Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,728,215 T→A 10.5% K386I (AAA→ATA)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,728,2150TA10.5% 159.6 / 9.2 57K386I (AAA→ATA) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base T (23/28);  new base A (4/2);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01

GCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACG  >  NC_000913/3728068‑3728363
                                                                                                                                                   |                                                                                                                                                    
gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTa                                                                                                                                                    <  1:1332469/150‑1 (MQ=255)
            aGCCTTGCTGCGCCCAGTGGTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTATTAAACCGCaaagt                                                                                                                                        <  2:1010449/150‑5 (MQ=255)
            aGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGTATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa                                                                                                                                        <  2:441056/150‑1 (MQ=255)
              ccTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg                                                                                                                                      <  1:1014181/150‑1 (MQ=255)
              ccTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTATTAAACCGCaaagtgt                                                                                                                                      <  1:379496/150‑7 (MQ=255)
                        cccAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc                                                                                                                            <  2:238066/150‑1 (MQ=255)
                           aGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATg                                                                                                                         <  2:834208/150‑1 (MQ=255)
                                  gCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGAACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCTGTTTAATACCGCAGGCATGGATGTCGTGCATGcctgcgg                                                                                                                  <  1:1485165/150‑6 (MQ=255)
                                           ccccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCAt                                                                                                         <  2:30808/150‑1 (MQ=255)
                                           ccccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCAt                                                                                                         <  2:1390945/150‑1 (MQ=255)
                                                     aCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGcc                                                                                               <  2:1551621/150‑1 (MQ=255)
                                                              taGCTGCTGTCCGCTGATATCCTCCAGCTTCTGACGCCAGTACTGACTAGGCGCCCCGCGCAGAATGAACGTAACACTTTGGTTTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCTCATAACCCACGCGTTCCAGCAc                                                                                      <  2:603193/149‑1 (MQ=255)
                                                              gAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCAc                                                                                      <  1:440186/150‑1 (MQ=255)
                                                              gAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCAc                                                                                      >  1:126218/1‑150 (MQ=255)
                                                               aGCTGCTGACCGCTGATATACGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCAca                                                                                     >  1:603193/1‑149 (MQ=255)
                                                                gctgctGACCGCTGATATCTGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGTCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGc                                                                                        <  2:1390711/146‑1 (MQ=255)
                                                                gctgctGACCGCTGATATCTGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGc                                                                                        >  1:1390711/1‑146 (MQ=255)
                                                                 ctgctgACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGc                                                                                   >  2:1068801/1‑150 (MQ=255)
                                                                   gctgACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTc                                                                                 <  1:785497/150‑1 (MQ=255)
                                                                   gctgACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTc                                                                                 >  2:1148811/1‑150 (MQ=255)
                                                                        ccGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGcc                                                                            <  1:1555697/150‑1 (MQ=255)
                                                                            tGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGtt                                                                        <  2:803906/150‑1 (MQ=255)
                                                                                 tCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTg                                                                   <  1:1068801/150‑1 (MQ=255)
                                                                                           tCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGtt                                                         >  2:1532379/1‑150 (MQ=255)
                                                                                           tCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGtt                                                         <  1:1366829/150‑1 (MQ=255)
                                                                                              gACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTtgat                                                      <  2:567982/150‑1 (MQ=255)
                                                                                                cGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTtgatga                                                    >  2:186533/1‑150 (MQ=255)
                                                                                                 gCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAg                                                   >  1:227486/1‑150 (MQ=255)
                                                                                                    aGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCa                                                >  2:458549/1‑150 (MQ=255)
                                                                                                       aCTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAAc                                             <  1:705356/150‑1 (MQ=255)
                                                                                                        cTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAAcc                                            <  2:546318/150‑1 (MQ=255)
                                                                                                        cTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAAcc                                            <  1:836456/150‑1 (MQ=255)
                                                                                                          cACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCCGGGCATACCCCCCGCGTACCAGCACTGCTCGGGCCGGTACATTGGGGCCATGTTGTGGAGGCAACCCaa                                          >  2:358431/1‑150 (MQ=255)
                                                                                                          cACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCaa                                          >  1:767798/1‑150 (MQ=255)
                                                                                                           aCTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCaaa                                         <  1:363235/150‑1 (MQ=255)
                                                                                                           aCTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCaaa                                         <  1:2728/150‑1 (MQ=255)
                                                                                                                 cgccccgcccCAAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAAc                                   <  1:1439706/150‑1 (MQ=255)
                                                                                                                    cccgccccCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAAcccc                                >  2:1502395/1‑150 (MQ=255)
                                                                                                                        cccccAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTc                            >  2:447160/1‑150 (MQ=255)
                                                                                                                           ccAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGcc                         <  2:714128/150‑1 (MQ=255)
                                                                                                                             aaTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTg                       <  1:1532379/150‑1 (MQ=255)
                                                                                                                               tCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCATTCGCCTggg                     <  1:1561936/150‑1 (MQ=255)
                                                                                                                                 aaCGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGa                   >  2:1454126/1‑150 (MQ=255)
                                                                                                                                     tAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGattat               >  1:1535943/1‑150 (MQ=255)
                                                                                                                                        cacTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgt            >  1:258569/1‑150 (MQ=255)
                                                                                                                                        cacTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTCCTCGCTCCAGTTCATTGGAGCCATGTTGATGAGTCAAACCAAATAAAACCCCCATCGCCTGCGGattattat            >  2:288313/1‑148 (MQ=255)
                                                                                                                                         tgcctGCGGTATTAAACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtg           >  1:667854/5‑150 (MQ=255)
                                                                                                                                         acTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtg           >  1:645897/1‑150 (MQ=255)
                                                                                                                                          cTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtgt          >  1:714588/1‑150 (MQ=255)
                                                                                                                                           cctGCGGTATTAAACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtgtg         >  1:1004103/3‑150 (MQ=255)
                                                                                                                                           cctGCGGTATTAAACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtgtg         >  1:917123/3‑150 (MQ=255)
                                                                                                                                           cctGCGGTATTAAACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtgtg         >  1:199405/3‑150 (MQ=255)
                                                                                                                                            ttGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTgg        <  1:186533/150‑1 (MQ=255)
                                                                                                                                             tGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGc       >  2:1309197/1‑150 (MQ=255)
                                                                                                                                              gCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCg      >  1:719250/1‑150 (MQ=255)
                                                                                                                                                ggTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTa    >  1:1227626/1‑150 (MQ=255)
                                                                                                                                                 gTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTAc   >  2:115247/1‑150 (MQ=255)
                                                                                                                                                  tttAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGTGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACg  >  2:1177606/1‑150 (MQ=255)
                                                                                                                                                  tttAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACg  <  1:1148811/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACG  >  NC_000913/3728068‑3728363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑