Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,241,447 C→T 6.7% V100I (GTT→ATT) ‡ ygjV ← inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,241,4470CT 6.7% 227.1 / 4.5 74V100I (GTT→ATT) ‡ygjVinner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (39/30);  new base T (3/2);  total (42/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

CAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGG  >  NC_000913/3241298‑3241596
                                                                                                                                                     |                                                                                                                                                     
cAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAAc                                                                                                                                                       <  2:1429655/150‑1 (MQ=255)
 aGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAAcc                                                                                                                                                      <  2:1082962/150‑1 (MQ=255)
    aaaCTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGGCCTTTACAGCAGAATAGCGCCCAGGTACTGAGAATCGTGCCGATAACCGGc                                                                                                                                                   <  2:615529/150‑1 (MQ=255)
    aaaCTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGc                                                                                                                                                   <  1:181828/150‑1 (MQ=255)
     aaCTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCa                                                                                                                                                  <  2:399465/150‑1 (MQ=255)
          cccTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGGTTATCGGCacgatt                                                                                                                                             <  1:1527443/150‑6 (MQ=255)
                  catcGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAgg                                                                                                                                     >  1:852005/1‑150 (MQ=255)
                    tcGTGCCGCCTCTCGACCCCGCCCAGAAGTTGAGAAGCACCCAGCAACACGTTGAAAACCACCCTACGCAGCGCATGGTCCGCCCTTTACAGCACAATAGCGCCCAGGTACTGACAATCGGGCCGATAACCGGCAATAGTGCGACAGcat                                                                                                                                   <  2:1478807/150‑1 (MQ=255)
                      gTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGgatga                                                                                                                                 >  1:626160/1‑150 (MQ=255)
                        gccgccTATCGCCCCCGCCCAGAAGTTGTGAATCCCCCAGCAACACGTTGAAAACCACATTACGCCGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACCATCGTGCCGATACCCGGCAATAGTTCGACAGGatgatg                                                                                                                               >  2:840894/1‑150 (MQ=255)
                              tATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACtt                                                                                                                         >  2:767772/1‑150 (MQ=255)
                                tCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTcg                                                                                                                       <  2:835153/150‑1 (MQ=255)
                                  gACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTcgcg                                                                                                                     <  1:120380/150‑1 (MQ=255)
                                   aCCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAGCCGGCAATAGTTCGACAGGATGATGGAACTTCGCGa                                                                                                                    >  2:926744/1‑150 (MQ=255)
                                      ccGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGc                                                                                                                 <  1:853687/150‑1 (MQ=255)
                                      ccGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGc                                                                                                                 <  1:1228997/150‑1 (MQ=255)
                                      ccGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGc                                                                                                                 >  1:383653/1‑150 (MQ=255)
                                         cccAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCaa                                                                                                              >  2:1055144/1‑150 (MQ=255)
                                            aGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTc                                                                                                           <  2:626160/150‑1 (MQ=255)
                                               aGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGc                                                                                                        <  1:1301528/150‑1 (MQ=255)
                                                gTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGcc                                                                                                       <  1:767772/150‑1 (MQ=255)
                                                 ttGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCa                                                                                                      <  2:738722/150‑1 (MQ=255)
                                                   gtgAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCATTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATACCCGGCAATAGTTCGACAGGATGATGGACCTTCGCGAGGCCAATTCCGCCAGt                                                                                                    >  2:1053932/1‑150 (MQ=255)
                                                          aCCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGcaca                                                                                             >  2:1192110/1‑150 (MQ=255)
                                                             cAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATa                                                                                          >  2:1430892/1‑150 (MQ=255)
                                                              aGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATaa                                                                                         <  1:1248627/150‑1 (MQ=255)
                                                               gCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATaaa                                                                                        >  1:879564/1‑150 (MQ=255)
                                                                 aaCACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATaaaaa                                                                                      <  2:843845/150‑1 (MQ=255)
                                                                 aaCACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATaaaaa                                                                                      >  2:1383747/1‑150 (MQ=255)
                                                                 aaCACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCG‑‑CAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATc                                                                                    <  2:552246/150‑1 (MQ=255)
                                                                  acacGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAAt                                                                                     >  2:1462855/1‑150 (MQ=255)
                                                                     cattGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGAATTAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGc                                                                                  >  2:711021/3‑150 (MQ=255)
                                                                     cGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGc                                                                                  >  1:125418/1‑150 (MQ=255)
                                                                     cGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGc                                                                                  >  1:1433502/1‑150 (MQ=255)
                                                                      gTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGcc                                                                                 <  1:23762/150‑1 (MQ=255)
                                                                       ttGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCa                                                                                >  2:711764/1‑150 (MQ=255)
                                                                        tGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCAt                                                                               >  1:714150/1‑150 (MQ=255)
                                                                         gAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCAtt                                                                              <  1:935586/150‑1 (MQ=255)
                                                                           aaaCCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTAc                                                                            >  1:1173483/1‑150 (MQ=255)
                                                                            aaCCACATTACGCAGCTCATGGTCAGCCCTTTACAGCAGAATAGTGCTCTGGTTCTTAGAATCTTGCCGATAACCGGCAATAGTTCGACAGGATGATGTAACTTCGCGAGGCCAATTCCGACAGTCAGCTCAATAAAAATCGCCATTAcc                                                                           <  2:827803/150‑1 (MQ=255)
                                                                              ccACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCa                                                                         >  2:85364/1‑150 (MQ=255)
                                                                                acaTTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATa                                                                       <  1:780410/150‑1 (MQ=255)
                                                                                     aCGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTg                                                                  >  1:1621957/1‑150 (MQ=255)
                                                                                       gCAGCGCATGGTCAGCCATTTACAGCATAATAGCTCCCAGGTACTGACAATCGTGCCGATAACCGGAAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTgcg                                                                <  1:864912/150‑1 (MQ=255)
                                                                                       gCAGCGCATGGTCAGCACTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTgcg                                                                >  2:401524/1‑150 (MQ=255)
                                                                                          gcgcATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCgcgt                                                             >  1:378044/1‑150 (MQ=255)
                                                                                               tGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTa                                                        <  2:383653/150‑1 (MQ=255)
                                                                                                ggTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTaa                                                       <  1:1430892/150‑1 (MQ=255)
                                                                                                   cAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGt                                                    <  1:1462855/150‑1 (MQ=255)
                                                                                                       ccTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTTCGTAAGGTAATc                                                <  1:401524/150‑1 (MQ=255)
                                                                                                       acTTTACAGCAGAATAGGGCCCATGTACTGAGAATCGTGCCGATTACAGGATATAGTTCGACAGGTTGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACACATAAGCTGCGCGTGCGTAAGGTAATc                                                <  1:1198124/149‑1 (MQ=255)
                                                                                                           tACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATg                                            <  2:1077324/150‑1 (MQ=255)
                                                                                                               gcagAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACg                                        >  2:1378623/1‑150 (MQ=255)
                                                                                                               gcagAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACg                                        >  2:1291841/1‑150 (MQ=255)
                                                                                                               gcagAATAGCGCCCAGGTACTGACAATCGTGCCGATAACAGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGCCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTAACCATAAGATGCGAGAGCGTAAGGTAATCAATGTACg                                        >  2:1528115/1‑150 (MQ=255)
                                                                                                                    aTAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATgg                                   <  1:1055144/150‑1 (MQ=255)
                                                                                                                      aGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCa                                 >  2:325884/1‑150 (MQ=255)
                                                                                                                      aGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCa                                 >  2:331950/1‑150 (MQ=255)
                                                                                                                       gcgcCCAGGTACTGACAATCGTGCCGATAACCGGAAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCAt                                >  2:305324/1‑150 (MQ=255)
                                                                                                                          cccAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTaa                             >  2:1492174/1‑150 (MQ=255)
                                                                                                                           ccAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAg                            <  1:242204/150‑1 (MQ=255)
                                                                                                                              ggTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCATGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAt                         >  2:352973/1‑150 (MQ=255)
                                                                                                                                tACTGACAATCGTGCCGATAACCGGAAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtgg                       <  1:711021/150‑1 (MQ=255)
                                                                                                                                    gACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcact                   >  2:1309925/1‑150 (MQ=255)
                                                                                                                                    gACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcact                   >  2:1412849/1‑150 (MQ=255)
                                                                                                                                     gaactattGCCGGTTATCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactg                  >  2:5821/8‑150 (MQ=255)
                                                                                                                                      cAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactgg                 >  1:768632/1‑150 (MQ=255)
                                                                                                                                      cAATCGTACCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTACACCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactgg                 >  2:410515/1‑150 (MQ=255)
                                                                                                                                       aaTCGTGCCGATAACCGGCAATAGTTCGACAGGATTATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactggc                <  1:1381781/150‑1 (MQ=255)
                                                                                                                                        ctattGCCGGTTATCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAAt                                     >  1:1393419/5‑128 (MQ=255)
                                                                                                                                        ctattGCCGGTTATCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAAt                                     <  2:1393419/124‑1 (MQ=255)
                                                                                                                                         tattGCCGGTTATCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGAtggcactggcac              >  2:1313502/4‑150 (MQ=255)
                                                                                                                                            tGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGAGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAg           >  1:779481/1‑150 (MQ=255)
                                                                                                                                              ccGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCg         >  1:931301/1‑150 (MQ=255)
                                                                                                                                                    aCCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAg   >  1:127446/1‑150 (MQ=255)
                                                                                                                                                    aCCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAg   >  1:971780/1‑150 (MQ=255)
                                                                                                                                                     ccGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAgg  <  1:840894/150‑1 (MQ=255)
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CAGAAAACTACCCTCAATCATCGTGCCGCCTATCGACCCCGCCCAGAAGTTGTGAATCACCCAGCAACACGTTGAAAACCACATTACGCAGCGCATGGTCAGCCCTTTACAGCAGAATAGCGCCCAGGTACTGACAATCGTGCCGATAACCGGCAATAGTTCGACAGGATGATGGAACTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGG  >  NC_000913/3241298‑3241596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑