Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,639,907 C→A 7.0% V134F (GTT→TTT)  arcA ← DNA‑binding transcriptional dual regulator ArcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,639,9070CA 7.0% 236.9 / 5.7 71V134F (GTT→TTT) arcADNA‑binding transcriptional dual regulator ArcA
Reads supporting (aligned to +/- strand):  ref base C (36/30);  new base A (3/2);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

AGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGT  >  NC_000913/4639758‑4640056
                                                                                                                                                     |                                                                                                                                                     
aGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAAc                                                                                                                                                       <  1:339039/150‑1 (MQ=255)
 gCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACg                                                                                                                                                      <  2:1025885/150‑1 (MQ=255)
  cACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGc                                                                                                                                                     >  2:681207/1‑150 (MQ=255)
        aCTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCG‑GGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCGTTGAAAGCTacaagt                                                                                                                                              <  2:609595/150‑5 (MQ=255)
         cTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTacgacg                                                                                                                                              >  1:899003/1‑150 (MQ=255)
         cTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCGTTGAAAGCTacaagt                                                                                                                                              <  1:531248/150‑5 (MQ=255)
               tttGCCTGGGTTTTGACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGttcttc                                                                                                                                        >  2:225601/1‑150 (MQ=255)
                  gCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCt                                                                                                                                     <  1:493742/150‑1 (MQ=255)
                  gCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCt                                                                                                                                     <  1:1349363/150‑1 (MQ=255)
                  gCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCt                                                                                                                                     >  1:1603051/1‑150 (MQ=255)
                   ccTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTg                                                                                                                                    >  2:398122/1‑150 (MQ=255)
                   ccTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGATGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTg                                                                                                                                    >  2:1353910/1‑150 (MQ=255)
                       ggTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAg                                                                                                                                >  1:573000/1‑150 (MQ=255)
                             cacaGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCa                                                                                                                          >  1:1125531/1‑150 (MQ=255)
                                 gAAGTGACGCTTGGCGCGGAACTCGCGGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGCACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGGTACGACGTTCTTCGCTGACAGTACCCAGAtt                                                                                                                      <  1:1521598/150‑1 (MQ=255)
                                 gAAGTGAAGCATGGCGCGGAACTCGCTGGGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGAtt                                                                                                                      <  2:612461/150‑1 (MQ=255)
                                    gTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCAt                                                                                                                   >  2:376688/1‑150 (MQ=255)
                                      gAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATgg                                                                                                                 <  1:1607170/150‑1 (MQ=255)
                                      gAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATgg                                                                                                                 <  1:392503/150‑1 (MQ=255)
                                          cATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACg                                                                                                             <  1:170182/150‑1 (MQ=255)
                                            tGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACggg                                                                                                           <  1:1175259/150‑1 (MQ=255)
                                                gcgGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAg                                                                                                       <  1:1165678/150‑1 (MQ=255)
                                                gcgGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAg                                                                                                       >  2:1607890/1‑150 (MQ=255)
                                                       tCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTg                                                                                                <  1:1353910/150‑1 (MQ=255)
                                                         gCTGCGCGGCAGGTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCg                                                                                              <  1:379886/150‑1 (MQ=255)
                                                           tGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTg                                                                                            <  2:719741/150‑1 (MQ=255)
                                                            gcgcGTCATCGTGTTCTTCGCGCAATCAGGGCCTATCAAAGAACGGGTGTTGATTTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTTCGACGTTCTTGGCTGTCAGTACCGAGTTTCATGGTACGGGACTTTAGGgtgcgtgc                                                                                           <  2:697647/150‑1 (MQ=255)
                                                                ggCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGa                                                                                       >  2:113837/1‑150 (MQ=255)
                                                                      ttGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTc                                                                                 <  1:1607890/150‑1 (MQ=255)
                                                                        ttACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTAGGACGTTCTTCGCTGACAGTACCCAGATTGATTGTACGTGACAGTAGGTTGCGTGCACGAATCGTCAg                                                                               <  2:609473/149‑1 (MQ=255)
                                                                           cTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTc                                                                            <  2:1125531/150‑1 (MQ=255)
                                                                           cTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTc                                                                            >  1:1591161/1‑150 (MQ=255)
                                                                           cTGCTCGCCATCAGGGCCGATCAACGAACGGCGGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTTTTTGCTGACAGTACCCAGTTTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTc                                                                            <  2:820741/150‑1 (MQ=255)
                                                                              cTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCAcg                                                                         >  2:616264/1‑150 (MQ=255)
                                                                                  ccATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACTGTACCCAGATTCTTGGTACGGGTCATTAGTTTGCGTGCAAGAATCTTCAGTTCAcggtgg                                                                     <  2:1603051/150‑5 (MQ=255)
                                                                                        gggCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAAc                                                               <  2:157401/150‑1 (MQ=255)
                                                                                             gATCAACGAACGGCTGTTGATGTCCAGTTACCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGATGACAGTACCCAGATTCATGTTACGGTACAGTAGGTTGCGTGCACGAATCGTCAGTTAAAGCGCGTTGAACGGttt                                                          >  1:211005/1‑150 (MQ=255)
                                                                                                cAACGAACGGCTGGTGAGGTCCAGGTCCCAGCCATTGAACTTGTAGATTAAAACGCATCGCCGTTGTTCGCAGACAGTACCCAGATTCATGGTACGGGACAGTAGGTAGCGTGCACGACTGGTAAGTTCACGAGGGTTGCACGGTTTGGt                                                       >  2:1462701/1‑150 (MQ=255)
                                                                                                 aacgaacgGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTg                                                      <  1:681207/150‑1 (MQ=255)
                                                                                                  acgaacgGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGa                                                     >  2:1413595/1‑150 (MQ=255)
                                                                                                         gCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTAACCAGATTCATGGTACGGGAAAGTAAGTTGAGTGCACGAATCGTCATTTAACGCCTGTTGAACGGTTTGGTGATGTAGta                                              >  2:1536647/1‑149 (MQ=255)
                                                                                                              tGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTg                                         <  2:1108907/150‑1 (MQ=255)
                                                                                                               gATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGc                                        >  2:559539/1‑150 (MQ=255)
                                                                                                                aTGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCa                                       >  2:1256920/1‑150 (MQ=255)
                                                                                                                    ccAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGa                                   >  2:419886/1‑150 (MQ=255)
                                                                                                                    ccAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGa                                   >  1:532579/1‑150 (MQ=255)
                                                                                                                         tCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCg                              >  2:857476/1‑150 (MQ=255)
                                                                                                                          cccAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGa                             <  2:754896/150‑1 (MQ=255)
                                                                                                                          cccAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGa                             <  2:1008371/150‑1 (MQ=255)
                                                                                                                          cccAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGa                             >  1:1177100/1‑150 (MQ=255)
                                                                                                                          cccAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACACAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGa                             >  1:593943/1‑150 (MQ=255)
                                                                                                                          cccAACAATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGa                             >  2:1320265/1‑150 (MQ=255)
                                                                                                                           ccAACCATTGAACTTGTAGGTTTCTACGCTACGACGTTCTTCGCTGACAGTACCGAGATTCATTGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAg                            <  1:1594168/150‑1 (MQ=255)
                                                                                                                           ccAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAg                            >  1:1466464/1‑150 (MQ=255)
                                                                                                                            cAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAgg                           >  1:514644/1‑150 (MQ=255)
                                                                                                                            cAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAgg                           >  1:1347627/1‑150 (MQ=255)
                                                                                                                             aaCCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGTATGAATCTTCTTTTCACGCGGGTTGAACTGTTTGGTGATGTAGTCATCTTCACCTAtttgtaggt                          <  2:268346/150‑7 (MQ=255)
                                                                                                                                 aTTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGAAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCgag                      <  1:1273179/150‑1 (MQ=255)
                                                                                                                                  ttGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTAACCAGATTAATGGTACGGGACAGTAGGTTGCGTGCAAGAATCGTCAGTTCACGCGGGATCAACGGTTTGGAGATGTAGTCATCTGCACCGATTTCGACGCCgaga                     >  2:326191/1‑150 (MQ=255)
                                                                                                                                    gAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAAt                   >  1:1421107/1‑150 (MQ=255)
                                                                                                                                     acgtcgTAGCGTTGAAAGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAAtt                  >  2:21820/6‑150 (MQ=255)
                                                                                                                                     acgtcgTAGCGTTGAAAGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAAtt                  >  1:1182152/6‑150 (MQ=255)
                                                                                                                                     acgtcgTAGCGTTGAAAGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATAGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAAtt                  >  1:1607692/6‑150 (MQ=255)
                                                                                                                                      aCTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAAttt                 <  1:749352/150‑1 (MQ=255)
                                                                                                                                          gTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATc             >  2:850838/1‑150 (MQ=255)
                                                                                                                                           tAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCg            <  1:113837/150‑1 (MQ=255)
                                                                                                                                            aGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGa           >  2:1246518/1‑150 (MQ=255)
                                                                                                                                            aGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGa           >  2:1366178/1‑150 (MQ=255)
                                                                                                                                               tttCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGACCGGATTGGTGATGGAGTCAGCTGCACCGATTTCGAGGCCGAGAATTTTATCGACtt        >  2:1588450/1‑150 (MQ=255)
                                                                                                                                               tttCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACtt        >  1:765633/1‑150 (MQ=255)
                                                                                                                                                  cAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCa                                             <  1:1327758/110‑1 (MQ=255)
                                                                                                                                                  cAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCa                                             >  2:1327758/1‑110 (MQ=255)
                                                                                                                                                     cGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGt  >  1:683638/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGT  >  NC_000913/4639758‑4640056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑