Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,346,959 A→G 11.2% intergenic (+59/+19) ymiB → / ← rnb putative protein YmiB/RNase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,346,9590AG11.2% 280.7 / 23.1 107intergenic (+59/+19)ymiB/rnbputative protein YmiB/RNase II
Reads supporting (aligned to +/- strand):  ref base A (42/53);  new base G (8/4);  total (50/57)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01

Only 100 of 109 total aligned reads displayed.

ATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTGCAAA  >  NC_000913/1346810‑1347108
                                                                                                                                                     |                                                                                                                                                     
aTTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGa                                                                                                                                                       >  1:1567249/1‑150 (MQ=255)
    aCGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATgg                                                                                                                                                   <  1:1033939/150‑1 (MQ=255)
     cggcggCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATggg                                                                                                                                                  >  1:718769/1‑150 (MQ=255)
      ggcggcTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGc                                                                                                                                                 >  1:531216/1‑150 (MQ=255)
      ggcggcTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGc                                                                                                                                                 >  1:29109/1‑150 (MQ=255)
          gcTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATTCCTCATGGATGGGCCGtt                                                                                                                                             <  1:246633/150‑2 (MQ=255)
            tAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGaa                                                                                                                                           >  2:164221/1‑150 (MQ=255)
            aaaaCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTTGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATTCCTCATGGATGGGCCGttta                                                                                                                                           <  2:593986/149‑4 (MQ=255)
             aaaCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGTAAAACGCCAGTTTTCAAACGAAAGTCATTTAAAAAATGTTCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGaaa                                                                                                                                          <  2:559443/150‑1 (MQ=255)
             aaaCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATTCCTCATGGATGGGCCGtttat                                                                                                                                          <  2:1479961/150‑5 (MQ=255)
              aaCGCATTGTTTTTTTTATATATTTATTTTTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAg                                                                                                                                         <  1:1091288/150‑1 (MQ=255)
              aaCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGACTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCAATCCATGAGGAATGGGCCTTCAAAg                                                                                                                                         >  2:801068/1‑150 (MQ=255)
                 gCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGgag                                                                                                                                      <  2:789616/150‑1 (MQ=255)
                 gCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGgag                                                                                                                                      <  1:449751/150‑1 (MQ=255)
                     tGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTa                                                                                                                                  >  2:597585/1‑150 (MQ=255)
                      gTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGAttgc                                                                                                                                 >  1:702017/1‑148 (MQ=255)
                      gTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTAc                                                                                                                                 >  2:1532333/1‑150 (MQ=255)
                        tttttttATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTAcgc                                                                                                                               <  2:173562/150‑1 (MQ=255)
                             ttATATATTTATTTGTAATCCAGTTTTGGAAAACCGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCGTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCg                                                                                                                          >  1:516772/1‑150 (MQ=255)
                              tatataTTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCgg                                                                                                                         >  1:1306185/1‑150 (MQ=255)
                                tatatTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGgc                                                                                                                       >  1:33591/1‑150 (MQ=255)
                                  tatttattTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGgcgc                                                                                                                     >  1:1480982/1‑150 (MQ=255)
                                  tatttattTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGgcgc                                                                                                                     >  2:1287557/1‑150 (MQ=255)
                                  tatttattTGTAATCCAGTTTTGGAAAAACGCCAGTTTACAAACGAAAGTCAGTTAAAAAATCTGCCAGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAACGAGATTACTCGCCCGGgcgc                                                                                                                     >  2:1535922/1‑150 (MQ=255)
                                   atttatttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGgcgcg                                                                                                                    <  2:1124548/150‑1 (MQ=255)
                                    tttatttTTAATACAGTATTGCAAAAAAGCAAGATTACAAACGAAAGTGAGTTAAAAAATCAGAATGGAAATAACGAAGTTAGATGGGGGAAATAAACGGCCAATCCATGAGGAATGGGACGTGAAATGAGATTACGCGACCGGgcgcgc                                                                                                                   >  2:724015/1‑150 (MQ=255)
                                     ttatttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCTAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCa                                                                                                                  <  1:1529838/150‑1 (MQ=255)
                                      tatttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAATTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGgaga                                                                                                                                     >  2:468425/1‑130 (MQ=255)
                                      tatttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAATTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGgaga                                                                                                                                     <  1:468425/130‑1 (MQ=255)
                                       atttGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCaat                                                                                                                <  2:1086715/150‑1 (MQ=255)
                                          tGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCaataat                                                                                                             >  1:234277/1‑150 (MQ=255)
                                          tGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCaataat                                                                                                             >  2:210067/1‑150 (MQ=255)
                                               tCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTAAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAAtgctgc                                                                                                        >  2:729174/1‑150 (MQ=255)
                                                ccAGTTTTGGAAAAACGCCAGATTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCAATCCATGAGGAATGGGCCGAGAAAGTAGATTACGCGACCGGGCGCGCAATAATGCTGCg                                                                                                       >  2:315702/1‑150 (MQ=255)
                                                   gTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGt                                                                                                    <  1:1450690/150‑1 (MQ=255)
                                                      ttGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTc                                                                                                 >  1:1272723/1‑150 (MQ=255)
                                                      ttGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTc                                                                                                 <  1:1271381/150‑1 (MQ=255)
                                                      ttGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGCGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTc                                                                                                 >  2:1148516/1‑150 (MQ=255)
                                                        ggAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCa                                                                                               <  1:232665/150‑1 (MQ=255)
                                                        ggAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCa                                                                                               <  2:509109/150‑1 (MQ=255)
                                                           aaaaCGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGc                                                                                            <  1:307842/150‑1 (MQ=255)
                                                            aaaCGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCg                                                                                           <  2:703330/150‑1 (MQ=255)
                                                             aaCGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCgg                                                                                          >  1:589712/1‑150 (MQ=255)
                                                               cGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGAc                                                                                        >  2:470388/1‑150 (MQ=255)
                                                                     ttttCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGCAGGTTTCGCGGGGAAATAAACGGCCCATCCATGCGGAATGGGCCGTGAAATGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGc                                                                                  <  1:23436/150‑1 (MQ=255)
                                                                     ttttCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGc                                                                                  <  2:531216/150‑1 (MQ=255)
                                                                        tCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCCGCGGGTTTCCATGCGGACTTCGGCAAt                                                                               <  1:148080/150‑1 (MQ=255)
                                                                         cAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATa                                                                              <  1:470388/150‑1 (MQ=255)
                                                                         cAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATa                                                                              >  1:1356624/1‑150 (MQ=255)
                                                                          aaaCGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAg                                                                             <  2:226789/150‑1 (MQ=255)
                                                                          aaaCGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAg                                                                             >  2:1019550/1‑150 (MQ=255)
                                                                             cGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGCGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGa                                                                          <  1:1148516/150‑1 (MQ=255)
                                                                              gAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGAc                                                                         >  2:1251709/1‑150 (MQ=255)
                                                                                 aGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTc                                                                      <  2:25438/150‑1 (MQ=255)
                                                                                    cAGTTAATAAATCTGCCTGGTTATAACGAAGGTAGTGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCGGTGTATGGAGATTACGCGAGCGGGCGCGCAATATTGCTGCGGGTTTCCTTGCGGACTTCGGCAATAGTGACGTCAAt                                                                   <  1:1166141/150‑1 (MQ=255)
                                                                                      gTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATaa                                                                 >  2:819853/1‑150 (MQ=255)
                                                                                        tAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACg                                                               >  1:1421909/1‑150 (MQ=255)
                                                                                            aaaTCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGATTTCGGCAATAGTGACGTCAATAACgtcag                                                           <  1:804492/150‑1 (MQ=255)
                                                                                               tCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACgtcagtca                                                        <  1:233797/150‑1 (MQ=255)
                                                                                                cTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTTATTGACgtcactatt                                                       <  1:589727/150‑6 (MQ=255)
                                                                                                 tGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTTATTGACgtcactattg                                                      <  2:1508108/150‑7 (MQ=255)
                                                                                                  gCCTGCATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACtt                                                     <  1:183570/150‑1 (MQ=255)
                                                                                                    cTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGt                                                                            >  1:1151433/1‑125 (MQ=255)
                                                                                                    cTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGt                                                                            <  2:1151433/125‑1 (MQ=255)
                                                                                                       gATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTaa                                                >  1:1616392/1‑150 (MQ=255)
                                                                                                         tataACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAAc                                              >  1:821651/1‑150 (MQ=255)
                                                                                                          ataACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAAcc                                             <  1:144663/150‑1 (MQ=255)
                                                                                                              cGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGttt                                         >  2:1455172/1‑150 (MQ=255)
                                                                                                               gAAGTTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTc                                        <  2:1445784/150‑1 (MQ=255)
                                                                                                               gAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTc                                        <  2:1567249/150‑1 (MQ=255)
                                                                                                                aaGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACGGTTTCa                                       <  2:354871/150‑1 (MQ=255)
                                                                                                                aaGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCa                                       <  1:1522506/150‑1 (MQ=255)
                                                                                                                   gTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCt                                    <  1:210067/150‑1 (MQ=255)
                                                                                                                       agCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTaa                                <  1:1245387/150‑1 (MQ=255)
                                                                                                                          ggggAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAAttt                             <  2:718769/150‑1 (MQ=255)
                                                                                                                           gggAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTg                            <  2:121947/150‑1 (MQ=255)
                                                                                                                           gggAAATAAACGGCCCATCCATGAGGAATGGGCCATGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATTCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTTTAAACCGTTTCACCTTTAAtttt                            >  2:462478/1‑149 (MQ=255)
                                                                                                                              cctttcaCGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTAc                         >  1:1185619/7‑150 (MQ=255)
                                                                                                                              aaaTAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCAcc                                     <  2:1561820/138‑1 (MQ=255)
                                                                                                                              aaaTAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCAcc                                     >  1:1561820/1‑138 (MQ=255)
                                                                                                                              aaaTAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTAc                         <  2:901817/150‑1 (MQ=255)
                                                                                                                                 ttcaCGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGt                      >  2:1455736/4‑150 (MQ=255)
                                                                                                                                 tAAACGGCCCATCCATGAGGAATGGGCAGTGAAAGGAGATTACGCGACCCGGAGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAAAAACGTCAGTCACTTTGTAAACCGTTTCACATTTAATTTGTAAAGt                      >  2:614630/1‑150 (MQ=255)
                                                                                                                                   caCGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGc                    >  2:933947/2‑150 (MQ=255)
                                                                                                                                     cGGCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCg                  >  1:1085870/1‑150 (MQ=255)
                                                                                                                                      ggCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGt                 >  2:555090/1‑150 (MQ=255)
                                                                                                                                      ggCCCATTCCTCATGGATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGt                 >  2:304217/1‑150 (MQ=255)
                                                                                                                                      ggCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGt                 <  2:1616392/150‑1 (MQ=255)
                                                                                                                                      ggCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGt                 <  2:821651/150‑1 (MQ=255)
                                                                                                                                            tccatGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCt           >  1:213717/1‑150 (MQ=255)
                                                                                                                                            tccatGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCt           <  1:1532333/150‑1 (MQ=255)
                                                                                                                                              catGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTgg         <  2:1356624/150‑1 (MQ=255)
                                                                                                                                               atGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGc        <  1:801068/150‑1 (MQ=255)
                                                                                                                                               atGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGc        <  2:746941/150‑1 (MQ=255)
                                                                                                                                               atGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGc        >  1:615410/1‑150 (MQ=255)
                                                                                                                                                tGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCt       <  2:101338/150‑1 (MQ=255)
                                                                                                                                                 gAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTg      >  1:505382/1‑150 (MQ=255)
                                                                                                                                                  aGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCTTTTTCCTGGATGc     <  2:29109/150‑1 (MQ=255)
                                                                                                                                                   ggAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTGCa    >  2:437444/1‑150 (MQ=255)
                                                                                                                                                     aaTGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTGCaaa  <  2:362715/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTCAGTCACTTTGTAAACCGTTTCACCTTTAATTTGTACAGTGCCGTTTTCCTGGCTGCAAA  >  NC_000913/1346810‑1347108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑