Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,095,837 T→G 8.5% E49A (GAA→GCA) ‡ rhaA ← L‑rhamnose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,095,8370TG 8.5% 276.7 / 16.3 94E49A (GAA→GCA) ‡rhaAL‑rhamnose isomerase
Reads supporting (aligned to +/- strand):  ref base T (41/45);  new base G (5/3);  total (46/48)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

GATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGC  >  NC_000913/4095688‑4095986
                                                                                                                                                     |                                                                                                                                                     
gatagatGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGtttt                                                                                                                                                       <  2:769870/150‑1 (MQ=255)
      tGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAAc                                                                                                                                                 >  2:137371/1‑150 (MQ=255)
      tGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAAc                                                                                                                                                 >  1:700461/1‑150 (MQ=255)
         cATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCgg                                                                                                                                              >  1:1029828/1‑150 (MQ=255)
         cATGTAAATTAAGCCGTTTCGGCCCCGGAAGCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCgg                                                                                                                                              <  2:867769/150‑1 (MQ=255)
           tGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGaa                                                                                                                                            <  2:618628/150‑1 (MQ=255)
           tGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGaa                                                                                                                                            >  2:306046/1‑150 (MQ=255)
            gTAAATTAAGCGGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACTTAGCTCACTGGCATTAGGCGCTTTTCCCGGATAATTGCCTGTGGCCTGAATCCCCGCTTTCAGCGAACCTTCCGGTTTTGAAAACCCGGaag                                                                                                                                           <  2:771673/150‑2 (MQ=255)
            gTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGaaa                                                                                                                                           <  1:1453273/150‑1 (MQ=255)
            gTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGaaa                                                                                                                                           <  1:1241786/150‑1 (MQ=255)
            gTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTCTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGaaa                                                                                                                                           >  1:1064681/1‑150 (MQ=255)
             tAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAAc                                                                                                                                          >  1:1591498/1‑150 (MQ=255)
                aTTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAAcatc                                                                                                                                       <  1:1501635/150‑1 (MQ=255)
                     gCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGc                                                                                                                                  >  2:837422/1‑150 (MQ=255)
                     gCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGc                                                                                                                                  <  2:399384/150‑1 (MQ=255)
                          ttCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGc                                                                                                                             >  2:79621/1‑150 (MQ=255)
                               ccccGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCcagca                                                                                                                        <  2:1237495/150‑1 (MQ=255)
                                 ccGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGt                                                                                                                      <  1:907055/150‑1 (MQ=255)
                                  cGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTg                                                                                                                     >  1:648422/1‑150 (MQ=255)
                                  cGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTg                                                                                                                     >  1:29360/1‑150 (MQ=255)
                                     aaTCAGCCGCATTGCCTGTTCCAGTTCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCAt                                                                                                                  <  2:492629/150‑1 (MQ=255)
                                         aGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGaa                                                                                                              <  2:1235395/150‑1 (MQ=255)
                                          gCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGaaa                                                                                                             >  1:947516/1‑150 (MQ=255)
                                            cGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACg                                                                                                           <  2:477207/150‑1 (MQ=255)
                                               aTAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGt                                                                                                        <  1:930018/150‑1 (MQ=255)
                                               aTAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGt                                                                                                        <  1:359261/150‑1 (MQ=255)
                                                  gCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCTTCGCCCTGCCAGCAGTGCATTGAAACGGGTaaa                                                                                                     >  2:1564595/1‑150 (MQ=255)
                                                   ccTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAAc                                                                                                    <  1:1449851/150‑1 (MQ=255)
                                                      gTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGAt                                                                                                 >  2:1601896/1‑150 (MQ=255)
                                                      gTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGAt                                                                                                 <  1:79621/150‑1 (MQ=255)
                                                        tCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCa                                                                                               <  2:993368/150‑1 (MQ=255)
                                                        tCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCa                                                                                               <  2:907731/150‑1 (MQ=255)
                                                         ccAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGTTTTGAAAACCCGGa                                                                                                                                             >  1:1241655/1‑103 (MQ=255)
                                                         ccAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGTTTTGAAAACCCGGa                                                                                                                                             <  2:1235892/103‑1 (MQ=255)
                                                         ccAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGTTTTGAAAACCCGGa                                                                                                                                             >  1:1235892/1‑103 (MQ=255)
                                                         ccAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGTTTTGAAAACCCGGa                                                                                                                                             <  2:1241655/103‑1 (MQ=255)
                                                         ccAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCaa                                                                                              <  1:821967/150‑1 (MQ=255)
                                                          cAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAg                                                                                             <  2:714717/150‑1 (MQ=255)
                                                                ggCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGgcg                                                                                       >  1:589588/1‑150 (MQ=255)
                                                                  cACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCa                                                                                     >  1:1426928/1‑150 (MQ=255)
                                                                          tCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGcctcc                                                                             <  2:401699/150‑1 (MQ=255)
                                                                          tCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGcctcc                                                                             <  1:773282/150‑1 (MQ=255)
                                                                           cACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGgc                                                                                        >  2:306714/1‑138 (MQ=255)
                                                                           cACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGgc                                                                                        <  1:306714/138‑1 (MQ=255)
                                                                           cACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGcctcct                                                                            >  1:1105936/1‑150 (MQ=255)
                                                                           cACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGcctcct                                                                            >  1:726345/1‑150 (MQ=255)
                                                                             cTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCg                                                                          <  2:68894/150‑1 (MQ=255)
                                                                               ggCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGAc                                                                        <  2:589588/150‑1 (MQ=255)
                                                                                gCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACa                                                                       >  2:1477616/1‑150 (MQ=255)
                                                                                 cATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACAt                                                                      >  2:232527/1‑150 (MQ=255)
                                                                                  aTTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTACATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATc                                                                     <  1:44619/150‑1 (MQ=255)
                                                                                   ttACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCa                                                                    >  2:958265/1‑150 (MQ=255)
                                                                                      cgcgCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGcctcc                                                                             <  2:1500100/138‑1 (MQ=255)
                                                                                      cgcgCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGcctcc                                                                             >  1:1500100/1‑138 (MQ=255)
                                                                                       gcgcTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATcc                                                                <  1:1471383/150‑1 (MQ=255)
                                                                                         gcTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATcccc                                                              <  2:294852/150‑1 (MQ=255)
                                                                                            ttGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCAcc                                                           <  2:996805/150‑1 (MQ=255)
                                                                                            ttGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCAcc                                                           <  1:1535149/150‑1 (MQ=255)
                                                                                               cccGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGgc                                                                                        <  1:223188/118‑1 (MQ=255)
                                                                                               cccGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGgc                                                                                        >  2:223188/1‑118 (MQ=255)
                                                                                               cccGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCAccgcc                                                        >  2:1550182/1‑150 (MQ=255)
                                                                                                 cGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACcgccgc                                                      >  1:1532112/1‑150 (MQ=255)
                                                                                                 cGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACcgccgc                                                      >  1:711884/1‑150 (MQ=255)
                                                                                                    aTAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGaa                                                   <  1:1174548/150‑1 (MQ=255)
                                                                                                       aTTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACg                                                <  1:1041530/150‑1 (MQ=255)
                                                                                                           ccTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGt                                            >  1:991719/1‑150 (MQ=255)
                                                                                                              gtgGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTc                                         <  1:137371/150‑1 (MQ=255)
                                                                                                                ggCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGc                                       >  2:1456898/1‑150 (MQ=255)
                                                                                                                 gCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCt                                      <  1:306046/150‑1 (MQ=255)
                                                                                                                  ccTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTa                                     >  2:1331808/1‑150 (MQ=255)
                                                                                                                  ccTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACAGCGTTTCGcgg                                     >  2:821493/1‑148 (MQ=255)
                                                                                                                  ccTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACAGCGTTTCGcgg                                     <  1:821493/150‑3 (MQ=255)
                                                                                                                   cTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATc                                                                     <  2:71153/117‑1 (MQ=255)
                                                                                                                   cTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATc                                                                     >  1:71153/1‑117 (MQ=255)
                                                                                                                    tGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACg                                                >  2:639139/1‑137 (MQ=255)
                                                                                                                    tGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACg                                                <  1:639139/137‑1 (MQ=255)
                                                                                                                     gAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCt                                  >  1:1323694/1‑150 (MQ=255)
                                                                                                                     gAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACAGCGTTTCGcggcgg                                  <  2:319304/150‑6 (MQ=255)
                                                                                                                     gAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACAGCGTTTCGcggcgg                                  <  2:700461/150‑6 (MQ=255)
                                                                                                                       aTCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTcc                                >  1:829946/1‑150 (MQ=255)
                                                                                                                         ccccccGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCa                              >  1:888418/1‑150 (MQ=255)
                                                                                                                          cccccGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAg                             >  2:1375365/1‑150 (MQ=255)
                                                                                                                           ccccGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAgg                            <  2:308770/150‑1 (MQ=255)
                                                                                                                              cGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACg                                                <  1:378378/127‑1 (MQ=255)
                                                                                                                              cGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACg                                                >  2:378378/1‑127 (MQ=255)
                                                                                                                                   aGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTcc                    >  2:622211/1‑150 (MQ=255)
                                                                                                                                     cGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAg                  <  1:1601896/150‑1 (MQ=255)
                                                                                                                                      gAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGt                 >  2:443899/1‑150 (MQ=255)
                                                                                                                                       aaCCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGtt                <  1:1008095/150‑1 (MQ=255)
                                                                                                                                        tgtttCCGGTTTTGAAAACCCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTg               >  1:1062330/4‑150 (MQ=255)
                                                                                                                                        tgtttCCGGTTTTGAAAACCCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTg               >  1:1024876/4‑150 (MQ=255)
                                                                                                                                         gtttCCGGTTTTGAAAACCCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGa              >  1:837361/3‑150 (MQ=255)
                                                                                                                                          cTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAg             >  1:1109530/1‑150 (MQ=255)
                                                                                                                                               gggTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTc        <  1:232527/150‑1 (MQ=255)
                                                                                                                                                ggTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCa       >  1:603463/1‑150 (MQ=255)
                                                                                                                                                     tCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATgcgc  >  1:1253943/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGC  >  NC_000913/4095688‑4095986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑