Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,263,500	G→A	13.7%	P54L (CCG→CTG) ‡	tdcD ←	propionate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,263,500	0	G	A	13.7%	179.2 / 18.0	73	P54L (CCG→CTG) ‡	tdcD	propionate kinase
Reads supporting (aligned to +/- strand):  ref base G (30/33);  new base A (5/5);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.31e-02

CTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGA  >  NC_000913/3263351‑3263649                                                                                                                                                       |                                                                                                                                                      cTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCg                                                                                                                                                       >  1:78574/1‑150 (MQ=255)      tCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCt                                                                                                                                                  <  2:1470828/150‑1 (MQ=255)      tCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCt                                                                                                                                                  <  1:417076/150‑1 (MQ=255)         gTAATAATGGCGGACTCGGTAAAACTACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGc                                                                                                                                               <  2:624576/150‑1 (MQ=255)             taatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCACCGCTGGctcac                                                                                                                                           <  1:1463128/150‑3 (MQ=255)             taatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCACCGCTGGctcac                                                                                                                                           <  1:1470194/150‑3 (MQ=255)             taatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCGTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCACCGCTGGctcac                                                                                                                                           <  1:515103/150‑3 (MQ=255)              aatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcc                                                                                                                                          >  2:182082/1‑150 (MQ=255)               atGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTccc                                                                                                                                         <  2:471526/150‑1 (MQ=255)                tGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcccc                                                                                                                                        <  1:281688/150‑1 (MQ=255)                  gCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTAAATGCACCTTCGTAGCTGTGGTGAGCCAGCACCGCTGGCTCAccacag                                                                                                                                      <  2:297645/150‑5 (MQ=255)                   cGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAt                                                                                                                                     >  2:1373865/1‑150 (MQ=255)                         cGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACgg                                                                                                                               >  2:952543/1‑150 (MQ=255)                            tAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATa                                                                                                                            <  2:362013/150‑1 (MQ=255)                              aaatTATTGCCGTCTTGATCGATGCTGTGGCCATTTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAg                                                                                                                          <  1:705782/150‑1 (MQ=255)                                       ccgccgTGAGCGATGCGGTGTCCAATTAAGTCCACAAAGTCATTTAAATTCCGTTTTTCCACTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCAttt                                                                                                                 >  2:565150/1‑150 (MQ=255)                                       ccgccgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCAttt                                                                                                                 >  2:1384500/1‑150 (MQ=255)                                             tGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAg                                                                                                           <  2:239775/150‑1 (MQ=255)                                             tGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAg                                                                                                           <  2:243438/150‑1 (MQ=255)                                                          ggCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCgg                                                                                              <  2:355759/150‑1 (MQ=255)                                                          ggCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTAAGGATAAGAATGCCTTTTACGAGTTAATACCGTCgg                                                                                              >  2:1275808/1‑150 (MQ=255)                                                           gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGc                                                                                             <  2:594975/150‑1 (MQ=255)                                                           gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGc                                                                                             >  2:90129/1‑150 (MQ=255)                                                            ccAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTAAGGATAAGAATGCATTTTACGAGTTAATAACGTCGGCa                                                                                            >  2:372496/1‑150 (MQ=255)                                                                   aGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTg                                                                                     >  2:274104/1‑150 (MQ=255)                                                                    ggCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGa                                                                                    >  2:471698/1‑150 (MQ=255)                                                                    ggCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGa                                                                                    >  2:291476/1‑150 (MQ=255)                                                                     gCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGAc                                                                                   <  1:182082/150‑1 (MQ=255)                                                                      ccACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGAc                                                                                   <  1:575453/150‑1 (MQ=255)                                                                        acacTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACAtt                                                                                <  2:726998/150‑1 (MQ=255)                                                                           cTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTTGCATTTAt                                                                             >  1:1227432/1‑150 (MQ=255)                                                                               cATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACtt                                                                         >  1:534317/1‑150 (MQ=255)                                                                                  ttAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTcac                                                                      <  1:1468361/150‑1 (MQ=255)                                                                                          cGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTg                                                              >  1:530855/1‑150 (MQ=255)                                                                                          cGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTg                                                              <  2:527476/150‑1 (MQ=255)                                                                                             ttttCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGCGCCCGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTACGAGGTAATACCGTCGGCAATACCTGACATTAATACTTGACAGTCGCTGGcc                                                           >  1:1495843/1‑149 (MQ=255)                                                                                              tttCCAGTTC‑AAATGCAATTGCCCTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGACAt                                                          <  2:191112/150‑1 (MQ=255)                                                                                                tgcAGTTC‑AAATGCAATTGCCTTCACTGCACCTTCGGAGCTGTGGTGCGCCCCCGGCGGTGGCTGTCCCGCGTTTGAGGGTAAGAATACATTTTGCGAGGTGATGCCCTAGGCAAAACATGACCTGAATAGTTGCCACTAGCTTGCAtag                                                        >  2:1266663/3‑148 (MQ=255)                                                                                                tCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCg                                                        >  2:1017818/1‑150 (MQ=255)                                                                                                tCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCg                                                        <  2:49014/150‑1 (MQ=255)                                                                                                  cAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAg                                                      >  2:1195063/1‑150 (MQ=255)                                                                                                   aGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGc                                                     <  1:1601906/150‑1 (MQ=255)                                                                                                    gTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCa                                                    <  1:1264699/150‑1 (MQ=255)                                                                                                       c‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACgg                                                 <  2:404723/150‑1 (MQ=255)                                                                                                       c‑AAATGCAATTGCCTTCAATGCAACTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTACGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACgg                                                 >  2:1265649/1‑150 (MQ=255)                                                                                                          aaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCACCGGTTCAGGCTATACCCCAATTTAGGATAATAATTCATTTTACGAGTTTATAACGTAGGCAAAAACAGAAAATAATAATTGAAAGTAGCTGGCATAGAGCACCGaa                                               >  2:1467718/1‑150 (MQ=255)                                                                                                                   gCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATc                                      >  2:1128549/1‑150 (MQ=255)                                                                                                                    ccTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCg                                     <  1:609154/150‑1 (MQ=255)                                                                                                                    ccTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCg                                     <  2:250894/150‑1 (MQ=255)                                                                                                                     cTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGa                                    >  1:65946/1‑150 (MQ=255)                                                                                                                     cTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGa                                    <  1:1371606/150‑1 (MQ=255)                                                                                                                        cAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTAACAGTAGCTGGCATCGAGCACGGAAAACTTAATCGAAGa                                 >  2:1199405/1‑150 (MQ=255)                                                                                                                         aaTGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGAc                                >  1:499786/1‑150 (MQ=255)                                                                                                                         aaTGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTACCCCAATTAAGGATAAGAATGCATTTTACGAGTTAATACCGTCGGCAATAACTGACAATAATACTTCACAGTCGCTGGCATCGAGCACGGAAAAAATAATCGAAGAc                                >  2:858291/1‑150 (MQ=255)                                                                                                                          aTGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACg                               <  2:499786/150‑1 (MQ=255)                                                                                                                             cACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAAc                            >  1:1321903/1‑150 (MQ=255)                                                                                                                              aCCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAAcc                           <  1:1467718/150‑1 (MQ=255)                                                                                                                               ccTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACAATAATAATTCACAGTCGCTGGCATAGAGCAAGGAAAACTTAATAGAAGACGAACca                          >  2:1177561/1‑150 (MQ=255)                                                                                                                                    gTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGtt                     <  1:89965/150‑1 (MQ=255)                                                                                                                                     tAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTta                    <  1:1384500/150‑1 (MQ=255)                                                                                                                                           tggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAAcc              <  1:90129/150‑1 (MQ=255)                                                                                                                                           gggagAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAAcc              >  1:994607/5‑150 (MQ=255)                                                                                                                                           gggagAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAAc               >  2:321867/5‑149 (MQ=255)                                                                                                                                           gggagAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAAc               <  1:321867/145‑1 (MQ=255)                                                                                                                                            ggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCa             >  2:630573/1‑150 (MQ=255)                                                                                                                                            ggagAGCCAGCACCGCTGGCTCACCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCa             >  1:552445/4‑150 (MQ=255)                                                                                                                                             gagAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaa            >  2:302675/3‑150 (MQ=255)                                                                                                                                             gagAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaa            >  2:1225276/3‑150 (MQ=255)                                                                                                                                              tgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaaa           <  1:171201/150‑1 (MQ=255)                                                                                                                                                aGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaac         <  1:565150/150‑1 (MQ=255)                                                                                                                                                 gCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaaca        >  1:1124474/1‑150 (MQ=255)                                                                                                                                                 gCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaaca        >  1:461236/1‑150 (MQ=255)                                                                                                                                                     gCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCg    <  1:291476/150‑1 (MQ=255)                                                                                                                                                      cGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATAACTTACATTAATACTTCACAGTCGCTGTCATCGAGCACGGAAAACTTAATCGAAGACTAACCACAGTTAATAACCAAAACAACCgg   <  1:162133/150‑1 (MQ=255)                                                                                                                                                       ggTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGa  >  2:13290/1‑150 (MQ=255)                                                                                                                                                       ggTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATAATTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGa  >  1:684323/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                      CTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTC‑AAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGA  >  NC_000913/3263351‑3263649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑