Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 399,801 A→G 8.0% Q70R (CAA→CGA)  yaiZ → DUF2754 domain‑containing protein YaiZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913399,8010AG 8.0% 325.9 / 14.3 112Q70R (CAA→CGA) yaiZDUF2754 domain‑containing protein YaiZ
Reads supporting (aligned to +/- strand):  ref base A (46/57);  new base G (2/7);  total (48/64)
Fisher's exact test for biased strand distribution p-value = 2.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 112 total aligned reads displayed.

ATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATAC  >  NC_000913/399655‑399947
                                                                                                                                                  |                                                                                                                                                  
attcattcTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATa                                                                                                                                                 >  1:1286398/1‑150 (MQ=255)
attcattcTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGGCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATa                                                                                                                                                 <  2:784669/150‑1 (MQ=255)
 ttcattcTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATaa                                                                                                                                                >  1:1085884/1‑150 (MQ=255)
  tcattcTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGGTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAg                                                                                                                                               <  2:727770/150‑1 (MQ=255)
    attcTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGaa                                                                                                                                             <  2:508101/150‑1 (MQ=255)
    attcTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGaa                                                                                                                                             <  1:1298053/150‑1 (MQ=255)
     ttcTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAat                                                                                                                                            >  2:1086710/1‑150 (MQ=255)
      tcTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAata                                                                                                                                           <  2:1286398/150‑1 (MQ=255)
       cTTAATGGCGTGGTGGGGTTACTGGGAGTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTTGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAATCGCCCAATAAGAatat                                                                                                                                          <  1:861756/150‑1 (MQ=255)
            tGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCg                                                                                                                                     >  1:585372/1‑150 (MQ=255)
             ggCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCgg                                                                                                                                    >  1:467369/1‑150 (MQ=255)
             ggCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCgg                                                                                                                                    <  1:486276/150‑1 (MQ=255)
             ggCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCgg                                                                                                                                    >  2:901408/1‑150 (MQ=255)
                gtggtgGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCg                                                                                                                                 <  2:260389/150‑1 (MQ=255)
                 tggtgtGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGTTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCGATAATATTCTTATTGGGCGt                                                                                                                                <  1:548494/150‑1 (MQ=255)
                 gggtggGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCGATAATATTCTTATTGGGCGt                                                                                                                                <  1:543072/149‑1 (MQ=255)
                  ggtggGGTTACTGGGACTTGAAGCAAAAGGCGGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGtt                                                                                                                               <  2:306942/150‑1 (MQ=255)
                   gtggGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTa                                                                                                                              <  1:1380598/150‑1 (MQ=255)
                   gtggGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTa                                                                                                                              >  2:1195812/1‑150 (MQ=255)
                     ggggTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCGATAATATTCTTATTGGGCGttttc                                                                                                                            <  2:104905/150‑4 (MQ=255)
                     ggggTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCGATAATATTCTTATTGGGCGttttc                                                                                                                            <  1:1050582/150‑4 (MQ=255)
                     ggggTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTaaa                                                                                                                            >  2:546435/1‑150 (MQ=255)
                      gggTTACTGGGATTTGAAGCAAAAGGTTCGCAGACCTATGCCGTCGGTCTGGGGACGTTGGTGATTAGTTTCTGGCTGTATTGTTTGATTTTTTTTGGTCTCGATGAAGAAATTGAAAATGCCCGATAATATTCTTATTGGTCGttttca                                                                                                                           <  2:870647/150‑5 (MQ=255)
                          tACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATAtt                                                                                                                       <  2:387586/150‑1 (MQ=255)
                            cTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTAc                                                                                                                     <  2:351797/150‑1 (MQ=255)
                            cTGGAAATTGAAGCAAAATGATAGAAGACATATGCCGACGTAAACGTGAACTGCGTGATAATTTTCAGACTGGCCGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTAc                                                                                                                     >  2:918454/1‑150 (MQ=255)
                                gATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATtg                                                                                                                 <  2:161029/150‑1 (MQ=255)
                                 aTTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATT‑GTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATtgtg                                                                                                               >  1:136488/1‑150 (MQ=255)
                                    tGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGt                                                                                                             <  1:713433/150‑1 (MQ=255)
                                    tGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGt                                                                                                             >  1:699575/1‑150 (MQ=255)
                                      aaGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGttt                                                                                                           <  2:634755/150‑1 (MQ=255)
                                       aGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGtttt                                                                                                          >  2:928872/1‑150 (MQ=255)
                                        gCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTc                                                                                                         >  1:31839/1‑150 (MQ=255)
                                         cAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCa                                                                                                        <  1:292546/150‑1 (MQ=255)
                                         cAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCa                                                                                                        >  2:1509850/1‑150 (MQ=255)
                                          aaaaGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCaa                                                                                                       >  1:671603/1‑150 (MQ=255)
                                          aaaaGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCaa                                                                                                       >  1:603990/1‑150 (MQ=255)
                                          aaaaGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCaa                                                                                                       <  1:773200/150‑1 (MQ=255)
                                               gTTGGCAGACCTATGCCGTCGGTCTGGTGACGGGGGTGATTAGGTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGt                                                                                                  <  2:1194519/150‑1 (MQ=255)
                                                  ggCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTAt                                                                                               >  1:218252/1‑150 (MQ=255)
                                                  ggCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTAt                                                                                               >  2:143209/1‑150 (MQ=255)
                                                   ccAGACCTATGCAGTCGGTATGGTGACGAGGGTGATTAGTTTCTGGCTGTCGGGGTTGATTATTAGTCGTCGCGATGAAGAAAAAGAAAACGCACAATAAGAATATTATCGCGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATc                                                                                              >  2:1360158/2‑150 (MQ=255)
                                                    cAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATcc                                                                                             >  2:287380/1‑150 (MQ=255)
                                                          tATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCGATAATATTCTTATTGGGCGttttca                                                                                                                           >  2:845156/1‑110 (MQ=38)
                                                           aTGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGt                                                                                      >  2:158332/1‑150 (MQ=255)
                                                           aTGCCGTCGGTCTGGTGACGGGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGt                                                                                      <  2:163768/150‑1 (MQ=255)
                                                            tGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTg                                                                                     <  1:1195812/150‑1 (MQ=255)
                                                              ccGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGAc                                                                                   <  1:901408/150‑1 (MQ=255)
                                                                   ggTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGTGTTGATTATTGGTCGTAGCGATGAAGAAACTGAAAAGGCCCAATAAGAATATTATCGGGGGTTAAAATATTACATTGTTGTTTTCAATGCGTTATCCGCAGCGTGTCGCTcc                                                                              <  2:1009293/150‑1 (MQ=255)
                                                                   ggTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTcc                                                                              <  1:94749/150‑1 (MQ=255)
                                                                         gTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGcc                                                                        <  1:158332/150‑1 (MQ=255)
                                                                           gACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAg                                                                      >  2:2542/1‑150 (MQ=255)
                                                                              gTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtc                                                                   <  2:1563052/150‑1 (MQ=255)
                                                                              gTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtc                                                                   >  2:708318/1‑150 (MQ=255)
                                                                               tGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtca                                                                  >  2:181272/1‑150 (MQ=255)
                                                                                 ggTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtcaat                                                                >  2:1607587/1‑150 (MQ=255)
                                                                                    gATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtcaatcaa                                                             >  2:1031757/1‑150 (MQ=255)
                                                                                    gATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtcaatcaa                                                             >  2:759757/1‑150 (MQ=255)
                                                                                       tAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACg                                                          <  1:1509850/150‑1 (MQ=255)
                                                                                         gTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACgtg                                                        <  1:1492670/150‑1 (MQ=255)
                                                                                            tCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGa                                                     <  2:927263/150‑1 (MQ=255)
                                                                                             ctggctggCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGAt                                                    >  2:1407840/1‑150 (MQ=255)
                                                                                              tggctggcGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCG‑TATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCa                                                  <  1:546435/150‑1 (MQ=255)
                                                                                               ggctggcGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCa                                                  >  2:556099/1‑150 (MQ=255)
                                                                                               ggctggcGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCa                                                  <  1:287380/150‑1 (MQ=255)
                                                                                               ggctggcGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCa                                                  <  1:143209/150‑1 (MQ=255)
                                                                                               ggctggcGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCa                                                  >  1:573429/1‑150 (MQ=255)
                                                                                               ggctggcGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCa                                                  >  2:1333440/1‑150 (MQ=255)
                                                                                                     cGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCgg                                            >  2:633710/1‑150 (MQ=255)
                                                                                                     cGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCgg                                            >  1:1341509/1‑150 (MQ=255)
                                                                                                     cGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGAGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACTCTCCATCGCCAGTTCAATCAAACGTTTGATCAGATCgg                                            <  2:467369/150‑1 (MQ=255)
                                                                                                       gggTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGgtg                                          <  1:1031757/150‑1 (MQ=255)
                                                                                                       gggTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCAAATAAGAATATTATCGGGCGTTATAATATTAGATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGGGCCAGTTCAATCAAACGTGTGATCAGATCGgtg                                          <  1:1360158/150‑1 (MQ=255)
                                                                                                        ggTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGgtgt                                         <  2:44541/150‑1 (MQ=255)
                                                                                                        ggTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGgtgt                                         <  2:31839/150‑1 (MQ=255)
                                                                                                           tGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAAc                                      >  2:887896/1‑150 (MQ=255)
                                                                                                           tGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAAc                                      <  2:405091/150‑1 (MQ=255)
                                                                                                            gATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAAcc                                     >  2:276903/1‑150 (MQ=255)
                                                                                                             attattCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTTACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAAccc                                    >  2:695008/1‑150 (MQ=255)
                                                                                                               tattCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAg                                  <  1:740590/150‑1 (MQ=255)
                                                                                                                attCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGa                                 >  1:175285/1‑150 (MQ=255)
                                                                                                                      cgtcgCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTg                           <  1:556099/150‑1 (MQ=255)
                                                                                                                       gtcgCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTgg                          <  1:928872/150‑1 (MQ=255)
                                                                                                                         cgcgATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCt                        >  1:1223664/1‑150 (MQ=255)
                                                                                                                           cgATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGCCGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTg                      >  1:699778/1‑150 (MQ=255)
                                                                                                                           cgATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTg                      <  1:1086710/150‑1 (MQ=255)
                                                                                                                             aTGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGcc                    <  1:759757/150‑1 (MQ=255)
                                                                                                                             aTGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGcc                    <  1:744089/150‑1 (MQ=255)
                                                                                                                             aTGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGcc                    >  2:152736/1‑150 (MQ=255)
                                                                                                                              tGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCca                   <  1:1191919/150‑1 (MQ=255)
                                                                                                                                aagaaACTTAAAACTACAAATAAGAATATTATCTGGCGTTAAAATATTACATTGTGGTTTTCAATTATTTATCATCAGAGTTACGCTAAAGCGCCAGTTAAATCAAAATTTTGATCATATCGGTGTAACACAGAAAGCTGGCTTGCcaaa                 >  2:1589879/1‑148 (MQ=255)
                                                                                                                                aagaaACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCcaca                 >  1:889460/1‑150 (MQ=255)
                                                                                                                                 agaaACTGAACACCCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATGAAACGTGTGATCAGATCTGTGTAACCCAGACCGCTGGCTTGCCACAg                <  2:710574/150‑1 (MQ=255)
                                                                                                                                  gaaACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGc               >  1:308377/1‑150 (MQ=255)
                                                                                                                                       tGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCgg          <  1:2542/150‑1 (MQ=255)
                                                                                                                                       tGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCgg          <  1:1607587/150‑1 (MQ=255)
                                                                                                                                          aaaCGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGata       <  2:585372/150‑1 (MQ=255)
                                                                                                                                           aaCGCCCGATAATATTCTTATTGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGatac      >  2:1112447/1‑150 (MQ=255)
                                                                                                                                            aCGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGataca     <  2:603990/150‑1 (MQ=255)
                                                                                                                                               cccAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGatacatac  <  2:281647/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATAC  >  NC_000913/399655‑399947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑