Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,032,336 G→C 12.7% intergenic (‑19/+48) dcm ← / ← yedJ DNA‑cytosine methyltransferase/putative HD superfamily phosphohydrolase YedJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,032,3360GC12.7% 131.1 / 13.8 54intergenic (‑19/+48)dcm/yedJDNA‑cytosine methyltransferase/putative HD superfamily phosphohydrolase YedJ
Reads supporting (aligned to +/- strand):  ref base G (26/21);  new base C (3/4);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.94e-02

TACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGA  >  NC_000913/2032198‑2032475
                                                                                                                                          |                                                                                                                                           
tACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGAt                                                                                                                                         >  1:416997/1‑143 (MQ=255)
 acacCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGGTTTCACCGGCCATCTGAGATg                                                                                                                                        >  2:442397/1‑143 (MQ=255)
   acCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATTTATTATCCCTCATAGATTTCACCGGCCATCTGAGATGGc                                                                                                                                      <  2:65482/143‑1 (MQ=255)
     cATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTCAGATGGCCg                                                                                                                                    <  1:377467/143‑1 (MQ=255)
      actAAGCTGCGGCACCAACGTGTTAACATCATATATTGGCAGCAGTTTCTCCAGCATGGCCGGTGCGGCGTTCCCGGTGCTGTATGAATCGGTTACTGATATATTTGCCTGCATAGATTTCACCGGCCATCTCAGATGGCCgg                                                                                                                                   <  2:622084/141‑2 (MQ=255)
      aTTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTCAGATGGCCgg                                                                                                                                   <  2:18542/143‑2 (MQ=255)
      aTTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTGTCTGCATAGATTTCACCGGCCATCTGAGATGGCCGa                                                                                                                                   >  1:366472/1‑143 (MQ=255)
          aGCTGCGCCACTAACGTTATAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAgg                                                                                                                               <  2:138211/143‑1 (MQ=255)
               cgcCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGGGATGGCCGACAGGTTAAc                                                                                                                          >  1:687934/1‑143 (MQ=255)
               cgcCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAAc                                                                                                                          >  2:450671/1‑143 (MQ=255)
               cgcCACCAACGTTTTAACATCATAAATTGGCAGCAGATTGGCGAGCGTTGCCTGTGCGGCATTCCCGGGGCTGGATGAGTGGGTGGCGTATATATGGTCCGGCATAGATGTCACCGGCCATGTGAGAGGGCGGACGGGTTagc                                                                                                                          >  2:582470/1‑141 (MQ=255)
                 ccacccacGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACAt                                                                                                                        <  1:630683/143‑1 (MQ=255)
                        cGTTGTACCAGCATACATTTGCAGCAGTTTCTCCAGCATTGCCTGTTCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATCTTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTaac                                                                                                                 <  1:170887/143‑3 (MQ=255)
                          ttttAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTGAACGTGATTACAga                                                                                                               >  1:23042/1‑142 (MQ=255)
                                catcatAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTCTGCATCGGTTCCTGCTATCTTTTGCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATACTTCCAGCCAtttc                                                                                                         >  2:453163/1‑142 (MQ=255)
                                  tcatAACTTGGGCGCAGTTTCTCCAGCATGGCGTGGGCGGTATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGGGATGGCCGACAGGTTAACATAATTACAGCCATTTAgg                                                                                                       <  2:503186/143‑1 (MQ=255)
                                  tcatAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTAATGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGTCGGGTTAGCGTATGTACACCCGTTTggg                                                                                                       >  1:11671/1‑143 (MQ=255)
                                    atAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCCGCATTCCCGTTGCTGTATGAATCGGTTACTGATATAGTTTCCTGCATCGTGTGCACCGCCCATCTGACATGGCCTCCAGGTTAACATAATCACACCCATCTAGGGc                                                                                                     >  2:354701/1‑143 (MQ=255)
                                          gtGCAGCAGTTCCTCCAGCATTGCCTGTGCGGCATTCCCGGCGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCAcgcg                                                                                               <  1:794971/142‑1 (MQ=255)
                                               gcagTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAGTCGGTTACTGATATATCTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTcag                                                                                          <  2:680337/143‑1 (MQ=255)
                                               gcagTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTcag                                                                                          >  2:563012/1‑143 (MQ=255)
                                                  gTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTcagcca                                                                                       <  2:377677/143‑1 (MQ=255)
                                                   tttCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTGACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTcagccag                                                                                      <  1:368704/143‑1 (MQ=255)
                                                       tcCAGCATTGCCTGTGCGGCATTCCCGGTGCAGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCAGACAGGCTAACATAATCACAGCCATATAGGGCCAAGCACACCGGCAGCggc                                                                                  >  2:704010/1‑142 (MQ=255)
                                                       tcCAGCATTGCCGGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATACTTGCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGa                                                                                  <  1:354937/143‑1 (MQ=255)
                                                        ccAGCATTGCCTGTGCGGCATTCCCGGTGCGGTATGAATCGGTTACTGATATATCTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGAt                                                                                 <  2:824086/143‑1 (MQ=255)
                                                           gCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAAACGGCTACTGATATATTTTCCTGCATAGCGTTCACCGGCCATCTGAGATGGCCGACAGGCACACATCATTACAGCCCTGTAGGGCCACGCGCTCCGCCCCCGGaacaa                                                                              >  2:40777/1‑139 (MQ=255)
                                                            cATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATCTTTTCCTGCCTAGATTGCACCGGCCATATGAGATGGCCGACAGGTTAACATAATTACAGCCATGTAGCACCACCACCTCAGCCCGCGGATGcaa                                                                             >  2:843737/1‑143 (MQ=255)
                                                               tGCCTGTGCGGCATTCCCGGTGCTGTATGAAGCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACg                                                                          <  1:753913/143‑1 (MQ=255)
                                                                  cggtgCTGCATTCCCGGTGCTTTAGGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCAt                                                                       <  1:157030/141‑1 (MQ=255)
                                                                  cccgccGGCAGTCCCAGTGCTGTATGAGTCGGGTGCTGATATATTTTCCTGCATAGATTTCCCCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGGCACGCGCTCAGCCAGCGGATGAAAACGCAt                                                                       <  1:115947/138‑1 (MQ=255)
                                                                   tgtgCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAACGAAATAAAACGCATc                                                                      >  1:544210/1‑143 (MQ=255)
                                                                             tCCCGGTGGTGTCTTAATCGGTTTCTGATAGATTTTCCTGCATTGATTTCACCGGCCATCTCAGAAAACTAGCAAGTTAACAAAATTAGAGTCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTtg                                                            <  2:310619/143‑1 (MQ=255)
                                                                                  gtgttttATGAGTCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATGATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATc                                                       <  2:413203/137‑1 (MQ=255)
                                                                                  gTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATc                                                       <  1:93441/143‑1 (MQ=255)
                                                                                      tGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCAGACAGGTTAACATAATTACAGCCACATAGAGCCACACGACCAGAAAGCAGCAGAAAACACCACTATCACCACGTGGCCGaca                                                   >  2:681072/1‑143 (MQ=255)
                                                                                        tATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACAcc                                                 >  1:22081/1‑143 (MQ=255)
                                                                                              tCGGTTACTGATATATTGTCCTGCATAGACTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTCCAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCAtt                                           <  1:588284/143‑1 (MQ=255)
                                                                                                  tcacctatatatCTTCCTGCTTAGATTTCACCGGCCATTTGAGATCGCCGACAGGTTAACAGAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTcc                                       <  2:370225/137‑1 (MQ=255)
                                                                                                      tGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATCCTCCCCCg                                   >  1:728651/1‑143 (MQ=255)
                                                                                                       gATCTATTTTCCTGCATAGCTTTCACCGGCCATCTGTCATGGCCGACAGGTTAACATAATTACAGCCATCTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCGATCACCTTGTGATCGACACCTTCATTCTCCCCCGc                                  <  2:543679/143‑1 (MQ=255)
                                                                                                        atatatTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTCACATAATTACAGCCATCTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCGTGTGAGCGACACCCTCATCCTCCCCCGcc                                 >  2:829785/1‑143 (MQ=255)
                                                                                                        atatatTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTGGTGAGCGACACCTTCATTCTCCCCCGcc                                 >  2:458118/1‑143 (MQ=255)
                                                                                                         tatatTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCCTGTGATCGACACCTTCATTCCCCCCCGCCa                                >  2:388483/1‑143 (MQ=255)
                                                                                                          atatTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATCATTACAGCGATTTAGGGCCACGCGCTCAGCCAGCGGACAAAAACGCATCTATCACCTTGTGATCGACACCCTCAGTCTGCCACCCCAg                               >  2:309954/1‑143 (MQ=255)
                                                                                                              tttCCTGCATAGATTTCCCCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCCGTCCg                           >  1:840449/1‑143 (MQ=255)
                                                                                                                          aacctgtcGGCCATCTCAGATGGCCGGCAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGCCCCCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTc               >  1:598944/8‑143 (MQ=255)
                                                                                                                          aTTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTc               >  1:638875/1‑143 (MQ=255)
                                                                                                                             ctgtcGGCCATCTCAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCGGTTCGGCCCTGACCTTCGcc            >  1:507524/5‑143 (MQ=255)
                                                                                                                              tgtcGGCCATCTCAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCAACTTGCGATCGACACCTTCGTTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCa           >  2:420861/4‑143 (MQ=255)
                                                                                                                                ccGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGCACGAAAACGCATCTATCACCTCGTGATCGACACCCTCATTCTCCCCCGCCAGCTCGGCACTGGTCTTCGCCCTa         >  2:130045/1‑143 (MQ=255)
                                                                                                                                       tCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATCAACTCGa  >  2:610041/1‑143 (MQ=255)
                                                                                                                                       tCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGa  <  1:563012/143‑1 (MQ=255)
                                                                                                                                       tCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGa  <  1:450671/143‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGA  >  NC_000913/2032198‑2032475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑