Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,236,296 T→A 5.7% L12* (TTA→TAA)  yjbF → lipoprotein YjbF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,236,2960TA 5.7% 284.7 / 5.0 88L12* (TTA→TAA) yjbFlipoprotein YjbF
Reads supporting (aligned to +/- strand):  ref base T (39/44);  new base A (2/3);  total (41/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

ATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATGCAGCTTAATGGCGGGCCG  >  NC_000913/4236148‑4236441
                                                                                                                                                    |                                                                                                                                                 
ataaAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGtta                                                                                                                                                  <  1:1605292/150‑1 (MQ=255)
 taaAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGttat                                                                                                                                                 >  2:1308475/1‑150 (MQ=255)
      tatgtatCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCTGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAg                                                                                                                                            <  2:970489/150‑1 (MQ=255)
        tgtatCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGc                                                                                                                                          <  2:1308193/150‑1 (MQ=255)
         gtatCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGcc                                                                                                                                         <  2:787788/150‑1 (MQ=255)
         gtatCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGcc                                                                                                                                         <  2:1405404/150‑1 (MQ=255)
          tatCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCt                                                                                                                                        >  2:1079234/1‑150 (MQ=255)
          tatCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTAATAACAGGcag                                                                                                                                        <  2:13883/150‑3 (MQ=255)
           atCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTg                                                                                                                                       >  2:277364/1‑150 (MQ=255)
             ccccAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGtt                                                                                                                                     >  2:893050/1‑150 (MQ=255)
              cccAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTc                                                                                                                                    >  2:727694/1‑150 (MQ=255)
              cccAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTc                                                                                                                                    >  2:374629/1‑150 (MQ=255)
              cccAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTAATAACAGGcagataa                                                                                                                                    <  1:778205/150‑7 (MQ=255)
              cccAAAATAATTCGAGTCATTGCAGCTGTGGCTAGCAGTCTGAAGGGCGTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTAATAACAGGcagataa                                                                                                                                    <  1:842646/150‑7 (MQ=255)
                    ataatTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCAc                                                                                                                              >  1:1496668/1‑150 (MQ=255)
                       atTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGAc                                                                                                                           <  1:972383/150‑1 (MQ=255)
                       atTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGAc                                                                                                                           >  1:1329812/1‑150 (MQ=255)
                       atTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGAc                                                                                                                           <  1:12412/150‑1 (MQ=255)
                            aGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAag                                                                                                                      <  1:499751/150‑1 (MQ=255)
                               cATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCATATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAagagg                                                                                                                   >  1:1091615/1‑149 (MQ=255)
                                 ttGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTa                                                                                                                         <  2:53651/142‑1 (MQ=255)
                                 ttGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTa                                                                                                                         >  1:53651/1‑142 (MQ=255)
                                 ttGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTg                                                                                                                 >  1:87845/1‑150 (MQ=255)
                                  tGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGGGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTgg                                                                                                                <  2:1029267/150‑1 (MQ=255)
                                    cATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGc                                                                                                              <  1:1539480/150‑1 (MQ=255)
                                      tCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCaa                                                                                                            <  1:335621/150‑1 (MQ=255)
                                      tCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCaa                                                                                                            <  2:806302/150‑1 (MQ=255)
                                        tgtgGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAAtt                                                                                                          >  2:696676/1‑150 (MQ=255)
                                             cTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACtg                                                                                                     >  1:159510/1‑150 (MQ=255)
                                              tAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGCCCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACtgt                                                                                                    >  1:1020384/1‑150 (MQ=255)
                                              tAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACtgt                                                                                                    <  1:696676/150‑1 (MQ=255)
                                                gAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTgg                                                                                                  <  1:746284/150‑1 (MQ=255)
                                                gAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTgg                                                                                                  <  1:1308819/150‑1 (MQ=255)
                                                  aGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGGCCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGa                                                                                                >  1:347246/1‑150 (MQ=255)
                                                       gAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTAACTGTGGGACAGTc                                                                                           >  1:441490/1‑150 (MQ=255)
                                                       gAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCAGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTc                                                                                           >  2:1498434/1‑150 (MQ=255)
                                                          gggATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTggg                                                                                                 <  1:1083201/141‑1 (MQ=255)
                                                          gggATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTggg                                                                                                 >  2:1083201/1‑141 (MQ=255)
                                                            gATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTc                                                                                      >  1:164598/1‑150 (MQ=255)
                                                              ttAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCgg                                                                                    <  2:1329812/150‑1 (MQ=255)
                                                                aaccataaccaCACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCa                                                                                  >  1:1352919/1‑150 (MQ=255)
                                                                aaccataaccaCACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCa                                                                                  >  2:1367322/1‑150 (MQ=255)
                                                                aaccataaccaCACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCa                                                                                  <  1:407168/150‑1 (MQ=255)
                                                                 accataaccaCACTCCGGTGTGGTTATTATGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTTCCTGTTATTACAGGCCTGTTAAGCCAAGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCAc                                                                                 >  2:595457/1‑150 (MQ=255)
                                                                  ccataaccaCACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGCCCTGCACTCATTCTTATCAGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACg                                                                                >  1:31691/1‑150 (MQ=255)
                                                                  ccataaccaCACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACg                                                                                <  1:501233/150‑1 (MQ=255)
                                                                    ataaccaCACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGcc                                                                              <  1:804204/150‑1 (MQ=255)
                                                                        ccaCACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGc                                                                          >  2:1536418/1‑150 (MQ=255)
                                                                         cacaCTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCg                                                                         <  1:1308475/150‑1 (MQ=255)
                                                                         cacaCTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCg                                                                         <  2:1496668/150‑1 (MQ=255)
                                                                         cacaCTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATATGCCTGTTATTTCAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCg                                                                         >  1:210072/1‑150 (MQ=255)
                                                                            acTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTAc                                                                      >  1:659060/1‑150 (MQ=255)
                                                                                      ggTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGga                                                            <  2:165019/150‑1 (MQ=255)
                                                                                      ggTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGga                                                            <  1:1079234/150‑1 (MQ=255)
                                                                                       gTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGAc                                                                                               <  1:1417115/114‑1 (MQ=255)
                                                                                       gTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGAc                                                                                               >  2:1417115/1‑114 (MQ=255)
                                                                                       gTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAg                                                                                             <  1:166776/116‑1 (MQ=255)
                                                                                       gTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAg                                                                                             >  2:166776/1‑116 (MQ=255)
                                                                                            tCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGgatgatga                                                      <  1:307972/150‑1 (MQ=255)
                                                                                            tCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGgatgatga                                                      >  1:1089170/1‑150 (MQ=255)
                                                                                            tCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGgatgatga                                                      <  1:277364/150‑1 (MQ=255)
                                                                                            tCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACACCTGACGgatgatga                                                      >  1:230633/1‑150 (MQ=255)
                                                                                             cTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGgatgatgat                                                     <  1:1536418/150‑1 (MQ=255)
                                                                                             cTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGgatgatgat                                                     <  2:659060/150‑1 (MQ=255)
                                                                                              tGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGata                                                    >  2:494836/1‑150 (MQ=255)
                                                                                               gCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGatat                                                   >  2:1348326/1‑150 (MQ=255)
                                                                                                 cccTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTc                                                 <  1:487338/150‑1 (MQ=255)
                                                                                                      tGGAGATGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAAATCACTGTGTGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAat                                            <  1:574325/150‑1 (MQ=255)
                                                                                                       ggAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATATGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGATCTGGGCAATTCAATGTCGGACAGTCTGTTCGGCCCGCCAGCCGTACAGCTTACGGATGATGATATTCAAAata                                           >  2:655465/1‑150 (MQ=255)
                                                                                                         agaagaGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCaaa                                              <  2:556427/145‑1 (MQ=255)
                                                                                                         agaagaGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCaaa                                              >  1:556427/1‑145 (MQ=255)
                                                                                                          gaagaGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGc                                        >  2:1627243/1‑150 (MQ=255)
                                                                                                           aagaGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGcc                                       <  2:159510/150‑1 (MQ=255)
                                                                                                             gagTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCt                                     >  2:110016/1‑150 (MQ=255)
                                                                                                             gagGCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGGTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCt                                     <  2:379126/150‑1 (MQ=255)
                                                                                                               gTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTAc                                   <  2:140316/150‑1 (MQ=255)
                                                                                                                 cGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACgc                                 >  2:612338/1‑150 (MQ=255)
                                                                                                                  gTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACgcc                                <  1:374629/150‑1 (MQ=255)
                                                                                                                    gAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACgccag                              <  1:727694/150‑1 (MQ=255)
                                                                                                                          aCCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTa                        <  1:494836/150‑1 (MQ=255)
                                                                                                                           ccTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTAc                       <  2:347246/150‑1 (MQ=255)
                                                                                                                                    aTTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATGCAGCtt              <  2:1622467/150‑1 (MQ=255)
                                                                                                                                      tCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATGCAGCTTaa            <  2:31691/150‑1 (MQ=255)
                                                                                                                                        gaacaggCCTGTAATAACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATGCAGCTTAATg          >  2:1452629/7‑150 (MQ=255)
                                                                                                                                         aacaggCCTGTAATAACAGGCCTGTTCAGCCACGACTAATGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATGCAGCTTAATgg         >  2:185520/6‑150 (MQ=255)
                                                                                                                                          aTCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATGCAGCTTAATGGc        >  1:1192508/1‑150 (MQ=255)
                                                                                                                                               ccTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATGCAGCTTAATGGCGGGcc   <  2:193651/150‑1 (MQ=255)
                                                                                                                                                cTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACgccag                              <  1:1204440/122‑1 (MQ=255)
                                                                                                                                                cTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACgccag                              >  2:1204440/1‑122 (MQ=255)
                                                                                                                                                cTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATGCAGCTTAATGGCGGGCCg  >  1:1079152/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATTACAGGCCTGTTCAGCCACGACTAAAGAGCTGGGCAATTCACTGTGGGACAGTCTGTTCGGCACGCCAGGCGTACAGCTGACGGATGATGATATTCAAAATATGCCCTACGCCAGCCAGTACATGCAGCTTAATGGCGGGCCG  >  NC_000913/4236148‑4236441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑