Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,095,942 T→C 9.4% Q14R (CAG→CGG) ‡ rhaA ← L‑rhamnose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,095,9420TC 9.4% 271.1 / 15.3 96Q14R (CAG→CGG) ‡rhaAL‑rhamnose isomerase
Reads supporting (aligned to +/- strand):  ref base T (43/44);  new base C (6/3);  total (49/47)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01

CCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCGGTGGTGCTGAC  >  NC_000913/4095795‑4096089
                                                                                                                                                   |                                                                                                                                                   
ccTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGt                                                                                                                                                   >  1:991719/1‑150 (MQ=255)
   gtgGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTc                                                                                                                                                <  1:137371/150‑1 (MQ=255)
     ggCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGc                                                                                                                                              >  2:1456898/1‑150 (MQ=255)
      gCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCt                                                                                                                                             <  1:306046/150‑1 (MQ=255)
       ccTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTa                                                                                                                                            >  2:1331808/1‑150 (MQ=255)
       ccTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACAGCGTTTCGcgg                                                                                                                                            >  2:821493/1‑148 (MQ=255)
       ccTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACAGCGTTTCGcgg                                                                                                                                            <  1:821493/150‑3 (MQ=255)
          gAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCt                                                                                                                                         >  1:1323694/1‑150 (MQ=255)
          gAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACAGCGTTTCGcggcgg                                                                                                                                         <  2:700461/150‑6 (MQ=255)
          gAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACAGCGTTTCGcggcgg                                                                                                                                         <  2:319304/150‑6 (MQ=255)
            aTCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTcc                                                                                                                                       >  1:829946/1‑150 (MQ=255)
              ccccccGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCa                                                                                                                                     >  1:888418/1‑150 (MQ=255)
               cccccGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAg                                                                                                                                    >  2:1375365/1‑150 (MQ=255)
                ccccGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAgg                                                                                                                                   <  2:308770/150‑1 (MQ=255)
                        aGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTcc                                                                                                                           >  2:622211/1‑150 (MQ=255)
                          cGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAg                                                                                                                         <  1:1601896/150‑1 (MQ=255)
                           gAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGt                                                                                                                        >  2:443899/1‑150 (MQ=255)
                            aaCCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGtt                                                                                                                       <  1:1008095/150‑1 (MQ=255)
                             tgtttCCGGTTTTGAAAACCCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTg                                                                                                                      >  1:1024876/4‑150 (MQ=255)
                             tgtttCCGGTTTTGAAAACCCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTg                                                                                                                      >  1:1062330/4‑150 (MQ=255)
                              gtttCCGGTTTTGAAAACCCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGa                                                                                                                     >  1:837361/3‑150 (MQ=255)
                               cTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAg                                                                                                                    >  1:1109530/1‑150 (MQ=255)
                                    gggTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTc                                                                                                               <  1:232527/150‑1 (MQ=255)
                                     ggTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCa                                                                                                              >  1:603463/1‑150 (MQ=255)
                                          tCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATgcgc                                                                                                         >  1:1253943/1‑150 (MQ=255)
                                           cAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTNGNCATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATgcgcn                                                                                                        <  2:726345/150‑2 (MQ=255)
                                            aaaaCCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCaa                                                                                                       >  2:1424431/1‑150 (MQ=255)
                                            aaaaCCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCaa                                                                                                       >  2:997420/1‑150 (MQ=255)
                                            aaaaCCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCAAGTTGAGTGGTCATGCGCaa                                                                                                       >  2:743626/1‑150 (MQ=255)
                                               aCCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGc                                                                                                    <  1:1477616/150‑1 (MQ=255)
                                                  ggAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCGCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCGcc                                                                                                 <  2:1468048/150‑1 (MQ=255)
                                                    aaaCATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCtt                                                                                               <  1:873067/150‑1 (MQ=255)
                                                      aCATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTtg                                                                                             <  1:622211/150‑1 (MQ=255)
                                                      aCATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTtg                                                                                             <  1:1331808/150‑1 (MQ=255)
                                                      aCATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTtg                                                                                             <  2:1029828/150‑1 (MQ=255)
                                                       catcatCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTtgt                                                                                            >  1:1513661/1‑150 (MQ=255)
                                                       catcatCGCCCTGCCAGCAGTACATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTtgt                                                                                            >  2:861962/1‑150 (MQ=255)
                                                          catcGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGTGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTtgtctg                                                                                         >  2:971218/1‑150 (MQ=255)
                                                          catcGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTtgtctg                                                                                         <  1:1456898/150‑1 (MQ=255)
                                                          catcGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTtgtctg                                                                                         >  2:1392150/1‑150 (MQ=255)
                                                          catcGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTtgtctg                                                                                         >  2:310164/1‑150 (MQ=255)
                                                               cccTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCgtg                                                                                    <  2:991719/150‑1 (MQ=255)
                                                                ccTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCgtgt                                                                                   <  2:888418/150‑1 (MQ=255)
                                                                  tGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTag                                                                                 >  2:510555/1‑150 (MQ=255)
                                                                   gCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTaga                                                                                <  2:947516/150‑1 (MQ=255)
                                                                       gcagTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGa                                                                            >  2:863292/1‑150 (MQ=255)
                                                                            gCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTg                                                                       >  1:665791/1‑150 (MQ=255)
                                                                              aTTGGAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGATCCTTTGTCTGTCGTGTAGAGTGAATCTgcg                                                                     >  2:864308/1‑150 (MQ=255)
                                                                               ttGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTgcgc                                                                    <  2:1591498/150‑1 (MQ=255)
                                                                                tGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGcc                                                                   <  2:711884/150‑1 (MQ=255)
                                                                                 gAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCca                                                                  >  2:417210/1‑150 (MQ=255)
                                                                                        gTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTg                                                           <  1:997420/150‑1 (MQ=255)
                                                                                         tAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTgg                                                          >  2:1576500/1‑150 (MQ=255)
                                                                                          aaaCGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTggg                                                         <  1:596120/150‑1 (MQ=255)
                                                                                             cGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCaa                                                      >  1:73368/1‑150 (MQ=255)
                                                                                                 cAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTc                                                  <  2:648422/150‑1 (MQ=255)
                                                                                                 cAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTc                                                  <  2:29360/150‑1 (MQ=255)
                                                                                                  aaGTTGGCGCAGTGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCa                                                 <  1:971218/150‑1 (MQ=255)
                                                                                                  aaGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCa                                                 <  1:310164/150‑1 (MQ=255)
                                                                                                   aGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGt                                                                              <  1:1502378/120‑1 (MQ=255)
                                                                                                   aGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGt                                                                              >  2:1502378/1‑120 (MQ=255)
                                                                                                   aGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCAc                                                <  2:829946/150‑1 (MQ=255)
                                                                                                   aGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCAc                                                <  1:417210/150‑1 (MQ=255)
                                                                                                           cAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGa                                        <  1:743626/150‑1 (MQ=255)
                                                                                                             gcgcCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGAtt                                      >  1:592957/1‑150 (MQ=255)
                                                                                                             gcgcCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGAtt                                      <  2:1105936/150‑1 (MQ=255)
                                                                                                                cctcctCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGTCCTGTTCCCGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTTCGCAACATAGTGGGCAATTTCACTGTCAGGATTAgg                                   <  2:30757/150‑1 (MQ=255)
                                                                                                                cctcctCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAgg                                   >  2:221798/1‑150 (MQ=255)
                                                                                                                 ctcctcGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAggg                                  <  2:874068/150‑1 (MQ=255)
                                                                                                                 ctcctcGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAggg                                  >  1:160031/1‑150 (MQ=255)
                                                                                                                  tcctcGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAgggg                                 <  2:1109530/150‑1 (MQ=255)
                                                                                                                  tcctcGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAgggg                                 <  1:1564595/150‑1 (MQ=255)
                                                                                                                  tcctcGACATAAATCCCCACCGCCGCGAAACTCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAgggg                                 <  2:1426928/150‑1 (MQ=255)
                                                                                                                    ctcGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTa                               >  1:1303207/1‑150 (MQ=255)
                                                                                                                    ctcGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTa                               <  1:1392150/150‑1 (MQ=255)
                                                                                                                    ctcGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTa                               <  2:73368/150‑1 (MQ=255)
                                                                                                                    ctcGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTa                               >  2:705452/1‑150 (MQ=255)
                                                                                                                       gACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAACGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAg                            >  1:865114/1‑150 (MQ=255)
                                                                                                                         cATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAggt                          <  1:443899/150‑1 (MQ=255)
                                                                                                                           tCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAggtgg                        >  1:871873/1‑150 (MQ=255)
                                                                                                                              aTCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCa                     >  1:960878/1‑150 (MQ=255)
                                                                                                                                 cccACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGAt                  <  2:1323694/150‑1 (MQ=255)
                                                                                                                                  ccACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGAtt                 >  2:210261/1‑150 (MQ=255)
                                                                                                                                   cACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTc                >  2:1211929/1‑150 (MQ=255)
                                                                                                                                      gctagcgAAACAGCGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCATATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTcgcg             >  2:1172556/5‑150 (MQ=255)
                                                                                                                                      gctagcgAAACAGCGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTcgcg             >  1:157740/5‑150 (MQ=255)
                                                                                                                                       ctagcgAAACAGCGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCgg            >  2:954256/4‑150 (MQ=255)
                                                                                                                                        tagcgAAACAGCGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCggt           >  1:1228420/3‑150 (MQ=255)
                                                                                                                                         cgcgAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCggtg          <  1:705452/150‑1 (MQ=255)
                                                                                                                                         agcgAAACAGCGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCggtg          >  1:327216/2‑150 (MQ=255)
                                                                                                                                          gcgAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCggtgg         >  2:208352/1‑150 (MQ=255)
                                                                                                                                             aaaCGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCGGTGGTGc      <  1:1375365/150‑1 (MQ=255)
                                                                                                                                              aaCGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCGGTGGTGCt     <  2:603463/150‑1 (MQ=255)
                                                                                                                                                cGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCGGTGGTGCTga   <  2:1024876/150‑1 (MQ=255)
                                                                                                                                                cGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGCCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCGGTGGTGCTga   <  1:1550182/150‑1 (MQ=255)
                                                                                                                                                 gCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCGGTGGTGCTgac  >  1:879870/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCTGTGGCCTGAATCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAACGCTGTTTCGCTAGCTCCCAGGCCTGTTCCAGTTGAGTGGTCATGCGCAAAGCTCCTTTGTCTGTCGTGTAGAGTGAATCTGCGCCACATAGTGGGCAATTTCACTGTCAGGATTAGGGGTAAAGGTGGTCAGATTCGCGGTGGTGCTGAC  >  NC_000913/4095795‑4096089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑