Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,002,700	G→T	5.8%	V298V (GTG→GTT)	elfG →	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,002,700	0	G	T	5.8%	227.1 / 3.3	69	V298V (GTG→GTT)	elfG	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base G (34/31);  new base T (2/2);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-01

GCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGATTTGAAGC  >  NC_000913/1002557‑1002846                                                                                                                                                |                                                                                                                                                   gCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGAcc                                                                                                                                              <  1:157513/150‑1 (MQ=255)   cAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGTAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCGAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGa                                                                                                                                            <  2:1421314/150‑1 (MQ=255)             gAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGaac                                                                                                                                  <  2:480039/150‑1 (MQ=255)                cTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGaacaac                                                                                                                               >  1:1150308/1‑150 (MQ=255)                 ttGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAacaaca                                                                                                                              <  2:1438683/150‑1 (MQ=255)                          cAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTccc                                                                                                                     >  1:80760/1‑150 (MQ=255)                           aGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTcccc                                                                                                                    <  1:1240919/150‑1 (MQ=255)                            gTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCct                                                                                                                   >  2:286884/1‑150 (MQ=255)                                ggTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCctgctg                                                                                                               >  1:15210/1‑150 (MQ=255)                                ggTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCctgctg                                                                                                               >  2:367866/1‑150 (MQ=255)                                   gtAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCtggtg                                                                                                            >  2:977117/1‑150 (MQ=255)                                    tAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCtggtgg                                                                                                           <  2:1240382/150‑1 (MQ=255)                                         cGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACAt                                                                                                      >  1:496093/1‑150 (MQ=255)                                          gggAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACAtt                                                                                                     >  2:381317/1‑150 (MQ=255)                                              aaCGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCg                                                                                                 <  1:286884/150‑1 (MQ=255)                                                cgcgGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGct                                                                                               >  1:545367/1‑150 (MQ=255)                                                   gggATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGctctc                                                                                            <  2:771392/150‑1 (MQ=255)                                                         aGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTcc                                                                                      >  1:1519669/1‑150 (MQ=255)                                                          gTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCt                                                                                     <  1:1189344/150‑1 (MQ=255)                                                            tAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCtga                                                                                   >  1:777381/1‑150 (MQ=255)                                                             aaTGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCtgat                                                                                  >  2:737901/1‑150 (MQ=255)                                                               tGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCtgatga                                                                                >  1:259895/1‑150 (MQ=255)                                                                gTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTgatgat                                                                               <  2:459811/150‑1 (MQ=255)                                                                    aCCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAg                                                                           <  2:1390294/150‑1 (MQ=255)                                                                    aCCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAg                                                                           <  1:161139/150‑1 (MQ=255)                                                                    aCCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAg                                                                           >  2:1001425/1‑150 (MQ=255)                                                                        gAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAg                                                                       >  1:373469/1‑150 (MQ=255)                                                                              gACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTTGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATcgc                                                                 >  2:797615/1‑150 (MQ=255)                                                                              gACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTTGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATcgc                                                                 >  2:791736/1‑150 (MQ=255)                                                                                cGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATcgcgc                                                               <  1:1017637/150‑1 (MQ=255)                                                                                 ggCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATcgcgcg                                                              <  1:513554/150‑1 (MQ=255)                                                                                     aCCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGACAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCgaatg                                                          <  2:1196281/150‑1 (MQ=255)                                                                                      ccACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCgaatga                                                         >  1:1271059/1‑150 (MQ=255)                                                                                      ccACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCgaatga                                                         >  2:534458/1‑150 (MQ=255)                                                                                        acaGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATcgcgcg                                                              >  1:1046516/1‑143 (MQ=255)                                                                                        acaGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATcgcgcg                                                              <  2:1046516/143‑1 (MQ=255)                                                                                           gACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTc                                                    >  1:1218109/1‑150 (MQ=255)                                                                                              cccAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTAt                                                 >  1:1372163/1‑150 (MQ=255)                                                                                                  gCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAg                                             >  2:199649/1‑150 (MQ=255)                                                                                                    tATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTc                                           <  1:1257156/150‑1 (MQ=255)                                                                                                      tCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTTGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAg                                         <  1:797615/150‑1 (MQ=255)                                                                                                      tCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTTGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAg                                         <  1:791736/150‑1 (MQ=255)                                                                                                       ccccAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGc                                        >  2:1163026/1‑150 (MQ=255)                                                                                                       ccccAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTACTGATGATGCAGCCAGTATCGCGCGAATGAATGTCTATGCAGTGAGc                                        >  1:519031/1‑150 (MQ=255)                                                                                                        cccAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCa                                       >  1:1242858/1‑150 (MQ=255)                                                                                                         ccAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCAc                                      <  2:373469/150‑1 (MQ=255)                                                                                                          cAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACg                                     >  1:338685/1‑150 (MQ=255)                                                                                                            ggCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGAc                                   <  1:977117/150‑1 (MQ=255)                                                                                                             gCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACa                                  <  1:660564/150‑1 (MQ=255)                                                                                                               gATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCgaa                                                            <  1:100797/122‑1 (MQ=255)                                                                                                               gATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCgaa                                                            >  2:100797/1‑122 (MQ=255)                                                                                                               gATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAgg                                <  2:924756/150‑1 (MQ=255)                                                                                                                 ttAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCATTCAGCACGACAGgtg                              <  2:1218109/150‑1 (MQ=255)                                                                                                                  tAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGgtgt                             <  2:259132/150‑1 (MQ=255)                                                                                                                    aGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCg                                                                                                 >  1:1491115/1‑80 (MQ=255)                                                                                                                    aGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCg                                                                                                 <  2:1491115/80‑1 (MQ=255)                                                                                                                    aGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCg                                                                                                 >  1:714374/1‑80 (MQ=255)                                                                                                                    aGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCg                                                                                                 <  2:714374/80‑1 (MQ=255)                                                                                                                     gACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTacc                          <  2:15210/150‑1 (MQ=255)                                                                                                                     gACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTacc                          >  1:7973/1‑150 (MQ=255)                                                                                                                        gtcacgtcCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTaccacc                       <  1:199649/150‑1 (MQ=255)                                                                                                                            cgtcCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCAATCCAGTCAGCACGACAGGTGTACCACCAGaa                   >  2:23375/1‑150 (MQ=255)                                                                                                                                cTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCg               <  1:737901/150‑1 (MQ=255)                                                                                                                                  ggcgtgggcgtgGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGTGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCggg             >  2:16587/1‑150 (MQ=255)                                                                                                                                  ggcgtgggcgtgGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCggg             <  1:367866/150‑1 (MQ=255)                                                                                                                                   gcgtgggcgtggTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGc            >  1:1459437/1‑150 (MQ=255)                                                                                                                                      tgggcgtggTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGAt         >  2:1002514/1‑150 (MQ=255)                                                                                                                                        ggcgtggTGGTGAACGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGAGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGCCCAttt       >  2:1091147/1‑150 (MQ=255)                                                                                                                                          cgtggTGGTGACCTAAATCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATACCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAACCCGGGCGATTTGa     <  2:1519669/150‑1 (MQ=255)                                                                                                                                             ggtggtgACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGATTTGAAGc  >  2:86012/1‑150 (MQ=255)                                                                                                                                                |                                                                                                                                                   GCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTCCTGGCGTGGGCGTGGTGGTGACCGATAGCCAGAACAACATTATTTCCCCTGCTGGTGGAACATTACCGCTCTCTATTCCTGATGATGCAGACAGTATCGCGCGAATGAATGTCTATCCAGTCAGCACGACAGGTGTACCACCAGAAACCGGGCGATTTGAAGC  >  NC_000913/1002557‑1002846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑