BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	134,170	C→T	9.2%	Y852Y (TAC→TAT) ‡	acnB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	134,170	0	C	T	9.2%	322.4 / 15.8	109	Y852Y (TAC→TAT) ‡	acnB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (52/47); new base T (8/2); total (60/49)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

Only 100 of 112 total aligned reads displayed.

TTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTA  >  NC_000913/134024‑134319

ttCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACAcc                                                                                                                                                     <  1:839957/150‑1 (MQ=255)

ttCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACAcc                                                                                                                                                     <  1:6481/150‑1 (MQ=255)

 tCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCg                                                                                                                                                    <  2:1123500/150‑1 (MQ=255)

  ccTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCga                                                                                                                                                   >  2:1078196/1‑150 (MQ=255)

    tGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCgaga                                                                                                                                                 >  1:1040606/1‑150 (MQ=255)

         tCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCGGTGTACTGAGAAAGct                                                                                                                                            <  2:84533/150‑2 (MQ=255)

          cTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCg                                                                                                                                           >  2:1051955/1‑150 (MQ=255)

            gCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCGGTGTACTGAGAAAGctggt                                                                                                                                         <  1:623911/150‑5 (MQ=255)

            gCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGAt                                                                                                                                         >  2:877070/1‑150 (MQ=255)

            gCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGAt                                                                                                                                         >  1:1534901/1‑150 (MQ=255)

             cGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATg                                                                                                                                        <  1:1278766/150‑1 (MQ=255)

                aaCTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATgggg                                                                                                                                     <  1:1286574/150‑1 (MQ=255)

                 aCTGGCGGCGGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGt                                                                                                                                    <  2:1615474/150‑1 (MQ=255)

                    ggcggcTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGAt                                                                                                                                 <  1:827240/150‑1 (MQ=255)

                          tGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCa                                                                                                                           <  1:87372/150‑1 (MQ=255)

                          tGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCa                                                                                                                           <  1:216230/150‑1 (MQ=255)

                           gTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCATCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAg                                                                                                                          <  2:1530974/150‑1 (MQ=255)

                           gTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAg                                                                                                                          <  1:697794/150‑1 (MQ=255)

                                ggcgCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGc                                                                                                                     <  2:303598/150‑1 (MQ=255)

                                  cgcTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCgg                                                                                                                   <  1:909481/150‑1 (MQ=255)

                                        ttGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAg                                                                                                                          >  2:292132/1‑137 (MQ=255)

                                        ttGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAg                                                                                                                          <  1:292132/137‑1 (MQ=255)

                                         tGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTaaaa                                                                                                            >  1:761066/1‑150 (MQ=255)

                                            cAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGt                                                                                                         >  2:1511389/1‑150 (MQ=255)

                                              aaCTGGCGACGTCGTAAGAGTACCAGACCTACGGGGCGCAGGTTGATATATGAGCCGTTGATACTTTCCGTTATCTGATCTTCATGCAGGTTTCTCAGTACACGGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCa                                                                                                       <  2:521662/150‑1 (MQ=255)

                                                cTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAgc                                                                                                     >  1:142073/1‑150 (MQ=255)

                                                cTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAgc                                                                                                     <  2:1027010/150‑1 (MQ=255)

                                                 tGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAgcg                                                                                                    <  2:1266240/150‑1 (MQ=255)

                                                    cGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCAc                                                                                                 <  1:1074681/150‑1 (MQ=255)

                                                        gCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACgcgc                                                                                             <  2:755620/150‑1 (MQ=255)

                                                          cGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTTTCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTg                                                                                           >  2:1446324/1‑150 (MQ=255)

                                                          cGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTg                                                                                           >  1:1054770/1‑150 (MQ=255)

                                                            gAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTgcg                                                                                         <  1:1170190/150‑1 (MQ=255)

                                                               gagTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCAt                                                                                      <  2:1568198/150‑1 (MQ=255)

                                                                agTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATa                                                                                     >  2:1546652/1‑150 (MQ=255)

                                                                 gTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATaa                                                                                    >  1:801605/1‑150 (MQ=255)

                                                                  tACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGTTACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATaaa                                                                                   <  2:1548190/150‑1 (MQ=255)

                                                                     cAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCAGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGa                                                                                >  1:1153458/1‑150 (MQ=255)

                                                                      aGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGac                                                                               >  2:430894/1‑150 (MQ=255)

                                                                        aCCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGacac                                                                             >  1:1165767/1‑150 (MQ=255)

                                                                        aCCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGacac                                                                             >  1:282672/1‑150 (MQ=255)

                                                                           tACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAAt                                                                          >  1:463774/1‑150 (MQ=255)

                                                                                ggCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCg                                                                     <  1:1051955/150‑1 (MQ=255)

                                                                                 gcgcAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCgg                                                                    <  2:202310/150‑1 (MQ=255)

                                                                                  cgcAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACAGCTATAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCTCGCGCTGCGCATAAACGACACAATGCCCGGt                                                                   <  1:699774/150‑1 (MQ=255)

                                                                                  cgcAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGt                                                                   >  2:177261/1‑150 (MQ=255)

                                                                                  cgcAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGt                                                                   <  1:1511389/150‑1 (MQ=255)

                                                                                      ggTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAAt                                                               >  2:1393776/1‑150 (MQ=255)

                                                                                         aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                            >  1:1080298/1‑150 (MQ=255)

                                                                                         aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                            >  2:1243178/1‑150 (MQ=255)

                                                                                         aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                            <  2:1165767/150‑1 (MQ=255)

                                                                                         aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                            >  1:953610/1‑150 (MQ=255)

                                                                                         aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTACAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                            >  2:1369274/1‑150 (MQ=255)

                                                                                         aGATAAAACAGACGTTGATACTTACCGTTATCTGAAATTCAACCAGCTTTCTCAGTACACAGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATTCCCGGTGAATgag                                                            >  2:1590474/1‑150 (MQ=255)

                                                                                            tAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGAtt                                                         >  2:612542/1‑150 (MQ=255)

                                                                                            tAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGAtt                                                         >  1:827452/1‑150 (MQ=255)

                                                                                                         gATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCAttttt                                           <  1:877070/150‑1 (MQ=255)

                                                                                                           tACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCAttttttt                                         >  1:297847/1‑150 (MQ=255)

                                                                                                           tACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGAGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCAttttttt                                         >  2:974976/1‑150 (MQ=255)

                                                                                                             cTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCAGGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTAt                                       <  1:343391/150‑1 (MQ=255)

                                                                                                               tACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTAttt                                     >  2:493394/1‑150 (MQ=255)

                                                                                                                aCCGTTATCTGAACTACAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTc                                    <  1:1369274/150‑1 (MQ=255)

                                                                                                                 ccGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCt                                   <  1:430894/150‑1 (MQ=255)

                                                                                                                 ccGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGA‑TTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTa                                  >  1:531297/1‑150 (MQ=255)

                                                                                                                 ccGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGA‑TTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTa                                  >  1:525859/1‑150 (MQ=255)

                                                                                                                       tCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCa                             >  2:1416156/1‑150 (MQ=255)

                                                                                                                       tCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCa                             <  1:1607470/150‑1 (MQ=255)

                                                                                                                        cTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCAt                            >  2:1135667/1‑150 (MQ=255)

                                                                                                                           aaCTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGc                         <  1:855407/150‑1 (MQ=255)

                                                                                                                             cTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCg                       >  2:50539/1‑150 (MQ=255)

                                                                                                                               cccatcggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGtt                     >  2:843384/8‑150 (MQ=255)

                                                                                                                                cAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTc                    >  2:1410225/1‑150 (MQ=255)

                                                                                                                                 aaCCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTc‑c                                                      <  2:679391/115‑1 (MQ=255)

                                                                                                                                 aaCCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTcc                   >  2:1238008/1‑150 (MQ=255)

                                                                                                                                  atcggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCg                  >  1:1217453/5‑150 (MQ=255)

                                                                                                                                  atcggCTTTCTCGGTGTACTGAGAAAGCCGATGCGGTGAAATTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCg                  >  2:832554/5‑150 (MQ=255)

                                                                                                                                   ccAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCgg                                                                    >  2:936327/1‑100 (MQ=255)

                                                                                                                                   ccAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCgg                                                                    <  1:936327/100‑1 (MQ=255)

                                                                                                                                   ccAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGc                 <  2:801605/150‑1 (MQ=255)

                                                                                                                                     ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCTCGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg               >  1:86832/2‑150 (MQ=255)

                                                                                                                                     ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg               >  1:69721/2‑150 (MQ=255)

                                                                                                                                     ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg               >  1:502296/2‑150 (MQ=255)

                                                                                                                                     ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg               >  1:86830/2‑150 (MQ=255)

                                                                                                                                     ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg               >  2:1591432/2‑150 (MQ=255)

                                                                                                                                     aGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg               <  1:1568822/150‑1 (MQ=255)

                                                                                                                                      tcTTTCTCAGTACACCGATAAAGCCGATGGGGTGATTTTCCAGACTGCGTTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCTGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGt              <  2:1153458/149‑1 (MQ=255)

                                                                                                                                        tttCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGttt            <  1:1078196/150‑1 (MQ=255)

                                                                                                                                        tttCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTATAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGCGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGttt            >  2:1444935/1‑150 (MQ=255)

                                                                                                                                           ctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATTCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtc         <  2:282672/150‑1 (MQ=255)

                                                                                                                                           ctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtc         <  2:953610/150‑1 (MQ=255)

                                                                                                                                           ctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtc         >  1:635611/1‑150 (MQ=255)

                                                                                                                                            tcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtct        >  2:1123030/1‑150 (MQ=255)

                                                                                                                                            tcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGG‑GAATCTCATTCACCGGGCAttgtgt                                          >  2:1598010/1‑112 (MQ=37)

                                                                                                                                            tcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGG‑GAATCTCATTCACCGGGCAttgtgt                                          <  1:1598010/116‑5 (MQ=37)

                                                                                                                                             cAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCGGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtctc       >  2:637988/1‑150 (MQ=255)

                                                                                                                                             cAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtctc       >  2:1372971/1‑150 (MQ=255)

                                                                                                                                              aGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTcc      >  2:1120411/1‑150 (MQ=255)

                                                                                                                                                  cacCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTa  <  1:612542/150‑1 (MQ=255)

                                                                                                                                                  cacCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTa  <  1:1446324/150‑1 (MQ=255)

                                                                                                                                                  cacCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTa  >  2:1434619/1‑150 (MQ=255)

TTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTA  >  NC_000913/134024‑134319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`