Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,737,439 G→T 7.9% intergenic (‑149/+17) ydhP ← / ← ynhF putative transporter YdhP/stress response membrane protein YnhF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,737,4390GT 7.9% 333.0 / 12.1 101intergenic (‑149/+17)ydhP/ynhFputative transporter YdhP/stress response membrane protein YnhF
Reads supporting (aligned to +/- strand):  ref base G (43/50);  new base T (4/4);  total (47/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

Only 100 of 103 total aligned reads displayed.

TGCCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGAT  >  NC_000913/1737290‑1737583
                                                                                                                                                     |                                                                                                                                                
tGCCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAAcgcg                                                                                                                                                  >  1:653817/1‑150 (MQ=255)
 gCCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACgcgc                                                                                                                                                 <  2:973163/150‑1 (MQ=255)
  ccTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCt                                                                                                                                                >  1:1246859/1‑150 (MQ=255)
       actcCACCTCTTCGCGTCATTACGCGATAATCAATAAAGGGGCGAAAGGATGACATCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGCGCGTTCTCCCTc                                                                                                                                           <  2:1243311/149‑1 (MQ=255)
        ctcCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGCGCGTTCTCCCTCg                                                                                                                                          <  2:141964/150‑1 (MQ=255)
        ctcCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCg                                                                                                                                          >  2:614141/1‑150 (MQ=255)
        ctcCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCg                                                                                                                                          <  2:15978/150‑1 (MQ=255)
         tcCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGCGCGTTCTCCCTCgc                                                                                                                                         <  1:1471203/150‑2 (MQ=255)
         tcCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCga                                                                                                                                         >  2:1386860/1‑150 (MQ=255)
          ccACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGGGAGGGAGAACGCGCTCCCTCgag                                                                                                                                        <  2:463074/150‑1 (MQ=255)
          ccACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGCGCGTTCTCCCTCgca                                                                                                                                        <  1:375421/150‑3 (MQ=255)
          ccACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgag                                                                                                                                        <  2:468581/150‑1 (MQ=255)
          ccACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgag                                                                                                                                        >  2:894881/1‑150 (MQ=255)
           cACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgaga                                                                                                                                       <  2:1095867/150‑1 (MQ=255)
           cACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgaga                                                                                                                                       <  2:128875/150‑1 (MQ=255)
           cACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgaga                                                                                                                                       <  1:239852/150‑1 (MQ=255)
           cACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgaga                                                                                                                                       >  1:204910/1‑150 (MQ=255)
             gaatatCGCGTAATGACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAgg                                                                                                                                     >  1:1138056/6‑150 (MQ=255)
                     cgTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGt                                                                                                                             <  2:12276/150‑1 (MQ=255)
                        cATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCa                                                                                                                          >  1:321351/1‑150 (MQ=255)
                              gcgATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCgg                                                                                                                    >  1:1182641/1‑150 (MQ=255)
                               cgATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAGACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGc                                                                                                                   <  2:1060574/150‑1 (MQ=255)
                               cgATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGc                                                                                                                   >  2:278010/1‑150 (MQ=255)
                               cgATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGc                                                                                                                   <  1:1327037/150‑1 (MQ=255)
                                gATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCTATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCa                                                                                                                  >  2:426573/1‑150 (MQ=255)
                                 atatTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCTATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAg                                                                                                                 <  1:426573/150‑1 (MQ=255)
                                   atTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTc                                                                                                               <  2:778926/150‑1 (MQ=255)
                                    ttCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCa                                                                                                              <  1:861333/150‑1 (MQ=255)
                                      cATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAGACCCTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCaac                                                                                                            >  1:1613699/1‑149 (MQ=255)
                                      cATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGAGCGGCAGTCaaa                                                                                                            >  1:375618/1‑150 (MQ=255)
                                          aaaGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGagag                                                                                                                                      >  2:576743/1‑120 (MQ=255)
                                          aaaGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGagag                                                                                                                                      <  1:576743/120‑1 (MQ=255)
                                          aaaGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCa                                                                                                        >  1:1277912/1‑150 (MQ=255)
                                          aaaGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCa                                                                                                        >  1:22144/1‑150 (MQ=255)
                                            attgtcGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAATGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGTAAATCAGTGCAGCGCGGCAGTCAAACCCACg                                                                                                      <  2:1445191/145‑1 (MQ=255)
                                            aGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACg                                                                                                      >  1:1080883/1‑150 (MQ=255)
                                            aGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACg                                                                                                      <  1:411571/150‑1 (MQ=255)
                                             gTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACgg                                                                                                     >  1:858010/1‑150 (MQ=255)
                                               ggCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCt                                                                                                   <  1:366342/150‑1 (MQ=255)
                                                     aGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATc                                                                                             <  1:1146994/150‑1 (MQ=255)
                                                      gCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCa                                                                                            >  2:1546827/1‑150 (MQ=255)
                                                       cATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCaa                                                                                           >  2:163311/1‑150 (MQ=255)
                                                        aTGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCaaa                                                                                          >  2:1257813/1‑150 (MQ=255)
                                                         tGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAAc                                                                                         <  2:1138056/150‑1 (MQ=255)
                                                          gACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGc                                                                                                    >  1:1514793/1‑138 (MQ=255)
                                                           aCAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCg                                                                                       >  2:1320622/1‑150 (MQ=255)
                                                           aCAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCg                                                                                       >  1:501031/1‑150 (MQ=255)
                                                             aGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGAAGTCAAACCCACGGCTACGATCAAAACGAg                                                                                     >  2:679501/1‑150 (MQ=255)
                                                               cAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGACCGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCCCGGCTACGAGCAAACCGAGGa                                                                                   >  2:1368436/1‑150 (MQ=255)
                                                                aaTCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGAc                                                                                  <  1:1366701/150‑1 (MQ=255)
                                                                       aaaaaaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGAGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATc                                                                           >  1:200558/1‑150 (MQ=255)
                                                                           aaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgttg                                                                       <  2:200558/150‑1 (MQ=255)
                                                                           aaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGGCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgttg                                                                       <  2:1087379/150‑1 (MQ=255)
                                                                                aaaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTAcc                                                                  <  1:806831/150‑1 (MQ=255)
                                                                                aaaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTAcc                                                                  <  2:937684/150‑1 (MQ=255)
                                                                                aaaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTAcc                                                                  <  1:656445/150‑1 (MQ=255)
                                                                                aaaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTAcc                                                                  <  1:894881/150‑1 (MQ=255)
                                                                                  aTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAg                                                                <  1:1546827/150‑1 (MQ=255)
                                                                                   tAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGt                                                               <  1:996397/150‑1 (MQ=255)
                                                                                      cAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGaa                                                            >  1:672250/1‑150 (MQ=255)
                                                                                          aaGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAAtt                                                        <  2:501031/150‑1 (MQ=255)
                                                                                                aaaCACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGt                                                  <  1:1095398/150‑1 (MQ=255)
                                                                                                 aaCACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTc                                                 <  2:672250/150‑1 (MQ=255)
                                                                                                 aaCACTTTTGCCATTTTGCTAACAAACACGAAGCAGATGCGAGGGAGAACGCGATCCCACGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTc                                                 >  2:384000/1‑150 (MQ=255)
                                                                                                   cacTTTTGCCATTTTGCTGACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCgg                                               >  1:342705/1‑150 (MQ=255)
                                                                                                         tGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtca                                         <  2:898177/150‑1 (MQ=255)
                                                                                                          gCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtcat                                        >  2:334436/1‑150 (MQ=255)
                                                                                                           ccATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtcatc                                       >  2:520908/1‑150 (MQ=255)
                                                                                                           ccATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtcatc                                       >  1:1627231/1‑150 (MQ=255)
                                                                                                                tttcTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGtt                                  <  2:376173/147‑1 (MQ=255)
                                                                                                                 tGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGttt                                 <  1:1055060/150‑1 (MQ=255)
                                                                                                                   cTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtc                               <  1:814347/150‑1 (MQ=255)
                                                                                                                   cTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtc                               >  2:588183/1‑150 (MQ=255)
                                                                                                                         aacaGGACGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCttt                         <  2:1143115/150‑1 (MQ=255)
                                                                                                                         aacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCttt                         <  2:114190/150‑1 (MQ=255)
                                                                                                                         aacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCttt                         <  2:572805/150‑1 (MQ=255)
                                                                                                                          acaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGtt                                  <  2:110905/140‑1 (MQ=255)
                                                                                                                          acaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGtt                                  >  1:110905/1‑140 (MQ=255)
                                                                                                                             ggaaggaGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTtta                     <  2:22144/150‑1 (MQ=255)
                                                                                                                             ggaaggaGATGCGAGGGAGAACGCGATCCCTCGATAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATAAAACCGAGGACGATAAGCGTTGTTACAAGTGAAAATTTAAGGACGGTGCTCATCAAGTTTTAACCTTTTtta                     >  2:451261/1‑150 (MQ=255)
                                                                                                                               aaggaGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTttatt                   <  2:197728/150‑1 (MQ=255)
                                                                                                                                  tcctctcGAGGGAGCGCGTTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAACCCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTAcc                >  1:1618232/7‑150 (MQ=255)
                                                                                                                                  gagaTGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTAcc                <  2:1182641/150‑1 (MQ=255)
                                                                                                                                     tctcGAGGGAGCGCGTTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACcaca             >  1:169181/4‑150 (MQ=255)
                                                                                                                                     tctcGAGGGAGCGCGTTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACcaca             >  2:63310/4‑150 (MQ=255)
                                                                                                                                      tGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACcacac            >  1:955596/1‑150 (MQ=255)
                                                                                                                                      tGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACcacac            <  2:321351/150‑1 (MQ=255)
                                                                                                                                       gCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACcacaca           >  2:927982/1‑150 (MQ=255)
                                                                                                                                        cGAGGGAGCGCGTTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACaa          >  2:1048570/1‑150 (MQ=255)
                                                                                                                                         gAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACaaa         >  2:1143073/1‑150 (MQ=255)
                                                                                                                                         gAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACaaa         >  2:762890/1‑150 (MQ=255)
                                                                                                                                         gAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACaaa         >  2:757199/1‑150 (MQ=255)
                                                                                                                                           gggAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACaaaaa       >  1:1082466/1‑150 (MQ=255)
                                                                                                                                              agaACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTg    <  1:163311/150‑1 (MQ=255)
                                                                                                                                               gaACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCTTTTTTTATTAc                 >  1:120445/1‑136 (MQ=255)
                                                                                                                                               gaACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCTTTTTTTATTAc                 <  2:120445/136‑1 (MQ=255)
                                                                                                                                               gaACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGa   <  1:1432346/150‑1 (MQ=255)
                                                                                                                                               gaACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGa   <  1:1386860/150‑1 (MQ=255)
                                                                                                                                               gaACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGa   <  1:278010/150‑1 (MQ=255)
                                                                                                                                                aaCGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGat  <  2:863148/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
TGCCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGAT  >  NC_000913/1737290‑1737583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑