BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,632	T→A	V107E (GTG→GAG)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,632	0	T	A	98.5%	720.3 / 1.1	197	V107E (GTG→GAG)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand): ref base T (1/2); new base A (91/103); total (92/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01

Only 100 of 198 total aligned reads displayed.

CTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGA  >  NC_000913/3041535‑3041727

  gTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGAcc                                                                                             <  1:4397361/100‑1 (MQ=255)

  gTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGAcc                                                                                             >  1:1784296/1‑100 (MQ=255)

    tCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCAt                                                                                           >  1:4305400/1‑100 (MQ=255)

        cgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATcgcg                                                                                       <  1:2733464/100‑1 (MQ=255)

         gacgacGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCgcgc                                                                                      >  1:223543/1‑100 (MQ=255)

          aCGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGcc                                                                                     >  1:554244/1‑100 (MQ=255)

            ggCGATGGCTTTGCATGGAGTGAACGGCGGAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGAc                                                                                              <  2:2603566/89‑1 (MQ=255)

              cGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCAAAAAAAAAAAAAAAAATATGCGGTATTCTCTAAAGAGACCa                                                                                            <  2:1552975/89‑1 (MQ=255)

                 tGGCTTTGCATGGATTGAACGGCGAAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATcg                                                                                         <  2:3355824/89‑1 (MQ=255)

                  ggCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGacgacg                                                                             <  1:4497377/100‑1 (MQ=255)

                   gCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGAcgacga                                                                            <  1:3643606/100‑1 (MQ=255)

                    cTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCgcgc                                                                                      >  2:60217/1‑89 (MQ=255)

                    cTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAg                                                                           <  1:4726616/100‑1 (MQ=255)

                      ttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCa                                                                                    <  2:3907567/89‑1 (MQ=255)

                       tGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAg                                                                                   <  2:1618911/89‑1 (MQ=255)

                        gCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAga                                                                                  >  2:3539897/1‑89 (MQ=255)

                        gCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAga                                                                                  >  2:2962902/1‑89 (MQ=255)

                        gCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgtg                                                                       <  1:970516/100‑1 (MQ=255)

                            ggATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAgacgac                                                                              >  2:2782485/1‑89 (MQ=255)

                                 gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCg                                                                         >  2:2283268/1‑89 (MQ=255)

                                 gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCg                                                                         >  2:1957315/1‑89 (MQ=255)

                                 gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCg                                                                         >  2:2126772/1‑89 (MQ=255)

                                 gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCg                                                                         >  2:3089295/1‑89 (MQ=255)

                                 gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTg                                                              >  1:4236126/1‑100 (MQ=255)

                                 caaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCg                                                                         >  2:2444084/2‑89 (MQ=255)

                                  aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTgg                                                             >  1:4191699/1‑100 (MQ=255)

                                  aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTgg                                                             <  1:1643885/100‑1 (MQ=255)

                                   aCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgtg                                                                       <  2:1394987/89‑1 (MQ=255)

                                    cGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtg                                                           <  1:1534278/100‑1 (MQ=255)

                                       cgcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGtgct                                                                   <  2:2078686/89‑1 (MQ=255)

                                        gcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGc                                                       <  1:2795578/100‑1 (MQ=255)

                                        gcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGc                                                       >  1:533494/1‑100 (MQ=255)

                                        gcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGc                                                       >  1:2125792/1‑100 (MQ=255)

                                         cAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGcc                                                      <  1:39618/100‑1 (MQ=255)

                                          aGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg                                                     <  1:1247899/100‑1 (MQ=255)

                                           gcgtgcgtGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCgg                                                    <  1:3117886/100‑1 (MQ=255)

                                            cgtgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGt                                                   <  1:234297/100‑1 (MQ=255)

                                             gtgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGtt                                                  >  1:4657671/1‑100 (MQ=255)

                                                    aaCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGcc                                                      <  2:1342269/89‑1 (MQ=255)

                                                     aCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg                                                     >  2:4856793/1‑89 (MQ=255)

                                                      ccGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCgg                                                    >  2:4631332/1‑89 (MQ=255)

                                                      ccGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCgg                                                    >  2:812693/1‑89 (MQ=255)

                                                      ccGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgc                                         >  1:193465/1‑100 (MQ=255)

                                                       cGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGt                                                   <  2:2154974/89‑1 (MQ=255)

                                                         cAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGttt                                                 <  2:287719/89‑1 (MQ=255)

                                                         cAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcg                                      <  1:1080471/100‑1 (MQ=255)

                                                          aGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcgc                                     >  1:843450/1‑100 (MQ=255)

                                                          aGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcgc                                     <  1:1485783/100‑1 (MQ=255)

                                                          aGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcgc                                     <  1:3592632/100‑1 (MQ=255)

                                                           gCTGACTGAACTGAAAAAATAGGCGGTATGCTCTAAAGAGACAATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                                               <  2:17481/89‑1 (MQ=255)

                                                            ctgactgaACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa                                              <  2:365195/89‑1 (MQ=255)

                                                            ctgactgaACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa                                              <  2:1321445/89‑1 (MQ=255)

                                                            ctgactgaACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccg                                   <  1:3714498/100‑1 (MQ=255)

                                                              gactgaACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAgg                                            <  2:474601/89‑1 (MQ=255)

                                                               actgaactgaAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                                               <  2:4052300/85‑1 (MQ=255)

                                                               actgaactgaAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                                               >  1:4052300/1‑85 (MQ=255)

                                                                ctgaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcg                                          >  2:3493970/1‑89 (MQ=255)

                                                                ctgaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcg                                          >  2:1656459/1‑89 (MQ=255)

                                                                  gaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTgg                             >  1:1827905/1‑100 (MQ=255)

                                                                  gaactgaaAAAACATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcg                                        <  2:4485510/89‑1 (MQ=255)

                                                                   aactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgc                                       >  2:1037902/1‑89 (MQ=255)

                                                                   aactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGc                            <  1:1052216/100‑1 (MQ=255)

                                                                    actgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcg                                      <  2:4131972/89‑1 (MQ=255)

                                                                    actgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCa                           <  1:2526942/100‑1 (MQ=255)

                                                                     ctgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaa                          >  1:1157688/1‑100 (MQ=255)

                                                                      tgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaaa                         >  1:47994/1‑100 (MQ=255)

                                                                      tgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaaa                         <  1:2557471/100‑1 (MQ=255)

                                                                       gaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccg                                   >  2:4529334/1‑89 (MQ=255)

                                                                       gaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAt                        >  1:374233/1‑100 (MQ=255)

                                                                         aaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCATCCGACGAGCGTGTGCTGCTTGGGGTTGCCGGTTTTCAGGCGagcgccgcg                                 <  2:4831896/89‑1 (MQ=255)

                                                                         aaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct                      <  1:442876/100‑1 (MQ=255)

                                                                            aaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg                              <  2:443971/89‑1 (MQ=255)

                                                                            aaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCtt                   <  1:1739553/100‑1 (MQ=255)

                                                                             atatGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTgg                             <  2:553494/89‑1 (MQ=255)

                                                                             atatGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTgg                             >  2:3198596/1‑89 (MQ=255)

                                                                              tatGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGc                            <  2:2348165/89‑1 (MQ=255)

                                                                              tatGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGc                            >  2:3275452/1‑89 (MQ=255)

                                                                              tatGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa                 <  1:3324209/100‑1 (MQ=255)

                                                                               atGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCa                           >  2:867198/1‑89 (MQ=255)

                                                                               atGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAg                <  1:2851951/100‑1 (MQ=255)

                                                                                tGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGc               >  1:2703130/1‑100 (MQ=255)

                                                                                 gCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCg              >  1:1129955/1‑100 (MQ=255)

                                                                                    gcaTTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAAc           <  1:354450/98‑1 (MQ=255)

                                                                                      aTTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct                    <  2:75276/89‑1 (MQ=255)

                                                                                        tctcTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCCGGCGCGCGCCGCGCTGGCAAATCTCttt                  <  2:944237/89‑1 (MQ=255)

                                                                                        tctcTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt                  >  2:4411556/1‑89 (MQ=255)

                                                                                         ctctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa                 <  2:1597711/89‑1 (MQ=255)

                                                                                         ctctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCt      >  1:259136/1‑100 (MQ=255)

                                                                                         ctctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCt      >  1:2649932/1‑100 (MQ=255)

                                                                                           ctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGc               >  2:124005/1‑89 (MQ=255)

                                                                                           ctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTc    <  1:3004345/100‑1 (MQ=255)

                                                                                           ctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTc    <  1:3034153/100‑1 (MQ=255)

                                                                                            tAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCg   <  1:3069557/100‑1 (MQ=255)

                                                                                            tAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCg   >  1:3838675/1‑100 (MQ=255)

                                                                                            tAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCg   >  1:4333376/1‑100 (MQ=255)

                                                                                            tAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGATTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCg   >  1:3492732/1‑100 (MQ=255)

                                                                                             aaaGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGa             <  2:3749867/89‑1 (MQ=255)

                                                                                             aaaGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGa             <  2:3471851/89‑1 (MQ=255)

                                                                                             aaaGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCga  <  1:4091718/100‑1 (MQ=255)

                                                                                              aaGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGaa            >  2:3445546/1‑89 (MQ=255)

CTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGA  >  NC_000913/3041535‑3041727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`