Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,672,991 C→T 5.5% T193I (ACA→ATA)  yhjC → putative DNA‑binding transcriptional regulator YhjC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,672,9910CT 5.5% 286.1 / 4.0 90T193I (ACA→ATA) yhjCputative DNA‑binding transcriptional regulator YhjC
Reads supporting (aligned to +/- strand):  ref base C (42/43);  new base T (3/2);  total (45/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

ACGCACTGGCGCGTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGC  >  NC_000913/3672842‑3673140
                                                                                                                                                     |                                                                                                                                                     
aCGCACTGGCGCGTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATac                                                                                                                                                       <  2:983372/150‑1 (MQ=255)
     caggCGCGTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGc                                                                                                                                                  >  1:1203636/3‑150 (MQ=255)
        gcgcGTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCAcc                                                                                                                                               <  1:333734/150‑1 (MQ=255)
         cgcgTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCt                                                                                                                                              >  1:389070/1‑150 (MQ=255)
            gTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCACACCTggg                                                                                                                                           <  1:963852/150‑1 (MQ=255)
            gTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGGTGTATAACGCActattg                                                                                                                                           <  2:362684/150‑6 (MQ=255)
              tACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGgtg                                                                                                                                         <  1:1609749/150‑1 (MQ=255)
              tACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGGTGTATAACGCActattgcg                                                                                                                                         <  1:1169794/150‑8 (MQ=255)
               aCCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGgtgt                                                                                                                                        >  1:1400020/1‑150 (MQ=255)
                ccGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTa                                                                                                                                       <  1:1578132/150‑1 (MQ=255)
                ccGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTa                                                                                                                                       >  1:571056/1‑150 (MQ=255)
                        tGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGtt                                                                                                                               <  1:1173788/150‑1 (MQ=255)
                        tGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGtt                                                                                                                               >  2:284085/1‑150 (MQ=255)
                           cGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAgg                                                                                                                            >  2:250785/1‑150 (MQ=255)
                            gTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGt                                                                                                                           <  2:853274/150‑1 (MQ=255)
                             ttATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGtt                                                                                                                          >  1:512875/1‑150 (MQ=255)
                               aTCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGtttt                                                                                                                        >  2:298966/1‑150 (MQ=255)
                                 cGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGa                                                                                                                      >  1:1108831/1‑150 (MQ=255)
                                 cGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGa                                                                                                                      >  2:437044/1‑150 (MQ=255)
                                  gCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAg                                                                                                                     <  1:486225/150‑1 (MQ=255)
                                  gCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAg                                                                                                                     >  2:1254076/1‑150 (MQ=255)
                                        ccccTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGcc                                                                                                               <  2:1554634/150‑1 (MQ=255)
                                        ccccTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGcc                                                                                                               <  2:1203636/150‑1 (MQ=255)
                                         cccTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCa                                                                                                              >  1:27421/1‑150 (MQ=255)
                                          ccTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAg                                                                                                             <  1:800024/150‑1 (MQ=255)
                                           cTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGc                                                                                                            >  2:1368679/1‑150 (MQ=255)
                                           cTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGc                                                                                                            <  2:763563/150‑1 (MQ=255)
                                             cGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGt                                                                                                          <  2:234704/150‑1 (MQ=255)
                                              ggCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCGCACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGtt                                                                                                         >  2:201384/1‑150 (MQ=255)
                                              gcacagttGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGtt                                                                                                         >  1:896277/8‑150 (MQ=255)
                                               gCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTa                                                                                                        <  1:1252862/150‑1 (MQ=255)
                                                cAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTaa                                                                                                       >  2:1378480/1‑150 (MQ=255)
                                                  aaCTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATg                                                                                                     <  1:770608/150‑1 (MQ=255)
                                                  aaCTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATg                                                                                                     >  1:295898/1‑150 (MQ=255)
                                                          aTGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAg                                                                                             <  1:288375/150‑1 (MQ=255)
                                                           tGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGt                                                                                            <  2:839398/150‑1 (MQ=255)
                                                            ggTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTg                                                                                           <  1:284085/150‑1 (MQ=255)
                                                              gcAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTGATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGGTAATGGCGTCCAGTGGt                                                                                         <  2:1590854/149‑1 (MQ=255)
                                                                  cTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACCCGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTaa                                                                                     >  2:1378869/1‑150 (MQ=255)
                                                                       ccAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGACGTTGCCAGCCTTAATCGCGTCCAGTGGTTTAAGTCTg                                                                                >  1:500567/1‑150 (MQ=255)
                                                                          gTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTggcg                                                                             <  2:1340733/150‑1 (MQ=255)
                                                                           tCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTggcgg                                                                            >  2:315892/1‑150 (MQ=255)
                                                                            ccGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTggcggc                                                                           >  2:580006/1‑150 (MQ=255)
                                                                                   aTCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGa                                                                    >  2:46332/1‑150 (MQ=255)
                                                                                     cTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACg                                                                  >  2:265913/1‑150 (MQ=255)
                                                                                      tGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACgg                                                                 <  1:437044/150‑1 (MQ=255)
                                                                                      tGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACgg                                                                 >  2:16047/1‑150 (MQ=255)
                                                                                      tGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACgg                                                                 >  1:288934/1‑150 (MQ=255)
                                                                                      tGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACgg                                                                 <  1:1404644/150‑1 (MQ=255)
                                                                                        aCGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAAt                                                                                                      >  1:1115338/1‑111 (MQ=255)
                                                                                        aCGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAAt                                                                                                      <  2:1115338/111‑1 (MQ=255)
                                                                                           cgcTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAAc                                                            <  1:265913/150‑1 (MQ=255)
                                                                                              tttGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGt                                                         >  1:1464594/1‑150 (MQ=255)
                                                                                                 ggTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGc                                                      <  2:561108/150‑1 (MQ=255)
                                                                                                  gTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCg                                                     <  1:410775/150‑1 (MQ=255)
                                                                                                  gTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCg                                                     <  1:410778/150‑1 (MQ=255)
                                                                                                    tATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGaa                                                   <  1:1368679/150‑1 (MQ=255)
                                                                                                       ccGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAAc                                                >  2:420028/1‑150 (MQ=255)
                                                                                                        cGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACt                                               >  2:162970/1‑150 (MQ=255)
                                                                                                        cGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACt                                               >  1:887794/1‑150 (MQ=255)
                                                                                                          cAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTAt                                             >  2:879206/1‑150 (MQ=255)
                                                                                                          cAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTAt                                             >  2:565246/1‑150 (MQ=255)
                                                                                                               ccccGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCAc                                        <  2:896277/150‑1 (MQ=255)
                                                                                                                 ccGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCAccg                                      >  1:1321183/1‑150 (MQ=255)
                                                                                                                     tgatCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGTCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCgg                                  <  1:1056342/150‑1 (MQ=255)
                                                                                                                     tgatCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCgg                                  <  2:27421/150‑1 (MQ=255)
                                                                                                                       atCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTc                                <  2:1344090/150‑1 (MQ=255)
                                                                                                                       atCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTc                                >  1:842142/1‑150 (MQ=255)
                                                                                                                         cTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCtt                              >  1:711438/1‑150 (MQ=255)
                                                                                                                            aCTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGcc                           <  1:1378480/150‑1 (MQ=255)
                                                                                                                              ttCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCgg                         <  2:735283/150‑1 (MQ=255)
                                                                                                                                cacaCGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTc                       <  2:295898/150‑1 (MQ=255)
                                                                                                                                  cacGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCGAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTg                     >  1:60822/1‑150 (MQ=255)
                                                                                                                                  cacGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTg                     <  1:734785/150‑1 (MQ=255)
                                                                                                                                    cGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTggg                   <  1:315892/150‑1 (MQ=255)
                                                                                                                                     gCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTgggg                  <  1:231522/150‑1 (MQ=255)
                                                                                                                                      cccaggTGCGGTGTATAACGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGa                 >  2:851352/6‑150 (MQ=255)
                                                                                                                                      cAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGa                 <  1:465530/150‑1 (MQ=255)
                                                                                                                                      cAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGa                 >  2:154999/1‑150 (MQ=255)
                                                                                                                                        caggTGCGGTGTATAACGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGatt               >  2:769428/4‑150 (MQ=255)
                                                                                                                                        caggTGCGGTGTATAACGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGatt               >  1:392691/4‑150 (MQ=255)
                                                                                                                                         tAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGatta              >  1:1261138/1‑150 (MQ=255)
                                                                                                                                            tGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTc           <  2:1400020/150‑1 (MQ=255)
                                                                                                                                            tGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTc           <  2:887794/150‑1 (MQ=255)
                                                                                                                                              cGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAg         <  2:571056/150‑1 (MQ=255)
                                                                                                                                              cGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAg         >  2:112367/1‑150 (MQ=255)
                                                                                                                                              cGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAg         <  1:46332/150‑1 (MQ=255)
                                                                                                                                               gTTTTACACCGCACCTTGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAATACTATCTCACCGCCTGTCTTGCCGGTCTTTGGATTATTgaga        <  2:1245629/150‑1 (MQ=255)
                                                                                                                                               gTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGa        >  1:788339/1‑150 (MQ=255)
                                                                                                                                                ttATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGAt       >  2:1185395/1‑150 (MQ=255)
                                                                                                                                                   taCACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATccc    >  2:1526514/1‑150 (MQ=255)
                                                                                                                                                    acacCGCACCTGGGTGTACATCCCTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCAAGTGGATTAAGTCTGGCTGCATGTAGACGGTAAACAGTAGCGAAAAGTTTCTCAAAGCATGTCTAGCGGGTCTGGGGATTATTCAGATCCcg   >  2:878761/1‑150 (MQ=255)
                                                                                                                                                     cacCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCcgc  <  1:201384/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACGCACTGGCGCGTTACCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGC  >  NC_000913/3672842‑3673140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑