Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,551,422 T→A 6.8% intergenic (‑126/‑214) gntP ← / → uxuA fructuronate transporter/D‑mannonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,551,4220TA 6.8% 307.9 / 6.5 98intergenic (‑126/‑214)gntP/uxuAfructuronate transporter/D‑mannonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (51/40);  new base A (4/3);  total (55/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAGAATGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATA  >  NC_000913/4551275‑4551570
                                                                                                                                                   |                                                                                                                                                    
agagAATGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAttt                                                                                                                                                    >  1:672564/1‑150 (MQ=255)
  agaATGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTgc                                                                                                                                                  <  1:1364801/150‑1 (MQ=255)
       gTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGcaa                                                                                                                                             >  1:871379/1‑150 (MQ=255)
           aGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAATTGCAGcaaaggg                                                                                                                                         <  1:1263228/150‑5 (MQ=255)
           aGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAATTGCAGcaaaggg                                                                                                                                         <  1:1349698/150‑5 (MQ=255)
            gCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAAcc                                                                                                                                        >  2:1073703/1‑150 (MQ=255)
                 tGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATgag                                                                                                                                   <  2:1301580/150‑1 (MQ=255)
                 tGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATgag                                                                                                                                   >  1:1246796/1‑150 (MQ=255)
                   cATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGagag                                                                                                                                 >  2:381796/1‑150 (MQ=255)
                    aTAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGt                                                                                                                                >  2:558552/1‑150 (MQ=255)
                     tAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTg                                                                                                                               <  2:1329230/150‑1 (MQ=255)
                     tAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTg                                                                                                                               >  1:212754/1‑150 (MQ=255)
                        cGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGaaa                                                                                                                            >  1:1014173/1‑150 (MQ=255)
                         gTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAAt                                                                                                                           >  1:556362/1‑150 (MQ=255)
                          ttttACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAAtt                                                                                                                          >  1:1333186/1‑150 (MQ=255)
                            ttaccttaccTGGTTGAACCGTTGTTATTTTTGTCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCt                                                                                                                        <  1:848114/150‑1 (MQ=255)
                              accttacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTtg                                                                                                                      >  1:922757/1‑150 (MQ=255)
                              accttacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTtg                                                                                                                      >  1:861075/1‑150 (MQ=255)
                              accttacctGGTTGAACCGTTGTTATTTTGGCCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAAAAACCATCGATTACCCTTTGCTGCAATTTGAAGCAACAACCAAGAGAGTGAAATTCTtg                                                                                                                      >  2:70012/1‑150 (MQ=255)
                              accttacctGGTTGAACCGGTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTtg                                                                                                                      <  2:1280491/150‑1 (MQ=255)
                                cttacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTtgtg                                                                                                                    <  2:1333186/150‑1 (MQ=255)
                                 ttacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGa                                                                                                                   >  2:1215513/1‑150 (MQ=255)
                                 ttacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGa                                                                                                                   >  2:1480068/1‑150 (MQ=255)
                                      tGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTgg                                                                                                              <  1:308323/150‑1 (MQ=255)
                                          tGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTaa                                                                                                          >  2:946290/1‑150 (MQ=255)
                                           gAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAAc                                                                                                         >  1:950926/1‑150 (MQ=255)
                                           gAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAAc                                                                                                         <  2:746069/150‑1 (MQ=255)
                                             aCCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCa                                                                                                       >  2:763044/1‑150 (MQ=255)
                                                   gttATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCa                                                                                                 <  1:52752/150‑1 (MQ=255)
                                                   gttATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCa                                                                                                 >  2:1107333/1‑150 (MQ=255)
                                                    ttATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAg                                                                                                <  1:40311/150‑1 (MQ=255)
                                                     tATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGa                                                                                               <  1:79184/150‑1 (MQ=255)
                                                     tATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATCAGAGTCAAATTCTTGTGATGTGGTTAACCAATTTCAGa                                                                                               >  2:158586/1‑150 (MQ=255)
                                                      aTTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGaa                                                                                              >  2:940614/1‑150 (MQ=255)
                                                            ggCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCggg                                                                                        <  2:734700/150‑1 (MQ=255)
                                                             gCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGt                                                                                       >  2:842411/1‑150 (MQ=255)
                                                              cGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGtt                                                                                      <  1:1480068/150‑1 (MQ=255)
                                                                    gttatgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACAtt                                                                                >  2:629619/1‑149 (MQ=255)
                                                                       atgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCt                                                                             <  1:643445/150‑1 (MQ=255)
                                                                        tgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCtt                                                                            >  2:955295/1‑150 (MQ=255)
                                                                           aaaTTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTAcc                                                                         <  2:672564/150‑1 (MQ=255)
                                                                           aaaTTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTAcc                                                                         <  2:455312/150‑1 (MQ=255)
                                                                            aaTTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCa                                                                        >  2:162074/1‑150 (MQ=255)
                                                                                ggTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAg                                                                    >  2:329267/1‑150 (MQ=255)
                                                                                ggTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAg                                                                    <  1:842411/150‑1 (MQ=255)
                                                                                 gTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAgg                                                                   >  1:1460942/1‑150 (MQ=255)
                                                                                  tCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGt                                                                  <  2:982766/150‑1 (MQ=255)
                                                                                   cAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTa                                                                 >  1:999175/1‑150 (MQ=255)
                                                                                   cAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTa                                                                 <  2:922757/150‑1 (MQ=255)
                                                                                     aCCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGGGATGTGGTTAACCAATTTCAGAATTCGGGTTGAGATGTCTTACCAAAAGGTAgc                                                               >  2:1589987/1‑149 (MQ=255)
                                                                                           gttgCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTtat                                                         <  2:87883/150‑1 (MQ=255)
                                                                                           gttgCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTtat                                                         <  2:861075/150‑1 (MQ=255)
                                                                                           gttgCGATGAATGTCACATCCTCTGATCAATAACCATCGAGTACCCTTTGCTGCAATATGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGCTTGACATGTCTTACCAAAAGGTAGAACTtat                                                         <  1:468769/150‑1 (MQ=255)
                                                                                             tgCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATAc                                                       <  2:871379/150‑1 (MQ=255)
                                                                                              gCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACg                                                      >  2:1367805/1‑150 (MQ=255)
                                                                                              gCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACg                                                      <  2:131218/150‑1 (MQ=255)
                                                                                                gATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGcc                                                    >  2:1116155/1‑150 (MQ=255)
                                                                                                       gTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCAt                                             >  1:1433767/1‑150 (MQ=255)
                                                                                                         caaaTCCTCTGATCAATAACCATCGATTACCCTTTGATGAAATTTGCAGCAACAACCAAGAGAGTGAAATTCTTGTGATGTGGTAAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCGATCACAAcc                                           >  2:155669/4‑150 (MQ=255)
                                                                                                           caTCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGa                                         <  1:732389/150‑1 (MQ=255)
                                                                                                             tCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATg                                       >  1:75635/1‑150 (MQ=255)
                                                                                                             tCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATg                                       <  2:203726/150‑1 (MQ=255)
                                                                                                             tCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATg                                       <  1:940614/150‑1 (MQ=255)
                                                                                                                tctGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCAATCTCATCCGATGCaa                                    >  2:82573/1‑150 (MQ=255)
                                                                                                                  tGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCGACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACg                                  >  2:1273837/1‑150 (MQ=255)
                                                                                                                   gATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGc                                 <  1:610953/150‑1 (MQ=255)
                                                                                                                   gATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTCATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAATGTAGAACTTATACGCCATCTCATCCGATGCAACGc                                 >  1:672324/1‑150 (MQ=255)
                                                                                                                    aTCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGcc                                >  1:1477543/1‑150 (MQ=255)
                                                                                                                      cAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAg                                                                    <  1:1145392/112‑1 (MQ=255)
                                                                                                                      cAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAg                                                                    >  2:1145392/1‑112 (MQ=255)
                                                                                                                      cAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCAc                              >  2:725091/1‑150 (MQ=255)
                                                                                                                      cAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCAc                              <  1:725091/150‑1 (MQ=255)
                                                                                                                           aCCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTg                         <  2:75635/150‑1 (MQ=255)
                                                                                                                           aCCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTg                         >  1:766496/1‑150 (MQ=255)
                                                                                                                            ccATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGc                        >  1:679579/1‑150 (MQ=255)
                                                                                                                             cATCCATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTACAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCg                       >  2:1275777/1‑150 (MQ=255)
                                                                                                                              aTCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCgg                      <  1:803826/150‑1 (MQ=255)
                                                                                                                                cGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTc                    <  1:512860/150‑1 (MQ=255)
                                                                                                                                 gATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCt                   >  2:1263565/1‑150 (MQ=255)
                                                                                                                                    tACCCTTTGCTGCAATTTGCAGCAACAACCATGAGACTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgt                <  2:848606/150‑1 (MQ=255)
                                                                                                                                     ggttgttGCTGCAAATTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgtt               >  1:1297558/6‑150 (MQ=255)
                                                                                                                                      gttgttGCTGCAAATTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttg              <  2:1390273/146‑1 (MQ=255)
                                                                                                                                      cccTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttg              >  1:1272813/1‑150 (MQ=255)
                                                                                                                                       ccTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttgt             <  1:329267/150‑1 (MQ=255)
                                                                                                                                        cTTTGCTGCTATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGGCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttgtt            <  1:645261/150‑1 (MQ=255)
                                                                                                                                        cTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttgtt            <  1:1073703/150‑1 (MQ=255)
                                                                                                                                        cTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttgtt            >  2:101054/1‑150 (MQ=255)
                                                                                                                                         gttGCTGCAAATTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTc           >  1:1041654/2‑150 (MQ=255)
                                                                                                                                         gttGCTGCAAATTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTc           >  1:1359431/2‑150 (MQ=255)
                                                                                                                                           tgctgcAAATTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCAt         >  2:1181770/1‑150 (MQ=255)
                                                                                                                                             ctgcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGc                                      >  2:1149883/1‑119 (MQ=255)
                                                                                                                                             ctgcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGc                                      <  1:1149883/119‑1 (MQ=255)
                                                                                                                                             ctgcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATcc       <  1:957486/150‑1 (MQ=255)
                                                                                                                                              tgcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCg      <  1:558552/150‑1 (MQ=255)
                                                                                                                                               gcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCgg     >  1:495746/1‑150 (MQ=255)
                                                                                                                                               gcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCgg     >  1:13483/1‑150 (MQ=255)
                                                                                                                                               gcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCgg     >  2:989864/1‑150 (MQ=255)
                                                                                                                                                  aTTTGCAGCAACAACCATGACAGTGAAATTCTTGTGATTTTCTTAACAAATATCAGAATTCAGAATAACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATa  >  2:1185615/1‑150 (MQ=255)
                                                                                                                                                  aTTTACAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATa  >  1:671693/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGAGAATGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATA  >  NC_000913/4551275‑4551570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑