Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	768,921	C→T	5.3%	T315M (ACG→ATG)	mngB →	alpha‑mannosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	768,921	0	C	T	5.3%	237.7 / 2.1	75	T315M (ACG→ATG)	mngB	alpha‑mannosidase
Reads supporting (aligned to +/- strand):  ref base C (37/34);  new base T (2/2);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

GCAACCCTGAAAGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATG  >  NC_000913/768773‑769066                                                                                                                                                     |                                                                                                                                                  gCAACCCTGAAAGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACg                                                                                                                                                  >  1:313196/1‑150 (MQ=255)     cccTGAAAGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTgg                                                                                                                                              >  2:39767/1‑150 (MQ=255)       cTGAAAGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGt                                                                                                                                            >  1:887360/1‑150 (MQ=255)         gAAAGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTACCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGttt                                                                                                                                          >  2:689720/1‑150 (MQ=255)          aaaGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGtttt                                                                                                                                         <  1:1248945/150‑1 (MQ=255)           aaGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTg                                                                                                                                        <  2:724811/150‑1 (MQ=255)           aaGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTg                                                                                                                                        <  1:538218/150‑1 (MQ=255)            aGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGTGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGa                                                                                                                                       <  1:1155084/150‑1 (MQ=255)             ggggAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGATGTTGGGTTTTGaa                                                                                                                                      >  2:991136/1‑150 (MQ=255)             ggggAATTTATTGATGGCAAATATATGCGCGTGCATAGCACAATCGGTTCTACGCGTATGGATATCAAAATTGCACACGCGCGTTTTGAAAATAAGATTGTTAATATGCTGGATCCGCTGGAAAAACTGGCATGGATGTTGGGTTTTGaa                                                                                                                                      >  2:985052/1‑150 (MQ=255)              gggAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAAt                                                                                                                                     <  2:1215608/150‑1 (MQ=255)               ggAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTg                                                                                                                                        >  1:806113/1‑146 (MQ=255)               ggAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTg                                                                                                                                        <  2:806113/146‑1 (MQ=255)                 aaTTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATacc                                                                                                                                  <  2:1289037/150‑1 (MQ=255)                  aTTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATacca                                                                                                                                 <  1:938906/150‑1 (MQ=255)                                  tatatGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGgag                                                                                                                 >  2:1537654/1‑150 (MQ=255)                                   atatGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGgaga                                                                                                                <  1:832331/150‑1 (MQ=255)                                    tatGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGACCCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGaa                                                                                                               >  2:527613/1‑150 (MQ=255)                                    tatGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGaa                                                                                                               <  1:384627/150‑1 (MQ=255)                                    catGCGCCTGCATCCCACCATCGGTTCTACGCGTATGGACATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGaa                                                                                                               <  2:682665/149‑1 (MQ=255)                                     atGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGaaa                                                                                                              <  1:1202820/150‑1 (MQ=255)                                          cgTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAAtgtg                                                                                                         <  1:218436/150‑1 (MQ=255)                                            tGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGttt                                                                                                                                          <  1:1366469/115‑1 (MQ=255)                                            tGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGttt                                                                                                                                          >  2:1366469/1‑115 (MQ=255)                                            tGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACGACGGCTTGCTGGAGAAAATGTGGa                                                                                                       >  1:162373/1‑150 (MQ=255)                                            tGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGa                                                                                                       <  1:441424/150‑1 (MQ=255)                                                tCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAaga                                                                                                   >  1:607774/1‑150 (MQ=255)                                                  gCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAgaga                                                                                                 >  2:1274749/1‑150 (MQ=255)                                                   ccccAGCGGTTCTACGCGTATGGATATCAAAATTGCCCCCGCGCGTATTGAAAATAAGATTGGTAATCTGCGGGCACCGCTGGCAACCCTGGCCTGGACGTGGGGTTGTGACTACCCCCACGGCGTGCGGGAGAAAATGTGGAAAgagag                                                                                                >  2:429310/3‑149 (MQ=255)                                                       aTCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTa                                                                                            >  1:339514/1‑150 (MQ=255)                                                                 cgcgTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGATGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATg                                                                                  >  2:224271/1‑150 (MQ=255)                                                                 cgcgTATGGATATCAAAATTACCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATg                                                                                  <  1:689720/150‑1 (MQ=255)                                                                  gcgTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGc                                                                                 <  2:1083268/150‑1 (MQ=255)                                                                     tATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCa                                                                              <  1:397913/150‑1 (MQ=255)                                                                        ggATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGa                                                                           >  2:297071/1‑150 (MQ=255)                                                                         gATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGAc                                                                          <  1:957783/150‑1 (MQ=255)                                                                         gATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGAc                                                                          >  2:644192/1‑150 (MQ=255)                                                                           tatCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAg                                                                        <  2:1576809/150‑1 (MQ=255)                                                                            atCAAAATTGCCCACGCGCGTATTGAAAAAAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGt                                                                       >  2:302212/1‑150 (MQ=255)                                                                              cAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTAt                                                                     >  1:548733/1‑150 (MQ=255)                                                                              cAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTAt                                                                     >  2:254859/1‑150 (MQ=255)                                                                                   ttGCCCCCGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGACCCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGgct                                                                <  2:1099439/150‑1 (MQ=255)                                                                                     gCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGgctgc                                                              <  2:607774/150‑1 (MQ=255)                                                                                     gCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGgctgc                                                              <  1:752886/150‑1 (MQ=255)                                                                                        cACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGgctgctgc                                                           <  1:1541419/150‑1 (MQ=255)                                                                                        cACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGgctgctgc                                                           <  2:887360/150‑1 (MQ=255)                                                                                          cgcgcgTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGctgctgctg                                                         >  1:933725/1‑150 (MQ=255)                                                                                              cgTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGTAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGt                                                     <  1:396235/150‑1 (MQ=255)                                                                                               gTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACGACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTg                                                    <  2:162373/150‑1 (MQ=255)                                                                                               gTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTg                                                    <  2:313196/150‑1 (MQ=255)                                                                                               gTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTg                                                    <  2:457003/150‑1 (MQ=255)                                                                                                tATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGa                                                   >  1:1190525/1‑150 (MQ=255)                                                                                                 aTTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGAc                                                  >  2:184855/1‑150 (MQ=255)                                                                                                    taaaaTAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGATGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACaaa                                               <  1:991136/149‑1 (MQ=255)                                                                                                    gAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCACCACTGGCCTGGACGTTGGGGTTTGAATACCACCACGGCTGGCGGGAGAAAATGTGGAAAGAGATCGTAAAAAATCATGCCCACGCCAGTAGCGGCTGCTGCGGTAGTGCCaga                                               >  2:1474964/1‑148 (MQ=255)                                                                                                    gAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGATGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACaaa                                               <  1:985052/150‑1 (MQ=255)                                                                                                     aaaaTAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAg                                              >  2:1361410/1‑150 (MQ=255)                                                                                                     aaaaTAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAg                                              >  1:810391/1‑150 (MQ=255)                                                                                                      aaaTAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGt                                             >  1:4047/1‑150 (MQ=255)                                                                                                      aaaTAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGt                                             >  2:562875/1‑150 (MQ=255)                                                                                                                gTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGaa                                   <  1:1537654/150‑1 (MQ=255)                                                                                                                   aaTCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAAtc                                >  1:1504560/1‑150 (MQ=255)                                                                                                                   aaTCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAAtc                                <  2:347061/150‑1 (MQ=255)                                                                                                                    aTCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGGTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAAtcg                               >  2:307405/1‑150 (MQ=255)                                                                                                                    aTCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAAtcg                               >  1:1057022/1‑150 (MQ=255)                                                                                                                         ctgGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGcc                          >  2:310794/1‑150 (MQ=255)                                                                                                                               ccGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTc                    >  1:1163738/1‑150 (MQ=255)                                                                                                                               ccGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTc                    >  1:10995/1‑150 (MQ=255)                                                                                                                                   tGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAAc                <  2:1163738/150‑1 (MQ=255)                                                                                                                                     gCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTg              >  2:1167147/1‑150 (MQ=255)                                                                                                                                             ggCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGc            <  1:1006656/144‑1 (MQ=255)                                                                                                                                             ggCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGc            >  2:1006656/1‑144 (MQ=255)                                                                                                                                              gCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGAc     >  1:113685/1‑150 (MQ=255)                                                                                                                                                cTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACAt   <  1:297071/150‑1 (MQ=255)                                                                                                                                                 tGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATg  >  1:1358737/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GCAACCCTGAAAGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATG  >  NC_000913/768773‑769066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑