Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 621,499 C→G 8.0% G231R (GGC→CGC)  fepD ← ferric enterobactin ABC transporter membrane subunit FebD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913621,4990CG 8.0% 221.8 / 7.6 71G231R (GGC→CGC) fepDferric enterobactin ABC transporter membrane subunit FebD
Reads supporting (aligned to +/- strand):  ref base C (30/34);  new base G (3/3);  total (33/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGCGATGATCGGCACCCACCAGCCAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCA  >  NC_000913/621350‑621643
                                                                                                                                                     |                                                                                                                                                
ccAGCGATGATCGGCACCCACCAGCCAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGcc                                                                                                                                                  <  1:236319/150‑1 (MQ=255)
  agagatgatCGGCCCCAACCAGACAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGAACTACCACAAAGCAAGGTAATCGCCAGCAGACCAATCAACTGAGTGCGCGCCACGCGACTGACCa                                                                                                                                                >  2:1421012/4‑150 (MQ=255)
   gCGATGATCGGCACCCACCAGCCAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAg                                                                                                                                               >  2:836921/1‑150 (MQ=255)
         atCGGCACCCACCAGCCAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGt                                                                                                                                         >  2:1268080/1‑150 (MQ=255)
              cacccaccAGCCAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCgg                                                                                                                                    <  1:1605561/150‑1 (MQ=255)
              cacccaccAGCCAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCgg                                                                                                                                    >  2:1149511/1‑150 (MQ=255)
               acccaccAGCCAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACGGCGCTGGGCAGTcgcgtg                                                                                                                                   <  1:997626/150‑3 (MQ=255)
                cccaccAGCCAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGgtg                                                                                                                                  <  1:293987/150‑1 (MQ=255)
                  caccaGCCAACGCGCCATTTGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCTTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCACAGCGCCGTCGCGGTGTc                                                                                                                                <  2:1345324/150‑1 (MQ=255)
                  caccaGCCAACGCGCCATATGGGGCATGATCATGCCAATAAAGGCAATCTGGCCAACTATTGCCGTGGGACTACCACAAAGGACGGTAATCGCCAGCAGACGAATCATCTGTGTGCTCGCCAGGCGACTGCCCAGCGCCGTCGCGGTGTc                                                                                                                                <  1:1552353/150‑1 (MQ=255)
                  caccaGCCAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACTGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTc                                                                                                                                >  2:875096/1‑150 (MQ=255)
                  caccaGCCAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGCCCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTc                                                                                                                                >  1:1332229/1‑150 (MQ=255)
                  caccaGCCAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTc                                                                                                                                >  1:1535184/1‑150 (MQ=255)
                   accaGCCAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCg                                                                                                                               <  1:1252266/150‑1 (MQ=255)
                    ccaGCCAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACGGCGCTGGGCAGTcgcgtggcgcg                                                                                                                              <  2:1228865/150‑8 (MQ=255)
                     caGCCAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCt                                                                                                                             >  1:1395820/1‑150 (MQ=255)
                      aGCCAAGGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTg                                                                                                                            <  2:1330663/150‑1 (MQ=255)
                         cAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCg                                                                                                                         >  2:481160/1‑150 (MQ=255)
                         cAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCACTGCCg                                                                                                                         >  1:361555/1‑150 (MQ=255)
                             gcgcCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGc                                                                                                                     <  2:492650/150‑1 (MQ=255)
                                   tatGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAAc                                                                                                               >  2:1237175/1‑150 (MQ=255)
                                        ggCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTc                                                                                                          <  2:1082575/150‑1 (MQ=255)
                                          catcatCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAg                                                                                                        <  1:875096/150‑1 (MQ=255)
                                             catcaGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAgcgc                                                                                                     <  1:1268080/150‑1 (MQ=255)
                                             catcaGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAgcgc                                                                                                     <  1:41259/150‑1 (MQ=255)
                                             catcaGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAgcgc                                                                                                     >  1:737708/1‑150 (MQ=255)
                                              atcaGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAgcgcg                                                                                                    <  2:1494403/150‑1 (MQ=255)
                                               tcaGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAgcgcgc                                                                                                   >  2:1477937/1‑150 (MQ=255)
                                                    ccAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTc                                                                                              <  2:1108265/150‑1 (MQ=255)
                                                     cAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCa                                                                                             >  1:1097387/1‑150 (MQ=255)
                                                      aaTAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAg                                                                                            >  2:77900/1‑150 (MQ=255)
                                                        tAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTa                                                                                          <  2:1535184/150‑1 (MQ=255)
                                                         aaaGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTaa                                                                                         >  2:1249828/1‑150 (MQ=255)
                                                          aaGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAAt                                                                                        <  1:845430/150‑1 (MQ=255)
                                                            ggCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAg                                                                                      <  1:1214557/150‑1 (MQ=255)
                                                            ggCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAg                                                                                      <  1:695287/150‑1 (MQ=255)
                                                               aaTCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAgcgc                                                                                   <  2:470237/150‑1 (MQ=255)
                                                                              tGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGAt                                                                    <  1:836921/150‑1 (MQ=255)
                                                                               gCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATc                                                                   >  1:631867/1‑150 (MQ=255)
                                                                               gCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATc                                                                   <  2:631867/150‑1 (MQ=255)
                                                                                ccGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCa                                                                  >  1:1468661/1‑150 (MQ=255)
                                                                                  gTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTcgc                                                                                                                                      <  1:1289363/80‑1 (MQ=255)
                                                                                  gTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTcgc                                                                                                                                      >  2:1289363/1‑80 (MQ=255)
                                                                                  gTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGc                                                                <  1:1446089/150‑1 (MQ=255)
                                                                                         tACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGa                                                         <  2:1214085/150‑1 (MQ=255)
                                                                                           ccACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATc                                                       <  2:1274830/150‑1 (MQ=255)
                                                                                           ccACAAAGCACGGTAATCGCCAACAGACCAATCAACTGTGTGCGCGCCACCCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGCCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCCGCGATCAGCACCGGGATc                                                       >  2:1490520/1‑150 (MQ=255)
                                                                                             acaAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAg                                                     <  1:593265/150‑1 (MQ=255)
                                                                                              caAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGc                                                    <  1:1506939/150‑1 (MQ=255)
                                                                                                 aGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGcacc                                                 >  2:1242257/1‑150 (MQ=255)
                                                                                                        tAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTa                                          >  2:50031/1‑150 (MQ=255)
                                                                                                        tAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTa                                          <  1:481160/150‑1 (MQ=255)
                                                                                                         aaTCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTaa                                         >  1:1022591/1‑150 (MQ=255)
                                                                                                         aaTCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTaa                                         >  1:893782/1‑150 (MQ=255)
                                                                                                         aaTCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCACCGCCGTCGCGGTGTCGCTGCCCAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCAACACttaaaa                                         >  1:908078/1‑146 (MQ=255)
                                                                                                          aTCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAg                                        <  1:77900/150‑1 (MQ=255)
                                                                                                                      ccAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTg                            >  1:1380642/1‑150 (MQ=255)
                                                                                                                          tCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGaa                        <  1:1149511/150‑1 (MQ=255)
                                                                                                                           cAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGGCGCGGTGGCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAat                       <  2:391871/150‑1 (MQ=255)
                                                                                                                             aCTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAatat                     >  2:196130/1‑150 (MQ=255)
                                                                                                                                  gtgCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCTCGCGACTCAGTAATAGAGGAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGc                <  1:1490520/150‑1 (MQ=255)
                                                                                                                                  gtgCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGc                <  2:1332229/150‑1 (MQ=255)
                                                                                                                                   tgCGCGCCTCGCGACTGCGCAGCGCCGTCGCGGTGTCGCTGCCGAGGCGCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCg               <  2:267773/150‑1 (MQ=255)
                                                                                                                                   tgCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCTCAGTTGCTCCGGCGATCAGCACCGTGATCAGCACCACTTTTAAGGTATGTAGATTGCTAATATCCAGCg               <  2:1097387/150‑1 (MQ=255)
                                                                                                                                     cgcgcCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGaa             >  1:445218/1‑150 (MQ=255)
                                                                                                                                       cgcCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAAcc           >  1:565360/1‑150 (MQ=255)
                                                                                                                                         caccgcgACGGCGCTGGGCAGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCgg         >  1:1324301/4‑150 (MQ=255)
                                                                                                                                          cACGCGACTGCCCAGCGCCGTCGCGGTGTCACTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGc        <  2:361555/150‑1 (MQ=255)
                                                                                                                                           ccgcgACGGCGCTGGGCAGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGACCCGGCt       >  2:858788/2‑150 (MQ=255)
                                                                                                                                           ccgcgACGGCGCTGGGCAGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCt       >  1:1169237/2‑150 (MQ=255)
                                                                                                                                               gACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGcc   <  1:1452037/150‑1 (MQ=255)
                                                                                                                                                aCTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCCCCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCa  >  1:1134686/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
CCAGCGATGATCGGCACCCACCAGCCAACGCGCCATATGCGGCATCATCAGGCCAATAAAGGCAATCGGGCCAACTATTGCCGTCGCACTACCACAAAGCACGGTAATCGCCAGCAGACCAATCAACTGTGTGCGCGCCACGCGACTGCCCAGCGCCGTCGCGGTGTCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCA  >  NC_000913/621350‑621643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑