Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,908,486	G→C	6.8%	A394G (GCC→GGC)	pyrG ←	CTP synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,908,486	0	G	C	6.8%	227.8 / 4.4	71	A394G (GCC→GGC)	pyrG	CTP synthetase
Reads supporting (aligned to +/- strand):  ref base G (30/36);  new base C (3/2);  total (33/38)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGCCGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACGAGGATTGCGTCC  >  NC_000913/2908338‑2908635                                                                                                                                                     |                                                                                                                                                      tgCGCCGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGc                                                                                                                                                      <  1:203541/150‑1 (MQ=255)        agaCGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATgg                                                                                                                                               <  1:590232/150‑1 (MQ=255)         gaCGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGc                                                                                                                                              <  2:491760/150‑1 (MQ=255)           cGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGa                                                                                                                                            <  2:594007/150‑1 (MQ=255)           cGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGa                                                                                                                                            <  1:664204/150‑1 (MQ=255)             cATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGc                                                                                                                                          >  1:807555/1‑150 (MQ=255)              aTGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCg                                                                                                                                         <  2:872392/150‑1 (MQ=255)              aTGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCg                                                                                                                                         <  1:754641/150‑1 (MQ=255)                ggTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTa                                                                                                                                       >  1:623942/1‑150 (MQ=255)                   aCCGCCGAGATCGCTCTTCTCGCTACGAACTTCATCGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGCCAACATGGAGAACgccaac                                                                                                                                    <  2:322427/150‑6 (MQ=255)                   aCCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATc                                                                                                                                    <  2:427677/150‑1 (MQ=255)                   aCCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATc                                                                                                                                    <  1:1216558/150‑1 (MQ=255)                          agaTCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAAc                                                                                                                             <  1:597259/150‑1 (MQ=255)                           gaTCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACg                                                                                                                            <  2:321962/150‑1 (MQ=255)                           gaTCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAAAATGGCGAGCGTAATCAATTAACg                                                                                                                            >  2:728920/1‑150 (MQ=255)                            aTCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGc                                                                                                                           >  2:1226124/1‑150 (MQ=255)                                       cGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATAc                                                                                                                <  2:409813/150‑1 (MQ=255)                                       cGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATAc                                                                                                                <  2:1040230/150‑1 (MQ=255)                                       cGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATAc                                                                                                                >  1:1088351/1‑150 (MQ=255)                                        gCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATAcc                                                                                                               >  1:898310/1‑150 (MQ=255)                                         cTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATAccc                                                                                                              >  1:468317/1‑150 (MQ=255)                                            cGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTTGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAgg                                                                                                           <  2:1400760/150‑1 (MQ=255)                                            cGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAgg                                                                                                           <  2:623942/150‑1 (MQ=255)                                              aaCTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCa                                                                                                         <  1:1370241/150‑1 (MQ=255)                                                cTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCaaa                                                                                                       <  1:341443/150‑1 (MQ=255)                                                cTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCaaa                                                                                                       >  1:1150579/1‑150 (MQ=255)                                                 ttCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAAt                                                                                                      >  1:1463358/1‑150 (MQ=255)                                                  tCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATg                                                                                                     >  1:37825/1‑150 (MQ=255)                                                    aaCGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAATCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGcc                                                                                                   <  1:1309344/150‑1 (MQ=255)                                                      cGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCa                                                                                                 <  1:659584/150‑1 (MQ=255)                                                         tGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGAt                                                                                              <  2:1085850/150‑1 (MQ=255)                                                             gTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAgg                                                                                          >  1:433444/1‑150 (MQ=255)                                                             gTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAgg                                                                                          <  2:430918/150‑1 (MQ=255)                                                                  cATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAatat                                                                                     >  2:567914/1‑150 (MQ=255)                                                                       cgcCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTtc                                                                                <  2:35616/150‑1 (MQ=255)                                                                       cgcCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTtc                                                                                <  1:920985/150‑1 (MQ=255)                                                                        gcCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGGTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTtct                                                                               <  2:1290729/150‑1 (MQ=255)                                                                              cGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCAcgcg                                                                         >  1:1445623/1‑150 (MQ=255)                                                                                gTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCa                                                                       <  1:1296925/150‑1 (MQ=255)                                                                                      aGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAAcgc                                                                 >  1:900213/1‑150 (MQ=255)                                                                                      aGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAAcgc                                                                 >  1:1535689/1‑150 (MQ=255)                                                                                      aGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAAcgc                                                                 >  2:1297838/1‑150 (MQ=255)                                                                                           cacaACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGt                                                            <  2:626239/150‑1 (MQ=255)                                                                                           cacaACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGt                                                            >  1:1569125/1‑150 (MQ=255)                                                                                           cacaACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATACTGTTCTCACGCGCAAAACGCGCGGt                                                            >  1:669066/1‑150 (MQ=255)                                                                                             caACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCg                                                          <  2:382871/150‑1 (MQ=255)                                                                                             caACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCg                                                          <  1:728920/150‑1 (MQ=255)                                                                                                     tACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATg                                                  <  1:1219043/150‑1 (MQ=255)                                                                                                         tACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCtt                                              >  1:433685/1‑150 (MQ=255)                                                                                                           cAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCt                                            >  1:43444/1‑150 (MQ=255)                                                                                                                tGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGcc                                       <  2:1150579/150‑1 (MQ=255)                                                                                                                   cacaAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACg                                    >  2:971113/1‑150 (MQ=255)                                                                                                                        aTTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGc                               >  1:1246781/1‑150 (MQ=255)                                                                                                                          tCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCg                             >  1:412039/1‑150 (MQ=255)                                                                                                                          tCCGTAGAGTTGGCGTTCTCCATGTTGCCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATCCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGAAATCATGCCTTCTACGCCACGATAGCCg                             <  2:553951/150‑1 (MQ=255)                                                                                                                            cGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGGGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCAAGATAGCCGaa                           <  2:18417/150‑1 (MQ=255)                                                                                                                               agagTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAAcc                        <  2:1126781/150‑1 (MQ=255)                                                                                                                                gagTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCg                       >  2:318329/1‑150 (MQ=255)                                                                                                                                    tGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAg                   >  1:913633/1‑150 (MQ=255)                                                                                                                                     ggCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAgg                  >  2:849515/1‑150 (MQ=255)                                                                                                                                       cgctcgccATGTTGCCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTa                >  1:137849/7‑150 (MQ=255)                                                                                                                                       cGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTa                <  2:468317/150‑1 (MQ=255)                                                                                                                                         ctcgccATGTTGCCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACg              >  1:1193866/5‑150 (MQ=255)                                                                                                                                          tcgccATGTTGCCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACGa             >  2:1251332/4‑150 (MQ=255)                                                                                                                                           ctcCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCTAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACGAg            >  2:886492/1‑150 (MQ=255)                                                                                                                                             ccATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACGAGGa          >  2:1180346/1‑150 (MQ=255)                                                                                                                                               aTGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACGAGGAtt        >  1:1397624/1‑150 (MQ=255)                                                                                                                                               aTGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACGAGGAtt        <  2:1025210/150‑1 (MQ=255)                                                                                                                                                tGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCAc                                     >  1:916583/1‑120 (MQ=255)                                                                                                                                                tGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCAc                                     <  2:916583/120‑1 (MQ=255)                                                                                                                                                tGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACGAGGATTg       <  1:831612/150‑1 (MQ=255)                                                                                                                                                   atgCAACAATGCGAGCGTAATCAATTAACGCAACCTGAATACCCAGGCAAATCCCCAGATAACGAATATTGTTCTCACGCGCAAAACCCGCGGTAGTAATCATGCCTTCTACACAACGATAGCCGAAACCGCCAGGTACGACGATTGCGt    >  2:681488/3‑150 (MQ=255)                                                                                                                                                    ggCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACGAGGATTGCGTc   >  1:493850/1‑150 (MQ=255)                                                                                                                                                    ggCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACGAGGATTGCGTc   >  1:309777/1‑150 (MQ=255)                                                                                                                                                    ggCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACGAGGATTGCGTc   >  1:155319/1‑150 (MQ=255)                                                                                                                                                     gCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAACCGAAACCGCCAGGTACGAGGATTGCGTcc  <  2:210977/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                      TGCGCCGAGACGCATGGTACCGCCGAGATCGCTCTTCTCGCTACGAACTTCAACGTTGCCGTTTTCATCGCGCCACTCGGTAATCAGCGCCACAACCGGGTACTTACAGTCTGGCACAAATTCCGTAGAGTTGGCGTTCTCCATGTTGGCAACATGGCGAGCGTAATCAATTAACGCCACCTGCATACCCAGGCAAATGCCCAGATAAGGAATATTGTTCTCACGCGCAAAACGCGCGGTCGTAATCATGCCTTCTACGCCACGATAGCCGAAACCGCCAGGTACGAGGATTGCGTCC  >  NC_000913/2908338‑2908635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑