Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,852,559	T→A	6.3%	K76* (AAA→TAA) ‡	ilvB ←	acetohydroxy acid synthase I subunit IlvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,852,559	0	T	A	6.3%	240.9 / 3.5	75	K76* (AAA→TAA) ‡	ilvB	acetohydroxy acid synthase I subunit IlvB
Reads supporting (aligned to +/- strand):  ref base T (24/46);  new base A (3/2);  total (27/48)
Fisher's exact test for biased strand distribution p-value = 3.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATG  >  NC_000913/3852410‑3852704                                                                                                                                                      |                                                                                                                                                  cACTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGttt                                                                                                                                                   >  2:220072/1‑150 (MQ=255) cACTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGttt                                                                                                                                                   >  2:76717/1‑150 (MQ=255)   cTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTTCAGGCCATAAAGACCGGGGGTTTAc                                                                                                                                                 <  2:1363706/150‑1 (MQ=255)      ccTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGt                                                                                                                                              >  2:412293/1‑150 (MQ=255)       cTGGAAGGCGTCGGTGCCGATCATCGAGTCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTc                                                                                                                                             >  2:1399088/1‑150 (MQ=255)       cTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTc                                                                                                                                             <  2:144402/150‑1 (MQ=255)       cTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTc                                                                                                                                             <  1:1549390/150‑1 (MQ=255)         ggAAGGCGTCGGTGCCGAGCATCGAGGCGGGAACCTGACCAGGGATGCAAATCAGCGGGATGGAGGCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCgg                                                                                                                                           <  2:569849/150‑1 (MQ=255)          gAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGt                                                                                                                                          <  1:860537/150‑1 (MQ=255)          gAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGt                                                                                                                                          <  2:371726/150‑1 (MQ=255)           aaGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTg                                                                                                                                         <  1:792879/150‑1 (MQ=255)           aaGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGACGGTAAACCGGCGGtc                                                                                                                                         <  1:566219/150‑2 (MQ=255)             ggCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCACACCCGGTCCGCTACAGGCCATACAGACCGACGGTAAACCGGCGGtctg                                                                                                                                       <  1:1302529/150‑4 (MQ=255)                     tGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCGGCGCATCGGCAATGGCGGTCTCCAGGTTAGTCGCAGCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTc                                                                                                                               <  2:150795/150‑1 (MQ=255)                      gCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTcc                                                                                                                              <  2:1433622/150‑1 (MQ=255)                      gCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTcc                                                                                                                              >  1:886927/1‑150 (MQ=255)                             atcGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCg                                                                                                                       <  2:438431/150‑1 (MQ=255)                             atcGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCg                                                                                                                       <  1:1326349/150‑1 (MQ=255)                                gAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATa                                                                                                                    <  2:758540/150‑1 (MQ=255)                                gAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATa                                                                                                                    >  1:632102/1‑150 (MQ=255)                                  ggCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATaaa                                                                                                                  >  2:939179/1‑150 (MQ=255)                                   gCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAg                                                                                                                 <  1:475649/150‑1 (MQ=255)                                         aCCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCcgcg                                                                                                           <  1:1139616/150‑1 (MQ=255)                                            tGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGccc                                                                                                        <  2:264747/150‑1 (MQ=255)                                              aCCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTg                                                                                                      <  1:1223497/150‑1 (MQ=255)                                                  gTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCa                                                                                                  <  2:418436/150‑1 (MQ=255)                                                   tGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCCCCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCAt                                                                                                 >  2:1419842/1‑150 (MQ=255)                                                    gATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATg                                                                                                <  2:136244/150‑1 (MQ=255)                                                     aTGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGa                                                                                               <  2:1449378/150‑1 (MQ=255)                                                      tGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGAc                                                                                              >  2:1249648/1‑150 (MQ=255)                                                         aaaTCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACggg                                                                                           <  2:189010/150‑1 (MQ=255)                                                         aaaTCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACggg                                                                                           <  2:636076/150‑1 (MQ=255)                                                            tCAGCGGGATGGAGACCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCa                                                                                        <  2:528398/150‑1 (MQ=255)                                                             cAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAg                                                                                       >  1:898370/1‑150 (MQ=255)                                                              aGCGGGATGGAGGCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCGTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGa                                                                                      <  2:451858/150‑1 (MQ=255)                                                                    aTGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATgg                                                                                <  1:537759/150‑1 (MQ=255)                                                                     tGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGc                                                                               >  2:246314/1‑150 (MQ=255)                                                                      ggAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCg                                                                              <  2:92314/150‑1 (MQ=255)                                                                         gTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGAt                                                                           >  2:1058976/1‑150 (MQ=255)                                                                         gTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGAt                                                                           <  1:540979/150‑1 (MQ=255)                                                                          tCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGAtt                                                                          <  2:103676/150‑1 (MQ=255)                                                                           ccAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGAttt                                                                         >  2:1058688/1‑150 (MQ=255)                                                                            cAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTg                                                                        >  1:1599655/1‑150 (MQ=255)                                                                             aGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGc                                                                       >  1:57504/1‑150 (MQ=255)                                                                                 gcgcATCTGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGc                                                                   >  2:1158897/1‑150 (MQ=255)                                                                                  cgcATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCt                                                                  <  1:76717/150‑1 (MQ=255)                                                                                     aTCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTg                                                               <  2:898370/150‑1 (MQ=255)                                                                                      tCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTgg                                                              <  2:1316412/150‑1 (MQ=255)                                                                                      tCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTgg                                                              <  1:714646/150‑1 (MQ=255)                                                                                        ggCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCt                                                            <  1:443616/150‑1 (MQ=255)                                                                                          cAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGGTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTa                                                          >  2:1175118/1‑150 (MQ=255)                                                                                           aaTGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTaa                                                         <  2:632102/150‑1 (MQ=255)                                                                                             tGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAgg                                                       <  2:57504/150‑1 (MQ=255)                                                                                              ggCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGc                                                      >  2:1399225/1‑150 (MQ=255)                                                                                                cGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCAt                                                    <  2:2720/150‑1 (MQ=255)                                                                                                 ggTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCAGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATc                                                   >  1:797770/1‑150 (MQ=255)                                                                                                  gTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTa                                                          >  1:829591/1‑142 (MQ=255)                                                                                                  gTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTa                                                          <  2:829591/142‑1 (MQ=255)                                                                                                    cACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTa                                                >  2:1359117/1‑150 (MQ=255)                                                                                                        aGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAca                                            >  2:856496/1‑150 (MQ=255)                                                                                                             aGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcaggcag                                       <  1:1249648/150‑1 (MQ=255)                                                                                                              gTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAAcaggcagg                                      >  2:468498/1‑150 (MQ=255)                                                                                                               tCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCTTCGTAAACAGGCAGGa                                     <  2:1561391/150‑1 (MQ=255)                                                                                                                  cACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAg                                  <  1:372467/150‑1 (MQ=255)                                                                                                                  cACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAg                                  >  2:656899/1‑150 (MQ=255)                                                                                                                   aCCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGa                                 <  1:220072/150‑1 (MQ=255)                                                                                                                   aCCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGa                                 <  1:411430/150‑1 (MQ=255)                                                                                                                     ccGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAAc                               <  1:412293/150‑1 (MQ=255)                                                                                                                                  ggCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGcc                  >  1:198952/1‑150 (MQ=255)                                                                                                                                       tACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTca             <  1:662869/150‑1 (MQ=255)                                                                                                                                       tACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTca             <  1:387186/150‑1 (MQ=255)                                                                                                                                        aCAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGTATGCCTGTcac            <  2:386458/150‑1 (MQ=255)                                                                                                                                         cgcaCCGACGGTAAACCGGCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTcaca           >  2:278042/4‑150 (MQ=255)                                                                                                                                         cgcaCCGACGGTAAACCGGCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTcaca           >  1:626080/4‑150 (MQ=255)                                                                                                                                          aGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGGTAGAACCGCCCGGAATGCCTGTCACaa          >  1:712032/1‑150 (MQ=255)                                                                                                                                             ccgacgGTAAACCGGCGGTGCGCGCCATTCCCTGAGCGATAAAGCCAGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATATCACTGCTTTGGCTTAAGGCATCGTAAACAGGCAGCATAGAACCGCCCGGAGTGCCAGTCACAATCt       >  1:701065/1‑150 (MQ=255)                                                                                                                                                  ggTTTACCGTCGGTGCGCGCCTTTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATg  <  2:301349/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                  CACTTCCTGGAAGGCGTCGGTGCCGATCATCGAGGCGGGAACCTGACCAGTGATGCAAATCAGCGGGATGGAGTCCAGCCGCGCATCGGCAATGGCGGTCACCAGGTTAGTCGCACCCGGTCCGCTACAGGCCATACAGACCGCCGGTTTACCGTCGGTGCGCGCCATTCCCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATG  >  NC_000913/3852410‑3852704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑