Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,617,377 C→T 5.0% A121A (GCC→GCT) ‡ nikD → Ni(2(+)) ABC transporter ATP binding subunit NikD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,617,3770CT 5.0% 320.8 / 4.0 99A121A (GCC→GCT) ‡nikDNi(2(+)) ABC transporter ATP binding subunit NikD
Reads supporting (aligned to +/- strand):  ref base C (45/49);  new base T (2/3);  total (47/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01

Only 100 of 103 total aligned reads displayed.

TCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCT  >  NC_000913/3617228‑3617526
                                                                                                                                                     |                                                                                                                                                     
tCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGcc                                                                                                                                                       <  1:1048335/150‑1 (MQ=255)
tCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGcc                                                                                                                                                       <  2:1496778/150‑1 (MQ=255)
   ccTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATa                                                                                                                                                    <  2:1501234/150‑1 (MQ=255)
     ttGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGa                                                                                                                                                  >  2:1614578/1‑150 (MQ=255)
     ttGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGa                                                                                                                                                  <  2:75723/150‑1 (MQ=255)
     ttGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGa                                                                                                                                                  <  1:554119/150‑1 (MQ=255)
       gcgcCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACCCCCACGCGCGGGAACCCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACCCTTACCGCTGCCATAGCAg                                                                                                                                                >  1:1281750/1‑150 (MQ=255)
        cgcCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGc                                                                                                                                               >  2:882947/1‑150 (MQ=255)
         gcCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCg                                                                                                                                              >  1:787041/1‑150 (MQ=255)
            cTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTg                                                                                                                                           <  1:131804/150‑1 (MQ=255)
              gcgcGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTggg                                                                                                                                         <  1:1258236/150‑1 (MQ=255)
               cgcgGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTTCTATGGCAGCGGtaagc                                                                                                                                        <  1:752241/150‑5 (MQ=255)
               cgcgGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTTCTATGGCAGCGGtaagc                                                                                                                                        <  2:244577/150‑5 (MQ=255)
               cgcgGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTTCCATGGCAGCGGtaagc                                                                                                                                        <  1:673501/150‑5 (MQ=255)
                gcgGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTTCTATGGCAGCGGtaagcg                                                                                                                                       <  1:1594015/150‑6 (MQ=255)
                   gCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTgg                                                                                                                                    <  2:359180/150‑1 (MQ=255)
                    cATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGa                                                                                                                                   <  2:604127/150‑1 (MQ=255)
                       cAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGaaaa                                                                                                                                >  2:135165/1‑150 (MQ=255)
                       cAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGaaaa                                                                                                                                >  1:1113795/1‑150 (MQ=255)
                       cAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGaaaa                                                                                                                                >  1:20495/1‑150 (MQ=255)
                            ttGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGACGCGGTGGGGCTGGAAAAcgccg                                                                                                                           <  2:1589275/150‑1 (MQ=255)
                             tGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAAcgccgc                                                                                                                          <  1:831140/150‑1 (MQ=255)
                              gCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGGAGCGGTGGGGCTGGAAAACGCcgcg                                                                                                                         >  2:203244/1‑150 (MQ=255)
                              gCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCcgcg                                                                                                                         <  1:135165/150‑1 (MQ=255)
                              gCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCcgcg                                                                                                                         <  1:1609651/150‑1 (MQ=255)
                                 accaTCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCcgcgcgc                                                                                                                      >  2:270245/1‑150 (MQ=255)
                                   catcatGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGt                                                                                                                    >  2:1497693/1‑150 (MQ=255)
                                      catGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCt                                                                                                                 <  2:1622873/150‑1 (MQ=255)
                                       atGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTg                                                                                                                <  1:1554466/150‑1 (MQ=255)
                                        tGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGa                                                                                                               <  1:802750/150‑1 (MQ=255)
                                          cAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCATGCCGTTACGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAg                                                                                                             >  2:316099/1‑150 (MQ=255)
                                           aGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGc                                                                                                            <  1:614009/150‑1 (MQ=255)
                                           aGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGc                                                                                                            <  1:1096561/150‑1 (MQ=255)
                                            gAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCt                                                                                                           <  1:20105/150‑1 (MQ=255)
                                               cccGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTa                                                                                                        <  2:700323/150‑1 (MQ=255)
                                                 cgcgCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTAcc                                                                                                      >  1:847244/1‑150 (MQ=255)
                                                        cgcCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCga                                                                                               <  1:1497693/150‑1 (MQ=255)
                                                         gcCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCgag                                                                                              >  1:1247623/1‑150 (MQ=255)
                                                           cTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGAt                                                                                            >  2:1589187/1‑150 (MQ=255)
                                                             ttAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGa                                                                                          >  1:965666/1‑150 (MQ=255)
                                                              tAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAg                                                                                         >  2:1341388/1‑150 (MQ=255)
                                                              tAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAg                                                                                         <  2:1071432/150‑1 (MQ=255)
                                                                    aCTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGcggcgg                                                                                   <  2:236611/150‑1 (MQ=255)
                                                                     cTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCggcggc                                                                                  <  1:270245/150‑1 (MQ=255)
                                                                       gCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCAt                                                                                >  1:301856/1‑150 (MQ=255)
                                                                       gCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCAt                                                                                >  2:316577/1‑150 (MQ=255)
                                                                       gCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCAt                                                                                >  2:728903/1‑150 (MQ=255)
                                                                          cacCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGtt                                                                             >  2:757885/1‑150 (MQ=255)
                                                                            ccATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGc                                                                           <  2:847244/150‑1 (MQ=255)
                                                                                gCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAgcg                                                                       <  2:423345/150‑1 (MQ=255)
                                                                                gCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAgcg                                                                       <  2:1270557/150‑1 (MQ=255)
                                                                                 cacaCCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAgcgc                                                                      >  2:1611776/1‑150 (MQ=255)
                                                                                 cacaCCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAgcgc                                                                      >  1:1016410/1‑150 (MQ=255)
                                                                                   cacCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCat                                                                    <  1:287507/150‑1 (MQ=255)
                                                                                   cacCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGACATGTTGCAGCGCat                                                                    >  1:1181915/1‑150 (MQ=255)
                                                                                     cccACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCatga                                                                  >  1:880697/1‑150 (MQ=255)
                                                                                     cccACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCatga                                                                  >  2:1452637/1‑150 (MQ=255)
                                                                                     cccAAGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCatga                                                                  >  2:619328/1‑150 (MQ=255)
                                                                                       cACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCatgatg                                                                >  1:963012/1‑150 (MQ=255)
                                                                                         cgcgcgGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATgatgat                                                              >  1:1332443/1‑150 (MQ=255)
                                                                                           cgcgGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTg                                                            <  1:1614578/150‑1 (MQ=255)
                                                                                                   aCCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCg                                                    >  1:1416363/1‑150 (MQ=255)
                                                                                                    cctgcctgGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCgg                                                   >  2:598772/1‑150 (MQ=255)
                                                                                                       gcctgGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGc                                                <  1:168613/150‑1 (MQ=255)
                                                                                                         ctgGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCtg                                              <  2:508619/150‑1 (MQ=255)
                                                                                                           ggCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTGCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCtgtg                                            <  2:783510/150‑1 (MQ=255)
                                                                                                             cGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATTATGATTGCGATGGCGGTGCtgtgtg                                          <  2:20495/150‑1 (MQ=255)
                                                                                                             cGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCtgtgtg                                          >  1:941291/1‑150 (MQ=255)
                                                                                                             cGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCtgtgtg                                          >  2:540678/1‑150 (MQ=255)
                                                                                                             cGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCtgtgtg                                          <  2:1113795/150‑1 (MQ=255)
                                                                                                             cGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCtgtgtg                                          >  2:234762/1‑150 (MQ=255)
                                                                                                              gTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGa                                         <  2:1332443/150‑1 (MQ=255)
                                                                                                              gTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGa                                         >  1:1536814/1‑150 (MQ=255)
                                                                                                                tAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAAt                                       <  2:1016410/150‑1 (MQ=255)
                                                                                                                tAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAAt                                       >  1:323801/1‑150 (MQ=255)
                                                                                                                   ggAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCAc                                    <  1:1589187/150‑1 (MQ=255)
                                                                                                                    gAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCAcc                                   <  1:619328/150‑1 (MQ=255)
                                                                                                                    gAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGAGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAACCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCAcc                                   >  1:1331135/1‑150 (MQ=255)
                                                                                                                      aaCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGt                                 >  2:1075346/1‑150 (MQ=255)
                                                                                                                           gccgATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTatc                            <  2:1247623/150‑1 (MQ=255)
                                                                                                                            ccgATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTatca                           <  1:1075346/150‑1 (MQ=255)
                                                                                                                            ccgATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTatca                           <  1:316577/150‑1 (MQ=255)
                                                                                                                            ccgATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGACATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTatca                           <  2:1181915/150‑1 (MQ=255)
                                                                                                                               aTGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCg                        <  2:1349790/150‑1 (MQ=255)
                                                                                                                               aTGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCg                        <  1:644672/150‑1 (MQ=255)
                                                                                                                                 tacgctacgctTACCGCTTCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCACGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGcc                      <  2:1246855/149‑1 (MQ=255)
                                                                                                                                  acgctacgctTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCg                     <  1:882947/150‑1 (MQ=255)
                                                                                                                                     ctacgctTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATg                  <  1:540678/150‑1 (MQ=255)
                                                                                                                                      cagccccaCCGCTTCTATGGCAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGa                 >  1:1425396/8‑150 (MQ=255)
                                                                                                                                         ccccaCCGCTTCTATGGCAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAacc              >  1:1203348/5‑150 (MQ=255)
                                                                                                                                          ctTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAaccg             >  1:65168/1‑150 (MQ=255)
                                                                                                                                            tACCGCTGCCATAGAAGCGGTGGGGCTGGAAACCGCCGCGCTCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATGGCCGATGAaccgac           <  2:1281750/150‑1 (MQ=255)
                                                                                                                                               cGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAaccgaccac        <  2:410265/150‑1 (MQ=255)
                                                                                                                                               cGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAaccgaccac        >  1:1590325/1‑150 (MQ=255)
                                                                                                                                               cGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAaccgaccac        >  2:1043451/1‑150 (MQ=255)
                                                                                                                                                gCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAaccgaccacc       >  2:268884/1‑150 (MQ=255)
                                                                                                                                                gCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTACGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAaccgaccacc       >  1:101058/1‑150 (MQ=255)
                                                                                                                                                  tGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAaccgaccaccga     <  2:787041/150‑1 (MQ=255)
                                                                                                                                                     cATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCt  >  1:738683/1‑150 (MQ=255)
                                                                                                                                                     cATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCt  >  1:1031319/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCT  >  NC_000913/3617228‑3617526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑