Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,381,396 G→C 5.5% P308R (CCG→CGG)  frdA ← fumarate reductase flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,381,3960GC 5.5% 354.6 / 7.0 105P308R (CCG→CGG) frdAfumarate reductase flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (47/51);  new base C (2/4);  total (50/55)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 106 total aligned reads displayed.

GTGCGGTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATT  >  NC_000913/4381253‑4381544
                                                                                                                                               |                                                                                                                                                    
gtgCGGTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGTGATAAACCACATCGCCACGCGGCGTgg                                                                                                                                                <  1:182138/150‑1 (MQ=255)
gtgCGGTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTgg                                                                                                                                                >  2:1046356/1‑150 (MQ=255)
   cGGTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGCCGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGgaga                                                                                                                                             >  1:1033560/1‑150 (MQ=255)
   cGGTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGgaga                                                                                                                                             >  1:1456622/1‑150 (MQ=255)
    ggTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGAt                                                                                                                                            >  2:1565614/1‑150 (MQ=255)
      tCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATgg                                                                                                                                          <  1:1051436/150‑1 (MQ=255)
      tCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATgg                                                                                                                                          <  2:1260635/150‑1 (MQ=255)
       cGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCTCCACGCCGCGTGGCGATgtg                                                                                                                                         <  2:720366/150‑3 (MQ=255)
       cGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCTCCACGCCGCGTGGCGATgtg                                                                                                                                         <  1:1507672/150‑3 (MQ=255)
         gACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCTCCACGCCGCGTGGCGATgtggt                                                                                                                                       <  2:189904/150‑5 (MQ=255)
            gttccGGATTCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAAGGGCAGACGTTCATGCAGTTTTTTCTCGCGGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGGTGc                                                                                                                                    <  2:133792/147‑1 (MQ=255)
            gTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGc                                                                                                                                    >  1:265660/1‑150 (MQ=255)
             tACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGcc                                                                                                                                   <  1:1565614/150‑1 (MQ=255)
               ccGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCtt                                                                                                                                 >  2:512659/1‑150 (MQ=255)
               ccGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCtt                                                                                                                                 >  1:777710/1‑150 (MQ=255)
                 ggAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTa                                                                                                                               >  2:1134762/1‑150 (MQ=255)
                 ggAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTa                                                                                                                               >  2:1024683/1‑150 (MQ=255)
                   aaTCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACg                                                                                                                             <  1:260428/150‑1 (MQ=255)
                       ggTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCAt                                                                                                                         >  2:140892/1‑150 (MQ=255)
                           cTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGt                                                                                                                     >  2:131855/1‑150 (MQ=255)
                            tttAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTg                                                                                                                    <  1:1046356/150‑1 (MQ=255)
                             ttAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGc                                                                                                                   <  2:530634/150‑1 (MQ=255)
                               aaCCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCa                                                                                                                 >  2:1256972/1‑150 (MQ=255)
                                   ggATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAg                                                                                                             <  1:554302/150‑1 (MQ=255)
                                   ggATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAg                                                                                                             <  1:544366/150‑1 (MQ=255)
                                    gATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTa                                                                                                                               <  2:630181/131‑1 (MQ=255)
                                    gATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTa                                                                                                                               >  1:630181/1‑131 (MQ=255)
                                        gACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCTTg                                                                                                        <  2:256348/150‑1 (MQ=255)
                                        gACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTg                                                                                                        <  2:1177772/150‑1 (MQ=255)
                                         aCGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTga                                                                                                       >  2:819210/1‑150 (MQ=255)
                                          cGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTgag                                                                                                      >  1:1558043/1‑150 (MQ=255)
                                          cGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTgag                                                                                                      <  2:71916/150‑1 (MQ=255)
                                          cGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTgag                                                                                                      <  1:808542/150‑1 (MQ=255)
                                          cGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTgag                                                                                                      <  1:728128/150‑1 (MQ=255)
                                            ccAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTgagag                                                                                                    >  1:1363599/1‑150 (MQ=255)
                                               aCGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACt                                                                                                 >  2:772510/1‑150 (MQ=255)
                                                    cGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTc                                                                                            >  2:801296/1‑150 (MQ=255)
                                                      cTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTcgc                                                                                          >  1:1354845/1‑150 (MQ=255)
                                                      cTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTcgc                                                                                          >  1:794331/1‑150 (MQ=255)
                                                       ttccGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTcgcg                                                                                         <  2:499704/147‑1 (MQ=255)
                                                                 tCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGt                                                                               <  1:1573255/150‑1 (MQ=255)
                                                                   gCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTc                                                                             >  1:1630871/1‑150 (MQ=255)
                                                                   ctgcaTGAACGTCTGCCGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTc                                                                             >  2:882930/5‑150 (MQ=255)
                                                                     gcaTGAACGTCTGCCGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCa                                                                           >  2:727882/3‑150 (MQ=255)
                                                                     aGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCa                                                                           <  2:494673/150‑1 (MQ=255)
                                                                       aTGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCata                                                                         <  2:1456622/150‑1 (MQ=255)
                                                                       aTGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCata                                                                         <  2:777710/150‑1 (MQ=255)
                                                                        tGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCatat                                                                        >  2:1606096/1‑150 (MQ=255)
                                                                         gAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCatata                                                                       >  1:1608985/1‑150 (MQ=255)
                                                                          aaCGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCatatat                                                                      >  2:1542858/1‑150 (MQ=255)
                                                                           aCGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATAtt                                                                     <  2:1571450/150‑1 (MQ=255)
                                                                            cGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATAttt                                                                    >  1:34074/1‑150 (MQ=255)
                                                                               cAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGtt                                                                 <  2:265660/150‑1 (MQ=255)
                                                                               cAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGAACCAGTTCCATATATTTGtt                                                                 <  2:180439/150‑1 (MQ=255)
                                                                                   cGTTCATGCAGTTTTTTCTGGCCGAGGTGACCCAAGTCGAGATAAACCACATCGCCTCGCGGCGTGGAGATGGTGATGGGTTTACGCCATTCGTGCCAGAAGGCCTGACAGACGTTGTCGCGTGGAGCCAGTTCCAGATATTTGTTATTc                                                             >  2:89600/1‑150 (MQ=255)
                                                                                   cGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTc                                                             <  2:1608985/150‑1 (MQ=255)
                                                                                   cGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTc                                                             >  2:601279/1‑150 (MQ=255)
                                                                                    gTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCg                                                            <  1:1542858/150‑1 (MQ=255)
                                                                                     ttCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCgg                                                           <  1:1024683/150‑1 (MQ=255)
                                                                                         tGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCg                                                       >  1:1463143/1‑150 (MQ=255)
                                                                                          gCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGc                                                      >  2:385581/1‑150 (MQ=255)
                                                                                           cAGTTTTTTCTCGCCGAGGTGACGCTAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGcc                                                     >  1:514194/1‑150 (MQ=255)
                                                                                           cAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGcc                                                     <  2:794331/150‑1 (MQ=255)
                                                                                             attttttCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACTCGGAGTGGAGATGGTGTTGCCTTTACGCAATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCa                                                   >  1:697111/2‑150 (MQ=255)
                                                                                                 tttCTCGCCGAGGTGACGCAAGTCGAGATAAACCACTTCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCgg                                               >  2:726903/1‑150 (MQ=255)
                                                                                                 tttCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCgg                                               <  1:853354/150‑1 (MQ=255)
                                                                                                   tctcGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAg                                             <  1:1134762/150‑1 (MQ=255)
                                                                                                      cGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGttt                                          >  1:895338/1‑150 (MQ=255)
                                                                                                          gAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCgg                                      <  1:131855/150‑1 (MQ=255)
                                                                                                                 cGCAAGTCGAGATAAACCACATCGCCACGCGGCTTGTAGATGGTGTTGCCTTTACGCCATTAGTGCCAGAAGGCCTGAGAGACTTTGTCCCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTAGCACAGCGGAGTTTCCGCGccaaag                               >  2:1036292/1‑146 (MQ=255)
                                                                                                                 cGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATg                               >  1:116642/1‑150 (MQ=255)
                                                                                                                 cGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATg                               >  2:1014675/1‑150 (MQ=255)
                                                                                                                  gCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGc                              <  1:188965/150‑1 (MQ=255)
                                                                                                                     aGTCGAGATAAACCACTTCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGt                           <  1:726903/150‑1 (MQ=255)
                                                                                                                     aGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGt                           <  2:913714/150‑1 (MQ=255)
                                                                                                                     aGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGt                           <  1:140892/150‑1 (MQ=255)
                                                                                                                      gTCGAGATAAACAACATCGCCACGCGGAGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGAATTTGGCGCGTGGACCCAGTTGCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTa                          >  2:634689/1‑150 (MQ=255)
                                                                                                                       tCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTaa                         >  1:1131819/1‑150 (MQ=255)
                                                                                                                        cGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAAt                        <  2:1558043/150‑1 (MQ=255)
                                                                                                                          agaTAAACCACATCGCCACGCTGCGTGGAGATGGTGTTGCATTTACGCCATTCGTGCAAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTATTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCt                      >  2:182235/1‑150 (MQ=255)
                                                                                                                          agaTAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCt                      <  2:301058/150‑1 (MQ=255)
                                                                                                                             tAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGc                   <  2:380339/150‑1 (MQ=255)
                                                                                                                             tAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGc                   <  2:1630871/150‑1 (MQ=255)
                                                                                                                             tAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGc                   <  1:1328412/150‑1 (MQ=255)
                                                                                                                             tAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGc                   <  2:1131819/150‑1 (MQ=255)
                                                                                                                             tAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGACACTTTGTCGCGTTGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGc                   <  2:697111/150‑1 (MQ=255)
                                                                                                                              caacaccaTCTCCACGCCGCGTGGCGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTg                    >  1:428125/7‑148 (MQ=255)
                                                                                                                              caacaccaTCTCCACGCCGCGTGGCGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTg                    <  2:428125/142‑1 (MQ=255)
                                                                                                                              aaaCCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCa                  <  2:1363599/150‑1 (MQ=255)
                                                                                                                              aaaCCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCa                  >  1:107876/1‑150 (MQ=255)
                                                                                                                               aaCCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAg                 <  1:1036292/150‑1 (MQ=255)
                                                                                                                                   acaTCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATaa             >  1:1322962/1‑150 (MQ=255)
                                                                                                                                    caTCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAAc            <  2:1354845/150‑1 (MQ=255)
                                                                                                                                    caTCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAAc            <  1:385581/150‑1 (MQ=255)
                                                                                                                                    caTCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAAc            <  1:1223832/150‑1 (MQ=255)
                                                                                                                                      tCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACgg          <  1:1622783/150‑1 (MQ=255)
                                                                                                                                      tCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACgg          >  1:1180462/1‑150 (MQ=255)
                                                                                                                                        gCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTa        >  1:979312/1‑150 (MQ=255)
                                                                                                                                        gCCACGCGGCGTGCAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTATCCGGGCCCATGCCGTAATCTTGCAGATAACGGTa        >  2:1530333/1‑150 (MQ=255)
                                                                                                                                              cggcgTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCAtt  >  2:196222/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
GTGCGGTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATT  >  NC_000913/4381253‑4381544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑