| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,387 | C→T | 100% | Q791* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,387 | 0 | C | T | 100.0% | 92.3 / NA | 36 | Q791* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/23); total (13/23) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAG > NC_000913/125252‑125524 | cTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACATCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCCCTTAgtttat > 2:497283/1‑138 (MQ=255) gccaaaGCTTACGGCGTAGGTCCGGACGTTTGGAGGTCGACCTCCTTCAACGACCTGGCGCGTGATGGGCAGGATTGCGACCGCTGGAACAGGCTGCCCCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGaa < 2:338233/140‑1 (MQ=255) cGAAAGATTACGGCGTAGGTTCTGACGTTTCGCGTCGCACTTCTTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAac < 1:366047/143‑1 (MQ=255) gAAAGGTTACGGAGTAGGTTCTGTCGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacg < 1:826846/143‑1 (MQ=255) aTTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGCACGACGCt > 1:332928/1‑143 (MQ=255) ttCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGAGGCTCAGGCAGTGGcgt > 1:13878/1‑141 (MQ=255) ccggatgTTTATAGCGTGATCTACTTGAACGAGTTGGCGTGTGATGGTTAGGCTTGTGATTGATGGATCGTGTATAACCCGCTGGAAACTCCGCGCGTTCAGTCAATCATTTATGTGATGAACGACGCTCCGGCAGTGGCATc < 2:430712/137‑1 (MQ=255) ctggttATATCGTGACCTCCGTCACCGGGCTGGCGCTTGATGGTCAGTATTGTGATCGCTGGAACATGCTGCTCCCGCTGGAAACGCCGCGCGGTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTAcc < 2:215441/139‑1 (MQ=255) aCGTTGATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCCGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTAcc < 1:156343/143‑1 (MQ=255) gTTTATCGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACACGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGa < 1:488607/143‑1 (MQ=255) gTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGa < 1:676459/143‑1 (MQ=255) tataGCGTGACCTCCTTCACCGGGTTGGGTCGCGGTGGTCCGGATTGTGCACGCTGGGACATGCTGCACCCGCTGGAAACGCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACta < 2:869423/143‑1 (MQ=255) taGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACtata < 2:695275/143‑1 (MQ=255) tGACCTACTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGaaa < 1:423912/143‑1 (MQ=255) cACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCACCGGCAGTCGCAGCTACCGACTATATGAAACGGTTCGCTg > 2:384883/1‑143 (MQ=255) cccGCGCGGGCGCGTGATGGCCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGa < 2:724988/142‑1 (MQ=255) ccGAGCTGGCGCGTGAGGGTCAGGATTGTGAACCCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAg < 1:383850/143‑1 (MQ=255) gCTGGCGCGTGATGGGCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAgg < 1:586757/143‑1 (MQ=255) gcgcGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCg > 1:657604/1‑143 (MQ=255) gcgTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCCTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGCCCATATCCAACTGTTCGCTGAGCCGGTCCgga > 2:157980/1‑141 (MQ=255) gcgTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTACGTATATCACTTAGGTGATGAACGACGCTCCGGACCCAACAGCTACCGACTATATGAAACTGTTCGAAGAGCAggacagga > 2:620590/1‑137 (MQ=255) tGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGCTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGGACtt > 2:587460/1‑143 (MQ=255) gTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCATAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGCCTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGtc > 2:94392/1‑142 (MQ=255) tgAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTgacg < 1:467310/143‑1 (MQ=255) tGGGACATGCGGCAGCAGCTGGAATCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACcg < 2:561198/143‑1 (MQ=255) ggAACAGGCTGCACCCGCTGGAAACGCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACcgc < 1:663532/143‑1 (MQ=255) gcaaCATGCCGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACcgc < 1:462084/141‑1 (MQ=255) gAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTCACGTACCGGCTAACGACTaaccag > 2:128605/1‑138 (MQ=255) aCATGCTGCACCCGGTGGAAGCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCAGCTAGCGACTATAGGAAACTCTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTa < 2:530672/143‑1 (MQ=255) ccGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTACGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGa > 1:296571/1‑143 (MQ=255) ccGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGGGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGAACTGGGGACGGa > 1:773914/1‑143 (MQ=255) atgtCGCTTAGGTGACGAACGACGTCCCGGCAGTGGCATCTACCGACTATATGACACTGTTCGCTGAGCAGGTCCGTACTTGCGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGt < 2:27787/140‑1 (MQ=255) atatGGCTTAGGTGACGAACGACGCTCCGGCAGTGGCGTCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGt < 2:246518/143‑1 (MQ=255) atatCCCTTAGGTGATGACCGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGt < 1:300586/143‑1 (MQ=255) atCGCTTAGGTGATGAACGACGCCCCGGCAGTGGCATCTACCGACTATATCAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTga < 1:850110/143‑1 (MQ=255) tCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTAGTCGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACCGCCATgag > 2:744452/1‑143 (MQ=255) | CTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAG > NC_000913/125252‑125524 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |