Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,720,367 A→C 8.6% intergenic (+24/+23) slyB → / ← slyA outer membrane lipoprotein SlyB/DNA‑binding transcriptional dual regulator SlyA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,720,3670AC 8.6% 240.5 / 12.4 91intergenic (+24/+23)slyB/slyAouter membrane lipoprotein SlyB/DNA‑binding transcriptional dual regulator SlyA
Reads supporting (aligned to +/- strand):  ref base A (42/41);  new base C (4/4);  total (46/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.89e-01

GAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTTCC  >  NC_000913/1720218‑1720516
                                                                                                                                                     |                                                                                                                                                     
gAAATTCGTAAAGACGATGGTAATACCATCATGCTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTAAGGTTa                                                                                                                                                       >  2:1481545/1‑150 (MQ=255)
  aaTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACt                                                                                                                                                     <  1:1271590/150‑1 (MQ=255)
   aTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTg                                                                                                                                                    <  2:566533/150‑1 (MQ=255)
   aTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTg                                                                                                                                                    <  2:1243959/150‑1 (MQ=255)
      cGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGAcc                                                                                                                                                 <  2:528897/150‑1 (MQ=255)
        tataGACGCTGGTATTACGATCATGGTGGTTCAGAAACAAGGCAACTCTCGTTTCTCTCCGGGCGAACGTGTGGTTCTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATCAGTTTGGGTGTGGTCTGGTTACTGAacac                                                                                                                                               <  2:975028/147‑1 (MQ=255)
        tAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTAc                                                                                                                                                      >  1:324553/1‑143 (MQ=255)
        tAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTAc                                                                                                                                                      <  2:324553/143‑1 (MQ=255)
         aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGTAACCTGACcaca                                                                                                                                              <  2:1176085/150‑1 (MQ=255)
         aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACcacc                                                                                                                                              >  1:938824/1‑149 (MQ=255)
            gACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGTAACCTGACCACACGc                                                                                                                                           <  1:1281151/150‑1 (MQ=255)
              cGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACACGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGcac                                                                                                                                         >  1:631736/1‑148 (MQ=255)
                    tattACCATCATGGTGGTACAGAAACAAGGCAACCCTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCa                                                                                                                                   >  2:178618/4‑150 (MQ=255)
                          catcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTg                                                                                                                                                    >  2:311028/1‑127 (MQ=255)
                          catcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTg                                                                                                                                                    <  1:311028/127‑1 (MQ=255)
                          catcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAc                                                                                                                             <  2:539522/150‑1 (MQ=255)
                           atcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAcc                                                                                                                            >  1:310631/1‑150 (MQ=255)
                           atcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAcc                                                                                                                            <  2:751149/150‑1 (MQ=255)
                            taatGGTGGTACAGAAACAAGGCAACACTCTTTTCTCTCCGGGCCAACGTGTCGAACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAccc                                                                                                                           <  2:1391803/148‑1 (MQ=255)
                                 gtggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACcaca                                                                                                                                              >  2:433050/1‑126 (MQ=255)
                                 gtggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACcaca                                                                                                                                              <  1:433050/126‑1 (MQ=255)
                                 gtggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTgg                                                                                                                      >  1:1187379/1‑150 (MQ=255)
                                   ggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGGCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGcc                                                                                                                    <  1:1352345/150‑1 (MQ=255)
                                    gtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCt                                                                                                                   >  1:937991/1‑150 (MQ=255)
                                     tACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGGCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTg                                                                                                                  >  1:216536/1‑150 (MQ=255)
                                      aCAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGt                                                                                                                 >  2:351912/1‑150 (MQ=255)
                                       cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGCCCACACGCCCCCTTCATTTCACCCTTTGGCCtata                                                                                                                >  1:1415376/1‑150 (MQ=255)
                                       aacaaaCACGGCAACATTGGTTTCTCTCCGGCCCACGGTGTCTTCGGGGCCAGAAATGGCTTTCTGGTGACCGTTTCTCCGCTATAAATATGTTTGGGTGTGTTCTGGTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                                <  2:428340/147‑1 (MQ=255)
                                        aGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTaa                                                                                                               >  2:951539/1‑150 (MQ=255)
                                         gAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAAc                                                                                                              >  2:440975/1‑150 (MQ=255)
                                              aaGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAAGGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTCACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAAt                                                                                                         <  2:574320/150‑1 (MQ=255)
                                                ggCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGa                                                                                                       >  1:1119862/1‑150 (MQ=255)
                                                 gCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGat                                                                                                      >  1:1236359/1‑150 (MQ=255)
                                                  cAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAACTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGata                                                                                                     <  2:1524496/150‑1 (MQ=255)
                                                      acTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCCGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACCCGCCCCCTTCATTTCACCCGTTGGCCTGTAACTCAATGAtattat                                                                                                 >  2:129023/1‑150 (MQ=255)
                                                       cTCGTTTCTCTCCGGGCCAAGGTGTCGTACTGGCCAGCAATGGCTGTCTGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATg                                                                                                <  2:340023/150‑1 (MQ=255)
                                                       cTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATg                                                                                                >  2:1358373/1‑150 (MQ=255)
                                                        tCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTGGCGGGGGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGc                                                                                               <  1:117742/150‑1 (MQ=255)
                                                         cGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCt                                                                                              >  1:364436/1‑150 (MQ=255)
                                                            ttCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGCCCCCACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCaa                                                                                           >  1:846572/1‑150 (MQ=255)
                                                             tctctcCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAg                                                                                          >  1:1321257/1‑150 (MQ=255)
                                                                 tcCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGtttt                                                                                      >  1:243073/1‑150 (MQ=255)
                                                                  ccGGGCCAACGTGTCGTACTGGCCATCAATTGCATTCATGTGACCGTTTCTCCGCGATAAAAAATTTTGCGTGTTTTCAGTTTACTGACCACACGCCCCCTTCATTTGACCCTTTTGCCTGTAACTCAATGATATTATGGTCAAGTTTTg                                                                                     <  2:1631875/150‑1 (MQ=255)
                                                                   cGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                    >  1:877658/1‑150 (MQ=255)
                                                                   cGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                    <  2:42657/150‑1 (MQ=255)
                                                                    gggCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCg                                                                                   <  1:1210167/150‑1 (MQ=255)
                                                                          aCGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGc                                                                             <  2:861804/150‑1 (MQ=255)
                                                                            gtgtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGt                                                                           >  1:734011/1‑150 (MQ=255)
                                                                             tgtCGTAGGGGCCAGCAATGGCCGTCAGGTCACCGTTTCTCCGCGCTAAATAAGTTGGCGGGTGGGCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTa                                                                          <  1:772788/150‑1 (MQ=255)
                                                                             tgtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTa                                                                          >  2:552988/1‑150 (MQ=255)
                                                                             tgtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGACTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTa                                                                          >  1:365511/1‑150 (MQ=255)
                                                                                 gTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGGGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCa                                                                      <  2:721669/150‑1 (MQ=255)
                                                                                    cTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTTAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGtt                                                                   <  1:368442/150‑1 (MQ=255)
                                                                                     tGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTg                                                                  >  2:569066/1‑150 (MQ=255)
                                                                                     tGGCCAGCAATGGAAGTCAAGTGACAGTTTCTCCGAGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTg                                                                  >  2:487800/1‑150 (MQ=255)
                                                                                         cAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTcc                                                              <  2:317450/150‑1 (MQ=255)
                                                                                         cAGCAATGGCAGGCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGGGTGGGCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTcc                                                              <  1:1424895/150‑1 (MQ=255)
                                                                                          aGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCa                                                             >  2:1484462/1‑150 (MQ=255)
                                                                                             aaTGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGtt                                                          <  1:951539/150‑1 (MQ=255)
                                                                                                ggCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCct                                                       >  1:156060/1‑150 (MQ=255)
                                                                                                      cAGTTGACCGTTTATCCGCGATAATTATTTTTGGTTGTGGTCTGGTTAGTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTTTAACTCAATGATATTATGATCAAGTTTTGCGATGAGCGTAATCAGTTTCTCCTGTTCCTCTGCgg                                                 <  2:631736/150‑1 (MQ=255)
                                                                                                      cAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCgg                                                 <  2:1236359/150‑1 (MQ=255)
                                                                                                        ggtcccggTTTCCCCGCGCTAAATATGTTGGGGGGGGGCCCGGTTCCTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGgag                                               <  1:878092/143‑1 (MQ=255)
                                                                                                               gTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAt                                        <  2:1187379/150‑1 (MQ=255)
                                                                                                                   ctcCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTaa                                    >  1:60794/1‑150 (MQ=255)
                                                                                                                    tcCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAat                                   >  1:86910/1‑150 (MQ=255)
                                                                                                                    tcCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAat                                   >  2:139/1‑150 (MQ=255)
                                                                                                                    gcggcgcTAAATACTTTTGGTTTTGTTCTGGTTAGTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAat                                   <  2:156060/147‑1 (MQ=255)
                                                                                                                            aaaTAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcgc                           <  2:310631/150‑1 (MQ=255)
                                                                                                                             aaTAAGTTTGCTTTTGGACTGTTTCCTTCCCACACGCCCCCTTCATTTGCCGCTTTGGCCTGTAACTCTATGATATTATGCTCAAGTTTTGCGATGAGGGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcgcg                          <  1:1067765/150‑1 (MQ=255)
                                                                                                                             aaTAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcgcg                          >  1:1335212/1‑150 (MQ=255)
                                                                                                                              aTAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCGTGTAATATTTCcgcgc                         >  1:8619/1‑150 (MQ=255)
                                                                                                                               tacgTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcgcgcg                        <  1:543733/147‑1 (MQ=255)
                                                                                                                                acgTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCgg                       <  2:432132/148‑1 (MQ=255)
                                                                                                                                 aGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCggg                      >  1:117509/1‑150 (MQ=255)
                                                                                                                                       cGTGTGGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt                >  2:1526090/1‑150 (MQ=255)
                                                                                                                                       cGTGTGGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt                >  2:154547/1‑150 (MQ=255)
                                                                                                                                       cGTGTGGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt                >  1:753818/1‑150 (MQ=255)
                                                                                                                                       cGTGTGGGCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGAGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt                <  2:1174373/150‑1 (MQ=255)
                                                                                                                                        gtgtGGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGGGATGCCATGTAATATTTCCGCGCGGGTTTTGtt               >  1:1188983/1‑150 (MQ=255)
                                                                                                                                        gtgtGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGtt               <  2:1335212/150‑1 (MQ=255)
                                                                                                                                        gtgtGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGtt               <  2:364436/150‑1 (MQ=255)
                                                                                                                                         tgtgGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTta              <  1:487800/150‑1 (MQ=255)
                                                                                                                                         tgtgGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTta              <  2:734011/150‑1 (MQ=255)
                                                                                                                                         tgtgGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATACCATGTAATATTTCCGCGCGGGTTTTGTta              >  2:785727/1‑150 (MQ=255)
                                                                                                                                            ggTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaata           <  1:351912/150‑1 (MQ=255)
                                                                                                                                              tcaccttACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAAACAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAAc         >  2:825142/6‑150 (MQ=255)
                                                                                                                                              tCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAAc         >  1:1307774/1‑150 (MQ=255)
                                                                                                                                               cAGGTTACGTACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACa        <  2:846572/150‑1 (MQ=255)
                                                                                                                                                aGGTTACAGACCACACGCCCCCTTCATTTCACCCATAGGCCTGTAACTCAATGATATTATGCTCAACTTTTGGCATGAGCTAAATCACTTGCTCAAGATCCACAGCGGATATGCCATGTAAAATTTACGCGCGGGTTTTGTAAAAAACAg       >  2:657222/1‑150 (MQ=255)
                                                                                                                                                 ggTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGc      >  2:977752/1‑150 (MQ=255)
                                                                                                                                                  gTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCt     >  1:936699/1‑150 (MQ=255)
                                                                                                                                                     aCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGGAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTTcc  >  1:1410554/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTTCC  >  NC_000913/1720218‑1720516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑