Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,408,287 A→G 8.7% S80G (AGC→GGC)  zntB → Zn(2(+)):H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,408,2870AG 8.7% 218.5 / 10.5 80S80G (AGC→GGC) zntBZn(2(+)):H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (32/41);  new base G (4/3);  total (36/44)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01

AAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGA  >  NC_000913/1408139‑1408435
                                                                                                                                                    |                                                                                                                                                    
aaaTACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAg                                                                                                                                                     <  2:847030/150‑1 (MQ=255)
   tACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCg                                                                                                                                                  >  1:1519742/1‑150 (MQ=255)
      aGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGtct                                                                                                                                               <  1:563556/150‑1 (MQ=255)
        aTGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATACCGTACGTGATGCGCTGGCGGGCGAGAGCCCCCGTCCCCGAGTCAGCCGTCTCg                                                                                                                                             >  2:657404/1‑150 (MQ=255)
         tgtgATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCgg                                                                                                                                            >  2:994427/1‑150 (MQ=255)
         tgtgATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCgg                                                                                                                                            <  2:233675/150‑1 (MQ=255)
           tgattgatGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTg                                                                                                                                          <  2:520343/150‑1 (MQ=255)
           tgattgatGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTg                                                                                                                                          <  2:525811/150‑1 (MQ=255)
            gattgatGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGa                                                                                                                                         >  2:790597/1‑150 (MQ=255)
            gattgatGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGACGGCTGACTCGGGGa                                                                                                                                         <  2:1345081/150‑1 (MQ=255)
               tgatgaAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCTGTGAAgg                                                                                                                                      <  1:1392344/150‑1 (MQ=255)
               tgatgaAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAgg                                                                                                                                      <  2:463565/150‑1 (MQ=255)
                 atgaAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCa                                                                                                                                    >  1:1559975/1‑150 (MQ=255)
                  tgaAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCAc                                                                                                                                   >  2:535703/1‑150 (MQ=255)
                     aGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCt                                                                                                                                >  2:1009335/1‑150 (MQ=255)
                     aGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCt                                                                                                                                <  2:1273472/150‑1 (MQ=255)
                     aGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGAGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCt                                                                                                                                <  2:954651/150‑1 (MQ=255)
                      gcgcATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTg                                                                                                                               <  2:312305/150‑1 (MQ=255)
                      gcgcATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTg                                                                                                                               <  2:1466559/150‑1 (MQ=255)
                       cgcATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGa                                                                                                                              >  1:289616/1‑150 (MQ=255)
                        gcATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGAt                                                                                                                             >  2:1006366/1‑150 (MQ=255)
                        gcATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGAt                                                                                                                             >  1:733343/1‑150 (MQ=255)
                        gcATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGAt                                                                                                                             <  1:555001/150‑1 (MQ=255)
                          aTCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTa                                                                                                                           <  1:1383668/150‑1 (MQ=255)
                            cccTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACa                                                                                                                         >  2:1390001/1‑150 (MQ=255)
                                    gCTCCACCTTAATTATGTACACCATGTTAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTg                                                                                                                 <  2:234303/150‑1 (MQ=255)
                                    gCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTg                                                                                                                 <  1:1009335/150‑1 (MQ=255)
                                        cACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGtata                                                                                                             <  2:443297/150‑1 (MQ=255)
                                         aCCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATaa                                                                                                            <  2:1521433/150‑1 (MQ=255)
                                           cTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAAc                                                                                                          >  1:1002071/1‑150 (MQ=255)
                                              aaTTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGgc                                                                                                       <  1:832676/150‑1 (MQ=255)
                                              aaTTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGgc                                                                                                       <  1:1509827/150‑1 (MQ=255)
                                               aTTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGgca                                                                                                      <  1:695413/150‑1 (MQ=255)
                                                 tATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGgcagc                                                                                                    >  2:405244/1‑150 (MQ=255)
                                                       cacCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCGCGCTGATTACATTGCGCTGTATAAACGGCAGCACCGAt                                                                                              >  2:148785/1‑150 (MQ=255)
                                                       cacCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGAt                                                                                              >  1:938406/1‑150 (MQ=255)
                                                       cacCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGAt                                                                                              >  1:900532/1‑150 (MQ=255)
                                                       cacCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGAt                                                                                              >  2:943127/1‑150 (MQ=255)
                                                         ccATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGa                                                                                            >  1:181489/1‑150 (MQ=255)
                                                          cATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGaa                                                                                           >  2:1086939/1‑150 (MQ=255)
                                                            tGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACg                                                                                         <  1:507324/150‑1 (MQ=255)
                                                            tGATAGCGCACAATGGCTGGCTACAACACCGCTGCTTCCCAATCACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCACGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACg                                                                                         <  2:388040/150‑1 (MQ=255)
                                                                aGCGCCCAATGGCTGGCGACAAGACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGGCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGcccc                                                                                     <  2:1108750/150‑1 (MQ=255)
                                                                    cccAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATc                                                                                 >  1:805988/1‑150 (MQ=255)
                                                                       aaTGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAAc                                                                              <  2:96738/150‑1 (MQ=255)
                                                                        aTGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACt                                                                             <  1:790597/150‑1 (MQ=255)
                                                                        aTGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACt                                                                             <  2:733343/150‑1 (MQ=255)
                                                                         tggctggcGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTg                                                                            >  1:61894/1‑150 (MQ=255)
                                                                                 gACAACACAGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCAt                                                                    >  2:390111/1‑150 (MQ=255)
                                                                                   caacaCCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGc                                                                  >  1:815607/1‑150 (MQ=255)
                                                                                    aacaCCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCg                                                                 <  2:61894/150‑1 (MQ=255)
                                                                                       acCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCgtgt                                                              >  2:335583/1‑150 (MQ=255)
                                                                                         cGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGTCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGtat                                                            <  2:1148343/150‑1 (MQ=255)
                                                                                         cGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGtat                                                            <  1:535703/150‑1 (MQ=255)
                                                                                            tgctTCCCAATAACGTACGTTATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGtatata                                                         <  2:451404/150‑1 (MQ=255)
                                                                                             gctTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTatatat                                                        >  2:735887/1‑150 (MQ=255)
                                                                                                tCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAACGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTGGCCATGCGTGTATATATGGa                                                     >  2:1432083/1‑150 (MQ=255)
                                                                                                 ccaaaaaaCGTACGTGATGCGATGGCGGGCCAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGAATACATTGCGCTCTATAAAAGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGAc                                                    >  2:610673/7‑150 (MQ=255)
                                                                                                    aaTAACGTACGGGTTGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACggg                                                 <  2:238025/150‑1 (MQ=255)
                                                                                                     aTAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCGCGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGc                                                <  1:148785/150‑1 (MQ=255)
                                                                                                            acgtGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAAt                                         >  1:87818/1‑150 (MQ=255)
                                                                                                             cgtGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAAtt                                        <  1:994427/150‑1 (MQ=255)
                                                                                                               tGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGt                                      <  1:405244/150‑1 (MQ=255)
                                                                                                                  tGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTc                                   <  1:943127/150‑1 (MQ=255)
                                                                                                                   gcgcTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCg                                  <  1:390111/150‑1 (MQ=255)
                                                                                                                        ggcgggcgAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCg                             >  1:694032/1‑150 (MQ=255)
                                                                                                                              cgAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGTCGGTCGGTTAATTGTTTCTACCCGACAACg                       <  2:1519742/150‑1 (MQ=255)
                                                                                                                                   gCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAg                  >  1:324436/1‑150 (MQ=255)
                                                                                                                                    cACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGt                 <  1:956031/150‑1 (MQ=255)
                                                                                                                                       gcgTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCt              <  1:187697/150‑1 (MQ=255)
                                                                                                                                       ccttcaccGAGACGGCTGACTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCt              >  1:874920/7‑150 (MQ=255)
                                                                                                                                       ccttcaccGAGACGGCTGACTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCt              >  2:1362657/7‑150 (MQ=255)
                                                                                                                                       ccttcaccGAGACGGCTGACTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCt              >  2:256257/7‑150 (MQ=255)
                                                                                                                                         ttcaccGAGACGGCTGACTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTgg            <  1:256257/146‑1 (MQ=255)
                                                                                                                                         ttcaccGAGACGGCTGACTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTgg            <  1:1362657/146‑1 (MQ=255)
                                                                                                                                          tcaccGAGACGGCTGACTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGgc           >  2:690565/4‑150 (MQ=255)
                                                                                                                                          tCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGTCGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGgc           <  2:938406/150‑1 (MQ=255)
                                                                                                                                           ccccGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGgcg          >  2:544116/1‑150 (MQ=255)
                                                                                                                                              cGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTg       <  1:735887/150‑1 (MQ=255)
                                                                                                                                                   cAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGa  >  2:76508/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAATACAGATGTGATTGATGAAGCGCATCCCTGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCGCTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGCCGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGCCCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGA  >  NC_000913/1408139‑1408435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑