BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,193,582	C→A	6.8%	S240* (TCG→TAG)	yqiK →	protein YqiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,193,582	0	C	A	6.8%	397.1 / 11.3	118	S240* (TCG→TAG)	yqiK	protein YqiK
Reads supporting (aligned to +/- strand): ref base C (50/60); new base A (6/2); total (56/62)
Fisher's exact test for biased strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

Only 100 of 123 total aligned reads displayed.

CTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGAAGCAGAGCAGACACGAATTCTGGC  >  NC_000913/3193433‑3193729

cTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATc                                                                                                                                                     >  2:1425067/1‑150 (MQ=255)

cTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATc                                                                                                                                                     >  2:491272/1‑150 (MQ=255)

  cGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATcgc                                                                                                                                                   <  1:1566278/150‑1 (MQ=255)

   gAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATcgcg                                                                                                                                                  <  2:1131182/150‑1 (MQ=255)

       gAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATAGCGC‑ATcgcgattt                                                                                                                                              <  2:182039/150‑5 (MQ=255)

       gAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAg                                                                                                                                              <  1:377381/150‑1 (MQ=255)

        aaCATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGc                                                                                                                                             <  1:1555780/150‑1 (MQ=255)

          cATTTCAATCCGAACAATGCCTTTGCCGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTg                                                                                                                                           >  1:1025126/1‑150 (MQ=255)

           aTTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTgg                                                                                                                                          >  2:262172/1‑150 (MQ=255)

            tttCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGga                                                                                                                                         <  2:212924/150‑1 (MQ=255)

               cAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGAt                                                                                                                                      >  1:1428366/1‑150 (MQ=255)

               cAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTAGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGAt                                                                                                                                      >  2:383203/1‑150 (MQ=255)

                aaTCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGAtt                                                                                                                                     >  1:929632/1‑150 (MQ=255)

                 aTCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTg                                                                                                                                    <  2:1248004/150‑1 (MQ=255)

                 aTCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTg                                                                                                                                    <  1:1605115/150‑1 (MQ=255)

                  tCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGa                                                                                                                                   >  1:786551/1‑150 (MQ=255)

                   ccGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGaa                                                                                                                                  <  1:691931/150‑1 (MQ=255)

                     taaCAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCCCCGTCGCCGCCAACGTAACGAAGTTGAACAGGATGAAGAAGATGCGGTGCGTGAAAAAAAACGCGAT‑GCGATATAGCGCAAGCTGGACATTGAACa                                                                                                                                >  1:499217/2‑150 (MQ=255)

                     gAACAATGCCTTTGACGCCGAATGTTTAACCAAACTGACTCAGTAAACAGAGCGCCGTGGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCTCGAT‑GATCTATCGCGAAAGCTGGAGATTGaaga                                                                                                                                <  2:692420/150‑3 (MQ=255)

                     gAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATAGCGCAAGCTGGAGATTGAACa                                                                                                                                >  1:499213/1‑150 (MQ=255)

                          aTGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCaag                                                                                                                           <  1:570838/150‑1 (MQ=255)

                           tGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCTGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCaaga                                                                                                                          >  1:765090/1‑150 (MQ=255)

                            gCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCaagaa                                                                                                                         >  2:897388/1‑150 (MQ=255)

                                 tGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGtt                                                                                                                    <  1:778000/150‑1 (MQ=255)

                                       cGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGAc                                                                                                              >  1:1560181/1‑150 (MQ=255)

                                       cGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGAc                                                                                                              >  2:1106073/1‑150 (MQ=255)

                                            gTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCAGCGATCGTAAGGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTg                                                                                                         <  2:1428366/150‑1 (MQ=255)

                                              ttAACCAAACTGACTCAGGAAACAGAGCGCCGACGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTgag                                                                                                       >  2:1149246/1‑150 (MQ=255)

                                                 aCCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTgagcag                                                                                                    >  1:178135/1‑150 (MQ=255)

                                                 aCCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTgagcag                                                                                                    >  1:118611/1‑150 (MQ=255)

                                                  ccAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACg                                                                                                             >  1:551482/1‑140 (MQ=255)

                                                  ccAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACg                                                                                                             <  2:551482/140‑1 (MQ=255)

                                                  ccAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTgagcagg                                                                                                   >  2:283372/1‑150 (MQ=255)

                                                  ccAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTgagcagg                                                                                                   >  1:1403523/1‑150 (MQ=255)

                                                   cAAACTGACTCAGGAAACCGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAACTCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTgagcatga                                                                                                  <  2:899605/150‑1 (MQ=255)

                                                   cAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTgagcagga                                                                                                  <  2:1039851/150‑1 (MQ=255)

                                                   cAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTgagca                                                                                                     >  2:1126399/1‑147 (MQ=255)

                                                   cAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTgagca                                                                                                     <  1:1126399/147‑1 (MQ=255)

                                                     aaCTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTgagcaggagc                                                                                                <  2:756109/150‑1 (MQ=255)

                                                     aaCTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGAGGTAGAAGAAGAGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTgagcaggagc                                                                                                >  1:459884/1‑150 (MQ=255)

                                                       cTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTgagcaggagcag                                                                                              <  1:880207/150‑1 (MQ=255)

                                                         gACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAgcagca                                                                                            >  1:572769/1‑150 (MQ=255)

                                                           cTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAgg                                                                                          >  2:825197/1‑150 (MQ=255)

                                                           cTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAgg                                                                                          <  2:120830/150‑1 (MQ=255)

                                                             cAGGAAACAGAGCGCCGTCGCCGAGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGAGTGAAAAAAATCGCGAT‑GCGCAATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTCAGCAGGAGCAGCAGGtt                                                                                        >  2:1498153/1‑150 (MQ=255)

                                                             cAGGAAACAGAGCGCAGTCGCAGCGAAAGTAACTAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGAGAT‑GCGCTAACGAGCAAGAAGGAGATTGAACAGCAAGAAGCGTATAGAACGCATGAGCATGAGCAGCAGGtt                                                                                        >  2:833808/1‑150 (MQ=255)

                                                              aGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTTAAAAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTa                                                                                       <  1:291706/150‑1 (MQ=255)

                                                                gAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGTAGGAGCAGCAGGTTaaa                                                                                     <  1:922196/150‑1 (MQ=255)

                                                                gAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTaaa                                                                                     <  1:318030/150‑1 (MQ=255)

                                                                   aCAGAGCGCCGTCCCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAAcc                                                                                  <  2:674149/150‑1 (MQ=255)

                                                                    cAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAAccc                                                                                 >  2:1166842/1‑150 (MQ=255)

                                                                     agagCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCg                                                                                >  2:456652/1‑150 (MQ=255)

                                                                     agagCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCg                                                                                <  2:1148238/150‑1 (MQ=255)

                                                                            cGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGcc                                                                         <  1:1425067/150‑1 (MQ=255)

                                                                             gTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCg                                                                        <  2:832897/150‑1 (MQ=255)

                                                                             gTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCg                                                                        <  2:509373/150‑1 (MQ=255)

                                                                                 ccgcGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACa                                                                    <  2:929632/150‑1 (MQ=255)

                                                                                  cgcgAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAg                                                                   >  2:815568/1‑150 (MQ=255)

                                                                                    cgAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGaa                                                                 <  2:1403523/150‑1 (MQ=255)

                                                                                      aaCGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATg                                                               <  1:945488/150‑1 (MQ=255)

                                                                                       aCGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGc                                                              <  2:76416/150‑1 (MQ=255)

                                                                                         gTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCa                                                             <  2:604618/149‑1 (MQ=255)

                                                                                         gTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCa                                                             >  1:604618/1‑149 (MQ=255)

                                                                                          tAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACTCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTg                                                                           <  2:207909/134‑1 (MQ=255)

                                                                                          tAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTg                                                                           >  1:207909/1‑134 (MQ=255)

                                                                                          tAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAg                                                                   >  2:117172/1‑142 (MQ=255)

                                                                                          tAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAg                                                                   <  1:117172/142‑1 (MQ=255)

                                                                                          tAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACg                                                           <  1:1106073/150‑1 (MQ=255)

                                                                                            aCGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTa                                                         >  1:278698/1‑150 (MQ=255)

                                                                                               aaGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTg                                                      >  1:387684/1‑150 (MQ=255)

                                                                                                aGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGc                                                     <  1:1070758/150‑1 (MQ=255)

                                                                                                 gTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCg                                                    <  1:797065/150‑1 (MQ=255)

                                                                                                      aCAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCttt                                               <  1:491272/150‑1 (MQ=255)

                                                                                                       cAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCtttt                                              <  1:678797/150‑1 (MQ=255)

                                                                                                        aGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTg                                             <  1:385174/150‑1 (MQ=255)

                                                                                                           aTGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAg                                          <  1:267278/150‑1 (MQ=255)

                                                                                                           aTGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATAGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAg                                          <  2:499213/150‑1 (MQ=255)

                                                                                                            tGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGc                                         <  2:572769/150‑1 (MQ=255)

                                                                                                             gTAGAAGTTCCGGTGAGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCt                                        >  2:891985/1‑150 (MQ=255)

                                                                                                                 aaGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAAc                                    <  2:765090/150‑1 (MQ=255)

                                                                                                                     tGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAAcgtcg                                >  2:265274/1‑150 (MQ=255)

                                                                                                                      gCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAAcgtcgt                               >  1:1608175/1‑150 (MQ=255)

                                                                                                                        ggTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGGGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAAcgtcgtcg                             >  1:983/1‑150 (MQ=255)

                                                                                                                            cGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAAcgtcgtcgt                            >  2:1124574/1‑147 (MQ=255)

                                                                                                                            cGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGaa                         >  2:1531466/1‑150 (MQ=255)

                                                                                                                            cGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGaa                         >  2:1525733/1‑150 (MQ=255)

                                                                                                                             gTGAAAAAAATGGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTT‑AAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGAagc                       <  2:1249712/150‑1 (MQ=255)

                                                                                                                             gTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCTGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGAag                        <  2:1560181/150‑1 (MQ=255)

                                                                                                                              tGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGAagc                       >  1:1212882/1‑150 (MQ=255)

                                                                                                                                aaaaaaaTCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGAagcag                     <  1:1507684/150‑1 (MQ=255)

                                                                                                                                   aaaaTCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGAagcagagc                  <  2:178135/150‑1 (MQ=255)

                                                                                                                                       ccagctt‑GCGCGATAGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGAAgcagagcagaa              >  2:1388139/7‑149 (MQ=255)

                                                                                                                                        cgcgAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGAAGCAGAGCAGaca             <  2:1239114/150‑1 (MQ=255)

                                                                                                                                         gcgAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGAAGCAGAGCAGacac            >  1:713089/1‑150 (MQ=255)

                                                                                                                                         agctt‑GCGCGATAGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGAAGCAGAGCAGacac            >  2:438808/5‑150 (MQ=255)

                                                                                                                                         agctt‑GCGCGATAGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGAAGCAGAGCAGacac            >  2:152214/5‑150 (MQ=255)

                                                                                                                                         agatt‑GCGCGATAGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGAAGCAGAGCAGacac            >  1:665798/5‑150 (MQ=255)

                                                                                                                                          cgAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGAAGCAGAGCAGACACg           >  1:1015430/1‑150 (MQ=255)

                                                                                                                                                   tATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGAAGCAGAGCAGACACGAATTCTgg   >  1:1021210/1‑150 (MQ=255)

                                                                                                                                                    aTCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGAAGCAGAGCAGACACGAATTCTGGc  >  2:171904/1‑150 (MQ=255)

CTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAT‑GCGCTATCGCGCAAGCTGGAGATTGAACAGCAAGAAGCGTTTATGACGCTTGAGCAGGAGCAGCAGGTTAAAACCCGTACTGCCGAACAGAATGCACGTATTGCGGCTTTTGAAGCTGAACGTCGTCGTGAAGCAGAGCAGACACGAATTCTGGC  >  NC_000913/3193433‑3193729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`