Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,672,986 T→G 5.5% R191R (CGT→CGG)  yhjC → putative DNA‑binding transcriptional regulator YhjC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,672,9860TG 5.5% 287.8 / 6.9 91R191R (CGT→CGG) yhjCputative DNA‑binding transcriptional regulator YhjC
Reads supporting (aligned to +/- strand):  ref base T (41/45);  new base G (3/2);  total (44/47)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

GATACGCACTGGCGCGTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGAT  >  NC_000913/3672839‑3673135
                                                                                                                                                   |                                                                                                                                                     
gATACGCACTGGCGCGTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTta                                                                                                                                                     <  1:812635/150‑1 (MQ=255)
  tACGCACTGGCGCGTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTtata                                                                                                                                                   >  1:735283/1‑150 (MQ=255)
   aCGCACTGGCGCGTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATac                                                                                                                                                  <  2:983372/150‑1 (MQ=255)
        caggCGCGTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGc                                                                                                                                             >  1:1203636/3‑150 (MQ=255)
           gcgcGTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCAcc                                                                                                                                          <  1:333734/150‑1 (MQ=255)
            cgcgTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCt                                                                                                                                         >  1:389070/1‑150 (MQ=255)
               gTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCACACCTggg                                                                                                                                      <  1:963852/150‑1 (MQ=255)
               gTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGGTGTATAACGCActattg                                                                                                                                      <  2:362684/150‑6 (MQ=255)
                 tACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGgtg                                                                                                                                    <  1:1609749/150‑1 (MQ=255)
                 tACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGGTGTATAACGCActattgcg                                                                                                                                    <  1:1169794/150‑8 (MQ=255)
                  aCCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGgtgt                                                                                                                                   >  1:1400020/1‑150 (MQ=255)
                   ccGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTa                                                                                                                                  <  1:1578132/150‑1 (MQ=255)
                   ccGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTa                                                                                                                                  >  1:571056/1‑150 (MQ=255)
                           tGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGtt                                                                                                                          <  1:1173788/150‑1 (MQ=255)
                           tGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGtt                                                                                                                          >  2:284085/1‑150 (MQ=255)
                              cGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAgg                                                                                                                       >  2:250785/1‑150 (MQ=255)
                               gTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGt                                                                                                                      <  2:853274/150‑1 (MQ=255)
                                ttATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGtt                                                                                                                     >  1:512875/1‑150 (MQ=255)
                                  aTCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGtttt                                                                                                                   >  2:298966/1‑150 (MQ=255)
                                    cGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGa                                                                                                                 >  2:437044/1‑150 (MQ=255)
                                    cGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGa                                                                                                                 >  1:1108831/1‑150 (MQ=255)
                                     gCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAg                                                                                                                >  2:1254076/1‑150 (MQ=255)
                                     gCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAg                                                                                                                <  1:486225/150‑1 (MQ=255)
                                           ccccTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGcc                                                                                                          <  2:1554634/150‑1 (MQ=255)
                                           ccccTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGcc                                                                                                          <  2:1203636/150‑1 (MQ=255)
                                            cccTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCa                                                                                                         >  1:27421/1‑150 (MQ=255)
                                             ccTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAg                                                                                                        <  1:800024/150‑1 (MQ=255)
                                              cTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGc                                                                                                       <  2:763563/150‑1 (MQ=255)
                                              cTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGc                                                                                                       >  2:1368679/1‑150 (MQ=255)
                                                cGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGt                                                                                                     <  2:234704/150‑1 (MQ=255)
                                                 ggCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCGCACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGtt                                                                                                    >  2:201384/1‑150 (MQ=255)
                                                 gcacagttGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGtt                                                                                                    >  1:896277/8‑150 (MQ=255)
                                                  gCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTa                                                                                                   <  1:1252862/150‑1 (MQ=255)
                                                   cAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTaa                                                                                                  >  2:1378480/1‑150 (MQ=255)
                                                     aaCTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATg                                                                                                >  1:295898/1‑150 (MQ=255)
                                                     aaCTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATg                                                                                                <  1:770608/150‑1 (MQ=255)
                                                             aTGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAg                                                                                        <  1:288375/150‑1 (MQ=255)
                                                              tGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGt                                                                                       <  2:839398/150‑1 (MQ=255)
                                                               ggTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTg                                                                                      <  1:284085/150‑1 (MQ=255)
                                                                 gcAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTGATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGGTAATGGCGTCCAGTGGt                                                                                    <  2:1590854/149‑1 (MQ=255)
                                                                     cTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACCCGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTaa                                                                                >  2:1378869/1‑150 (MQ=255)
                                                                          ccAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGACGTTGCCAGCCTTAATCGCGTCCAGTGGTTTAAGTCTg                                                                           >  1:500567/1‑150 (MQ=255)
                                                                             gTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTggcg                                                                        <  2:1340733/150‑1 (MQ=255)
                                                                              tCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTggcgg                                                                       >  2:315892/1‑150 (MQ=255)
                                                                               ccGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTggcggc                                                                      >  2:580006/1‑150 (MQ=255)
                                                                                      aTCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGa                                                               >  2:46332/1‑150 (MQ=255)
                                                                                        cTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACg                                                             >  2:265913/1‑150 (MQ=255)
                                                                                         tGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACgg                                                            <  1:1404644/150‑1 (MQ=255)
                                                                                         tGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACgg                                                            >  2:16047/1‑150 (MQ=255)
                                                                                         tGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACgg                                                            <  1:437044/150‑1 (MQ=255)
                                                                                         tGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACgg                                                            >  1:288934/1‑150 (MQ=255)
                                                                                           aCGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAAt                                                                                                 <  2:1115338/111‑1 (MQ=255)
                                                                                           aCGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAAt                                                                                                 >  1:1115338/1‑111 (MQ=255)
                                                                                              cgcTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAAc                                                       <  1:265913/150‑1 (MQ=255)
                                                                                                 tttGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGt                                                    >  1:1464594/1‑150 (MQ=255)
                                                                                                    ggTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGc                                                 <  2:561108/150‑1 (MQ=255)
                                                                                                     gTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCg                                                <  1:410778/150‑1 (MQ=255)
                                                                                                     gTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCg                                                <  1:410775/150‑1 (MQ=255)
                                                                                                       tATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGaa                                              <  1:1368679/150‑1 (MQ=255)
                                                                                                          ccGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAAc                                           >  2:420028/1‑150 (MQ=255)
                                                                                                           cGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACt                                          >  1:887794/1‑150 (MQ=255)
                                                                                                           cGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACt                                          >  2:162970/1‑150 (MQ=255)
                                                                                                             cAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTAt                                        >  2:565246/1‑150 (MQ=255)
                                                                                                             cAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTAt                                        >  2:879206/1‑150 (MQ=255)
                                                                                                                  ccccGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCAc                                   <  2:896277/150‑1 (MQ=255)
                                                                                                                    ccGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCAccg                                 >  1:1321183/1‑150 (MQ=255)
                                                                                                                        tgatCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGTCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCgg                             <  1:1056342/150‑1 (MQ=255)
                                                                                                                        tgatCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCgg                             <  2:27421/150‑1 (MQ=255)
                                                                                                                          atCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTc                           <  2:1344090/150‑1 (MQ=255)
                                                                                                                          atCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTc                           >  1:842142/1‑150 (MQ=255)
                                                                                                                            cTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCtt                         >  1:711438/1‑150 (MQ=255)
                                                                                                                               aCTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGcc                      <  1:1378480/150‑1 (MQ=255)
                                                                                                                                 ttCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCgg                    <  2:735283/150‑1 (MQ=255)
                                                                                                                                   cacaCGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTc                  <  2:295898/150‑1 (MQ=255)
                                                                                                                                     cacGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCGAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTg                >  1:60822/1‑150 (MQ=255)
                                                                                                                                     cacGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTg                <  1:734785/150‑1 (MQ=255)
                                                                                                                                       cGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTggg              <  1:315892/150‑1 (MQ=255)
                                                                                                                                        gCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTgggg             <  1:231522/150‑1 (MQ=255)
                                                                                                                                         cccaggTGCGGTGTATAACGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGa            >  2:851352/6‑150 (MQ=255)
                                                                                                                                         cAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGa            >  2:154999/1‑150 (MQ=255)
                                                                                                                                         cAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGa            <  1:465530/150‑1 (MQ=255)
                                                                                                                                           caggTGCGGTGTATAACGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGatt          >  1:392691/4‑150 (MQ=255)
                                                                                                                                           caggTGCGGTGTATAACGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGatt          >  2:769428/4‑150 (MQ=255)
                                                                                                                                            tAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGatta         >  1:1261138/1‑150 (MQ=255)
                                                                                                                                               tGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTc      <  2:887794/150‑1 (MQ=255)
                                                                                                                                               tGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTc      <  2:1400020/150‑1 (MQ=255)
                                                                                                                                                 cGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAg    <  2:571056/150‑1 (MQ=255)
                                                                                                                                                 cGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAg    >  2:112367/1‑150 (MQ=255)
                                                                                                                                                 cGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAg    <  1:46332/150‑1 (MQ=255)
                                                                                                                                                  gTTTTACACCGCACCTTGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAATACTATCTCACCGCCTGTCTTGCCGGTCTTTGGATTATTgaga   <  2:1245629/150‑1 (MQ=255)
                                                                                                                                                  gTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGa   >  1:788339/1‑150 (MQ=255)
                                                                                                                                                   ttATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGAt  >  2:1185395/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GATACGCACTGGCGCGTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGAT  >  NC_000913/3672839‑3673135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑