Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,874,050 C→A 5.8% C142F (TGC→TTC) ‡ dgoK ← 2‑dehydro‑3‑deoxygalactonokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,874,0500CA 5.8% 290.9 / 5.3 86C142F (TGC→TTC) ‡dgoK2‑dehydro‑3‑deoxygalactonokinase
Reads supporting (aligned to +/- strand):  ref base C (38/43);  new base A (2/3);  total (40/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

CAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACTGACGTT  >  NC_000913/3873901‑3874199
                                                                                                                                                     |                                                                                                                                                     
cAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGc                                                                                                                                                       <  1:285258/150‑1 (MQ=255)
     ccAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGAGTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATgg                                                                                                                                                  <  2:1299850/150‑1 (MQ=255)
      cAGCTGTGAAGGCATCCGCAGAGTTTTCCTGCGGCGTCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATggg                                                                                                                                                 <  2:819247/150‑1 (MQ=255)
         cTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTGCAAATGGGtgc                                                                                                                                              <  1:1599136/150‑3 (MQ=255)
          tgtgAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTcc                                                                                                                                             >  1:164764/1‑150 (MQ=255)
          tgtgAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTGCAAATGGGtgca                                                                                                                                             <  1:1339219/150‑4 (MQ=255)
           gtgAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCg                                                                                                                                            >  1:1246350/1‑150 (MQ=255)
             gAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCggg                                                                                                                                          <  1:1002607/150‑1 (MQ=255)
             gAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCggg                                                                                                                                          >  2:584817/1‑150 (MQ=255)
             gAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCggg                                                                                                                                          >  2:1101524/1‑150 (MQ=255)
             gAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCggg                                                                                                                                          <  1:1347492/150‑1 (MQ=255)
             gAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTGCAAATGGGtgcaggc                                                                                                                                          <  1:1021934/150‑7 (MQ=255)
                 gCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATg                                                                                                                                      >  2:510747/1‑150 (MQ=255)
                  cATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGa                                                                                                                                     >  2:29773/1‑150 (MQ=255)
                  cATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGa                                                                                                                                     <  1:968772/150‑1 (MQ=255)
                      ggCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATATGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATa                                                                                                                                 <  2:1457905/150‑1 (MQ=255)
                            gTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAagaga                                                                                                                           <  1:827794/150‑1 (MQ=255)
                               ttCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGagga                                                                                                                        <  1:471493/150‑1 (MQ=255)
                                tCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGaggaa                                                                                                                       >  1:4238/1‑150 (MQ=255)
                                 ccTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGaggaag                                                                                                                      >  1:968039/1‑150 (MQ=255)
                                  cTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGaggaagg                                                                                                                     >  1:117938/1‑150 (MQ=255)
                                  cTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCAACCATTTGCAATGGGTTCCGGGCATGACATAAAGAGaggaagg                                                                                                                     <  2:1434570/150‑1 (MQ=255)
                                   tGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGaggaagga                                                                                                                    >  2:300921/1‑150 (MQ=255)
                                      ggcggcAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGcc                                                                                                                 <  1:547671/150‑1 (MQ=255)
                                         ggcAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCaga                                                                                                              <  1:412441/150‑1 (MQ=255)
                                                cTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTcgcg                                                                                                       <  1:867715/150‑1 (MQ=255)
                                                 tGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGGTcgcgc                                                                                                      <  2:1371713/150‑1 (MQ=255)
                                                 tGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTcgcgc                                                                                                      >  1:1582321/1‑150 (MQ=255)
                                                  gcgcCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTcgcgcg                                                                                                     >  1:1547539/1‑150 (MQ=255)
                                                       aatcaatGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGAt                                                                                                >  1:431394/1‑150 (MQ=255)
                                                        atcaatGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATc                                                                                               >  1:1288364/1‑150 (MQ=255)
                                                         tcaatGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCa                                                                                              >  2:650628/1‑150 (MQ=255)
                                                           aatGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAAt                                                                                            >  1:944645/1‑150 (MQ=255)
                                                              gAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGa                                                                                                                                     <  1:111753/106‑1 (MQ=255)
                                                              gAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGa                                                                                                                                     >  2:111753/1‑106 (MQ=255)
                                                              gAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATtgt                                                                                         >  1:1365071/1‑150 (MQ=255)
                                                               aGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATtgtg                                                                                        <  2:52028/150‑1 (MQ=255)
                                                                gTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTgtgt                                                                                       <  2:431394/150‑1 (MQ=255)
                                                                     ttAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTttctt                                                                                  <  1:1101524/150‑1 (MQ=255)
                                                                      tAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTttcttc                                                                                 <  1:764975/150‑1 (MQ=255)
                                                                       aaCAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTcg                                                                                <  1:218364/150‑1 (MQ=255)
                                                                        aCAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTcgc                                                                               >  2:608764/1‑150 (MQ=255)
                                                                         cAGTAACTGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCAGGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTcgcc                                                                              <  1:1457964/150‑1 (MQ=255)
                                                                         cAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTcgcc                                                                              >  2:1355729/1‑150 (MQ=255)
                                                                         cAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTcgcc                                                                              >  2:1090021/1‑150 (MQ=255)
                                                                         cAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTcgcc                                                                              >  1:198559/1‑150 (MQ=255)
                                                                          aGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTcgccg                                                                             >  2:1044432/1‑150 (MQ=255)
                                                                          aGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTcgccg                                                                             <  1:1404171/150‑1 (MQ=255)
                                                                          aGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGATCGCGAGCCGATCAATTGTGTTTCTTcgcag                                                                             >  2:562101/1‑148 (MQ=255)
                                                                           gTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTcgccgc                                                                            >  2:1327105/1‑150 (MQ=255)
                                                                           gTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTcgccgc                                                                            <  2:968039/150‑1 (MQ=255)
                                                                             aaaTGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCgcgc                                                                          <  2:495614/150‑1 (MQ=255)
                                                                                tgatgTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATc                                                                       <  2:567888/150‑1 (MQ=255)
                                                                                  atgTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATcac                                                                     >  1:736303/1‑150 (MQ=255)
                                                                                        ttCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATtgtg                                                               <  1:510747/150‑1 (MQ=255)
                                                                                         tCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTgg                                                              <  1:902473/150‑1 (MQ=255)
                                                                                           aCCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGtt                                                            >  1:756404/1‑150 (MQ=255)
                                                                                              ggTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATc                                                         <  2:1582321/150‑1 (MQ=255)
                                                                                               gTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCg                                                        <  1:1044432/150‑1 (MQ=255)
                                                                                                tcatcaCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGt                                                       <  2:1365071/150‑1 (MQ=255)
                                                                                                   tcaCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCAt                                                    >  2:261064/1‑150 (MQ=255)
                                                                                                    caCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATg                                                   <  1:608764/150‑1 (MQ=255)
                                                                                                        gTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATgagag                                               <  2:1617945/150‑1 (MQ=255)
                                                                                                            gAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGacac                                           >  2:1288936/1‑150 (MQ=255)
                                                                                                                      gATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATgag                                                 >  1:1146015/1‑134 (MQ=255)
                                                                                                                      gATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATgag                                                 <  2:1146015/134‑1 (MQ=255)
                                                                                                                      gATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCggg                                 <  2:944645/150‑1 (MQ=255)
                                                                                                                           gCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGaataa                            >  1:546864/1‑150 (MQ=255)
                                                                                                                           gCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGaataa                            >  2:1529053/1‑150 (MQ=255)
                                                                                                                              ggCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATcc                         <  2:736303/150‑1 (MQ=255)
                                                                                                                                  aTCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGat                     <  2:1288364/150‑1 (MQ=255)
                                                                                                                                   tCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGCGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACAAATAATCCGGGAATAATCCAGata                    >  1:1060136/1‑150 (MQ=255)
                                                                                                                                   tCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGata                    <  1:1029775/150‑1 (MQ=255)
                                                                                                                                    cGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGatat                   >  1:175675/1‑150 (MQ=255)
                                                                                                                                      cccggaaCCCATTGCAAATGGGTTCCGGGCATGACATAAAGATAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTg                 >  2:45488/7‑150 (MQ=255)
                                                                                                                                         tGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGaataa                            >  1:923694/1‑136 (MQ=255)
                                                                                                                                         tGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGaataa                            <  2:923694/136‑1 (MQ=255)
                                                                                                                                         tGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCg              <  2:1246350/150‑1 (MQ=255)
                                                                                                                                         tGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCg              >  2:189287/1‑150 (MQ=255)
                                                                                                                                          gaaCCCATTGCAAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGc             >  1:1099336/3‑150 (MQ=255)
                                                                                                                                          gCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGc             <  1:536676/150‑1 (MQ=255)
                                                                                                                                             cccATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCaa          >  1:1303023/1‑150 (MQ=255)
                                                                                                                                               cATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACt        >  1:1522298/1‑150 (MQ=255)
                                                                                                                                                 tttGCAATGGGTTCCGGGCATGACATAAAGCGAGGCAGGAGCCAGAGCTCGCGCGCCGAGCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACTGa      <  2:1051293/150‑1 (MQ=255)
                                                                                                                                                   tGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACTGACg    <  1:1466873/150‑1 (MQ=255)
                                                                                                                                                   tGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACTGACg    <  2:1352360/150‑1 (MQ=255)
                                                                                                                                                    gCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACTGACGt   <  2:929613/150‑1 (MQ=255)
                                                                                                                                                     cAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACTGACGtt  >  2:756215/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAACTGACGTT  >  NC_000913/3873901‑3874199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑