BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	134,169	A→G	9.1%	Y852C (TAC→TGC) ‡	acnB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	134,169	0	A	G	9.1%	312.7 / 16.0	109	Y852C (TAC→TGC) ‡	acnB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (52/47); new base G (8/2); total (60/49)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 110 total aligned reads displayed.

TGTCTTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCC  >  NC_000913/134020‑134315

tGTCTTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGACGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTa                                                                                                                                                     <  1:1402900/150‑1 (MQ=255)

    ttCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACAcc                                                                                                                                                 <  1:839957/150‑1 (MQ=255)

    ttCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACAcc                                                                                                                                                 >  2:1170190/1‑150 (MQ=255)

    ttCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACAcc                                                                                                                                                 <  1:6481/150‑1 (MQ=255)

     tCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCg                                                                                                                                                <  2:1123500/150‑1 (MQ=255)

      ccTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCga                                                                                                                                               >  2:1078196/1‑150 (MQ=255)

        tGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCgaga                                                                                                                                             >  1:1040606/1‑150 (MQ=255)

             tCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCGGTGTACTGAGAAAGct                                                                                                                                        <  2:84533/150‑2 (MQ=255)

              cTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCg                                                                                                                                       >  2:1051955/1‑150 (MQ=255)

                gCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCGGTGTACTGAGAAAGctggt                                                                                                                                     <  1:623911/150‑5 (MQ=255)

                gCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGAt                                                                                                                                     >  2:877070/1‑150 (MQ=255)

                gCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGAt                                                                                                                                     >  1:1534901/1‑150 (MQ=255)

                 cGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATg                                                                                                                                    <  1:1278766/150‑1 (MQ=255)

                    aaCTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATgggg                                                                                                                                 <  1:1286574/150‑1 (MQ=255)

                     aCTGGCGGCGGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGt                                                                                                                                <  2:1615474/150‑1 (MQ=255)

                        ggcggcTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGAt                                                                                                                             <  1:827240/150‑1 (MQ=255)

                              tGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCa                                                                                                                       <  1:87372/150‑1 (MQ=255)

                              tGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCa                                                                                                                       <  1:216230/150‑1 (MQ=255)

                               gTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCATCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAg                                                                                                                      <  2:1530974/150‑1 (MQ=255)

                               gTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAg                                                                                                                      <  1:697794/150‑1 (MQ=255)

                               gTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAg                                                                                                                      <  1:1193364/150‑1 (MQ=255)

                                    ggcgCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGc                                                                                                                 <  2:303598/150‑1 (MQ=255)

                                      cgcTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCgg                                                                                                               <  1:909481/150‑1 (MQ=255)

                                            ttGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAg                                                                                                                      >  2:292132/1‑137 (MQ=255)

                                            ttGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAg                                                                                                                      <  1:292132/137‑1 (MQ=255)

                                             tGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTaaaa                                                                                                        >  1:761066/1‑150 (MQ=255)

                                                  aaCTGGCGACGTCGTAAGAGTACCAGACCTACGGGGCGCAGGTTGATATATGAGCCGTTGATACTTTCCGTTATCTGATCTTCATGCAGGTTTCTCAGTACACGGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCa                                                                                                   <  2:521662/150‑1 (MQ=255)

                                                    cTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAgc                                                                                                 >  1:142073/1‑150 (MQ=255)

                                                    cTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAgc                                                                                                 <  2:1027010/150‑1 (MQ=255)

                                                     tGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAgcg                                                                                                <  2:1266240/150‑1 (MQ=255)

                                                        cGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCAc                                                                                             <  1:1074681/150‑1 (MQ=255)

                                                            gCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACgcgc                                                                                         <  2:755620/150‑1 (MQ=255)

                                                              cGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTTTCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTg                                                                                       >  2:1446324/1‑150 (MQ=255)

                                                              cGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTg                                                                                       >  1:1054770/1‑150 (MQ=255)

                                                                gAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTgcg                                                                                     <  1:1170190/150‑1 (MQ=255)

                                                                   gagTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCAt                                                                                  <  2:1568198/150‑1 (MQ=255)

                                                                    agTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATa                                                                                 >  2:1546652/1‑150 (MQ=255)

                                                                     gTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATaa                                                                                >  1:801605/1‑150 (MQ=255)

                                                                      tACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGTTACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATaaa                                                                               <  2:1548190/150‑1 (MQ=255)

                                                                        ccAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACg                                                                             >  2:1568822/1‑150 (MQ=255)

                                                                         cAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCAGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGa                                                                            >  1:1153458/1‑150 (MQ=255)

                                                                          aGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGac                                                                           >  2:430894/1‑150 (MQ=255)

                                                                            aCCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGacac                                                                         >  1:1165767/1‑150 (MQ=255)

                                                                            aCCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGacac                                                                         >  1:282672/1‑150 (MQ=255)

                                                                               tACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAAt                                                                      >  1:463774/1‑150 (MQ=255)

                                                                                    ggCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCg                                                                 <  1:1051955/150‑1 (MQ=255)

                                                                                      cgcAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACAGCTATAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCTCGCGCTGCGCATAAACGACACAATGCCCGGt                                                               <  1:699774/150‑1 (MQ=255)

                                                                                      cgcAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGt                                                               >  2:177261/1‑150 (MQ=255)

                                                                                      cgcAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGt                                                               <  1:1511389/150‑1 (MQ=255)

                                                                                          ggTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAAt                                                           >  2:1393776/1‑150 (MQ=255)

                                                                                             aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                        >  2:1243178/1‑150 (MQ=255)

                                                                                             aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                        >  1:1080298/1‑150 (MQ=255)

                                                                                             aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                        >  1:953610/1‑150 (MQ=255)

                                                                                             aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                        <  2:1165767/150‑1 (MQ=255)

                                                                                             aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTACAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                        >  2:1369274/1‑150 (MQ=255)

                                                                                             aGATAAAACAGACGTTGATACTTACCGTTATCTGAAATTCAACCAGCTTTCTCAGTACACAGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATTCCCGGTGAATgag                                                        >  2:1590474/1‑150 (MQ=255)

                                                                                                tAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGAtt                                                     >  2:612542/1‑150 (MQ=255)

                                                                                                   aaCAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGTGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTc‑cc                                                 <  2:344843/150‑1 (MQ=255)

                                                                                                             gATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCAttttt                                       <  1:877070/150‑1 (MQ=255)

                                                                                                               tACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCAttttttt                                     >  1:297847/1‑150 (MQ=255)

                                                                                                               tACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGAGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCAttttttt                                     >  2:974976/1‑150 (MQ=255)

                                                                                                                 cTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCAGGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTAt                                   <  1:343391/150‑1 (MQ=255)

                                                                                                                   tACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTAttt                                 >  2:493394/1‑150 (MQ=255)

                                                                                                                   tACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTAttt                                 >  1:854971/1‑150 (MQ=255)

                                                                                                                    aCCGTTATCTGAACTACAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTc                                <  1:1369274/150‑1 (MQ=255)

                                                                                                                     ccGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCt                               <  1:430894/150‑1 (MQ=255)

                                                                                                                     ccGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGA‑TTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTa                              >  1:525859/1‑150 (MQ=255)

                                                                                                                         tATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAAc                           >  2:1424051/1‑150 (MQ=255)

                                                                                                                           tCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCa                         >  2:1416156/1‑150 (MQ=255)

                                                                                                                            cTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCAt                        >  2:1135667/1‑150 (MQ=255)

                                                                                                                               aaCTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGc                     <  1:855407/150‑1 (MQ=255)

                                                                                                                                 cTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCg                   >  1:34778/1‑150 (MQ=255)

                                                                                                                                 cTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCg                   >  2:50539/1‑150 (MQ=255)

                                                                                                                                   cccatcggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGtt                 >  2:843384/8‑150 (MQ=255)

                                                                                                                                    cAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTc                >  2:1410225/1‑150 (MQ=255)

                                                                                                                                     aaCCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTc‑c                                                  >  1:679391/1‑115 (MQ=255)

                                                                                                                                      atcggCTTTCTCGGTGTACTGAGAAAGCCGATGCGGTGAAATTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCg              >  2:832554/5‑150 (MQ=255)

                                                                                                                                       ccAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCgg                                                                >  2:936327/1‑100 (MQ=255)

                                                                                                                                       ccAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCgg                                                                <  1:936327/100‑1 (MQ=255)

                                                                                                                                       ccAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGc             <  2:801605/150‑1 (MQ=255)

                                                                                                                                        cAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCt            <  2:761066/150‑1 (MQ=255)

                                                                                                                                         ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCTCGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg           >  1:86832/2‑150 (MQ=255)

                                                                                                                                         ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg           >  2:1591432/2‑150 (MQ=255)

                                                                                                                                         ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg           >  1:69721/2‑150 (MQ=255)

                                                                                                                                         ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg           >  1:502296/2‑150 (MQ=255)

                                                                                                                                         ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg           >  1:86830/2‑150 (MQ=255)

                                                                                                                                         aGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg           <  1:1568822/150‑1 (MQ=255)

                                                                                                                                          tcTTTCTCAGTACACCGATAAAGCCGATGGGGTGATTTTCCAGACTGCGTTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCTGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGt          <  2:1153458/149‑1 (MQ=255)

                                                                                                                                            tttCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGttt        <  1:1078196/150‑1 (MQ=255)

                                                                                                                                            tttCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTATAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGCGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGttt        >  2:1444935/1‑150 (MQ=255)

                                                                                                                                              tctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGttttt      >  1:1546110/1‑150 (MQ=255)

                                                                                                                                               ctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATTCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtc     <  2:282672/150‑1 (MQ=255)

                                                                                                                                               ctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtc     <  2:953610/150‑1 (MQ=255)

                                                                                                                                               ctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtc     >  1:635611/1‑150 (MQ=255)

                                                                                                                                                tcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtct    >  2:1123030/1‑150 (MQ=255)

                                                                                                                                                tcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGG‑GAATCTCATTCACCGGGCAttgtgt                                      <  1:1598010/116‑5 (MQ=37)

                                                                                                                                                tcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGG‑GAATCTCATTCACCGGGCAttgtgt                                      >  2:1598010/1‑112 (MQ=37)

                                                                                                                                                 cAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCGGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtctc   >  2:637988/1‑150 (MQ=255)

                                                                                                                                                 cAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtctc   >  2:1372971/1‑150 (MQ=255)

                                                                                                                                                  aGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTcc  >  2:1120411/1‑150 (MQ=255)

TGTCTTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCC  >  NC_000913/134020‑134315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`