Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,346,957 G→T 11.3% intergenic (+57/+21) ymiB → / ← rnb putative protein YmiB/RNase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,346,9570GT11.3% 305.9 / 23.7 106intergenic (+57/+21)ymiB/rnbputative protein YmiB/RNase II
Reads supporting (aligned to +/- strand):  ref base G (42/52);  new base T (8/4);  total (50/56)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.48e-01

Only 100 of 109 total aligned reads displayed.

AAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTGCA  >  NC_000913/1346808‑1347106
                                                                                                                                                     |                                                                                                                                                     
aaaTTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAg                                                                                                                                                       <  2:1088135/150‑1 (MQ=255)
  aTTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGa                                                                                                                                                     >  1:1567249/1‑150 (MQ=255)
      aCGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATgg                                                                                                                                                 <  1:1033939/150‑1 (MQ=255)
       cggcggCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATggg                                                                                                                                                >  1:718769/1‑150 (MQ=255)
        ggcggcTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGc                                                                                                                                               >  1:531216/1‑150 (MQ=255)
        ggcggcTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGc                                                                                                                                               >  1:29109/1‑150 (MQ=255)
            gcTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATTCCTCATGGATGGGCCGtt                                                                                                                                           <  1:246633/150‑2 (MQ=255)
              tAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGaa                                                                                                                                         >  2:164221/1‑150 (MQ=255)
              aaaaCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTTGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATTCCTCATGGATGGGCCGttta                                                                                                                                         <  2:593986/149‑4 (MQ=255)
               aaaCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGTAAAACGCCAGTTTTCAAACGAAAGTCATTTAAAAAATGTTCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGaaa                                                                                                                                        <  2:559443/150‑1 (MQ=255)
               aaaCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATTCCTCATGGATGGGCCGtttat                                                                                                                                        <  2:1479961/150‑5 (MQ=255)
                aaCGCATTGTTTTTTTTATATATTTATTTTTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAg                                                                                                                                       <  1:1091288/150‑1 (MQ=255)
                aaCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATTCCTCATGGATGGGCCGtttatt                                                                                                                                       <  1:784871/150‑6 (MQ=255)
                aaCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGACTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCAATCCATGAGGAATGGGCCTTCAAAg                                                                                                                                       >  2:801068/1‑150 (MQ=255)
                   gCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGgag                                                                                                                                    <  2:789616/150‑1 (MQ=255)
                       tGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTa                                                                                                                                >  2:597585/1‑150 (MQ=255)
                        gTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGAttgc                                                                                                                               >  1:702017/1‑148 (MQ=255)
                        gTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTAc                                                                                                                               >  2:1532333/1‑150 (MQ=255)
                          tttttttATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTAcgc                                                                                                                             <  2:173562/150‑1 (MQ=255)
                               ttATATATTTATTTGTAATCCAGTTTTGGAAAACCGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCGTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCg                                                                                                                        >  1:516772/1‑150 (MQ=255)
                                tatataTTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCgg                                                                                                                       >  1:1306185/1‑150 (MQ=255)
                                  tatatTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGgc                                                                                                                     >  1:33591/1‑150 (MQ=255)
                                    tatttattTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGgcgc                                                                                                                   >  2:1287557/1‑150 (MQ=255)
                                    tatttattTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGgcgc                                                                                                                   >  1:1480982/1‑150 (MQ=255)
                                    tatttattTGTAATCCAGTTTTGGAAAAACGCCAGTTTACAAACGAAAGTCAGTTAAAAAATCTGCCAGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAACGAGATTACTCGCCCGGgcgc                                                                                                                   >  2:1535922/1‑150 (MQ=255)
                                     atttatttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGgcgcg                                                                                                                  <  2:1124548/150‑1 (MQ=255)
                                      tttatttTTAATACAGTATTGCAAAAAAGCAAGATTACAAACGAAAGTGAGTTAAAAAATCAGAATGGAAATAACGAAGTTAGATGGGGGAAATAAACGGCCAATCCATGAGGAATGGGACGTGAAATGAGATTACGCGACCGGgcgcgc                                                                                                                 >  2:724015/1‑150 (MQ=255)
                                       ttatttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCTAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCa                                                                                                                <  1:1529838/150‑1 (MQ=255)
                                        tatttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAATTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGgaga                                                                                                                                   >  2:468425/1‑130 (MQ=255)
                                        tatttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAATTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGgaga                                                                                                                                   <  1:468425/130‑1 (MQ=255)
                                         atttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCaat                                                                                                              <  2:1086715/150‑1 (MQ=255)
                                            tGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCaataat                                                                                                           >  1:234277/1‑150 (MQ=255)
                                            tGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCaataat                                                                                                           >  2:210067/1‑150 (MQ=255)
                                                 tCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTAAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAAtgctgc                                                                                                      >  2:729174/1‑150 (MQ=255)
                                                  ccAGTTTTGGAAAAACGCCAGATTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCAATCCATGAGGAATGGGCCGAGAAAGTAGATTACGCGACCGGGCGCGCAATAATGCTGCg                                                                                                     >  2:315702/1‑150 (MQ=255)
                                                     gTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGt                                                                                                  <  1:1450690/150‑1 (MQ=255)
                                                        ttGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTc                                                                                               <  1:1271381/150‑1 (MQ=255)
                                                        ttGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTc                                                                                               >  1:1272723/1‑150 (MQ=255)
                                                        ttGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGCGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTc                                                                                               >  2:1148516/1‑150 (MQ=255)
                                                          ggAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCa                                                                                             <  1:232665/150‑1 (MQ=255)
                                                          ggAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCa                                                                                             <  2:509109/150‑1 (MQ=255)
                                                             aaaaCGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGc                                                                                          <  1:307842/150‑1 (MQ=255)
                                                              aaaCGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCg                                                                                         <  2:703330/150‑1 (MQ=255)
                                                               aaCGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCgg                                                                                        >  1:589712/1‑150 (MQ=255)
                                                                 cGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGAc                                                                                      >  2:470388/1‑150 (MQ=255)
                                                                       ttttCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGCAGGTTTCGCGGGGAAATAAACGGCCCATCCATGCGGAATGGGCCGTGAAATGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGc                                                                                <  1:23436/150‑1 (MQ=255)
                                                                       ttttCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGc                                                                                <  2:531216/150‑1 (MQ=255)
                                                                          tCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCCGCGGGTTTCCATGCGGACTTCGGCAAt                                                                             <  1:148080/150‑1 (MQ=255)
                                                                           cAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATa                                                                            >  1:1356624/1‑150 (MQ=255)
                                                                           cAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATa                                                                            <  1:470388/150‑1 (MQ=255)
                                                                            aaaCGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAg                                                                           >  2:1019550/1‑150 (MQ=255)
                                                                            aaaCGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAg                                                                           <  2:226789/150‑1 (MQ=255)
                                                                               cGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGCGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGa                                                                        <  1:1148516/150‑1 (MQ=255)
                                                                                gAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGAc                                                                       >  2:1251709/1‑150 (MQ=255)
                                                                                   aGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTc                                                                    <  2:25438/150‑1 (MQ=255)
                                                                                      cAGTTAATAAATCTGCCTGGTTATAACGAAGGTAGTGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCGGTGTATGGAGATTACGCGAGCGGGCGCGCAATATTGCTGCGGGTTTCCTTGCGGACTTCGGCAATAGTGACGTCAAt                                                                 <  1:1166141/150‑1 (MQ=255)
                                                                                      cAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAAt                                                                 >  1:366897/1‑150 (MQ=255)
                                                                                        gTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATaa                                                               >  2:819853/1‑150 (MQ=255)
                                                                                              aaaTCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGATTTCGGCAATAGTGACGTCAATAACgtcag                                                         <  1:804492/150‑1 (MQ=255)
                                                                                                 tCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACgtcagtca                                                      <  1:233797/150‑1 (MQ=255)
                                                                                                  cTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTTATTGACgtcactatt                                                     <  1:589727/150‑6 (MQ=255)
                                                                                                   tGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTTATTGACgtcactattg                                                    <  2:1508108/150‑7 (MQ=255)
                                                                                                      cTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGt                                                                          >  1:1151433/1‑125 (MQ=255)
                                                                                                      cTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGt                                                                          <  2:1151433/125‑1 (MQ=255)
                                                                                                         gATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTaa                                              >  1:1616392/1‑150 (MQ=255)
                                                                                                           tataACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAAc                                            >  1:821651/1‑150 (MQ=255)
                                                                                                            ataACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAAcc                                           <  1:144663/150‑1 (MQ=255)
                                                                                                                cGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGttt                                       >  2:1455172/1‑150 (MQ=255)
                                                                                                                 gAAGTTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTc                                      <  2:1445784/150‑1 (MQ=255)
                                                                                                                  aaGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACGGTTTCa                                     <  2:354871/150‑1 (MQ=255)
                                                                                                                  aaGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCa                                     <  1:1522506/150‑1 (MQ=255)
                                                                                                                     gTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCt                                  <  1:210067/150‑1 (MQ=255)
                                                                                                                         agCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTaa                              <  1:1245387/150‑1 (MQ=255)
                                                                                                                            ggggAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAAttt                           <  2:718769/150‑1 (MQ=255)
                                                                                                                             gggAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTg                          <  2:121947/150‑1 (MQ=255)
                                                                                                                             gggAAATAAACGGCCCATCCATGAGGAATGGGCCATGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATTCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTTTAAACCGTTTCACCTTTAAtttt                          >  2:462478/1‑149 (MQ=255)
                                                                                                                               gAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTa                        <  2:1593523/150‑1 (MQ=255)
                                                                                                                                cctttcaCGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTAc                       >  1:1185619/7‑150 (MQ=255)
                                                                                                                                aaaTAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCAcc                                   <  2:1561820/138‑1 (MQ=255)
                                                                                                                                aaaTAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCAcc                                   >  1:1561820/1‑138 (MQ=255)
                                                                                                                                aaaTAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTAc                       <  2:901817/150‑1 (MQ=255)
                                                                                                                                  tttcaCGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAg                     >  1:1618143/5‑150 (MQ=255)
                                                                                                                                   ttcaCGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGt                    >  2:1455736/4‑150 (MQ=255)
                                                                                                                                   tAAACGGCCCATCCATGAGGAATGGGCAGTGAAAGGAGATTACGCGACCCGGAGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAAAAACGTCAGTCACTTTGTAAACCGTTTCACATTTAATTTGTAAAGt                    >  2:614630/1‑150 (MQ=255)
                                                                                                                                    tcaCGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTg                   >  2:1280590/3‑150 (MQ=255)
                                                                                                                                     caCGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGc                  >  2:933947/2‑150 (MQ=255)
                                                                                                                                       cGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCg                >  1:1085870/1‑150 (MQ=255)
                                                                                                                                        ggCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGt               >  2:555090/1‑150 (MQ=255)
                                                                                                                                        ggCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGt               >  2:304217/1‑150 (MQ=255)
                                                                                                                                        ggCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGt               <  2:1616392/150‑1 (MQ=255)
                                                                                                                                        ggCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGt               <  2:821651/150‑1 (MQ=255)
                                                                                                                                              tccatGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCt         >  1:213717/1‑150 (MQ=255)
                                                                                                                                              tccatGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCt         <  1:1532333/150‑1 (MQ=255)
                                                                                                                                                catGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTgg       <  2:1356624/150‑1 (MQ=255)
                                                                                                                                                 atGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGc      <  2:746941/150‑1 (MQ=255)
                                                                                                                                                 atGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGc      >  1:615410/1‑150 (MQ=255)
                                                                                                                                                  tGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCt     <  2:101338/150‑1 (MQ=255)
                                                                                                                                                   gAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTg    >  1:505382/1‑150 (MQ=255)
                                                                                                                                                    aGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCTTTTTCCTGGATGc   <  2:29109/150‑1 (MQ=255)
                                                                                                                                                     ggAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTGCa  >  2:437444/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTGCA  >  NC_000913/1346808‑1347106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑