‑150 (MQ=255)
             gATTGCTGAAGCCAGCGATGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCACTGTGGCAATGCCTCAgggctg                                                                                                                                         <  1:285777/150‑6 (MQ=255)
             gATTGCTGAACCCAGCGATGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTa                                                                                                                                         <  2:1080510/150‑1 (MQ=255)
                  cTGAAGCCAGCGATGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGt                                                                                                                                    >  1:1629457/1‑150 (MQ=255)
                   tGAAGCCAGCGATGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTc                                                                                                                                   >  2:562779/1‑150 (MQ=255)
                       gCCAGCGATGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTa                                                                                                                               >  2:1491054/1‑150 (MQ=255)
                        ccAGCGATGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTAc                                                                                                                              <  2:762395/150‑1 (MQ=255)
                         cAGCGATGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACt                                                                                                                             >  2:1491007/1‑150 (MQ=255)
                            cGATGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGga                                                                                                                          <  1:249676/150‑1 (MQ=255)
                             gATGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGgaa                                                                                                                         >  2:396351/1‑150 (MQ=255)
                              attaaCTGCGCCACTACTGCGATCGTCTGGATCTCTATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGTCACGTAAACATCACGTCATCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGgaag                                                                                                                        <  1:187344/147‑1 (MQ=255)
                               tGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGgaaga                                                                                                                       <  1:778199/150‑1 (MQ=255)
                                   cTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAAcag                                                                                                                   >  1:49106/1‑150 (MQ=255)
                                        ccACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAgcagca                                                                                                              >  1:1252696/1‑150 (MQ=255)
                                        ccACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAgcagca                                                                                                              <  1:858738/150‑1 (MQ=255)
                                        ccACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGCCGCTACTGGAAGAACAgcagca                                                                                                              >  2:205850/1‑150 (MQ=255)
                                              ctGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGa                                                                                                        >  1:485374/1‑150 (MQ=255)
                                                    tCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCa                                                                                                  <  2:1547413/150‑1 (MQ=255)
                                                    tCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCa                                                                                                  <  1:627735/150‑1 (MQ=255)
                                                      gTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAgg                                                                                                <  1:665778/150‑1 (MQ=255)
                                                        cTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGcc                                                                                              >  2:275909/1‑150 (MQ=255)
                                                          ggATCTCGATCACAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCAGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGa                                                                                            >  2:274149/1‑150 (MQ=255)
                                                           gATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGAc                                                                                           <  2:1268399/150‑1 (MQ=255)
                                                           gATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGAc                                                                                           >  1:1426956/1‑150 (MQ=255)
                                                                 gATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACaa                                                                                     >  2:1505322/1‑150 (MQ=255)
                                                                           gACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTg                                                                           <  1:256018/150‑1 (MQ=255)
                                                                              tATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTgcgc                                                                        >  1:553180/1‑150 (MQ=255)
                                                                               aTTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCt                                                                       >  1:564243/1‑150 (MQ=255)
                                                                                tttGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCAGACTCACAAGAAACGCTTGCGCTg                                                                      >  1:735616/1‑150 (MQ=255)
                                                                                  tgaacttgaacAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACACCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGc                                                                    <  1:1040983/150‑1 (MQ=255)
                                                                                   gaacttgaacAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGgaa                                                                                                                         <  2:1538505/96‑1 (MQ=255)
                                                                                   gaacttgaacAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGgaa                                                                                                                         >  1:1538505/1‑96 (MQ=255)
                                                                                    aacttgaacAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCgg                                                                  <  2:816606/150‑1 (MQ=255)
                                                                                     acttgaacAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGt                                                                 >  1:856659/1‑150 (MQ=255)
                                                                                      cttgaacAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTa                                                                >  1:699383/1‑150 (MQ=255)
                                                                                       ttgaacAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTaa                                                               <  1:912885/150‑1 (MQ=255)
                                                                                         gaacAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACg                                                             >  2:37456/1‑150 (MQ=255)
                                                                                         gaacAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACg                                                             >  2:1523162/1‑150 (MQ=255)
                                                                                           acAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGaa                                                           <  2:49106/150‑1 (MQ=255)
                                                                                           acAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGaa                                                           <  1:1491054/150‑1 (MQ=255)
                                                                                            cAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGaaa                                                          <  2:705565/150‑1 (MQ=255)
                                                                                             aGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAAc                                                         <  1:102973/150‑1 (MQ=255)
                                                                                             aGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAAc                                                         <  1:1176766/150‑1 (MQ=255)
                                                                                             aGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAAc                                                         <  2:256505/150‑1 (MQ=255)
                                                                                              gcgcATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAAca                                                        >  1:1462186/1‑150 (MQ=255)
                                                                                                  aTCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAAcatcat                                                    <  1:262695/150‑1 (MQ=255)
                                                                                                   tctcAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACatcatc                                                   <  2:1243867/150‑1 (MQ=255)
                                                                                                     tcAAAACAGATTTCGCTGGCACGTAAACATCATGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATcag                                                 >  1:1167781/1‑150 (MQ=255)
                                                                                                        aaaCAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATcagcag                                              >  2:163875/1‑150 (MQ=255)
                                                                                                         aaCAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAgg                                             <  1:1491007/150‑1 (MQ=255)
                                                                                                           cAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCa                                           >  2:77402/1‑150 (MQ=255)
                                                                                                               tttCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTgg                                       >  2:732845/1‑150 (MQ=255)
                                                                                                                ttCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGa                                      <  2:1426956/150‑1 (MQ=255)
                                                                                                                  cGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGaaa                                    <  1:1584737/150‑1 (MQ=255)
                                                                                                                  cGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTCGaaa                                    >  2:643343/1‑150 (MQ=255)
                                                                                                                   gCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAAt                                   <  2:1382540/150‑1 (MQ=255)
                                                                                                                        cACGTAAACATCACGTCAGCCCTGCGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGACCAGCAGCAACTGGCCGATCAGGCCGACTCCCAAGAAACGCTTGCGCTGGCGGTACCGCAACCTCCTCAGAAGGCACTGGATATcgcgc                              >  2:1438398/1‑150 (MQ=255)
                                                                                                                        cACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAATcgcgc                              >  2:53107/1‑150 (MQ=255)
                                                                                                                         aCGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAATcgcgcg                             <  2:1078066/150‑1 (MQ=255)
                                                                                                                          cGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAATcgcgcgc                            >  2:108118/1‑150 (MQ=255)
                                                                                                                              aaCATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAATCGCGCGCGCAt                        <  2:789747/150‑1 (MQ=255)
                                                                                                                                cATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAATCGCGCGCGCATTa                      >  1:863250/1‑150 (MQ=255)
                                                                                                                                cATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAATCGCGCGCGCATTa                      >  1:1541648/1‑150 (MQ=255)
                                                                                                                                 aTCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAATCGCGCGCGCATTac                     >  2:1250234/1‑150 (MQ=255)
                                                                                                                                 aTCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAATCGCGCGCGCATTac                     <  1:275909/150‑1 (MQ=255)
                                                                                                                                   cACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGCAACATCATCAGCAGGCACTGGAAATCGCGCGCGCATTacac                   >  2:887364/1‑150 (MQ=255)
                                                                                                                                       tCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAATCGCGCGCGCATTACACcaac               <  2:1629457/150‑1 (MQ=255)
                                                                                                                                       tCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAATCGCGCGCGCATTACACcaac               >  2:1139230/1‑150 (MQ=255)
                                                                                                                                       gtaatacTGTGGCAATGCCTCAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTgg                                       >  2:1076167/7‑126 (MQ=255)
                                                                                                                                       gtaatacTGTGGCAATGCCTCAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTgg                                       <  1:1076167/120‑1 (MQ=255)
                                                                                                                                           cccTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAATCGCGCGCGCATTACACCAACAACg           >  1:56444/1‑150 (MQ=255)
                                                                                                                                            acTGTGGCAATGCCTCAGTATTACCAGTCGCTAATGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAATCGCGCGCGCATTACACCAACAACGc          >  2:1266864/2‑150 (MQ=255)
                                                                                                                                                 ggCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAATCGCGCGCGCATTACACCAACAACGCCAGCa     <  1:1250234/150‑1 (MQ=255)
                                                                                                                                                    aTTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAATCGCGCGCGCATTACACCAACAACGCCAGCAata  >  1:40863/1‑150 (MQ=255)
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AAGCTACCATCCAGATTGCTGAAGCCAGCGATGAACTGCGCCACTACTGCGATCGTCTGGATCTCGATCCCAACCGACTATTTGAACTTGAACAGCGCATCTCAAAACAGATTTCGCTGGCACGTAAACATCACGTCAGCCCTGAGGCATTGCCACAGTATTACCAGTCGCTACTGGAAGAACAGCAGCAACTGGACGATCAGGCCGACTCACAAGAAACGCTTGCGCTGGCGGTAACGAAACATCATCAGCAGGCACTGGAAATCGCGCGCGCATTACACCAACAACGCCAGCAATA  >  NC_000913/2752609‑2752906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑