Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,406,093 A→G 5.7% I39V (ATC→GTC)  smrA → DNA endonuclease SmrA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,406,0930AG 5.7% 267.4 / 4.9 87I39V (ATC→GTC) smrADNA endonuclease SmrA
Reads supporting (aligned to +/- strand):  ref base A (48/34);  new base G (3/2);  total (51/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

ATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAATATCCGCAACAGGCGAGCCTG  >  NC_000913/1405944‑1406242
                                                                                                                                                     |                                                                                                                                                     
aTCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTa                                                                                                                                                       >  1:29248/1‑150 (MQ=255)
  cATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATc                                                                                                                                                     >  1:595769/1‑150 (MQ=255)
   atatCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCg                                                                                                                                                    >  2:1463430/1‑150 (MQ=255)
     atCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGac                                                                                                                                                  >  2:870218/1‑150 (MQ=255)
     atCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGac                                                                                                                                                  >  2:125881/1‑150 (MQ=255)
           ctctTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTg                                                                                                                                            >  1:1007363/1‑150 (MQ=255)
            tctTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGc                                                                                                                                           >  2:740994/1‑150 (MQ=255)
            tctTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGc                                                                                                                                           <  1:1342511/150‑1 (MQ=255)
             ctTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCa                                                                                                                                          >  2:337704/1‑150 (MQ=255)
              ttAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTGTCGATACGCTGcgg                                                                                                                                         <  1:687408/150‑3 (MQ=255)
               tAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTGTCGATACGCTGCGGc                                                                                                                                        <  1:1542552/150‑1 (MQ=255)
                 aTTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCtt                                                                                                                                      >  1:110172/1‑150 (MQ=255)
                  ttGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTg                                                                                                                                     <  2:743584/150‑1 (MQ=255)
                  ttGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTg                                                                                                                                     <  2:767602/150‑1 (MQ=255)
                  ttGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTg                                                                                                                                     <  1:1486062/150‑1 (MQ=255)
                       ggaggaTGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAAt                                                                                                                                >  1:646201/1‑150 (MQ=255)
                              gTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCAGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCACAGTGCGCCGAAGCGTATCGACACGCTGCAGCTTGATAATTTCCTca                                                                                                                         >  1:1605235/1‑150 (MQ=255)
                                aaCATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTcacc                                                                                                                       <  1:1033643/150‑1 (MQ=255)
                                aaCATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTcacc                                                                                                                       >  2:776197/1‑150 (MQ=255)
                                    tGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCg                                                                                                                   >  2:364457/1‑150 (MQ=255)
                                          ttGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTtc                                                                                                             >  2:5875/1‑150 (MQ=255)
                                               gacAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGAc                                                                                                        <  2:29248/150‑1 (MQ=255)
                                               gacAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGAc                                                                                                        <  2:239261/150‑1 (MQ=255)
                                               gacAAATCGCTGTTTCTTGACGCCAAGGAAGATGACCAGCCGCTGAAACGTGCTACCGATGTCAACTGGCATACAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCATGATAATTACCTAACCACCGGATTTCTCGAc                                                                                                        >  2:1072522/1‑150 (MQ=255)
                                                acAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGAca                                                                                                       >  1:1069463/1‑150 (MQ=255)
                                                      cGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATcc                                                                                                 >  1:216891/1‑150 (MQ=255)
                                                        cTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATcccc                                                                                               >  2:408584/1‑149 (MQ=255)
                                                         tGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCAc                                                                                              <  1:48217/150‑1 (MQ=255)
                                                            ttCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTaa                                                                                           >  1:685373/1‑150 (MQ=255)
                                                                tGACGCCATTGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCAAAAGCCTAACCAACTTGCGAAGCAGCGTATCGACACGCTCCAGCTTGATAATTTCCTCAACAACGGATTTCTAGACATCATCCCACTAAGTCa                                                                                       >  2:676642/1‑150 (MQ=255)
                                                                tGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCa                                                                                       >  1:1606478/1‑150 (MQ=255)
                                                                   cGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGcc                                                                                    >  1:741562/1‑150 (MQ=255)
                                                                   cGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGcc                                                                                    >  1:1380530/1‑150 (MQ=255)
                                                                   cGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGcc                                                                                    <  2:486741/150‑1 (MQ=255)
                                                                     ccATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCg                                                                                   >  1:1488670/1‑149 (MQ=255)
                                                                     ccATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCg                                                                                   <  2:1488670/149‑1 (MQ=255)
                                                                      cATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGTTGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCt                                                                                 >  1:80728/1‑150 (MQ=255)
                                                                       aTGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTg                                                                                <  1:776197/150‑1 (MQ=255)
                                                                       aTGGAAGATGTCCAGACGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTg                                                                                <  1:803417/150‑1 (MQ=255)
                                                                        tGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTgg                                                                               >  2:336480/1‑150 (MQ=255)
                                                                         ggAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGa                                                                              >  1:159180/1‑150 (MQ=255)
                                                                          gAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAg                                                                             <  1:43958/150‑1 (MQ=255)
                                                                           aaGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCCCTAAGTCAGCCGCTGGAGt                                                                            >  1:403840/1‑150 (MQ=255)
                                                                                gTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTcgg                                                                       >  1:1758/1‑150 (MQ=255)
                                                                                 tCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTcggc                                                                      >  1:712251/1‑150 (MQ=255)
                                                                                  ccAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTcggcg                                                                     <  1:1329457/150‑1 (MQ=255)
                                                                                   cAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTcggcgg                                                                    <  1:52188/150‑1 (MQ=255)
                                                                                   cAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTcggcgg                                                                    <  2:1209495/150‑1 (MQ=255)
                                                                                    aGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCggg                                                                   >  2:638252/1‑150 (MQ=255)
                                                                                     gCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGa                                                                  >  2:801074/1‑150 (MQ=255)
                                                                                        gCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGAGATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAgg                                                               <  1:300055/150‑1 (MQ=255)
                                                                                           gAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGtt                                                            <  1:1588868/150‑1 (MQ=255)
                                                                                             aaCGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGc                                                          <  1:1011081/150‑1 (MQ=255)
                                                                                               cGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCaa                                                        >  2:1100005/1‑150 (MQ=255)
                                                                                                      ccGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATgggg                                                 >  1:1416505/1‑150 (MQ=255)
                                                                                                      ccGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATgggg                                                 <  1:125881/150‑1 (MQ=255)
                                                                                                       cGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGt                                                <  2:1007363/150‑1 (MQ=255)
                                                                                                           gTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTg                                            <  1:1463430/150‑1 (MQ=255)
                                                                                                           gTCCACTGGCATCCAACGCGTAACCAACGGGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTg                                            <  1:1100005/150‑1 (MQ=255)
                                                                                                            tCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTgg                                           >  1:201921/1‑150 (MQ=255)
                                                                                                              cACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTTGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGAt                                         <  1:676642/150‑1 (MQ=255)
                                                                                                                  ggCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGc                                     >  1:320861/1‑150 (MQ=255)
                                                                                                                   gCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCt                                    <  2:646201/150‑1 (MQ=255)
                                                                                                                   gCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCt                                    >  1:202957/1‑150 (MQ=255)
                                                                                                                    cATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTg                                   >  2:1070661/1‑150 (MQ=255)
                                                                                                                     aTCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTgc                                  <  1:801074/150‑1 (MQ=255)
                                                                                                                      tCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTgcg                                 <  2:110172/150‑1 (MQ=255)
                                                                                                                       ccAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTgcgc                                <  1:740994/150‑1 (MQ=255)
                                                                                                                         aaCGCGAAACCAAAGTGCGCCGCAGCGAAACGACACGCTGCCGCTTGATAATTTCCTCACCACCGCATTTCTCGACATCATCCCACTAATACAGCAGCTCGACTTTAGGCCGGAACTGATGAAACATTTGCTGCAGCATAAGCTGCGCat                              >  2:557867/1‑149 (MQ=255)
                                                                                                                             cgTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGt                          <  2:1380530/150‑1 (MQ=255)
                                                                                                                             cgTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGt                          >  1:1247580/1‑150 (MQ=255)
                                                                                                                                 aCCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAat                      >  1:791693/1‑150 (MQ=255)
                                                                                                                                  ccAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGTAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAata                     >  2:324323/1‑150 (MQ=255)
                                                                                                                                  ccAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAata                     >  1:1006441/1‑150 (MQ=255)
                                                                                                                                  ccAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAata                     <  2:216891/150‑1 (MQ=255)
                                                                                                                                   cAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTCGAGTTTCGTCGCGAAGGGTTGCAACATCTGGTGCTGGATAAGCTGCGCAGTGGTAAataa                    >  2:185619/1‑149 (MQ=255)
                                                                                                                                    aaCGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGT‑GTAAATATcc                  >  1:950861/1‑150 (MQ=255)
                                                                                                                                     aCGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAATATcc                  <  2:1069463/150‑1 (MQ=255)
                                                                                                                                        tGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAATATCCGCa               <  1:364457/150‑1 (MQ=255)
                                                                                                                                         gcgcCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTCCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAATATCCGCaa              >  2:633949/1‑150 (MQ=255)
                                                                                                                                         aagctgcAGCGTGTCGATACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAATATCCGCaa              >  1:185597/6‑150 (MQ=255)
                                                                                                                                           gctgcAGCGTGTCGATACGCTGCAGCTTGATAATTTCCTCCCCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAATATCCGCAACa            >  1:1109720/4‑150 (MQ=255)
                                                                                                                                           gccgcAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCAGCGGATTTCTCGACATCATGCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCGGGATAAGCTGCGCAGTGGTAAATATCCGCAAcg            >  2:1590073/1‑149 (MQ=255)
                                                                                                                                           gccgcAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAATATCCGCAACa            >  1:559399/1‑150 (MQ=255)
                                                                                                                                            ctgcAGCGTGTCGATACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCGGTGGTAAATATCCGCAACAg           >  1:721946/3‑150 (MQ=255)
                                                                                                                                                 gCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAATATCCGCAACAGGCGAg      <  2:1606478/150‑1 (MQ=255)
                                                                                                                                                  cGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAATATCCGCAACAGGCGAGc     <  2:438150/150‑1 (MQ=255)
                                                                                                                                                    tATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAATATCCGCAACAGGCGAGCCt   >  1:81715/1‑150 (MQ=255)
                                                                                                                                                     aTCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAATATCCGCAACAGGCGAGCCTg  >  2:1099420/1‑150 (MQ=255)
                                                                                                                                                     aTCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAATATCCGCAACAGGCGAGCCTg  >  1:359672/1‑150 (MQ=255)
                                                                                                                                                     aTCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAATATCCGCAACAGGCGAGCCTg  >  1:1601389/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCGTATCGACACGCTGCAGCTTGATAATTTCCTCACCACCGGATTTCTCGACATCATCCCACTAAGTCAGCCGCTGGAGTTTCGGCGGGAAGGGTTGCAACATGGGGTGCTGGATAAGCTGCGCAGTGGTAAATATCCGCAACAGGCGAGCCTG  >  NC_000913/1405944‑1406242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑