Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,771,183 A→C 7.7% A12A (GCA→GCC)  yfjT → CP4‑57 prophage; uncharacterized protein YfjT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,771,1830AC 7.7% 270.5 / 13.2 91A12A (GCA→GCC) yfjTCP4‑57 prophage; uncharacterized protein YfjT
Reads supporting (aligned to +/- strand):  ref base A (41/43);  new base C (3/4);  total (44/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

GTATACGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTAT  >  NC_000913/2771034‑2771332
                                                                                                                                                     |                                                                                                                                                     
gTATACGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCa                                                                                                                                                       <  2:499726/150‑1 (MQ=255)
  ataCGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAg                                                                                                                                                     <  1:926902/150‑1 (MQ=255)
     cGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTAc                                                                                                                                                  >  2:179706/1‑150 (MQ=255)
       aaagaaaAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACaa                                                                                                                                                >  2:312238/1‑150 (MQ=255)
          gaaaAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTTGCCAGTACaaacc                                                                                                                                             <  2:311713/150‑4 (MQ=255)
           aaaaaTACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTTGCCAGTACaaaccg                                                                                                                                            <  1:746964/150‑5 (MQ=255)
           aaaaaTACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTTGCCAGTACaaaccg                                                                                                                                            <  2:49027/150‑5 (MQ=255)
           aaaaaTACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGtt                                                                                                                                            >  2:610609/1‑150 (MQ=255)
            aaaaTACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTTGCCAGTACaaaccgg                                                                                                                                           <  1:803547/150‑6 (MQ=255)
             aaaTACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCg                                                                                                                                          >  2:1058012/1‑150 (MQ=255)
             aaaTACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCg                                                                                                                                          <  1:859835/150‑1 (MQ=255)
               aTACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGcc                                                                                                                                        <  1:94808/150‑1 (MQ=255)
                tACTTCGAATAATCCCATGACGGCAAACAGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGCGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCa                                                                                                                                       >  2:1124162/1‑150 (MQ=255)
                  cTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGc                                                                                                                                     <  1:435287/150‑1 (MQ=255)
                  cTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGc                                                                                                                                     >  2:1009601/1‑150 (MQ=255)
                  cTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGc                                                                                                                                     >  2:1066731/1‑150 (MQ=255)
                     cGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCttt                                                                                                                                  <  1:288323/150‑1 (MQ=255)
                      gAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTa                                                                                                                                 >  1:1392542/1‑150 (MQ=255)
                        atatTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACa                                                                                                                               <  2:1531511/150‑1 (MQ=255)
                             cccATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCctc                                                                                                                          >  2:248573/1‑150 (MQ=255)
                                aTGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGc                                                                                                                       <  1:975291/150‑1 (MQ=255)
                                aTGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAAACGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGc                                                                                                                       <  2:1243269/150‑1 (MQ=255)
                                 tGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCg                                                                                                                      <  1:550320/150‑1 (MQ=255)
                                    cGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGAAATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTc                                                                                                                   >  2:1331661/1‑150 (MQ=255)
                                       cAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTcccc                                                                                                                <  1:179706/150‑1 (MQ=255)
                                       cAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTcccc                                                                                                                <  1:248573/150‑1 (MQ=255)
                                       cAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTcccc                                                                                                                >  1:413672/1‑150 (MQ=255)
                                         aaCTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAAGGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCgg                                                                                                              <  1:309196/150‑1 (MQ=255)
                                         aaCTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCgg                                                                                                              <  1:1062223/150‑1 (MQ=255)
                                           cTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGAc                                                                                                            <  2:1473446/150‑1 (MQ=255)
                                               tGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTg                                                                                                        >  1:1479183/1‑150 (MQ=255)
                                               tGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTg                                                                                                        >  1:1473374/1‑150 (MQ=255)
                                               tGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTg                                                                                                        >  2:659352/1‑150 (MQ=255)
                                               tGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTg                                                                                                        >  1:1041746/1‑150 (MQ=255)
                                               tGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTg                                                                                                        <  1:1009601/150‑1 (MQ=255)
                                                 ggACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGc                                                                                                      <  2:561449/150‑1 (MQ=255)
                                                             aTACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACg                                                                                          >  1:1443782/1‑150 (MQ=255)
                                                             aTACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACg                                                                                          <  1:1352800/150‑1 (MQ=255)
                                                              tACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACgg                                                                                         <  2:1278797/150‑1 (MQ=255)
                                                              tACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACgg                                                                                         <  1:543328/150‑1 (MQ=255)
                                                                ccGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTc                                                                                       >  1:1036545/1‑150 (MQ=255)
                                                                ccGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTc                                                                                       >  1:174706/1‑150 (MQ=255)
                                                                ccGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTc                                                                                       >  1:537141/1‑150 (MQ=255)
                                                                     aaaCCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGc                                                                                  >  2:1358144/1‑150 (MQ=255)
                                                                       aCCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCC‑GACTCTGGCAACAGGGGTACGGTCAGGGCAAt                                                                               >  1:452128/1‑150 (MQ=255)
                                                                           tGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCg                                                                            <  1:659352/150‑1 (MQ=255)
                                                                            gATACCCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCgg                                                                           >  2:602805/1‑150 (MQ=255)
                                                                              tAACCGGGAATAGAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGaa                                                                         <  2:291703/150‑1 (MQ=255)
                                                                                aCCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATa                                                                       >  1:230843/1‑150 (MQ=255)
                                                                                   gggAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACaa                                                                    >  1:726768/1‑149 (MQ=255)
                                                                                   gggAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAg                                                                    <  1:759146/150‑1 (MQ=255)
                                                                                    ggAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAgt                                                                   <  1:1440351/150‑1 (MQ=255)
                                                                                      aaTAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAgtgt                                                                 <  2:1345862/150‑1 (MQ=255)
                                                                                          aaaaCAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACg                                                             >  1:770069/1‑150 (MQ=255)
                                                                                          aaaaCAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACg                                                             >  2:1559958/1‑150 (MQ=255)
                                                                                          aaaaCAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACg                                                             >  2:633191/1‑150 (MQ=255)
                                                                                            aacaacGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGa                                                           <  1:1358144/150‑1 (MQ=255)
                                                                                                acGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGcc                                                       <  1:312238/150‑1 (MQ=255)
                                                                                                 cGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGc                                                                                                      <  2:1275291/102‑1 (MQ=255)
                                                                                                 cGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGc                                                                                                      >  1:1275291/1‑102 (MQ=255)
                                                                                                   agaAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGc                                                    >  1:17810/1‑150 (MQ=255)
                                                                                                    gaAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCg                                                   >  2:54097/1‑150 (MQ=255)
                                                                                                    gaAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCg                                                   <  1:1124162/150‑1 (MQ=255)
                                                                                                       aGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGaa                                                <  1:1058012/150‑1 (MQ=255)
                                                                                                         gagaCATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGTTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGTCAATTCGGAATACAGTGTGACGTATGCCAGCGGAAAg                                              <  2:252746/150‑1 (MQ=255)
                                                                                                            aCATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATg                                           <  2:1036545/150‑1 (MQ=255)
                                                                                                                aaaTGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTa                                       >  2:1238223/1‑150 (MQ=255)
                                                                                                                 aaTGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTAc                                      >  1:756304/1‑150 (MQ=255)
                                                                                                                 aaTGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTAc                                      <  1:22224/150‑1 (MQ=255)
                                                                                                                      aaaTACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATcg                                 <  2:468537/150‑2 (MQ=255)
                                                                                                                          aCGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTg                             <  2:1443782/150‑1 (MQ=255)
                                                                                                                          aCGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTg                             <  1:1269702/150‑1 (MQ=255)
                                                                                                                            gTTCACTAAGCCGGTTTGTACTGGAAAGTACAATGTTCGCCAGCGTTACAGTCTCTGCGGTCCCCGGACTCTTTCAACAGGGGTACGGTCCGGGCAATGCTTAATACAGGGGGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCa                           <  2:1380701/150‑1 (MQ=255)
                                                                                                                             ttCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCAc                          <  1:619940/150‑1 (MQ=255)
                                                                                                                              tCACTAAGCCGGTTTGTAATGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACg                         <  2:1046651/150‑1 (MQ=255)
                                                                                                                                aCTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACggg                       >  1:1158259/1‑150 (MQ=255)
                                                                                                                                aCTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCACCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACggg                       >  2:1572808/1‑150 (MQ=255)
                                                                                                                                 cTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGa                      >  1:433970/1‑150 (MQ=255)
                                                                                                                                    gcgaacattGTACTTGCCAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAAt                   >  2:4743/8‑150 (MQ=255)
                                                                                                                                     gCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATc                  >  1:332453/1‑150 (MQ=255)
                                                                                                                                     cgaacattGTACTTGCCAGTACAATGTTCGCCAGGTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATc                  >  1:684631/7‑150 (MQ=255)
                                                                                                                                      ccGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATcc                 >  1:762596/1‑150 (MQ=255)
                                                                                                                                        ggTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCgg               <  2:174706/150‑1 (MQ=255)
                                                                                                                                        acattGTACTTGCCAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGCCTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCgg               >  2:1593695/4‑150 (MQ=255)
                                                                                                                                          tttGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGAc             <  2:1479183/150‑1 (MQ=255)
                                                                                                                                          tttGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGAc             <  2:1473374/150‑1 (MQ=255)
                                                                                                                                            tGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCa           >  2:972918/1‑150 (MQ=255)
                                                                                                                                             gTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCTGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGTGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAg          <  2:556062/150‑1 (MQ=255)
                                                                                                                                             gTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACAATCAACTGCACGGGAAATCCGGACCac          >  2:554122/1‑149 (MQ=255)
                                                                                                                                                cTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAAc       >  2:138823/1‑150 (MQ=255)
                                                                                                                                                cTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAAc       >  2:1224488/1‑150 (MQ=255)
                                                                                                                                                  ggCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACgg     <  1:633191/150‑1 (MQ=255)
                                                                                                                                                     aaGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTAt  >  2:475742/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTATACGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTAT  >  NC_000913/2771034‑2771332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑