Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 134,173 C→T 9.2% T853T (ACC→ACT)  acnB → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913134,1730CT 9.2% 316.9 / 15.9 110T853T (ACC→ACT) acnBhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (52/46);  new base T (8/2);  total (61/49)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.37e-01

Only 100 of 115 total aligned reads displayed.

TTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGG  >  NC_000913/134024‑134322
                                                                                                                                                     |                                                                                                                                                      
ttCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACAcc                                                                                                                                                        >  2:1170190/1‑150 (MQ=255)
ttCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACAcc                                                                                                                                                        <  1:839957/150‑1 (MQ=255)
 tCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCg                                                                                                                                                       <  2:1123500/150‑1 (MQ=255)
  ccTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCga                                                                                                                                                      >  2:1078196/1‑150 (MQ=255)
    tGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCgaga                                                                                                                                                    >  1:1040606/1‑150 (MQ=255)
         tCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCGGTGTACTGAGAAAGct                                                                                                                                               <  2:84533/150‑2 (MQ=255)
          cTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCg                                                                                                                                              >  2:1051955/1‑150 (MQ=255)
            gCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCGGTGTACTGAGAAAGctggt                                                                                                                                            <  1:623911/150‑5 (MQ=255)
            gCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGAt                                                                                                                                            >  2:877070/1‑150 (MQ=255)
            gCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGAt                                                                                                                                            >  1:1534901/1‑150 (MQ=255)
             cGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATg                                                                                                                                           <  1:1278766/150‑1 (MQ=255)
                aaCTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATgggg                                                                                                                                        <  1:1286574/150‑1 (MQ=255)
                 aCTGGCGGCGGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGt                                                                                                                                       <  2:1615474/150‑1 (MQ=255)
                          tGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCa                                                                                                                              <  1:87372/150‑1 (MQ=255)
                          tGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCa                                                                                                                              <  1:216230/150‑1 (MQ=255)
                           gTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCATCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAg                                                                                                                             <  2:1530974/150‑1 (MQ=255)
                           gTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAg                                                                                                                             <  1:697794/150‑1 (MQ=255)
                           gTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAg                                                                                                                             <  1:1193364/150‑1 (MQ=255)
                                ggcgCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGc                                                                                                                        <  2:303598/150‑1 (MQ=255)
                                  cgcTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCgg                                                                                                                      <  1:909481/150‑1 (MQ=255)
                                        ttGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAg                                                                                                                             >  2:292132/1‑137 (MQ=255)
                                        ttGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAg                                                                                                                             <  1:292132/137‑1 (MQ=255)
                                         tGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTaaaa                                                                                                               >  1:761066/1‑150 (MQ=255)
                                            cAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGt                                                                                                            >  2:1511389/1‑150 (MQ=255)
                                              aaCTGGCGACGTCGTAAGAGTACCAGACCTACGGGGCGCAGGTTGATATATGAGCCGTTGATACTTTCCGTTATCTGATCTTCATGCAGGTTTCTCAGTACACGGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCa                                                                                                          <  2:521662/150‑1 (MQ=255)
                                                cTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAgc                                                                                                        <  2:1027010/150‑1 (MQ=255)
                                                 tGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAgcg                                                                                                       <  2:1266240/150‑1 (MQ=255)
                                                    cGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCAc                                                                                                    <  1:1074681/150‑1 (MQ=255)
                                                        gCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACgcgc                                                                                                <  2:755620/150‑1 (MQ=255)
                                                          cGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTTTCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTg                                                                                              >  2:1446324/1‑150 (MQ=255)
                                                          cGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTg                                                                                              >  1:1054770/1‑150 (MQ=255)
                                                            gAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTgcg                                                                                            <  1:1170190/150‑1 (MQ=255)
                                                               gagTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCAt                                                                                         <  2:1568198/150‑1 (MQ=255)
                                                                agTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATa                                                                                        >  2:1546652/1‑150 (MQ=255)
                                                                 gTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATaa                                                                                       >  1:801605/1‑150 (MQ=255)
                                                                    ccAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACg                                                                                    >  2:1568822/1‑150 (MQ=255)
                                                                     cAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCAGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGa                                                                                   >  1:1153458/1‑150 (MQ=255)
                                                                      aGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGac                                                                                  >  2:430894/1‑150 (MQ=255)
                                                                        aCCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGacac                                                                                >  1:282672/1‑150 (MQ=255)
                                                                           tACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAAt                                                                             >  1:463774/1‑150 (MQ=255)
                                                                                ggCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCg                                                                        <  1:1051955/150‑1 (MQ=255)
                                                                                 gcgcAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCgg                                                                       <  2:202310/150‑1 (MQ=255)
                                                                                  cgcAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACAGCTATAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCTCGCGCTGCGCATAAACGACACAATGCCCGGt                                                                      <  1:699774/150‑1 (MQ=255)
                                                                                  cgcAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGt                                                                      <  1:1511389/150‑1 (MQ=255)
                                                                                      ggTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAAt                                                                  >  2:1393776/1‑150 (MQ=255)
                                                                                         aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                               >  2:1243178/1‑150 (MQ=255)
                                                                                         aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                               >  1:953610/1‑150 (MQ=255)
                                                                                         aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                               >  1:1080298/1‑150 (MQ=255)
                                                                                         aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                               <  2:1165767/150‑1 (MQ=255)
                                                                                         aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTACAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                               >  2:1369274/1‑150 (MQ=255)
                                                                                         aGATAAAACAGACGTTGATACTTACCGTTATCTGAAATTCAACCAGCTTTCTCAGTACACAGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATTCCCGGTGAATgag                                                               >  2:1590474/1‑150 (MQ=255)
                                                                                            tAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGAtt                                                            >  1:827452/1‑150 (MQ=255)
                                                                                            tAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGAtt                                                            >  2:612542/1‑150 (MQ=255)
                                                                                               aaCAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGTGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTc‑cc                                                        <  2:344843/150‑1 (MQ=255)
                                                                                                           tACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCAttttttt                                            >  1:297847/1‑150 (MQ=255)
                                                                                                           tACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGAGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCAttttttt                                            >  2:974976/1‑150 (MQ=255)
                                                                                                             cTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCAGGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTAt                                          <  1:343391/150‑1 (MQ=255)
                                                                                                             cTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTAt                                          <  1:995652/150‑1 (MQ=255)
                                                                                                               tACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTAttt                                        >  2:493394/1‑150 (MQ=255)
                                                                                                               tACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTAttt                                        >  1:854971/1‑150 (MQ=255)
                                                                                                                aCCGTTATCTGAACTACAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTc                                       <  1:1369274/150‑1 (MQ=255)
                                                                                                                 ccGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGA‑TTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTa                                     >  1:525859/1‑150 (MQ=255)
                                                                                                                 ccGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGA‑TTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTa                                     >  1:531297/1‑150 (MQ=255)
                                                                                                                       tCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCa                                >  2:1416156/1‑150 (MQ=255)
                                                                                                                        cTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCAt                               >  2:1135667/1‑150 (MQ=255)
                                                                                                                           aaCTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGc                            <  1:855407/150‑1 (MQ=255)
                                                                                                                             cTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCg                          >  1:34778/1‑150 (MQ=255)
                                                                                                                               cccatcggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGtt                        >  2:843384/8‑150 (MQ=255)
                                                                                                                                cAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTc                       >  2:1410225/1‑150 (MQ=255)
                                                                                                                                 aaCCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTc‑c                                                         <  2:679391/115‑1 (MQ=255)
                                                                                                                                 aaCCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTc‑c                                                         >  1:679391/1‑115 (MQ=255)
                                                                                                                                  atcggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCg                     >  1:1217453/5‑150 (MQ=255)
                                                                                                                                   ccAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCgg                                                                       >  2:936327/1‑100 (MQ=255)
                                                                                                                                   ccAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCgg                                                                       <  1:936327/100‑1 (MQ=255)
                                                                                                                                   ccAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGc                    <  2:801605/150‑1 (MQ=255)
                                                                                                                                    cAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCt                   <  2:761066/150‑1 (MQ=255)
                                                                                                                                     ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCTCGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg                  >  1:86832/2‑150 (MQ=255)
                                                                                                                                     ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg                  >  2:1591432/2‑150 (MQ=255)
                                                                                                                                     ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg                  >  1:69721/2‑150 (MQ=255)
                                                                                                                                     ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg                  >  1:502296/2‑150 (MQ=255)
                                                                                                                                     ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg                  >  1:86830/2‑150 (MQ=255)
                                                                                                                                     aGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg                  <  1:1568822/150‑1 (MQ=255)
                                                                                                                                      tcTTTCTCAGTACACCGATAAAGCCGATGGGGTGATTTTCCAGACTGCGTTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCTGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGt                 <  2:1153458/149‑1 (MQ=255)
                                                                                                                                        tttCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGttt               <  1:1078196/150‑1 (MQ=255)
                                                                                                                                        tttCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTATAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGCGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGttt               >  2:1444935/1‑150 (MQ=255)
                                                                                                                                          tctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGttttt             >  1:1546110/1‑150 (MQ=255)
                                                                                                                                           ctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATTCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtc            <  2:282672/150‑1 (MQ=255)
                                                                                                                                           ctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtc            >  1:635611/1‑150 (MQ=255)
                                                                                                                                           ctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtc            <  2:953610/150‑1 (MQ=255)
                                                                                                                                            tcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtct           >  2:1123030/1‑150 (MQ=255)
                                                                                                                                            tcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGG‑GAATCTCATTCACCGGGCAttgtgt                                             <  1:1598010/116‑5 (MQ=37)
                                                                                                                                            tcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGG‑GAATCTCATTCACCGGGCAttgtgt                                             >  2:1598010/1‑112 (MQ=37)
                                                                                                                                             cAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCGGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtctc          >  2:637988/1‑150 (MQ=255)
                                                                                                                                             cAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtctc          >  2:1372971/1‑150 (MQ=255)
                                                                                                                                                  cacCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTa     <  1:1446324/150‑1 (MQ=255)
                                                                                                                                                  cacCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTa     <  1:612542/150‑1 (MQ=255)
                                                                                                                                                  cacCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTa     >  2:1434619/1‑150 (MQ=255)
                                                                                                                                                   acCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTaa    >  1:11854/1‑150 (MQ=255)
                                                                                                                                                    ccGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAg   >  2:15453/1‑150 (MQ=255)
                                                                                                                                                     cGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAgg  >  1:266386/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGG  >  NC_000913/134024‑134322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑