Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,654,555 C→A 8.5% intergenic (+28/‑128) slp → / → dctR starvation lipoprotein/putative DNA‑binding transcriptional regulator DctR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,654,5550CA 8.5% 308.2 / 11.0 93intergenic (+28/‑128)slp/dctRstarvation lipoprotein/putative DNA‑binding transcriptional regulator DctR
Reads supporting (aligned to +/- strand):  ref base C (44/41);  new base A (3/5);  total (47/46)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTAATACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAGGG  >  NC_000913/3654407‑3654704
                                                                                                                                                    |                                                                                                                                                     
ggTTAATACCCCCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAAAGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCa                                                                                                                                                      >  2:1467146/1‑150 (MQ=255)
ggTTAATACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCa                                                                                                                                                      >  2:1187490/1‑150 (MQ=255)
    aaTACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCAt                                                                                                                                                  >  2:383756/1‑150 (MQ=255)
     aTACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATc                                                                                                                                                 >  2:1499697/1‑150 (MQ=255)
       accaccTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTg                                                                                                                                               <  1:1457972/150‑1 (MQ=255)
        ccaccTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGc                                                                                                                                              >  1:853457/1‑150 (MQ=255)
         caccTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGTGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTGAGAAAGCATCTGca                                                                                                                                             <  1:504703/150‑1 (MQ=255)
         caccTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGca                                                                                                                                             <  1:1261069/150‑1 (MQ=255)
         caccTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTGAGAAAGCATCTGca                                                                                                                                             <  1:1619712/150‑1 (MQ=255)
          accTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGcat                                                                                                                                            >  2:625435/1‑150 (MQ=255)
           ccTATAAGCTGTGGGATTAGGGCTATGGTGGATTGTGGCCGGAACCGGGCTGGGGGTAGCCTTACTAGACGAATGCGGTGAGTCAGGTAACAGGTGAGCTTGTCAAATAACACCACGGAAAGATGCAGATGGTGAGAAAGCATCTGcatc                                                                                                                                           <  2:312597/150‑1 (MQ=255)
           ccTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGcatc                                                                                                                                           <  2:865324/150‑1 (MQ=255)
           ccTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTGAGAAAGCATCTGcatc                                                                                                                                           <  1:1449483/150‑1 (MQ=255)
             tataACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGcatcat                                                                                                                                         >  2:1019347/1‑150 (MQ=255)
                aaCCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCa                                                                                                                                      >  1:791817/1‑150 (MQ=255)
                  ccTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCAtt                                                                                                                                    >  2:1124696/1‑150 (MQ=255)
                     gtgGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACa                                                                                                                                 >  1:149677/1‑150 (MQ=255)
                        ggATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCa                                                                                                                              <  2:1265899/150‑1 (MQ=255)
                         gATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCaa                                                                                                                             <  2:1127649/150‑1 (MQ=255)
                          aTTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCaaa                                                                                                                            <  1:1531211/150‑1 (MQ=255)
                            tACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGTGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAAtt                                                                                                                          >  2:811454/1‑150 (MQ=255)
                              cGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTaa                                                                                                                        >  2:1614218/1‑150 (MQ=255)
                                     ggTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTTAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAg                                                                                                                 <  2:336212/150‑1 (MQ=255)
                                     ggTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAg                                                                                                                 >  2:261604/1‑150 (MQ=255)
                                             cTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTaa                                                                                                         >  1:1106975/1‑150 (MQ=255)
                                                     aaCCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTATCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattatt                                                                                                 >  2:812763/1‑150 (MQ=255)
                                                      aCCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattatta                                                                                                <  2:856873/150‑1 (MQ=255)
                                                      aCCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattatta                                                                                                <  2:853457/150‑1 (MQ=255)
                                                       ccGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattattat                                                                                               <  1:1318281/150‑1 (MQ=255)
                                                       ccGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattattat                                                                                               >  1:180169/1‑150 (MQ=255)
                                                         gggCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattattatta                                                                                             <  1:353281/150‑1 (MQ=255)
                                                         gggCTGGGGTGCGCCTTACTACACCAATGCGGGGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattattatta                                                                                             <  2:970628/150‑1 (MQ=255)
                                                          ggCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattattattat                                                                                            >  1:1331678/1‑150 (MQ=255)
                                                            cTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAGTAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATaa                                                                                          >  2:1295513/1‑150 (MQ=255)
                                                              ggggTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGc                                                                                        <  2:1477143/150‑1 (MQ=255)
                                                                ggTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGccc                                                                                      >  1:891896/1‑150 (MQ=255)
                                                                 gTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCcct                                                                                     >  1:886686/1‑150 (MQ=255)
                                                                     gcCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCcctgtcc                                                                                 >  1:1420259/1‑150 (MQ=255)
                                                                     gcCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCcctgtcc                                                                                 <  2:1366199/150‑1 (MQ=255)
                                                                      ccTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCcctgtcct                                                                                <  2:180169/150‑1 (MQ=255)
                                                                       cTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAATTAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCcctgtcctg                                                                               <  2:431121/150‑1 (MQ=255)
                                                                         tactacACCAATGTGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGtt                                                                             <  1:811454/150‑1 (MQ=255)
                                                                         tactacACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAGTAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGtt                                                                             <  1:1295513/150‑1 (MQ=255)
                                                                          actacACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTa                                                                            <  2:835431/150‑1 (MQ=255)
                                                                          actacACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTa                                                                            >  1:20496/1‑150 (MQ=255)
                                                                             acacCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAAtt                                                                         <  2:345676/150‑1 (MQ=255)
                                                                             acacCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCACTGTACTGTTAAtt                                                                         >  2:1559247/1‑150 (MQ=255)
                                                                             acacCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTAAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATAAAATTAATACAAAGTAAGCTAACTATTATTATTATAAGCCATGTCATGTTAAtt                                                                         >  2:713907/1‑150 (MQ=255)
                                                                              cacCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTa                                                                        >  1:1133824/1‑150 (MQ=255)
                                                                              aacCAATGCGGTGAGTCAGGTAACACCTTAGCTGTTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTa                                                                        <  1:1596391/149‑1 (MQ=255)
                                                                                    tGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTg                                                                  <  2:149677/150‑1 (MQ=255)
                                                                                     gCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTgg                                                                 <  2:1514170/150‑1 (MQ=255)
                                                                                        gTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCaa                                                              >  2:198686/1‑150 (MQ=255)
                                                                                          gAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAAc                                                            >  1:1149332/1‑150 (MQ=255)
                                                                                          gAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAAc                                                            <  1:1499697/150‑1 (MQ=255)
                                                                                            gTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTg                                                          >  1:230414/1‑150 (MQ=255)
                                                                                            gTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTAAATTACCTTTGGCAAACTg                                                          >  2:272207/1‑150 (MQ=255)
                                                                                              cAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGAt                                                        >  1:727050/1‑150 (MQ=255)
                                                                                              cAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGAt                                                        <  2:791817/150‑1 (MQ=255)
                                                                                                     cacCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAAt                                                                          >  2:46142/1‑125 (MQ=255)
                                                                                                     cacCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAAt                                                                          <  1:46142/125‑1 (MQ=255)
                                                                                                       ccTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGtt                                               >  1:290345/1‑150 (MQ=255)
                                                                                                        cTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTa                                              >  1:1433907/1‑150 (MQ=255)
                                                                                                              tGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTAAGCATATGCATCATGCATTACATCAAATTAATACACATTAATCTAACTATTATTATTATAAGACCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGtta                                        <  1:1559247/150‑3 (MQ=255)
                                                                                                               ggTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCg                                       >  1:1444873/1‑150 (MQ=255)
                                                                                                               ggTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCg                                       <  2:842650/150‑1 (MQ=255)
                                                                                                                 tCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCa                                     >  1:924644/1‑150 (MQ=255)
                                                                                                                   aaaTAACACCACCTAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCAcc                                   <  1:625435/150‑1 (MQ=255)
                                                                                                                   aaaTAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCGTTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTAT‑AAGTTAATGTACccacca                                  >  1:219932/1‑150 (MQ=255)
                                                                                                                    aaTAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCa                                  <  1:1321062/150‑1 (MQ=255)
                                                                                                                     aTAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAg                                 <  1:1124696/150‑1 (MQ=255)
                                                                                                                        acacCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAg                              <  1:55461/150‑1 (MQ=255)
                                                                                                                         caccacCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGt                             >  2:1361152/1‑150 (MQ=255)
                                                                                                                         caccacCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGt                             >  2:1311637/1‑150 (MQ=255)
                                                                                                                             accGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTTATTATAAAGTTAATGTCCGCACCAGGAGTCGGt                         <  1:639951/150‑1 (MQ=255)
                                                                                                                             accGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGt                         <  1:1019347/150‑1 (MQ=255)
                                                                                                                             accGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGt                         <  1:294028/150‑1 (MQ=255)
                                                                                                                             accGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGt                         <  1:695022/150‑1 (MQ=255)
                                                                                                                              ccGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGtt                        >  2:894571/1‑150 (MQ=255)
                                                                                                                                gcatgatgcagatgcTGAGAAAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTAt                      >  2:1335409/5‑150 (MQ=255)
                                                                                                                                gAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTAt                      >  2:1391117/1‑150 (MQ=255)
                                                                                                                                gAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTAt                      >  1:848025/1‑150 (MQ=255)
                                                                                                                                     atgcagatgcTGAGAAAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGttta                 >  2:189837/1‑149 (MQ=255)
                                                                                                                                     atgcagatgcTGAGAAAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGttta                 >  1:1433428/1‑149 (MQ=255)
                                                                                                                                      tgcagatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCt                >  1:955709/1‑150 (MQ=255)
                                                                                                                                       gcagatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCtt               <  2:963027/150‑1 (MQ=255)
                                                                                                                                        cagatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAAt                                            >  1:852040/1‑120 (MQ=255)
                                                                                                                                        cagatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAAt                                            <  2:852040/120‑1 (MQ=255)
                                                                                                                                         agatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTtat             <  2:955709/150‑1 (MQ=255)
                                                                                                                                          gatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTtata            <  2:568997/150‑1 (MQ=255)
                                                                                                                                          gatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTtata            >  1:1169559/1‑150 (MQ=255)
                                                                                                                                           atgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATaa           <  2:1433907/150‑1 (MQ=255)
                                                                                                                                                ttCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTAcc      <  1:261604/150‑1 (MQ=255)
                                                                                                                                                  ctcAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTAATATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGAGTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAg    <  2:1438353/150‑1 (MQ=255)
                                                                                                                                                    cAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAggg  <  2:290345/150‑1 (MQ=255)
                                                                                                                                                    cAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGGCCTGTTAATTACCTTTGGCAAGCTGAGTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACAAggg  >  2:649903/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGTTAATACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAGGG  >  NC_000913/3654407‑3654704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑