Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,346,955 G→C 11.1% intergenic (+55/+23) ymiB → / ← rnb putative protein YmiB/RNase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,346,9550GC11.1% 302.3 / 27.2 108intergenic (+55/+23)ymiB/rnbputative protein YmiB/RNase II
Reads supporting (aligned to +/- strand):  ref base G (43/53);  new base C (8/4);  total (51/57)
Fisher's exact test for biased strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

Only 100 of 109 total aligned reads displayed.

GAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTG  >  NC_000913/1346807‑1347104
                                                                                                                                                    |                                                                                                                                                     
gAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGa                                                                                                                                                      >  1:1202684/1‑150 (MQ=255)
 aaaTTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAg                                                                                                                                                     <  2:1088135/150‑1 (MQ=255)
  aaTTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAgg                                                                                                                                                    <  2:125588/150‑1 (MQ=255)
   aTTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGa                                                                                                                                                   >  1:1567249/1‑150 (MQ=255)
       aCGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATgg                                                                                                                                               <  1:1033939/150‑1 (MQ=255)
        cggcggCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATggg                                                                                                                                              >  1:718769/1‑150 (MQ=255)
         ggcggcTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGc                                                                                                                                             >  1:531216/1‑150 (MQ=255)
         ggcggcTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGc                                                                                                                                             >  1:29109/1‑150 (MQ=255)
             gcTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATTCCTCATGGATGGGCCGtt                                                                                                                                         <  1:246633/150‑2 (MQ=255)
               tAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGaa                                                                                                                                       >  2:164221/1‑150 (MQ=255)
               aaaaCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTTGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATTCCTCATGGATGGGCCGttta                                                                                                                                       <  2:593986/149‑4 (MQ=255)
                aaaCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGTAAAACGCCAGTTTTCAAACGAAAGTCATTTAAAAAATGTTCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGaaa                                                                                                                                      <  2:559443/150‑1 (MQ=255)
                aaaCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATTCCTCATGGATGGGCCGtttat                                                                                                                                      <  2:1479961/150‑5 (MQ=255)
                 aaCGCATTGTTTTTTTTATATATTTATTTTTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAg                                                                                                                                     <  1:1091288/150‑1 (MQ=255)
                 aaCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATTCCTCATGGATGGGCCGtttatt                                                                                                                                     <  1:784871/150‑6 (MQ=255)
                 aaCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGACTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCAATCCATGAGGAATGGGCCTTCAAAg                                                                                                                                     >  2:801068/1‑150 (MQ=255)
                    gCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGgag                                                                                                                                  <  2:789616/150‑1 (MQ=255)
                    gCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGgag                                                                                                                                  <  1:449751/150‑1 (MQ=255)
                      aTTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGAt                                                                                                                                >  2:414168/1‑150 (MQ=255)
                        tGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTa                                                                                                                              >  2:597585/1‑150 (MQ=255)
                         gTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGAttgc                                                                                                                             >  1:702017/1‑148 (MQ=255)
                         gTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTAc                                                                                                                             >  2:1532333/1‑150 (MQ=255)
                           tttttttATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTAcgc                                                                                                                           <  2:173562/150‑1 (MQ=255)
                                ttATATATTTATTTGTAATCCAGTTTTGGAAAACCGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCGTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCg                                                                                                                      >  1:516772/1‑150 (MQ=255)
                                 tatataTTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCgg                                                                                                                     >  1:1306185/1‑150 (MQ=255)
                                   tatatTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGgc                                                                                                                   >  1:33591/1‑150 (MQ=255)
                                     tatttattTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGgcgc                                                                                                                 >  2:1287557/1‑150 (MQ=255)
                                     tatttattTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGgcgc                                                                                                                 >  1:1480982/1‑150 (MQ=255)
                                      atttatttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGgcgcg                                                                                                                <  2:1124548/150‑1 (MQ=255)
                                       tttatttTTAATACAGTATTGCAAAAAAGCAAGATTACAAACGAAAGTGAGTTAAAAAATCAGAATGGAAATAACGAAGTTAGATGGGGGAAATAAACGGCCAATCCATGAGGAATGGGACGTGAAATGAGATTACGCGACCGGgcgcgc                                                                                                               >  2:724015/1‑150 (MQ=255)
                                        ttatttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCTAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCa                                                                                                              <  1:1529838/150‑1 (MQ=255)
                                         tatttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAATTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGgaga                                                                                                                                 <  1:468425/130‑1 (MQ=255)
                                         tatttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAATTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGgaga                                                                                                                                 >  2:468425/1‑130 (MQ=255)
                                          atttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCaat                                                                                                            <  2:1086715/150‑1 (MQ=255)
                                             tGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCaataat                                                                                                         >  1:234277/1‑150 (MQ=255)
                                             tGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCaataat                                                                                                         >  2:210067/1‑150 (MQ=255)
                                                  tCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTAAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAAtgctgc                                                                                                    >  2:729174/1‑150 (MQ=255)
                                                   ccAGTTTTGGAAAAACGCCAGATTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCAATCCATGAGGAATGGGCCGAGAAAGTAGATTACGCGACCGGGCGCGCAATAATGCTGCg                                                                                                   >  2:315702/1‑150 (MQ=255)
                                                      gTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGt                                                                                                <  1:1450690/150‑1 (MQ=255)
                                                         ttGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTc                                                                                             <  1:1271381/150‑1 (MQ=255)
                                                         ttGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTc                                                                                             >  1:1272723/1‑150 (MQ=255)
                                                         ttGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGCGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTc                                                                                             >  2:1148516/1‑150 (MQ=255)
                                                           ggAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCa                                                                                           <  2:509109/150‑1 (MQ=255)
                                                           ggAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCa                                                                                           <  1:232665/150‑1 (MQ=255)
                                                              aaaaCGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGc                                                                                        <  1:307842/150‑1 (MQ=255)
                                                               aaaCGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCg                                                                                       <  2:703330/150‑1 (MQ=255)
                                                                aaCGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCgg                                                                                      >  1:589712/1‑150 (MQ=255)
                                                                  cGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGAc                                                                                    >  2:470388/1‑150 (MQ=255)
                                                                        ttttCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGCAGGTTTCGCGGGGAAATAAACGGCCCATCCATGCGGAATGGGCCGTGAAATGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGc                                                                              <  1:23436/150‑1 (MQ=255)
                                                                        ttttCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGc                                                                              <  2:531216/150‑1 (MQ=255)
                                                                           tCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCCGCGGGTTTCCATGCGGACTTCGGCAAt                                                                           <  1:148080/150‑1 (MQ=255)
                                                                            cAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATa                                                                          <  1:470388/150‑1 (MQ=255)
                                                                             aaaCGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAg                                                                         <  2:226789/150‑1 (MQ=255)
                                                                             aaaCGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAg                                                                         >  2:1019550/1‑150 (MQ=255)
                                                                                cGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGCGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGa                                                                      <  1:1148516/150‑1 (MQ=255)
                                                                                 gAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGAc                                                                     >  2:1251709/1‑150 (MQ=255)
                                                                                    aGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTc                                                                  <  2:25438/150‑1 (MQ=255)
                                                                                       cAGTTAATAAATCTGCCTGGTTATAACGAAGGTAGTGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCGGTGTATGGAGATTACGCGAGCGGGCGCGCAATATTGCTGCGGGTTTCCTTGCGGACTTCGGCAATAGTGACGTCAAt                                                               <  1:1166141/150‑1 (MQ=255)
                                                                                         gTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATaa                                                             >  2:819853/1‑150 (MQ=255)
                                                                                           tAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACg                                                           >  1:1421909/1‑150 (MQ=255)
                                                                                               aaaTCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGATTTCGGCAATAGTGACGTCAATAACgtcag                                                       <  1:804492/150‑1 (MQ=255)
                                                                                                  tCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACgtcagtca                                                    <  1:233797/150‑1 (MQ=255)
                                                                                                   cTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTTATTGACgtcactatt                                                   <  1:589727/150‑6 (MQ=255)
                                                                                                    tGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTTATTGACgtcactattg                                                  <  2:1508108/150‑7 (MQ=255)
                                                                                                     gCCTGCATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACtt                                                 <  1:183570/150‑1 (MQ=255)
                                                                                                       cTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGt                                                                        <  2:1151433/125‑1 (MQ=255)
                                                                                                       cTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGt                                                                        >  1:1151433/1‑125 (MQ=255)
                                                                                                          gATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTaa                                            >  1:1616392/1‑150 (MQ=255)
                                                                                                            tataACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAAc                                          >  1:821651/1‑150 (MQ=255)
                                                                                                             ataACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAAcc                                         <  1:144663/150‑1 (MQ=255)
                                                                                                                 cGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGttt                                     >  2:1455172/1‑150 (MQ=255)
                                                                                                                  gAAGTTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTc                                    <  2:1445784/150‑1 (MQ=255)
                                                                                                                  gAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTc                                    <  2:1567249/150‑1 (MQ=255)
                                                                                                                   aaGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCa                                   <  1:1522506/150‑1 (MQ=255)
                                                                                                                      gTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCt                                <  1:210067/150‑1 (MQ=255)
                                                                                                                          agCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTaa                            <  1:1245387/150‑1 (MQ=255)
                                                                                                                             ggggAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAAttt                         <  2:718769/150‑1 (MQ=255)
                                                                                                                              gggAAATAAACGGCCCATCCATGAGGAATGGGCCATGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATTCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTTTAAACCGTTTCACCTTTAAtttt                        >  2:462478/1‑149 (MQ=255)
                                                                                                                                gAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTa                      <  2:1593523/150‑1 (MQ=255)
                                                                                                                                 cctttcaCGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTAc                     >  1:1185619/7‑150 (MQ=255)
                                                                                                                                 aaaTAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCAcc                                 <  2:1561820/138‑1 (MQ=255)
                                                                                                                                 aaaTAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCAcc                                 >  1:1561820/1‑138 (MQ=255)
                                                                                                                                 aaaTAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTAc                     <  2:901817/150‑1 (MQ=255)
                                                                                                                                    ttcaCGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGt                  >  2:1455736/4‑150 (MQ=255)
                                                                                                                                    tAAACGGCCCATCCATGAGGAATGGGCAGTGAAAGGAGATTACGCGACCCGGAGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAAAAACGTCAGTCACTTTGTAAACCGTTTCACATTTAATTTGTAAAGt                  >  2:614630/1‑150 (MQ=255)
                                                                                                                                     tcaCGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTg                 >  2:1280590/3‑150 (MQ=255)
                                                                                                                                      caCGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGc                >  2:933947/2‑150 (MQ=255)
                                                                                                                                        cGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCg              >  1:1085870/1‑150 (MQ=255)
                                                                                                                                         ggCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGt             >  2:555090/1‑150 (MQ=255)
                                                                                                                                         ggCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGt             >  2:304217/1‑150 (MQ=255)
                                                                                                                                         ggCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGt             <  2:1616392/150‑1 (MQ=255)
                                                                                                                                         ggCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGt             <  2:821651/150‑1 (MQ=255)
                                                                                                                                               tccatGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCt       >  1:213717/1‑150 (MQ=255)
                                                                                                                                               tccatGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCt       <  1:1532333/150‑1 (MQ=255)
                                                                                                                                                 catGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTgg     <  2:1356624/150‑1 (MQ=255)
                                                                                                                                                  atGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGc    <  1:801068/150‑1 (MQ=255)
                                                                                                                                                  atGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGc    <  2:746941/150‑1 (MQ=255)
                                                                                                                                                  atGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGc    >  1:615410/1‑150 (MQ=255)
                                                                                                                                                   tGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCt   <  2:101338/150‑1 (MQ=255)
                                                                                                                                                    gAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTg  >  1:505382/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTG  >  NC_000913/1346807‑1347104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑