Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,748,111 T→G 5.9% L343R (CTG→CGG) ‡ lyxK → L‑xylulose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,748,1110TG 5.9% 269.0 / 7.1 84L343R (CTG→CGG) ‡lyxKL‑xylulose kinase
Reads supporting (aligned to +/- strand):  ref base T (37/42);  new base G (3/2);  total (40/44)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTT  >  NC_000913/3747963‑3748255
                                                                                                                                                    |                                                                                                                                                
gAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTg                                                                                                                                                 <  2:110159/150‑1 (MQ=255)
 aaTTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGt                                                                                                                                                >  2:1199845/1‑150 (MQ=255)
  aTTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTa                                                                                                                                               <  1:216136/150‑1 (MQ=255)
  aTTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTa                                                                                                                                               >  1:1591949/1‑150 (MQ=255)
  aTTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTa                                                                                                                                               <  2:1125140/150‑1 (MQ=255)
   tttATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTAc                                                                                                                                              >  2:313934/1‑150 (MQ=255)
   tttATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTAc                                                                                                                                              >  2:319793/1‑150 (MQ=255)
    ttATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAGTGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACg                                                                                                                                             <  1:371807/150‑1 (MQ=255)
      aTCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgc                                                                                                                                           >  2:625202/1‑150 (MQ=255)
      aTCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgc                                                                                                                                           >  1:1106284/1‑150 (MQ=255)
         gTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGATTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgcagc                                                                                                                                        >  2:834205/1‑150 (MQ=255)
          ttCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgcagca                                                                                                                                       <  1:1631898/150‑1 (MQ=255)
           tCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCaa                                                                                                                                      >  1:4387/1‑150 (MQ=255)
           tCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCaa                                                                                                                                      <  1:1306152/150‑1 (MQ=255)
            cACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGGCAGGaaa                                                                                                                                     <  2:1456388/150‑2 (MQ=255)
              cGAAGCCAGCCCTACCTCTTCCGGCAACCACGAATGGTTTACCGCACAGAGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCAGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGc                                                                                                                                   >  2:1144232/1‑150 (MQ=255)
               gAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGcc                                                                                                                                  <  1:117842/150‑1 (MQ=255)
               gAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGGCAGGaaaaag                                                                                                                                  <  1:1052253/150‑5 (MQ=255)
                       cccTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCga                                                                                                                          <  1:350001/150‑1 (MQ=255)
                        ccTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCgag                                                                                                                         >  2:345722/1‑150 (MQ=255)
                        ccTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCgag                                                                                                                         <  1:1168102/150‑1 (MQ=255)
                          tACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGAt                                                                                                                       <  1:20839/150‑1 (MQ=255)
                            ccTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGa                                                                                                                     >  2:511733/1‑150 (MQ=255)
                             ctctTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGAc                                                                                                                    >  2:857559/1‑150 (MQ=255)
                              tctTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGCCTCGAGATGAcc                                                                                                                   >  1:1354588/1‑150 (MQ=255)
                               ctTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCa                                                                                                                  >  2:1014037/1‑150 (MQ=255)
                               ctTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCa                                                                                                                  >  2:1306596/1‑150 (MQ=255)
                                          cTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTAc                                                                                                       <  1:1306596/150‑1 (MQ=255)
                                           tCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACg                                                                                                      <  2:769055/150‑1 (MQ=255)
                                                tGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATg                                                                                                 >  1:671417/1‑150 (MQ=255)
                                                  gTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCa                                                                                               >  1:995420/1‑150 (MQ=255)
                                                  gTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCa                                                                                               <  2:1630145/150‑1 (MQ=255)
                                                   tttACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGCGGTTTCTACGGGATGCAg                                                                                              >  1:1543469/1‑150 (MQ=255)
                                                    ttACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAgg                                                                                             <  1:319793/150‑1 (MQ=255)
                                                    ttACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAgg                                                                                             <  1:313934/150‑1 (MQ=255)
                                                     tACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGc                                                                                            <  1:91771/150‑1 (MQ=255)
                                                      aCCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGcc                                                                                           >  2:1159935/1‑150 (MQ=255)
                                                       ccGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCATGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCa                                                                                          >  2:1107947/1‑150 (MQ=255)
                                                        cGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCAt                                                                                         >  2:539175/1‑150 (MQ=255)
                                                         gCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCAtt                                                                                        >  2:293401/1‑150 (MQ=255)
                                                           acaGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTca                                                                                      <  1:625202/150‑1 (MQ=255)
                                                                ggggAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACAccc                                                                                 >  2:463145/1‑150 (MQ=255)
                                                                  ggAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCcgc                                                                               >  1:1514974/1‑150 (MQ=255)
                                                                  ggAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCcgc                                                                               <  1:1171597/150‑1 (MQ=255)
                                                                  ggAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAAGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCcgc                                                                               >  2:1017075/1‑150 (MQ=255)
                                                                     gaAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCcgcgcg                                                                            >  1:296379/1‑150 (MQ=255)
                                                                         tctcGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCAcc                                                                        <  1:1159935/150‑1 (MQ=255)
                                                                          ctcGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCt                                                                       <  2:4387/150‑1 (MQ=255)
                                                                               ttGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGc                                                                  <  2:1362203/150‑1 (MQ=255)
                                                                                tgatgaGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCa                                                                 >  1:570025/1‑150 (MQ=255)
                                                                                  atgaGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAgg                                                               <  2:1106284/150‑1 (MQ=255)
                                                                                  atgaGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAgg                                                               <  1:1442966/150‑1 (MQ=255)
                                                                                  atgaGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAgg                                                               <  1:511733/150‑1 (MQ=255)
                                                                                  atgaGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAgg                                                               >  1:1585680/1‑150 (MQ=255)
                                                                                    gagaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGcc                                                             >  2:1437453/1‑150 (MQ=255)
                                                                                         caatcaGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCCCGCACCTGTTGCAGGCCATCTa                                                        <  1:1014037/150‑1 (MQ=255)
                                                                                           atcaGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATg                                                      <  2:994222/150‑1 (MQ=255)
                                                                                            tcaGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGa                                                     <  1:1199845/150‑1 (MQ=255)
                                                                                             caGGCCGTTGCCAGCATGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGGTACTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGATTGTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCAATGaa                                                    >  2:1342612/1‑150 (MQ=255)
                                                                                              aGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAg                                                   >  2:1387041/1‑150 (MQ=255)
                                                                                                 ccGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAgggg                                                <  1:1144232/150‑1 (MQ=255)
                                                                                                  cGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGggt                                               <  1:345722/150‑1 (MQ=255)
                                                                                                  cGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGggt                                               <  2:1268751/150‑1 (MQ=255)
                                                                                                    ttGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGggtgg                                             <  2:982405/150‑1 (MQ=255)
                                                                                                      gCCAGCTTGCCGAAAGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGgtggtg                                           <  1:1017075/150‑1 (MQ=255)
                                                                                                              gCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCa                                   >  2:1542165/1‑150 (MQ=255)
                                                                                                              gCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCa                                   <  1:714388/150‑1 (MQ=255)
                                                                                                                  aagggggggggCGATCTCTTTTTCCTGCCGTTCGTGTACGGCAGCATCGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGGGGTGTTCAGCCATATg                               <  2:1450395/144‑1 (MQ=255)
                                                                                                                   aGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGa                              <  1:857559/150‑1 (MQ=255)
                                                                                                                         ggggCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCAcc                        >  2:1474221/1‑150 (MQ=255)
                                                                                                                         ggggCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCAcc                        <  2:671417/150‑1 (MQ=255)
                                                                                                                               aTCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAAcc                  <  1:834205/150‑1 (MQ=255)
                                                                                                                                 ctgtttttCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGa                <  1:152025/147‑1 (MQ=255)
                                                                                                                                  tctTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCata                                 <  2:435370/132‑1 (MQ=255)
                                                                                                                                  tctTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCata                                 >  1:435370/1‑132 (MQ=255)
                                                                                                                                     cgttgcTGCCGTACAGGAACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATgc            >  1:1377479/6‑150 (MQ=255)
                                                                                                                                        tgcTGCCGTACAGGAACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGcgcg         >  1:428382/3‑150 (MQ=255)
                                                                                                                                        tCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGcgcg         <  1:1387041/150‑1 (MQ=255)
                                                                                                                                         gcTGCCGTACAGGAACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGa        >  1:307167/2‑150 (MQ=255)
                                                                                                                                         ccTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGa        >  2:508763/1‑150 (MQ=255)
                                                                                                                                         ccTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGa        >  1:894522/1‑150 (MQ=255)
                                                                                                                                          cTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGaa       >  2:221668/1‑150 (MQ=255)
                                                                                                                                            gCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCGTATGACCCACCTCAACCGAATGCGCGAACg     <  1:1107947/150‑1 (MQ=255)
                                                                                                                                               gTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGttt  <  1:805704/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTT  >  NC_000913/3747963‑3748255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑