Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,689,724 G→C 5.5% P180A (CCC→GCC)  bcsZ ← endo‑1,4‑D‑glucanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,689,7240GC 5.5% 302.9 / 6.7 91P180A (CCC→GCC) bcsZendo‑1,4‑D‑glucanase
Reads supporting (aligned to +/- strand):  ref base G (38/47);  new base C (2/3);  total (40/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

GCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCTTTCCACA  >  NC_000913/3689575‑3689870
                                                                                                                                                     |                                                                                                                                                  
gCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTggg                                                                                                                                                    >  1:243730/1‑150 (MQ=255)
  cTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGt                                                                                                                                                  <  1:1418770/150‑1 (MQ=255)
   tttGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGtt                                                                                                                                                 <  1:1308367/150‑1 (MQ=255)
    ttGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTa                                                                                                                                                <  1:812380/150‑1 (MQ=255)
      gTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTaaa                                                                                                                                              <  1:985895/150‑1 (MQ=255)
      gTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGCGtttta                                                                                                                                              >  2:818556/1‑147 (MQ=255)
       tCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTaaaa                                                                                                                                             <  1:168215/150‑1 (MQ=255)
        cTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGTGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAAc                                                                                                                                            >  2:1594798/1‑150 (MQ=255)
           tctcATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGcc                                                                                                                                         <  2:49874/150‑1 (MQ=255)
              cATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGc                                                                                                                                      >  2:973134/1‑150 (MQ=255)
              cATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGCGTTTTAACCCCAGc                                                                                                                                      <  1:818556/150‑1 (MQ=255)
              cATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGCTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGc                                                                                                                                      >  2:399215/1‑150 (MQ=255)
                tAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGCGTTTTAACCCCAGCta                                                                                                                                    <  2:1292204/150‑2 (MQ=255)
                 aGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGt                                                                                                                                   <  1:570520/150‑1 (MQ=255)
                 aGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGCGTTTTAACCCCAGCtac                                                                                                                                   <  2:112294/150‑3 (MQ=255)
                   cgcACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTa                                                                                                                                 <  2:93429/150‑1 (MQ=255)
                   cgcACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTa                                                                                                                                 <  2:1624135/150‑1 (MQ=255)
                    gcACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTAt                                                                                                                                >  2:1464990/1‑150 (MQ=255)
                    gcACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGATCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTAt                                                                                                                                <  1:587200/150‑1 (MQ=255)
                      aCCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATcc                                                                                                                              <  1:598355/150‑1 (MQ=255)
                       cccAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCt                                                                                                                             >  2:614802/1‑150 (MQ=255)
                          aGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAg                                                                                                                          <  1:651539/150‑1 (MQ=255)
                          aGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAg                                                                                                                          <  2:930015/150‑1 (MQ=255)
                             cTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCaa                                                                                                                       >  2:582514/1‑150 (MQ=255)
                                gCGAAAAACCTTTCGGGGCGGTTTCCGGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAAc                                                                                                                    >  2:605052/1‑150 (MQ=255)
                                gCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAAc                                                                                                                    <  2:454927/150‑1 (MQ=255)
                                 cGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAAcc                                                                                                                   >  1:1134826/1‑150 (MQ=255)
                                 cGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAAcc                                                                                                                   <  1:1118972/150‑1 (MQ=255)
                                 cGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAAcc                                                                                                                   >  1:397349/1‑150 (MQ=255)
                                  gAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAAccc                                                                                                                  <  1:973134/150‑1 (MQ=255)
                                  gAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAAccc                                                                                                                  <  1:1017101/150‑1 (MQ=255)
                                  gAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAAccc                                                                                                                  <  1:1224453/150‑1 (MQ=255)
                                        ccTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTg                                                                                                            >  2:1292790/1‑150 (MQ=255)
                                        ccTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTg                                                                                                            <  2:54057/150‑1 (MQ=255)
                                          tttCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGcc                                                                                                          >  1:1033500/1‑150 (MQ=255)
                                           ttCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGCTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGccc                                                                                                         <  2:507434/150‑1 (MQ=255)
                                             cGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCgg                                                                                                       <  2:1145345/150‑1 (MQ=255)
                                              ggggCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCCCGGCGCGCCAAAGCGGGTGAAATCCTGCGCCAGCGTCGGCCGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCCCGTTGCCCGGt                                                                                                      >  2:1505451/1‑150 (MQ=255)
                                              ggggCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGt                                                                                                      <  1:881725/150‑1 (MQ=255)
                                               gggCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTa                                                                                                     <  1:1060714/150‑1 (MQ=255)
                                               gggCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTa                                                                                                     >  2:691178/1‑150 (MQ=255)
                                               gggCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAACAAAACCCACTTTGCCCGGTa                                                                                                     <  2:134236/150‑1 (MQ=255)
                                               gggCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTAGCCTCAGCAAAACCCACTTTGCCCGGTa                                                                                                     >  2:1025079/1‑150 (MQ=255)
                                               gggCGGGTTCCAGCAATAAAGGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCTCCAGCGTCTGCGGCAGGTAGCTAGTGTTAAAACGCTAGCTGTTATCCTCAGCAAAACCCACTTTGCGCTGTa                                                                                                     <  2:132591/150‑1 (MQ=255)
                                                 gCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTaac                                                                                                   <  1:81342/150‑1 (MQ=255)
                                                 gCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTaac                                                                                                   >  2:1289429/1‑150 (MQ=255)
                                                       tcgaGCAATCATCGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGGCAGCGTCGGCGGGATGTAGCTGGGGGTAAAACGCCAGCTGTTATCCTCAGCAAAAGCCACTTTGCCCGGTAACAACTTg                                                                                             <  2:1520264/147‑1 (MQ=255)
                                                         cAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGa                                                                                           >  1:1139516/1‑150 (MQ=255)
                                                                aaaCGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAAcccagc                                                                                    <  2:493/150‑1 (MQ=255)
                                                                aaaCGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAAcccagc                                                                                    >  2:870014/1‑150 (MQ=255)
                                                                      tGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGc                                                                              <  1:1043762/150‑1 (MQ=255)
                                                                       gATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCa                                                                             >  1:1597748/1‑150 (MQ=255)
                                                                         ttGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAATCCCTCTTTGCCCGGTAACAACTTGGTACCCAGACCAGGCAct                                                                           <  2:1478365/150‑2 (MQ=255)
                                                                             tttCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTca                                                                       <  2:243730/150‑1 (MQ=255)
                                                                              ttCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTcac                                                                      >  2:119928/1‑150 (MQ=255)
                                                                                cgcgCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTcacca                                                                    >  2:673694/1‑150 (MQ=255)
                                                                                cgcgCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCACCAAAACCCACTTTGCCCGGTAACAACATGGAACCCCGCCCAGGCACCGTcaccc                                                                    >  2:845599/1‑149 (MQ=255)
                                                                                   gcAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACtt                                                                 <  2:1262818/150‑1 (MQ=255)
                                                                                     aGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTtcc                                                               <  1:582514/150‑1 (MQ=255)
                                                                                      gCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTtcct                                                              >  2:207760/1‑150 (MQ=255)
                                                                                          ggTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCcg                                                          >  1:1368139/1‑150 (MQ=255)
                                                                                              cACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCcgcgcg                                                      <  2:1064674/150‑1 (MQ=255)
                                                                                                   cgcgcCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACg                                                 <  2:78944/150‑1 (MQ=255)
                                                                                                     cgcCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTcacc                                                                     >  2:1149752/1‑128 (MQ=255)
                                                                                                     cgcCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTcacc                                                                     <  1:1149752/128‑1 (MQ=255)
                                                                                                       ccAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGtttt                                             <  2:1033500/150‑1 (MQ=255)
                                                                                                        cAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCAACGTAACCACATCCTCCCGCGCGATACGTTTTa                                            >  2:711142/1‑150 (MQ=255)
                                                                                                         aaaGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAg                                           <  1:673694/150‑1 (MQ=255)
                                                                                                          aaGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGc                                          <  2:1134826/150‑1 (MQ=255)
                                                                                                             cGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAAc                                       >  2:1584253/1‑150 (MQ=255)
                                                                                                               ggTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAACCCCACTGTGCCCGGTAACCACATGGAACCCAGCCCAGGCCCCGTCCCCCCGTCCTCCCGCGCGATACGGTTTAGCCAcgc                                     >  2:771032/1‑150 (MQ=255)
                                                                                                                      tACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGccc                              >  2:200409/1‑149 (MQ=255)
                                                                                                                      tACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACATTTTAGCAACGCGCTGCCg                              <  1:1065810/150‑1 (MQ=255)
                                                                                                                       aCTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGa                             >  1:809687/1‑150 (MQ=255)
                                                                                                                          gcgcCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACCTGGAACCCAGCCCAGGCCCCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGt                          >  2:178755/1‑150 (MQ=255)
                                                                                                                           cgcCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTc                         >  1:17154/1‑150 (MQ=255)
                                                                                                                           cgcCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTc                         >  2:348130/1‑150 (MQ=255)
                                                                                                                             ccAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCgg                       <  1:207760/150‑1 (MQ=255)
                                                                                                                             ccAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCgg                       <  1:1292790/150‑1 (MQ=255)
                                                                                                                             ccAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCgg                       <  1:13447/150‑1 (MQ=255)
                                                                                                                              cAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGt                      <  1:473945/150‑1 (MQ=255)
                                                                                                                                  gTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCCGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATaa                  >  2:1610192/1‑150 (MQ=255)
                                                                                                                                  gTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATaa                  >  1:166326/1‑150 (MQ=255)
                                                                                                                                     ggcggcAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAACgc               >  2:1272956/1‑150 (MQ=255)
                                                                                                                                     ggcggcAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTCCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTACCTCCCGCGCGATACGTTTTAGCAACGCGCTCCCGATGTCGGTATAACgc               >  2:503926/1‑150 (MQ=255)
                                                                                                                                     ggcggcAGGTAGCTCGGGTTAAAACGCCAGCTGTAATCCTCAGCAAAACCCACTTTCCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTACCACCCGCGCGATACGTATTAGCAACGAGCTCCCCATGACGGAATAACgc               >  2:498402/1‑150 (MQ=255)
                                                                                                                                         gcAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCACGCACCGTCACCACTTCCTCCCGCGCGATACCTTTTAGCAACGCCCTGCCGATGTCGGTATAACgctcca           >  1:1547179/1‑147 (MQ=255)
                                                                                                                                             gTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCttt       <  2:1368139/150‑1 (MQ=255)
                                                                                                                                             acaGCTGGCGTTTTAACCCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGTCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCttt       >  1:1316996/3‑150 (MQ=255)
                                                                                                                                              tAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAACCCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCTTTc      <  1:399215/150‑1 (MQ=255)
                                                                                                                                               aGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCTTTcc     <  1:1555710/150‑1 (MQ=255)
                                                                                                                                                  tGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCTTTCcaca  <  2:397349/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAACGCTGCTCTTTCCACA  >  NC_000913/3689575‑3689870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑