BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,090,229	G→A	10.4%	G159S (GGT→AGT)	yggI →	conserved protein YggI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,090,229	0	G	A	10.4%	323.9 / 21.6	115	G159S (GGT→AGT)	yggI	conserved protein YggI
Reads supporting (aligned to +/- strand): ref base G (47/56); new base A (4/8); total (51/64)
Fisher's exact test for biased strand distribution p-value = 5.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-01

Only 100 of 121 total aligned reads displayed.

TTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCTATTGCTGCGGTGGT  >  NC_000913/3090080‑3090378

ttCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCg                                                                                                                                                       <  2:932956/150‑1 (MQ=255)

 tCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCgg                                                                                                                                                      <  2:1473751/150‑1 (MQ=255)

 tCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCACTGTGTTCACTGCgg                                                                                                                                                      >  2:67731/1‑150 (MQ=255)

 tCCCGCCCGTCGTACGCATCAGGTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCgg                                                                                                                                                      <  1:853735/150‑1 (MQ=255)

 tCCCGCCCGGCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCgg                                                                                                                                                      <  2:1391625/150‑1 (MQ=255)

  cccgcccgTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGt                                                                                                                                                     >  1:995935/1‑150 (MQ=255)

   ccgcccgTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTg                                                                                                                                                    <  1:995847/150‑1 (MQ=255)

   ccgcccgTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTg                                                                                                                                                    >  2:840555/1‑150 (MQ=255)

   ccgcccgTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTGCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTg                                                                                                                                                    <  1:1585292/150‑1 (MQ=255)

    ggctcgtagtAAGGATCAGTTCGAACTGCTATCCGTGCGTCGGAACACCTTCCACTTAGGGTGAAAGTTGCAGGAGCATCAGCTTACCGTACGGCGGCATAATATGGTAGTTGGTGGCGAGGCAGTGTATGTCTGTGTTCACTGCGGTGa                                                                                                                                                   <  2:738473/146‑1 (MQ=255)

    cgcccgTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGa                                                                                                                                                   <  1:607615/150‑1 (MQ=255)

       ccgTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACa                                                                                                                                                >  2:995005/1‑150 (MQ=255)

        cgtcgtACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAg                                                                                                                                               >  2:1502817/1‑150 (MQ=255)

        cgtcgtACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACCGCAGTGAACac                                                                                                                                               <  2:1557384/150‑2 (MQ=255)

         gtcgtACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACCGCAGTGAACaca                                                                                                                                              <  1:237703/150‑3 (MQ=255)

         gtcgtACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCACTGTGTTCACTGCGGTGAACAGc                                                                                                                                              <  1:67731/150‑1 (MQ=255)

           cgtACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACCGCAGTGAACacagc                                                                                                                                            <  1:512592/150‑5 (MQ=255)

           cgtACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACCGCAGTGAACacagc                                                                                                                                            <  2:469444/150‑5 (MQ=255)

           cgtACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACCGCAGTGAACacagc                                                                                                                                            <  1:258355/150‑5 (MQ=255)

            gtACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTgg                                                                                                                                           >  2:1211989/1‑150 (MQ=255)

              aCGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACCGCAGTGAACacagcgat                                                                                                                                         <  1:262508/150‑8 (MQ=255)

               cGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTg                                                                                                                                        >  1:1313320/1‑150 (MQ=255)

                 cATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCg                                                                                                                                      >  1:583340/1‑150 (MQ=255)

                 cATCAGTTCGAACTGCAATCAGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGAATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCg                                                                                                                                      >  2:565622/1‑150 (MQ=255)

                   tCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGaa                                                                                                                                    <  1:522650/150‑1 (MQ=255)

                    cAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGaaa                                                                                                                                   >  2:654600/1‑150 (MQ=255)

                     aGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAAt                                                                                                                                  >  2:477363/1‑150 (MQ=255)

                         cGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAAcc                                                                                                                              >  2:236971/1‑150 (MQ=255)

                         cGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAAcc                                                                                                                              >  2:1214362/1‑150 (MQ=255)

                          gAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCa                                                                                                                             >  2:1096147/1‑150 (MQ=255)

                           aaCTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCAt                                                                                                                            >  2:1105998/1‑150 (MQ=255)

                             cTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCt                                                                                                                          <  1:1498005/150‑1 (MQ=255)

                              tGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTg                                                                                                                         <  2:790000/150‑1 (MQ=255)

                              tGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTg                                                                                                                         <  2:107289/150‑1 (MQ=255)

                               gCAATCCGTGCGTCTCAACACATTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACAGTACGCCGCCATAATCGCGTAGTTCGTGGAGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGa                                                                                                                        >  2:70167/1‑150 (MQ=255)

                               gCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGa                                                                                                                        >  1:1409776/1‑150 (MQ=255)

                               gCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGa                                                                                                                        >  1:1206183/1‑150 (MQ=255)

                                  aTCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACt                                                                                                                     >  1:607066/1‑150 (MQ=255)

                                      gTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCa                                                                                                                 <  2:1123072/150‑1 (MQ=255)

                                       tGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAg                                                                                                                >  1:1012936/1‑150 (MQ=255)

                                        gCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAgg                                                                                                               <  1:16720/150‑1 (MQ=255)

                                         cGTCGCAACACCTTCCCCTACCGCTGCAGGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGa                                                                                                              >  1:636980/1‑150 (MQ=255)

                                             gCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGAGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACtt                                                                                                          >  2:1018146/1‑150 (MQ=255)

                                               aaCACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTc                                                                                                        >  1:1123913/1‑150 (MQ=255)

                                                acacCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTcc                                                                                                       <  1:1626888/150‑1 (MQ=255)

                                                   ccTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCATCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGa                                                                                                    >  1:1059549/1‑150 (MQ=255)

                                                      tCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAAAGTTCCTGAtag                                                                                                 <  2:1324626/150‑3 (MQ=255)

                                                       ccgcTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCGGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATGAGGAACTTTCCTGATCTg                                                                                                <  2:600029/147‑1 (MQ=255)

                                                       ccccTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTg                                                                                                <  1:174493/150‑1 (MQ=255)

                                                       ccccTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTg                                                                                                >  1:857595/1‑150 (MQ=255)

                                                       ccccTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTg                                                                                                <  1:1502817/150‑1 (MQ=255)

                                                         ccTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGc                                                                                              <  1:1105998/150‑1 (MQ=255)

                                                          cTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCt                                                                                             <  1:840555/150‑1 (MQ=255)

                                                           tACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTg                                                                                            <  1:981783/150‑1 (MQ=255)

                                                           tACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTg                                                                                            >  1:673493/1‑150 (MQ=255)

                                                               gCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTg                                                                                        >  2:902506/1‑150 (MQ=255)

                                                                  gCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCAt                                                                                     >  1:1186601/1‑150 (MQ=255)

                                                                      gTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCa                                                                                 <  1:1214362/150‑1 (MQ=255)

                                                                          cAGGAGCATCAGCTTACCGTACGCCGCCATAATCACGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAAc                                                                             >  2:1354142/1‑150 (MQ=255)

                                                                            ggAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAg                                                                           <  2:857595/150‑1 (MQ=255)

                                                                            ggAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAg                                                                           >  2:918573/1‑150 (MQ=255)

                                                                                 aTCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTc                                                                      <  1:918573/150‑1 (MQ=255)

                                                                                 aTCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCATCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTc                                                                      <  2:1059549/150‑1 (MQ=255)

                                                                                  tCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGATGATTGCATACCAAAACATCTTTCg                                                                     <  2:453254/150‑1 (MQ=255)

                                                                                    aGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCt                                                                   <  2:673493/150‑1 (MQ=255)

                                                                                     gCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTa                                                                  <  1:654600/150‑1 (MQ=255)

                                                                                       ttACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACg                                                                <  1:995005/150‑1 (MQ=255)

                                                                                        tACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGt                                                               >  2:1497171/1‑150 (MQ=255)

                                                                                         aCCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACa                                                                            <  2:346920/136‑1 (MQ=255)

                                                                                         aCCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACa                                                                            >  1:346920/1‑136 (MQ=255)

                                                                                          ccGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTg                                                             >  2:1520680/1‑150 (MQ=255)

                                                                                            gTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCt                                                           >  1:1064866/1‑150 (MQ=255)

                                                                                             tACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTg                                                          >  2:2576/1‑150 (MQ=255)

                                                                                                     cATAATGGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGttt                                                  <  2:107080/150‑1 (MQ=255)

                                                                                                       tAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTa                                                >  2:1571740/1‑150 (MQ=255)

                                                                                                       tAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTa                                                >  2:225745/1‑150 (MQ=255)

                                                                                                         aTCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAac                                              >  1:802793/1‑150 (MQ=255)

                                                                                                             cgTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACgg                                          >  1:1409168/1‑150 (MQ=255)

                                                                                                              gTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGa                                         <  1:1096147/150‑1 (MQ=255)

                                                                                                               tAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAg                                        <  1:670989/150‑1 (MQ=255)

                                                                                                                   tCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAg                                    <  2:1278314/150‑1 (MQ=255)

                                                                                                                        gCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATg                               <  2:1012936/150‑1 (MQ=255)

                                                                                                                              ccGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGt                         >  1:1583973/1‑150 (MQ=255)

                                                                                                                                gTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTa                       <  1:174584/150‑1 (MQ=255)

                                                                                                                                    aTCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTg                   <  2:148811/150‑1 (MQ=255)

                                                                                                                                    aTCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTg                   <  2:1409168/150‑1 (MQ=255)

                                                                                                                                      ccagctgtTCACCGCAGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTc                 >  2:1566209/6‑150 (MQ=255)

                                                                                                                                      cGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTc                 <  1:427449/150‑1 (MQ=255)

                                                                                                                                       gCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCt                <  2:1123913/150‑1 (MQ=255)

                                                                                                                                       cagctgtTCCCCGCAGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCt                >  2:1166159/5‑150 (MQ=255)

                                                                                                                                       cagctgtTCACCGCAGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCt                >  1:1405326/5‑150 (MQ=255)

                                                                                                                                        cTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCTa               <  2:214309/150‑1 (MQ=255)

                                                                                                                                         gctgtTCACCGCAGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCTAt              >  1:1034092/3‑150 (MQ=255)

                                                                                                                                            gtTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCTATtgc           <  2:1605615/150‑1 (MQ=255)

                                                                                                                                            gtTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCTATtgc           <  2:1583973/150‑1 (MQ=255)

                                                                                                                                               cACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCTATtgctgc        >  2:116244/1‑150 (MQ=255)

                                                                                                                                               cACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCTATtgatgc        >  1:1554386/1‑150 (MQ=255)

                                                                                                                                                   gCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATg                               >  2:1083721/1‑123 (MQ=255)

                                                                                                                                                   gCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATg                               <  1:1083721/123‑1 (MQ=255)

                                                                                                                                                     ggTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCTATTGCTGCggtggt  >  2:370258/1‑150 (MQ=255)

TTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACGCCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCTATTGCTGCGGTGGT  >  NC_000913/3090080‑3090378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`