| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,483,912 | T→A | 11.0% | V53D (GTC→GAC) | evgA → | DNA‑binding transcriptional activator EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,483,912 | 0 | T | A | 11.0% | 86.5 / 3.2 | 37 | V53D (GTC→GAC) | evgA | DNA‑binding transcriptional activator EvgA |
| Reads supporting (aligned to +/- strand): ref base T (18/12); new base A (2/2); total (21/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.93e-01 | |||||||||||
TTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGG > NC_000913/2483771‑2484047 | ttGATGACCATCCTCTTGCTATCGGAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTc < 1:75112/143‑1 (MQ=255)ttGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTc < 1:666197/143‑1 (MQ=255) gaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGatat > 1:413654/1‑143 (MQ=255) ttCCTCTTGCTAGCGCAGTAATTCGTAATTTATTGATCACAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATcccc < 2:712881/142‑1 (MQ=255) gtCCTGTTGCTATCGCAGCAATTCGTAATTTATTGATCAAGAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcagg < 2:499937/142‑4 (MQ=255) tATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACg > 2:511561/1‑143 (MQ=255) ggtCGCAGCCATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACg < 1:69862/141‑1 (MQ=255) tCGCAGCAATTCGTAATTTATTGATTAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGt < 2:218164/143‑1 (MQ=255) agcaATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGAGATCCACGGCGTTAACGGTATcc > 2:225824/1‑143 (MQ=255) gcaATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCa < 1:181336/143‑1 (MQ=255) tcgggaTTTAGTGAGCAAAGAGGATAGTGGAATCTGAGCAGAGGTGAGTGAAGGCGGAAGGGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGAGATCCCCGGAGTTAACGGTATCCAGgtgt < 2:673415/138‑1 (MQ=255) tAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAg > 1:812279/1‑143 (MQ=255) aagtgaTTGATCAACAACGATAGTGAGATCGTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGa < 1:12237/138‑1 (MQ=255) tCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGGATCGAGGTGTTAGAAACGCTGAgg > 1:86145/1‑143 (MQ=255) cAAAAACGATATTCAAATCTTAGCAGAGTTGACTGAAGGCTGAAGTGCCGTTCAGCGCGTGGTGACACTTAAGCCTGCTATCGTCAGCATTGATAGCGATATCACCGGAGGTCACGGTGTCCACGTGTTAGAAAAGCTGCGGa > 2:196819/1‑143 (MQ=255) tatTGAAATCTTAGCAGAGTTGACTGAAGGCGGACGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAat > 1:244809/1‑143 (MQ=255) tGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCactcta > 2:456957/1‑138 (MQ=255) tGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAAtata > 1:748983/1‑143 (MQ=255) gAAATCTTGGCAGGGTGGACTGAAGGCGGAAGGGCCGGTCGGCGGGTGGAAACACTTAAGCGTGATATCGTCATCATTGATGGCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAg < 2:280819/143‑1 (MQ=255) gAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGCTAGAAACGCTGAGGAAGCGCCCATATAg > 2:868555/1‑143 (MQ=255) ttGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATtattattat > 2:786937/1‑143 (MQ=255) tggaGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAACGGAATTGTTATTATCGtac > 2:285940/4‑141 (MQ=255) tGAAGGCGGAAGTGCCGTTCAGCGGGAGGAAACACTTAAGCCTGATAGCGGCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGCGGAAGAGCCAATATAGCGGAATAATTAGTATCGtct > 1:860777/1‑143 (MQ=255) ggaGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGtctc < 1:470806/141‑1 (MQ=255) gTGCCGTGCAGCGGGTGGAAACCCTTAAGCCTTATGTCCGCATCATTGATGTCGATATCCTCGGGGTTAACTGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAAt < 2:54723/143‑1 (MQ=255) tGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTAGATATACCCGGAGTTAACGGTATCAAGGTGTTAGAAACGCTGAGGAAGCCCGAATATAGAGAAATTATGATTATCGTCTCCGCTcaaaggt > 2:342890/1‑140 (MQ=255) gcagTTCAGCGGGTGGAAGCACGTAAGCCGGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGa < 1:729269/140‑1 (MQ=255) gCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCAttttt > 1:556527/1‑143 (MQ=255) gTGGAAACACTTAAGCCTGATATCGTCATGATTGATGTCGATATCCCAGGAGTTAACGGTATCCAGTTGTTCGACACGCTGAGGCCGCGCCACTAAACCCGAACCATACTTCTCCTCTCACCTCCCAACGAACATTTTTACgg > 2:557583/1‑143 (MQ=255) gggAGACCCTTCAGCCTGATATCGACATCATTGAGGGCGATCACCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCGGAGGCAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACggg < 1:264202/142‑1 (MQ=255) aaaCACTTAAGCCTGATATCGTCATCATTGATGTCGATCTCCCCGGAGTTAACGGTATCCCGGTGTTAGACACTCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCATCTCCGCTAAAAATGACCATTTTTACGGGaaa > 1:329965/1‑143 (MQ=255) aaaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACTCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGaaa < 1:532295/143‑1 (MQ=255) aaaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCACGGAGTTAACGGTATCCAGGTGTTAGAAGCGCTGAGGAAGCGCCAATATAGCCGAATTATTATTGTCGTCTCCGCTAAAAAAGACCATTTTTACGGCaaa > 2:752111/1‑143 (MQ=255) tatCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAGACATTGTGCTGATGCTg > 1:516911/1‑143 (MQ=255) atCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTgg > 1:432147/1‑143 (MQ=255) cGTCATCATTGATGACGATATCCCCGGAGTTAACGGTATAAGGGTGTTATAGTCACTGAGGAAGCGCCAATATATCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGgcg < 1:834934/143‑1 (MQ=255) atTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAAAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATAGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATgg > 1:311938/1‑143 (MQ=255) | TTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGG > NC_000913/2483771‑2484047 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |