Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,241,443 A→G 6.5% I101T (ATC→ACC)  ygjV ← inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,241,4430AG 6.5% 233.8 / 5.2 77I101T (ATC→ACC) ygjVinner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (38/34);  new base G (3/2);  total (41/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

TAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCG  >  NC_000913/3241294‑3241589
                                                                                                                                                     |                                                                                                                                                  
tAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGTGCCCAGGTACTGACAATCGTGCCGa                                                                                                                                                    >  1:827803/1‑150 (MQ=255)
  aGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATa                                                                                                                                                  >  1:1077324/1‑150 (MQ=255)
  aGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATa                                                                                                                                                  <  2:911226/150‑1 (MQ=255)
  aGCACAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATa                                                                                                                                                  <  2:1168061/150‑1 (MQ=255)
    cAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAAc                                                                                                                                                <  2:1429655/150‑1 (MQ=255)
     aGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAAcc                                                                                                                                               <  2:1082962/150‑1 (MQ=255)
        aaaCTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGGCCTTTACAGCAGAATAGCGCCCAGGTACTGAGAATCGTGCCGATAACCGGc                                                                                                                                            <  2:615529/150‑1 (MQ=255)
        aaaCTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGc                                                                                                                                            <  1:181828/150‑1 (MQ=255)
         aaCTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCa                                                                                                                                           <  2:399465/150‑1 (MQ=255)
              cccTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGGTTATCGGCacgatt                                                                                                                                      <  1:1527443/150‑6 (MQ=255)
                      catcGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAgg                                                                                                                              >  1:852005/1‑150 (MQ=255)
                        tcGTGCCGCCTCTCGACCCCGCCCAGAAGTTGAGAAGCACCCAGCAACACGTTGAAAACCACCCTACGCAGCGCATGGTCCGCCCTTTACAGCACAATAGCGCCCAGGTACTGACAATCGGGCCGATAACCGGCAATAGTGCGACAGcat                                                                                                                            <  2:1478807/150‑1 (MQ=255)
                          gTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGgatga                                                                                                                          >  1:626160/1‑150 (MQ=255)
                            gccgccTATCGCCCCCGCCCAGAAGTTGTGAATCCCCCAGCAACACGTTGAAAACCACATTACGCCGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACCATCGTGCCGATACCCGGCAATAGTTCGACAGGatgatg                                                                                                                        >  2:840894/1‑150 (MQ=255)
                                  tATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACtt                                                                                                                  >  2:767772/1‑150 (MQ=255)
                                    tCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTcg                                                                                                                <  2:835153/150‑1 (MQ=255)
                                      gACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTcgcg                                                                                                              <  1:120380/150‑1 (MQ=255)
                                       aCCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAGCCGGCAATAGTTCGACAGGATGATGGAACTTCGCGa                                                                                                             >  2:926744/1‑150 (MQ=255)
                                          ccGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGc                                                                                                          <  1:853687/150‑1 (MQ=255)
                                          ccGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGc                                                                                                          >  1:383653/1‑150 (MQ=255)
                                          ccGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGc                                                                                                          <  1:1228997/150‑1 (MQ=255)
                                             cccAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCaa                                                                                                       >  2:1055144/1‑150 (MQ=255)
                                                aGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTc                                                                                                    <  2:626160/150‑1 (MQ=255)
                                                   aGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGc                                                                                                 <  1:1301528/150‑1 (MQ=255)
                                                    gTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGcc                                                                                                <  1:767772/150‑1 (MQ=255)
                                                     ttGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCa                                                                                               <  2:738722/150‑1 (MQ=255)
                                                       gtgAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCATTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATACCCGGCAATAGTTCGACAGGATGATGGACCTTCGCGAGGCCAATTCCGCCAGt                                                                                             >  2:1053932/1‑150 (MQ=255)
                                                              aCCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGcaca                                                                                      >  2:1192110/1‑150 (MQ=255)
                                                                 cAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATa                                                                                   >  2:1430892/1‑150 (MQ=255)
                                                                  aGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATaa                                                                                  <  1:1248627/150‑1 (MQ=255)
                                                                   gCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATaaa                                                                                 >  1:879564/1‑150 (MQ=255)
                                                                     aaCACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATaaaaa                                                                               >  2:1383747/1‑150 (MQ=255)
                                                                     aaCACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATaaaaa                                                                               <  2:843845/150‑1 (MQ=255)
                                                                     aaCACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCG‑‑CAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATc                                                                             <  2:552246/150‑1 (MQ=255)
                                                                      acacGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAAt                                                                              >  2:1462855/1‑150 (MQ=255)
                                                                         cattGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGAATTAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGc                                                                           >  2:711021/3‑150 (MQ=255)
                                                                         cGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGc                                                                           >  1:125418/1‑150 (MQ=255)
                                                                         cGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGc                                                                           >  1:1433502/1‑150 (MQ=255)
                                                                          gTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGcc                                                                          <  1:23762/150‑1 (MQ=255)
                                                                           ttGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCa                                                                         >  2:711764/1‑150 (MQ=255)
                                                                            tGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCAt                                                                        >  1:714150/1‑150 (MQ=255)
                                                                             gAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCAtt                                                                       <  1:935586/150‑1 (MQ=255)
                                                                               aaaCCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTAc                                                                     >  1:1173483/1‑150 (MQ=255)
                                                                                aaCCACATTACGCAGCTCATGGTCAGCCCTTTACAGCAGAATAGTGCTCTGGTTCTTAGAATCTTGCCGATAACCGGCAATAGTTCGACAGGATGATGTAACTTCGCGAGGCCAATTCCGACAGTCAGCTCAATAAAAATCGCCATTAcc                                                                    <  2:827803/150‑1 (MQ=255)
                                                                                  ccACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCa                                                                  >  2:85364/1‑150 (MQ=255)
                                                                                    acaTTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATa                                                                <  1:780410/150‑1 (MQ=255)
                                                                                         aCGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTg                                                           >  1:1621957/1‑150 (MQ=255)
                                                                                           gCAGCGCATGGTCAGCCATTTACAGCATAATAGCTCCCAGGTACTGACAATCGTGCCGATAACCGGAAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTgcg                                                         <  1:864912/150‑1 (MQ=255)
                                                                                           gCAGCGCATGGTCAGCACTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTgcg                                                         >  2:401524/1‑150 (MQ=255)
                                                                                              gcgcATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCgcgt                                                      >  1:378044/1‑150 (MQ=255)
                                                                                                   tGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTa                                                 <  2:383653/150‑1 (MQ=255)
                                                                                                    ggTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTaa                                                <  1:1430892/150‑1 (MQ=255)
                                                                                                       cAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGt                                             <  1:1462855/150‑1 (MQ=255)
                                                                                                           ccTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTTCGTAAGGTAATc                                         <  1:401524/150‑1 (MQ=255)
                                                                                                           acTTTACAGCAGAATAGGGCCCATGTACTGAGAATCGTGCCGATTACAGGATATAGTTCGACAGGTTGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACACATAAGCTGCGCGTGCGTAAGGTAATc                                         <  1:1198124/149‑1 (MQ=255)
                                                                                                               tACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATg                                     <  2:1077324/150‑1 (MQ=255)
                                                                                                                   gcagAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACg                                 >  2:1378623/1‑150 (MQ=255)
                                                                                                                   gcagAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACg                                 >  2:1291841/1‑150 (MQ=255)
                                                                                                                   gcagAATAGCGCCCAGGTACTGACAATCGTGCCGATAACAGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGCCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTAACCATAAGATGCGAGAGCGTAAGGTAATCAATGTACg                                 >  2:1528115/1‑150 (MQ=255)
                                                                                                                        aTAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATgg                            <  1:1055144/150‑1 (MQ=255)
                                                                                                                          aGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCa                          >  2:331950/1‑150 (MQ=255)
                                                                                                                          aGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCa                          >  2:325884/1‑150 (MQ=255)
                                                                                                                           gcgcCCAGGTACTGACAATCGTGCCGATAACCGGAAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCAt                         >  2:305324/1‑150 (MQ=255)
                                                                                                                              cccAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTaa                      >  2:1492174/1‑150 (MQ=255)
                                                                                                                               ccAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAg                     <  1:242204/150‑1 (MQ=255)
                                                                                                                                  ggTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCATGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAt                  >  2:352973/1‑150 (MQ=255)
                                                                                                                                    tACTGACAATCGTGCCGATAACCGGAAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtgg                <  1:711021/150‑1 (MQ=255)
                                                                                                                                        gACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcact            >  2:1309925/1‑150 (MQ=255)
                                                                                                                                        gACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcact            >  2:1412849/1‑150 (MQ=255)
                                                                                                                                         gaactattGCCGGTTATCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactg           >  2:5821/8‑150 (MQ=255)
                                                                                                                                          cAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactgg          >  1:768632/1‑150 (MQ=255)
                                                                                                                                          cAATCGTACCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTACACCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactgg          >  2:410515/1‑150 (MQ=255)
                                                                                                                                           aaTCGTGCCGATAACCGGCAATAGTTCGACAGGATTATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactggc         <  1:1381781/150‑1 (MQ=255)
                                                                                                                                            ctattGCCGGTTATCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAAt                              <  2:1393419/124‑1 (MQ=255)
                                                                                                                                            ctattGCCGGTTATCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAAt                              >  1:1393419/5‑128 (MQ=255)
                                                                                                                                             tattGCCGGTTATCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactggcac       >  2:1313502/4‑150 (MQ=255)
                                                                                                                                                tGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGAGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAg    >  1:779481/1‑150 (MQ=255)
                                                                                                                                                  ccGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCg  >  1:931301/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TAAGCAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCG  >  NC_000913/3241294‑3241589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑