Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,639,904 C→G 7.0% E135Q (GAA→CAA)  arcA ← DNA‑binding transcriptional dual regulator ArcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,639,9040CG 7.0% 230.6 / 7.1 71E135Q (GAA→CAA) arcADNA‑binding transcriptional dual regulator ArcA
Reads supporting (aligned to +/- strand):  ref base C (34/31);  new base G (3/2);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01

TTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTT  >  NC_000913/4639755‑4640050
                                                                                                                                                     |                                                                                                                                                  
ttCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGAACAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTc                                                                                                                                                    <  2:1082355/150‑1 (MQ=255)
   aGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCaag                                                                                                                                                 <  1:199518/150‑2 (MQ=255)
   aGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAAc                                                                                                                                                 <  1:339039/150‑1 (MQ=255)
    gCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACg                                                                                                                                                <  2:1025885/150‑1 (MQ=255)
     cACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGc                                                                                                                                               >  2:681207/1‑150 (MQ=255)
           aCTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCG‑GGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCGTTGAAAGCTacaagt                                                                                                                                        <  2:609595/150‑5 (MQ=255)
            cTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTacgacg                                                                                                                                        >  1:899003/1‑150 (MQ=255)
            cTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCGTTGAAAGCTacaagt                                                                                                                                        <  1:531248/150‑5 (MQ=255)
                  tttGCCTGGGTTTTGACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGttcttc                                                                                                                                  >  2:225601/1‑150 (MQ=255)
                     gCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCt                                                                                                                               <  1:493742/150‑1 (MQ=255)
                     gCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCt                                                                                                                               <  1:1349363/150‑1 (MQ=255)
                     gCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCt                                                                                                                               >  1:1603051/1‑150 (MQ=255)
                      ccTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTg                                                                                                                              >  2:398122/1‑150 (MQ=255)
                      ccTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGATGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTg                                                                                                                              >  2:1353910/1‑150 (MQ=255)
                          ggTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAg                                                                                                                          >  1:573000/1‑150 (MQ=255)
                                cacaGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCa                                                                                                                    >  1:1125531/1‑150 (MQ=255)
                                    gAAGTGACGCTTGGCGCGGAACTCGCGGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGCACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGGTACGACGTTCTTCGCTGACAGTACCCAGAtt                                                                                                                <  1:1521598/150‑1 (MQ=255)
                                    gAAGTGAAGCATGGCGCGGAACTCGCTGGGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGAtt                                                                                                                <  2:612461/150‑1 (MQ=255)
                                       gTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCAt                                                                                                             >  2:376688/1‑150 (MQ=255)
                                         gAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATgg                                                                                                           <  1:1607170/150‑1 (MQ=255)
                                         gAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATgg                                                                                                           <  1:392503/150‑1 (MQ=255)
                                             cATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACg                                                                                                       <  1:170182/150‑1 (MQ=255)
                                               tGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACggg                                                                                                     <  1:1175259/150‑1 (MQ=255)
                                                   gcgGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAg                                                                                                 >  2:1607890/1‑150 (MQ=255)
                                                   gcgGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAg                                                                                                 <  1:1165678/150‑1 (MQ=255)
                                                          tCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTg                                                                                          <  1:1353910/150‑1 (MQ=255)
                                                            gCTGCGCGGCAGGTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCg                                                                                        <  1:379886/150‑1 (MQ=255)
                                                              tGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTg                                                                                      <  2:719741/150‑1 (MQ=255)
                                                               gcgcGTCATCGTGTTCTTCGCGCAATCAGGGCCTATCAAAGAACGGGTGTTGATTTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTTCGACGTTCTTGGCTGTCAGTACCGAGTTTCATGGTACGGGACTTTAGGgtgcgtgc                                                                                     <  2:697647/150‑1 (MQ=255)
                                                                   ggCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGa                                                                                 >  2:113837/1‑150 (MQ=255)
                                                                         ttGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTc                                                                           <  1:1607890/150‑1 (MQ=255)
                                                                           ttACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTAGGACGTTCTTCGCTGACAGTACCCAGATTGATTGTACGTGACAGTAGGTTGCGTGCACGAATCGTCAg                                                                         <  2:609473/149‑1 (MQ=255)
                                                                              cTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTc                                                                      >  1:1591161/1‑150 (MQ=255)
                                                                              cTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTc                                                                      <  2:1125531/150‑1 (MQ=255)
                                                                              cTGCTCGCCATCAGGGCCGATCAACGAACGGCGGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTTTTTGCTGACAGTACCCAGTTTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTc                                                                      <  2:820741/150‑1 (MQ=255)
                                                                                 cTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCAcg                                                                   >  2:616264/1‑150 (MQ=255)
                                                                                     ccATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACTGTACCCAGATTCTTGGTACGGGTCATTAGTTTGCGTGCAAGAATCTTCAGTTCAcggtgg                                                               <  2:1603051/150‑5 (MQ=255)
                                                                                           gggCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAAc                                                         <  2:157401/150‑1 (MQ=255)
                                                                                                gATCAACGAACGGCTGTTGATGTCCAGTTACCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGATGACAGTACCCAGATTCATGTTACGGTACAGTAGGTTGCGTGCACGAATCGTCAGTTAAAGCGCGTTGAACGGttt                                                    >  1:211005/1‑150 (MQ=255)
                                                                                                   cAACGAACGGCTGGTGAGGTCCAGGTCCCAGCCATTGAACTTGTAGATTAAAACGCATCGCCGTTGTTCGCAGACAGTACCCAGATTCATGGTACGGGACAGTAGGTAGCGTGCACGACTGGTAAGTTCACGAGGGTTGCACGGTTTGGt                                                 >  2:1462701/1‑150 (MQ=255)
                                                                                                    aacgaacgGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTg                                                <  1:681207/150‑1 (MQ=255)
                                                                                                     acgaacgGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGa                                               >  2:1413595/1‑150 (MQ=255)
                                                                                                            gCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTAACCAGATTCATGGTACGGGAAAGTAAGTTGAGTGCACGAATCGTCATTTAACGCCTGTTGAACGGTTTGGTGATGTAGta                                        >  2:1536647/1‑149 (MQ=255)
                                                                                                                 tGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTg                                   <  2:1108907/150‑1 (MQ=255)
                                                                                                                  gATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGc                                  >  2:559539/1‑150 (MQ=255)
                                                                                                                   aTGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCa                                 >  2:1256920/1‑150 (MQ=255)
                                                                                                                       ccAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGa                             >  1:532579/1‑150 (MQ=255)
                                                                                                                       ccAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGa                             >  2:419886/1‑150 (MQ=255)
                                                                                                                            tCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCg                        >  2:857476/1‑150 (MQ=255)
                                                                                                                             cccAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGa                       >  1:1177100/1‑150 (MQ=255)
                                                                                                                             cccAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGa                       <  2:754896/150‑1 (MQ=255)
                                                                                                                             cccAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGa                       <  2:1008371/150‑1 (MQ=255)
                                                                                                                             cccAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACACAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGa                       >  1:593943/1‑150 (MQ=255)
                                                                                                                             cccAACAATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGa                       >  2:1320265/1‑150 (MQ=255)
                                                                                                                              ccAACCATTGAACTTGTAGGTTTCTACGCTACGACGTTCTTCGCTGACAGTACCGAGATTCATTGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAg                      <  1:1594168/150‑1 (MQ=255)
                                                                                                                              ccAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAg                      >  1:1466464/1‑150 (MQ=255)
                                                                                                                               cAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAgg                     >  1:1347627/1‑150 (MQ=255)
                                                                                                                               cAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAgg                     >  1:514644/1‑150 (MQ=255)
                                                                                                                                aaCCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGTATGAATCTTCTTTTCACGCGGGTTGAACTGTTTGGTGATGTAGTCATCTTCACCTAtttgtaggt                    <  2:268346/150‑7 (MQ=255)
                                                                                                                                    aTTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGAAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCgag                <  1:1273179/150‑1 (MQ=255)
                                                                                                                                     ttGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTAACCAGATTAATGGTACGGGACAGTAGGTTGCGTGCAAGAATCGTCAGTTCACGCGGGATCAACGGTTTGGAGATGTAGTCATCTGCACCGATTTCGACGCCgaga               >  2:326191/1‑150 (MQ=255)
                                                                                                                                       gAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAAt             >  1:1421107/1‑150 (MQ=255)
                                                                                                                                        acgtcgTAGCGTTGAAAGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAAtt            >  1:1182152/6‑150 (MQ=255)
                                                                                                                                        acgtcgTAGCGTTGAAAGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAAtt            >  2:21820/6‑150 (MQ=255)
                                                                                                                                        acgtcgTAGCGTTGAAAGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATAGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAAtt            >  1:1607692/6‑150 (MQ=255)
                                                                                                                                         aCTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAAttt           <  1:749352/150‑1 (MQ=255)
                                                                                                                                             gTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATc       >  2:850838/1‑150 (MQ=255)
                                                                                                                                              tAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCg      <  1:113837/150‑1 (MQ=255)
                                                                                                                                               aGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGa     >  2:1246518/1‑150 (MQ=255)
                                                                                                                                               aGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGa     >  2:1366178/1‑150 (MQ=255)
                                                                                                                                                  tttCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGACCGGATTGGTGATGGAGTCAGCTGCACCGATTTCGAGGCCGAGAATTTTATCGACtt  >  2:1588450/1‑150 (MQ=255)
                                                                                                                                                  tttCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACtt  >  1:765633/1‑150 (MQ=255)
                                                                                                                                                     cAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCa                                       <  1:1327758/110‑1 (MQ=255)
                                                                                                                                                     cAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCa                                       >  2:1327758/1‑110 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTT  >  NC_000913/4639755‑4640050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑