BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,491,904	A→T	6.0%	intergenic (+54/+18)	ydcA → / ← hokB	protein YdcA/toxin HokB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,491,904	0	A	T	6.0%	318.1 / 7.4	99	intergenic (+54/+18)	ydcA/hokB	protein YdcA/toxin HokB
Reads supporting (aligned to +/- strand): ref base A (48/45); new base T (3/3); total (51/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 103 total aligned reads displayed.

GGTTCGAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATGAGCAGACACACCAC  >  NC_000913/1491755‑1492053

ggTTCGAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGaa                                                                                                                                                       >  1:459788/1‑150 (MQ=255)

ggTTCGAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATCATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGaa                                                                                                                                                       >  1:551266/1‑150 (MQ=255)

  ttCGAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGaaaa                                                                                                                                                     >  1:91996/1‑150 (MQ=255)

    cGAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATc                                                                                                                                                   >  2:947569/1‑150 (MQ=255)

    cGAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATc                                                                                                                                                   >  2:1481037/1‑150 (MQ=255)

      aaaGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATccc                                                                                                                                                 >  1:1135646/1‑150 (MQ=255)

       aaGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATcccc                                                                                                                                                <  1:453166/150‑1 (MQ=255)

         ggtgggATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGATTTTCCCcgc                                                                                                                                              <  2:883009/147‑1 (MQ=255)

          gggggATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGATTTTCCCcgcg                                                                                                                                             <  1:596316/150‑1 (MQ=255)

          gggggATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCcgcg                                                                                                                                             >  2:1009993/1‑150 (MQ=255)

          gggggATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCcgcg                                                                                                                                             <  2:107791/150‑1 (MQ=255)

            gggATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCt                                                                                                                                           >  1:1599364/1‑150 (MQ=255)

             ggATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCtt                                                                                                                                          >  2:360559/1‑150 (MQ=255)

               aTCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGc                                                                                                                                        <  1:1402106/150‑1 (MQ=255)

                tctcACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGcc                                                                                                                                       <  1:1192904/150‑1 (MQ=255)

                   cacaCTGTACGGCAGGCGGCAAATTTGTTTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCtt                                                                                                                                    >  2:797490/1‑150 (MQ=255)

                   cacaCTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCtt                                                                                                                                    >  2:541420/1‑150 (MQ=255)

                   cacaCTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCtt                                                                                                                                    >  2:462467/1‑150 (MQ=255)

                      acTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTAcc                                                                                                                                 <  2:1345435/150‑1 (MQ=255)

                       cTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCt                                                                                                                                >  1:191210/1‑150 (MQ=255)

                         gTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTgg                                                                                                                              <  1:982736/150‑1 (MQ=255)

                          tagggCAGGCGGCTAATTTGTCTGTAATGATGGTTCTATTTGTGGATCGAAAAAAACATGCAGTAAGTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGa                                                                                                                             <  1:1310586/147‑1 (MQ=255)

                           aCGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGAc                                                                                                                            >  1:1579730/1‑150 (MQ=255)

                           aCGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCACTTACCTGGAc                                                                                                                            >  2:55922/1‑150 (MQ=255)

                                  gcggcAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGATGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAgg                                                                                                                     >  1:997856/1‑150 (MQ=255)

                                      cAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCAt                                                                                                                 >  2:1196503/1‑150 (MQ=255)

                                         aTTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAg                                                                                                              <  2:305723/150‑1 (MQ=255)

                                            tGTCTGTAATGATGGTTCTATTAGTACATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAgcgc                                                                                                           >  1:449537/1‑150 (MQ=255)

                                               cTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCgcagc                                                                                                        >  2:1305566/1‑150 (MQ=255)

                                               cTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCgcagc                                                                                                        >  1:373837/1‑150 (MQ=255)

                                               cTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCgcagc                                                                                                        <  1:1305566/150‑1 (MQ=255)

                                                   aaTGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAAcc                                                                                                    <  2:372085/150‑1 (MQ=255)

                                                   aaTGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAAcc                                                                                                    >  1:930214/1‑150 (MQ=255)

                                                         ggTTCTATTAGTGCATCGAAAAAAGCATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTa                                                                                              >  1:941058/1‑150 (MQ=255)

                                                           ttCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATc                                                                                            >  2:1354007/1‑150 (MQ=255)

                                                           ttCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATc                                                                                            >  2:1034061/1‑150 (MQ=255)

                                                              tATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCAcc                                                                                         <  2:459788/150‑1 (MQ=255)

                                                                  aGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGc                                                                                                                        <  1:701476/115‑1 (MQ=255)

                                                                  aGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGc                                                                                                                        >  2:701476/1‑115 (MQ=255)

                                                                  aGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTcc                                                                                     <  2:401352/150‑1 (MQ=255)

                                                                   gTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTccc                                                                                    >  2:115195/1‑150 (MQ=255)

                                                                      cATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGa                                                                                 <  2:930214/150‑1 (MQ=255)

                                                                      cATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGa                                                                                 >  2:883871/1‑150 (MQ=255)

                                                                       aTCGAAAAAACCATGCACTAACTGAAGTGTAAAGGGGGGGCCAGGAGAAAATGAAGCTGCTAGGCAAATCCGCGGGGAAAAGCCCCGCGCTTGCCCTAACCTGAACTGGCAGGCCAGGAGCGCCGCACCCGCCTTAGCACCGGCCCGGac                                                                                >  2:1622354/1‑149 (MQ=255)

                                                                        tCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAAc                                                                               <  2:380692/150‑1 (MQ=255)

                                                                        tCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAAc                                                                               <  1:462467/150‑1 (MQ=255)

                                                                           aaaaaaaCATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGc                                                                            <  1:610095/150‑1 (MQ=255)

                                                                             aaaaaCATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAg                                                                          <  2:1135646/150‑1 (MQ=255)

                                                                                  cATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGt                                                                     <  1:797666/150‑1 (MQ=255)

                                                                                   aTGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTa                                                                    <  2:191210/150‑1 (MQ=255)

                                                                                     gCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTaga                                                                  >  2:1026389/1‑150 (MQ=255)

                                                                                     gCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTaga                                                                  >  2:311106/1‑150 (MQ=255)

                                                                                           aCTGAAGTCTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCGGGGCTTGCCCTTACCTGGACGCGCAGGCCCTGCGCGCAGCAACCTCCTTATCACCGTCCGCGAACCGCAGTTCGGAGAGCGctt                                                            <  2:578309/150‑1 (MQ=255)

                                                                                              gAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCACCCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTc                                                         >  1:112104/1‑150 (MQ=255)

                                                                                              gAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTc                                                         >  2:1491314/1‑150 (MQ=255)

                                                                                                aGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCgg                                                       <  1:262970/150‑1 (MQ=255)

                                                                                                 gtgtAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCggg                                                      >  1:626559/1‑150 (MQ=255)

                                                                                                  tgtAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGt                                                     >  2:651866/1‑150 (MQ=255)

                                                                                                  tgtAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGt                                                     >  2:1102715/1‑150 (MQ=255)

                                                                                                    tAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCACCCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCa                                                   >  1:723474/1‑150 (MQ=255)

                                                                                                     aaaaGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGAGCGGGGAAAATCCCAGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAg                                                  >  1:1324469/1‑150 (MQ=255)

                                                                                                      aaaGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAgg                                                 <  1:1403496/150‑1 (MQ=255)

                                                                                                      aaaGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAgg                                                 <  1:947569/150‑1 (MQ=255)

                                                                                                      aaaGGGGTGCCATGAGAAAATGAATCTGCTAGGCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCATGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAgg                                                 <  2:896981/150‑1 (MQ=255)

                                                                                                       aaGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGa                                                >  2:1333107/1‑150 (MQ=255)

                                                                                                          gggTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAgtg                                             <  2:1346779/150‑1 (MQ=255)

                                                                                                          gggTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAgtg                                             <  1:1009993/150‑1 (MQ=255)

                                                                                                            gTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGtgtg                                           <  1:561486/150‑1 (MQ=255)

                                                                                                              gCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGaa                                         >  2:1595924/1‑150 (MQ=255)

                                                                                                               ccATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAAt                                        <  2:373837/150‑1 (MQ=255)

                                                                                                               ccATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAAt                                        >  2:459080/1‑150 (MQ=255)

                                                                                                               ccATGAGAAAATGAAACTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGACCCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAAt                                        >  2:529578/1‑150 (MQ=255)

                                                                                                                cATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAGTg                                       <  2:1359149/150‑1 (MQ=255)

                                                                                                                cATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATg                                       <  1:1354007/150‑1 (MQ=255)

                                                                                                                 aTGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGt                                      >  1:579436/1‑150 (MQ=255)

                                                                                                                   gagaAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCa                                    <  2:579436/150‑1 (MQ=255)

                                                                                                                    agaAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAg                                   <  2:1240244/150‑1 (MQ=255)

                                                                                                                    agaAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAg                                   >  1:315298/1‑150 (MQ=255)

                                                                                                                       aaaTGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAAt                                <  2:435356/150‑1 (MQ=255)

                                                                                                                         aTGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCg                              <  1:1489631/150‑1 (MQ=255)

                                                                                                                          tGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGt                             <  1:115195/150‑1 (MQ=255)

                                                                                                                           gAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTa                            <  2:941058/150‑1 (MQ=255)

                                                                                                                            aaTCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTc                                                    >  1:1544526/1‑125 (MQ=255)

                                                                                                                            aaTCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTc                                                    <  2:1544526/125‑1 (MQ=255)

                                                                                                                              tCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATg                         <  1:360559/150‑1 (MQ=255)

                                                                                                                                 gCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAg                      <  1:1034061/150‑1 (MQ=255)

                                                                                                                                 gCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAg                      >  2:411654/1‑150 (MQ=255)

                                                                                                                                   tAGTCAAATGCGCGGGGAATATCGCGGCGCTTGCGCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGAt                    <  2:551266/150‑1 (MQ=255)

                                                                                                                                   tAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGAt                    >  2:1401494/1‑150 (MQ=255)

                                                                                                                                       cAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATg                >  2:936128/1‑150 (MQ=255)

                                                                                                                                         caagcgcGGGGATTTTCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATGAg              >  2:1229084/4‑150 (MQ=255)

                                                                                                                                          aTGCGCGTGGAAAATCCCCGCGCTTGCCCTTACCTGCACGTCCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACAGCAGTTCGTAGAGCGTTTGTACGCTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATGAGc             >  2:808169/1‑150 (MQ=255)

                                                                                                                                          aTGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATGAGc             <  1:1089736/150‑1 (MQ=255)

                                                                                                                                           agcgcGGGGATTTTCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATGAGCa            >  2:611906/2‑150 (MQ=255)

                                                                                                                                            gcgcGGGGATTTTCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATg                                       <  2:755679/122‑1 (MQ=255)

                                                                                                                                            gcgcGGGGATTTTCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATg                                       >  1:755679/1‑122 (MQ=255)

                                                                                                                                             cgcgGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATGAGCAGa          <  1:541420/150‑1 (MQ=255)

                                                                                                                                              gcgGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATGAGCAGac         <  1:311106/150‑1 (MQ=255)

                                                                                                                                                 gggAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAAAGCAGATAATGAGCAGacacc      >  2:637622/1‑149 (MQ=255)

                                                                                                                                                     aaaTCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATGAGCAGACAcaccac  >  2:904324/1‑150 (MQ=255)

GGTTCGAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATGAGCAGACACACCAC  >  NC_000913/1491755‑1492053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`