BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,116,282	A→G	8.0%	I314T (ATT→ACT)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,282	0	A	G	8.0%	292.4 / 11.7	100	I314T (ATT→ACT)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base A (37/55); new base G (4/4); total (41/59)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 101 total aligned reads displayed.

CCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGG  >  NC_000913/4116133‑4116429

ccccGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGaa                                                                                                                                                     <  1:672907/150‑1 (MQ=255)

  ccGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATg                                                                                                                                                   <  2:952906/150‑1 (MQ=255)

   cgcgcgCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTGATTTCATCGCGCAGCCACTGAATgg                                                                                                                                                  <  2:1605428/150‑1 (MQ=255)

          aTACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGcc                                                                                                                                           <  2:1091449/150‑1 (MQ=255)

           tACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCt                                                                                                                                          >  2:734788/1‑150 (MQ=255)

           tACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCt                                                                                                                                          >  2:704548/1‑150 (MQ=255)

             cGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGc                                                                                                                                        >  2:534452/1‑150 (MQ=255)

             cGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCACATCCATTCAGTGGCTgcgcgatg                                                                                                                                        <  1:272919/150‑5 (MQ=255)

               ggTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCa                                                                                                                                      <  1:197495/150‑1 (MQ=255)

               ggTCACAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCa                                                                                                                                      >  2:1179528/1‑150 (MQ=255)

                gTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCATCCATTCAGTGGCTgcgcgatgaaa                                                                                                                                     <  2:1225808/150‑8 (MQ=255)

                gTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCATCCATTCAGTGGCTgcgcgatgaaa                                                                                                                                     <  1:1340086/150‑8 (MQ=255)

                gTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCATCCATTCAGTGGCTgcgcgatgaaa                                                                                                                                     <  1:1491983/150‑8 (MQ=255)

                  cccAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATaa                                                                                                                                   >  1:112050/1‑150 (MQ=255)

                  cccAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATaa                                                                                                                                   >  1:77229/1‑150 (MQ=255)

                        aCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCg                                                                                                                             <  1:802616/150‑1 (MQ=255)

                         cGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGc                                                                                                                            <  2:669908/150‑1 (MQ=255)

                         cGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGc                                                                                                                            <  2:283444/150‑1 (MQ=255)

                          ggCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGcc                                                                                                                           >  1:419449/1‑149 (MQ=255)

                           gcgcACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCAc                                                                                                                          <  1:1197865/150‑1 (MQ=255)

                               aCCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTc                                                                                                                      >  2:228229/1‑150 (MQ=255)

                                cccATCCCGGTAAATGCCGGAACCACATACACACCCTTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGACTCGTAGGCGTCGTTAATCAACTTCATTTCCTCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTcc                                                                                                                     <  2:1085171/150‑1 (MQ=255)

                                cccAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTcc                                                                                                                     <  1:734788/150‑1 (MQ=255)

                                 ccAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTc                                                                                                                      <  1:1135218/148‑1 (MQ=255)

                                 ccAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTc                                                                                                                      >  2:1135218/1‑148 (MQ=255)

                                 ccAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTc                                                                                                                      >  2:1140840/1‑148 (MQ=255)

                                 ccAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTc                                                                                                                      <  1:1140840/148‑1 (MQ=255)

                                    gCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTTGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACg                                                                                                                 <  1:1025111/150‑1 (MQ=255)

                                      ccGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCa                                                                                                               >  1:838651/1‑150 (MQ=255)

                                            aaTGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTc                                                                                                         >  1:1014287/1‑150 (MQ=255)

                                            aaTGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTc                                                                                                         <  2:486573/150‑1 (MQ=255)

                                             aTGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCa                                                                                                        >  1:245966/1‑150 (MQ=255)

                                             aTGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCa                                                                                                        <  1:1505622/150‑1 (MQ=255)

                                               gCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACt                                                                                                      >  1:605284/1‑150 (MQ=255)

                                                          aTACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATGCGTAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCgg                                                                                           <  2:434709/150‑1 (MQ=255)

                                                            acacacCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCTACGCATAGTTCACTTCGCCAGTCGGGc                                                                                         <  1:624459/150‑1 (MQ=255)

                                                               cacCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGc                                                                                      <  2:567433/150‑1 (MQ=255)

                                                               cacCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGc                                                                                      >  2:298041/1‑150 (MQ=255)

                                                                acCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCa                                                                                     <  1:1576945/150‑1 (MQ=255)

                                                                       gtgtTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGAtg                                                                              <  1:114424/150‑1 (MQ=255)

                                                                        ggtTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGGCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGAtgg                                                                             <  1:1594057/149‑1 (MQ=255)

                                                                         gtTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGAtggt                                                                            <  1:1426505/150‑1 (MQ=255)

                                                                          ttttGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGAtggtg                                                                           >  1:443724/1‑150 (MQ=255)

                                                                          ttttGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGAtggtg                                                                           <  1:1492880/150‑1 (MQ=255)

                                                                           tttGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGAtggtgg                                                                          <  2:1014287/150‑1 (MQ=255)

                                                                           tttGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGAtggtgg                                                                          <  1:941880/150‑1 (MQ=255)

                                                                            ttGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATggtggt                                                                         <  1:562057/150‑1 (MQ=255)

                                                                              gCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTca                                                                       <  2:1112289/150‑1 (MQ=255)

                                                                                 cTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCGTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCCCAGGCGATGGTGGTcagcc                                                                    >  2:846078/1‑149 (MQ=255)

                                                                                 cTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTcagca                                                                    >  1:642974/1‑150 (MQ=255)

                                                                                 cTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTcagca                                                                    >  2:1553419/1‑150 (MQ=255)

                                                                                 cTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTcagca                                                                    >  2:1239243/1‑150 (MQ=255)

                                                                                          cGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGtttt                                                           <  1:84054/150‑1 (MQ=255)

                                                                                          cGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGtttt                                                           >  1:882367/1‑150 (MQ=255)

                                                                                            aaaTATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCt                                                         <  1:1179528/150‑1 (MQ=255)

                                                                                            aaaTATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCt                                                         <  2:975565/150‑1 (MQ=255)

                                                                                            aaaTATCCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCt                                                         <  2:608005/150‑1 (MQ=255)

                                                                                             aaTATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTg                                                        <  2:1059592/150‑1 (MQ=255)

                                                                                               tatTCGGATTCGTAGGCGTGGTTAATCATCTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCTACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGAt                                                      <  2:1454098/150‑1 (MQ=255)

                                                                                               tatTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGAt                                                      >  2:1445729/1‑150 (MQ=255)

                                                                                                 ttCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGAttt                                                    >  2:1436391/1‑150 (MQ=255)

                                                                                                  tCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTc                                                   >  2:132258/1‑150 (MQ=255)

                                                                                                   cGGAATCGTAGGCGTCGTTAATCAACTTCATTTCCTCGCGCAGCCACTGAAAGGATGCGCCTGCCATAACCACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGGCCATTTCTGATTTCa                                                  >  2:378933/1‑150 (MQ=255)

                                                                                                     ccaTCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCAcc                                                >  1:1123833/3‑150 (MQ=255)

                                                                                                      aaTCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGACATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCg                                               >  1:357547/1‑150 (MQ=255)

                                                                                                       aTCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGc                                              <  1:1514871/150‑1 (MQ=255)

                                                                                                         cGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGTCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCtt                                            <  2:357547/150‑1 (MQ=255)

                                                                                                           tAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCAAAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTtc                                          <  1:1137928/150‑1 (MQ=255)

                                                                                                            aGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTtct                                         <  2:77229/150‑1 (MQ=255)

                                                                                                             ggCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTTTGATTTCACCGCTTtctc                                        <  2:245966/150‑1 (MQ=255)

                                                                                                                gtcgtTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGcc                                     <  2:1326655/150‑1 (MQ=255)

                                                                                                                gtcgtTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGcc                                     <  1:529571/150‑1 (MQ=255)

                                                                                                                  cgtTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAg                                   <  1:42571/150‑1 (MQ=255)

                                                                                                                  cgtTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAg                                   >  1:1123905/1‑150 (MQ=255)

                                                                                                                  cgtTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAg                                   <  1:132258/150‑1 (MQ=255)

                                                                                                                    ttAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACTCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAgtg                                 <  2:882367/150‑1 (MQ=255)

                                                                                                                     tAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAgtgt                                >  1:890393/1‑150 (MQ=255)

                                                                                                                     tAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAgtgt                                <  1:298041/150‑1 (MQ=255)

                                                                                                                     tAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAgtgt                                >  1:731806/1‑150 (MQ=255)

                                                                                                                      aaTCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGtt                               <  2:1123833/150‑1 (MQ=255)

                                                                                                                         cAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTtcat                            <  1:1239243/150‑1 (MQ=255)

                                                                                                                            cTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAg                         >  1:1075522/1‑150 (MQ=255)

                                                                                                                              tcatttcatCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCa                       <  1:704548/150‑1 (MQ=255)

                                                                                                                              tcatttcatCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCa                       <  1:1203211/150‑1 (MQ=255)

                                                                                                                               catttcatCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAt                      <  2:644474/150‑1 (MQ=255)

                                                                                                                                atttcatCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATa                     <  1:527057/150‑1 (MQ=255)

                                                                                                                                  ttcatCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATaaa                   >  2:1313133/1‑150 (MQ=255)

                                                                                                                                   ggcaggcgcATCCATTCAGTGGCTGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCACAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAag                  >  1:570266/6‑150 (MQ=255)

                                                                                                                                    catCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAagc                 >  2:560860/1‑150 (MQ=255)

                                                                                                                                     caggcgcATCCATTCAGTGGCTGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAagca                >  1:1463378/4‑150 (MQ=255)

                                                                                                                                     caggcgcATCCATTCAGTGGCTGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAagca                >  1:342441/4‑150 (MQ=255)

                                                                                                                                       cgcgCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAagcagc              >  1:1175658/1‑150 (MQ=255)

                                                                                                                                        gcgcATCCATTCAGTGGCTGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGcc             >  2:426986/1‑150 (MQ=255)

                                                                                                                                             gCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGc        <  1:560860/150‑1 (MQ=255)

                                                                                                                                               cACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCa      >  1:1248230/1‑150 (MQ=255)

                                                                                                                                                aCTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATTGTGGTCAGCATGCCGTTTTCTGATTTCACCGCTTTCTCGCCATTGTTCATCAGCATAAAGCAGCCATTGCCAt     <  2:478393/150‑1 (MQ=255)

                                                                                                                                                aCTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAG‑CAGTGCCATa    <  2:208448/150‑1 (MQ=255)

                                                                                                                                                 cTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATa    <  1:138396/150‑1 (MQ=255)

                                                                                                                                                   gAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAgg  >  1:433100/1‑150 (MQ=255)

                                                                                                                                                   gAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAgg  >  1:71244/1‑150 (MQ=255)

CCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGG  >  NC_000913/4116133‑4116429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`