Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,720,364 G→T 7.9% intergenic (+21/+26) slyB → / ← slyA outer membrane lipoprotein SlyB/DNA‑binding transcriptional dual regulator SlyA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,720,3640GT 7.9% 270.6 / 8.2 90intergenic (+21/+26)slyB/slyAouter membrane lipoprotein SlyB/DNA‑binding transcriptional dual regulator SlyA
Reads supporting (aligned to +/- strand):  ref base G (41/41);  new base T (4/4);  total (45/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCT  >  NC_000913/1720215‑1720513
                                                                                                                                                     |                                                                                                                                                     
cTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAgg                                                                                                                                                       <  2:1159710/150‑1 (MQ=255)
cTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAgg                                                                                                                                                       >  1:340023/1‑150 (MQ=255)
  ggAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGACGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGAGCTAAATAAGTTTGCGTGTGGTCAGGtt                                                                                                                                                     >  2:65712/1‑150 (MQ=255)
   gAAATTCGTAAAGACGATGGTAATACCATCATGCTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTAAGGTTa                                                                                                                                                    >  2:1481545/1‑150 (MQ=255)
     aaTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACt                                                                                                                                                  <  1:1271590/150‑1 (MQ=255)
      aTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTg                                                                                                                                                 <  2:566533/150‑1 (MQ=255)
      aTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTg                                                                                                                                                 <  2:1243959/150‑1 (MQ=255)
         cGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGAcc                                                                                                                                              <  2:528897/150‑1 (MQ=255)
           tataGACGCTGGTATTACGATCATGGTGGTTCAGAAACAAGGCAACTCTCGTTTCTCTCCGGGCGAACGTGTGGTTCTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATCAGTTTGGGTGTGGTCTGGTTACTGAacac                                                                                                                                            <  2:975028/147‑1 (MQ=255)
           tAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTAc                                                                                                                                                   <  2:324553/143‑1 (MQ=255)
           tAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTAc                                                                                                                                                   >  1:324553/1‑143 (MQ=255)
            aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGTAACCTGACcaca                                                                                                                                           <  2:1176085/150‑1 (MQ=255)
            aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACcacc                                                                                                                                           >  1:938824/1‑149 (MQ=255)
               gACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGTAACCTGACCACACGc                                                                                                                                        <  1:1281151/150‑1 (MQ=255)
                 cGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACACGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGcac                                                                                                                                      >  1:631736/1‑148 (MQ=255)
                       tattACCATCATGGTGGTACAGAAACAAGGCAACCCTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCa                                                                                                                                >  2:178618/4‑150 (MQ=255)
                             catcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTg                                                                                                                                                 >  2:311028/1‑127 (MQ=255)
                             catcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTg                                                                                                                                                 <  1:311028/127‑1 (MQ=255)
                             catcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAc                                                                                                                          <  2:539522/150‑1 (MQ=255)
                              atcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAcc                                                                                                                         <  2:751149/150‑1 (MQ=255)
                              atcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAcc                                                                                                                         >  1:310631/1‑150 (MQ=255)
                               taatGGTGGTACAGAAACAAGGCAACACTCTTTTCTCTCCGGGCCAACGTGTCGAACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAccc                                                                                                                        <  2:1391803/148‑1 (MQ=255)
                                    gtggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACcaca                                                                                                                                           >  2:433050/1‑126 (MQ=255)
                                    gtggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACcaca                                                                                                                                           <  1:433050/126‑1 (MQ=255)
                                    gtggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTgg                                                                                                                   >  1:1187379/1‑150 (MQ=255)
                                      ggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGGCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGcc                                                                                                                 <  1:1352345/150‑1 (MQ=255)
                                       gtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCt                                                                                                                >  1:937991/1‑150 (MQ=255)
                                        tACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGGCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTg                                                                                                               >  1:216536/1‑150 (MQ=255)
                                         aCAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGt                                                                                                              >  2:351912/1‑150 (MQ=255)
                                          cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGCCCACACGCCCCCTTCATTTCACCCTTTGGCCtata                                                                                                             >  1:1415376/1‑150 (MQ=255)
                                          aacaaaCACGGCAACATTGGTTTCTCTCCGGCCCACGGTGTCTTCGGGGCCAGAAATGGCTTTCTGGTGACCGTTTCTCCGCTATAAATATGTTTGGGTGTGTTCTGGTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                             <  2:428340/147‑1 (MQ=255)
                                           aGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTaa                                                                                                            >  2:951539/1‑150 (MQ=255)
                                            gAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAAc                                                                                                           >  2:440975/1‑150 (MQ=255)
                                                 aaGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAAGGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTCACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAAt                                                                                                      <  2:574320/150‑1 (MQ=255)
                                                   ggCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGa                                                                                                    >  1:1119862/1‑150 (MQ=255)
                                                    gCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGat                                                                                                   >  1:1236359/1‑150 (MQ=255)
                                                     cAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAACTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGata                                                                                                  <  2:1524496/150‑1 (MQ=255)
                                                         acTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCCGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACCCGCCCCCTTCATTTCACCCGTTGGCCTGTAACTCAATGAtattat                                                                                              >  2:129023/1‑150 (MQ=255)
                                                          cTCGTTTCTCTCCGGGCCAAGGTGTCGTACTGGCCAGCAATGGCTGTCTGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATg                                                                                             <  2:340023/150‑1 (MQ=255)
                                                          cTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATg                                                                                             >  2:1358373/1‑150 (MQ=255)
                                                           tCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTGGCGGGGGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGc                                                                                            <  1:117742/150‑1 (MQ=255)
                                                            cGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCt                                                                                           >  1:364436/1‑150 (MQ=255)
                                                               ttCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGCCCCCACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCaa                                                                                        >  1:846572/1‑150 (MQ=255)
                                                                tctctcCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAg                                                                                       >  1:1321257/1‑150 (MQ=255)
                                                                    tcCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGtttt                                                                                   >  1:243073/1‑150 (MQ=255)
                                                                     ccGGGCCAACGTGTCGTACTGGCCATCAATTGCATTCATGTGACCGTTTCTCCGCGATAAAAAATTTTGCGTGTTTTCAGTTTACTGACCACACGCCCCCTTCATTTGACCCTTTTGCCTGTAACTCAATGATATTATGGTCAAGTTTTg                                                                                  <  2:1631875/150‑1 (MQ=255)
                                                                      cGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                 <  2:42657/150‑1 (MQ=255)
                                                                      cGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                 >  1:877658/1‑150 (MQ=255)
                                                                       gggCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCg                                                                                <  1:1210167/150‑1 (MQ=255)
                                                                             aCGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGc                                                                          <  2:861804/150‑1 (MQ=255)
                                                                               gtgtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGt                                                                        >  1:734011/1‑150 (MQ=255)
                                                                                tgtCGTAGGGGCCAGCAATGGCCGTCAGGTCACCGTTTCTCCGCGCTAAATAAGTTGGCGGGTGGGCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTa                                                                       <  1:772788/150‑1 (MQ=255)
                                                                                tgtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTa                                                                       >  2:552988/1‑150 (MQ=255)
                                                                                tgtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGACTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTa                                                                       >  1:365511/1‑150 (MQ=255)
                                                                                    gTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGGGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCa                                                                   <  2:721669/150‑1 (MQ=255)
                                                                                       cTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTTAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGtt                                                                <  1:368442/150‑1 (MQ=255)
                                                                                        tGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTg                                                               >  2:569066/1‑150 (MQ=255)
                                                                                        tGGCCAGCAATGGAAGTCAAGTGACAGTTTCTCCGAGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTg                                                               >  2:487800/1‑150 (MQ=255)
                                                                                            cAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTcc                                                           <  2:317450/150‑1 (MQ=255)
                                                                                            cAGCAATGGCAGGCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGGGTGGGCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTcc                                                           <  1:1424895/150‑1 (MQ=255)
                                                                                             aGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCa                                                          >  2:1484462/1‑150 (MQ=255)
                                                                                                aaTGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGtt                                                       <  1:951539/150‑1 (MQ=255)
                                                                                                   ggCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCct                                                    >  1:156060/1‑150 (MQ=255)
                                                                                                         cAGTTGACCGTTTATCCGCGATAATTATTTTTGGTTGTGGTCTGGTTAGTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTTTAACTCAATGATATTATGATCAAGTTTTGCGATGAGCGTAATCAGTTTCTCCTGTTCCTCTGCgg                                              <  2:631736/150‑1 (MQ=255)
                                                                                                         cAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCgg                                              <  2:1236359/150‑1 (MQ=255)
                                                                                                           ggtcccggTTTCCCCGCGCTAAATATGTTGGGGGGGGGCCCGGTTCCTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGgag                                            <  1:878092/143‑1 (MQ=255)
                                                                                                                  gTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAt                                     <  2:1187379/150‑1 (MQ=255)
                                                                                                                      ctcCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTaa                                 >  1:60794/1‑150 (MQ=255)
                                                                                                                       tcCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAat                                >  2:139/1‑150 (MQ=255)
                                                                                                                       tcCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAat                                >  1:86910/1‑150 (MQ=255)
                                                                                                                       gcggcgcTAAATACTTTTGGTTTTGTTCTGGTTAGTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAat                                <  2:156060/147‑1 (MQ=255)
                                                                                                                               aaaTAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcgc                        <  2:310631/150‑1 (MQ=255)
                                                                                                                                aaTAAGTTTGCTTTTGGACTGTTTCCTTCCCACACGCCCCCTTCATTTGCCGCTTTGGCCTGTAACTCTATGATATTATGCTCAAGTTTTGCGATGAGGGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcgcg                       <  1:1067765/150‑1 (MQ=255)
                                                                                                                                aaTAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcgcg                       >  1:1335212/1‑150 (MQ=255)
                                                                                                                                 aTAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCGTGTAATATTTCcgcgc                      >  1:8619/1‑150 (MQ=255)
                                                                                                                                  tacgTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcgcgcg                     <  1:543733/147‑1 (MQ=255)
                                                                                                                                   acgTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCgg                    <  2:432132/148‑1 (MQ=255)
                                                                                                                                    aGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCggg                   >  1:117509/1‑150 (MQ=255)
                                                                                                                                          cGTGTGGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt             >  1:753818/1‑150 (MQ=255)
                                                                                                                                          cGTGTGGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt             >  2:1526090/1‑150 (MQ=255)
                                                                                                                                          cGTGTGGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt             >  2:154547/1‑150 (MQ=255)
                                                                                                                                          cGTGTGGGCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGAGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt             <  2:1174373/150‑1 (MQ=255)
                                                                                                                                           gtgtGGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGGGATGCCATGTAATATTTCCGCGCGGGTTTTGtt            >  1:1188983/1‑150 (MQ=255)
                                                                                                                                           gtgtGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGtt            <  2:1335212/150‑1 (MQ=255)
                                                                                                                                           gtgtGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGtt            <  2:364436/150‑1 (MQ=255)
                                                                                                                                            tgtgGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTta           <  1:487800/150‑1 (MQ=255)
                                                                                                                                            tgtgGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTta           <  2:734011/150‑1 (MQ=255)
                                                                                                                                            tgtgGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATACCATGTAATATTTCCGCGCGGGTTTTGTta           >  2:785727/1‑150 (MQ=255)
                                                                                                                                               ggTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaata        <  1:351912/150‑1 (MQ=255)
                                                                                                                                                 tCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAAc      >  1:1307774/1‑150 (MQ=255)
                                                                                                                                                  cAGGTTACGTACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACa     <  2:846572/150‑1 (MQ=255)
                                                                                                                                                   aGGTTACAGACCACACGCCCCCTTCATTTCACCCATAGGCCTGTAACTCAATGATATTATGCTCAACTTTTGGCATGAGCTAAATCACTTGCTCAAGATCCACAGCGGATATGCCATGTAAAATTTACGCGCGGGTTTTGTAAAAAACAg    >  2:657222/1‑150 (MQ=255)
                                                                                                                                                    ggTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGc   >  2:977752/1‑150 (MQ=255)
                                                                                                                                                     gTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCt  >  1:936699/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCT  >  NC_000913/1720215‑1720513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑