Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,661,969 C→G 100% V55L (GTT→CTT)  iscR ← DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,661,9690CG100.0% 165.1 / ‑6.7 55V55L (GTT→CTT) iscRDNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base G (29/24);  minor base T (0/1);  total (29/26)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAG  >  NC_000913/2661829‑2662108
                                                                                                                                            |                                                                                                                                           
gccgccCTGGCAGCCGCCTTTCCCCTGCCAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCa                                                                                                                                           <  2:319435/143‑1 (MQ=255)
      cTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACGTCGCCAACGGCGATGCTGCTGGCAGCTTTGCCTAACAGGTAACCACCGCCTGGGCCAAGTACGCTGGCACGCAGACCag                                                                                                                                     >  2:172477/1‑142 (MQ=255)
      cTGGCAGCCGCCTTTACCCTGACAAAGCATGCCCTCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTCCGCTGGAACGCAGACCag                                                                                                                                     >  2:722030/1‑142 (MQ=255)
       tGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACAAtt                                                                                                                                    >  1:97545/1‑143 (MQ=255)
             ccgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACg                                                                                                                              <  2:750564/143‑1 (MQ=255)
              cgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTGACGc                                                                                                                             <  1:205941/143‑1 (MQ=255)
                    tACCCTGACACCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACgg                                                                                                                       <  2:558739/143‑1 (MQ=255)
                      cccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGa                                                                                                                     <  1:656938/143‑1 (MQ=255)
                       ccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGCAAGCAGACCATTTTTACGCAGACGGGag                                                                                                                    >  1:215442/1‑142 (MQ=255)
                       ccTGACAACGGGTGGCATCTACAGATTCGTCAAAAGCGACAATTATTTGGCGTGCGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTAGGCTGGAAAGCAGACCATTTTTACGCCGACGGGaa                                                                                                                    >  1:691460/1‑143 (MQ=255)
                             aaCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAgt                                                                                                              <  1:879802/143‑1 (MQ=255)
                                      cATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATGTTTACGCAGACGGGAAAACAGTTGTCCCAGa                                                                                                     >  1:777429/1‑143 (MQ=255)
                                       aTCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGACTAACAGATAACGCCCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGGTCCAGAt                                                                                                    >  2:184682/1‑143 (MQ=255)
                                         cTACAGATTCGTCAGCGGCGCTAATTACTCCGCCACCGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCCCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATaa                                                                                                  <  1:225417/143‑1 (MQ=255)
                                           aCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGTAGCTGCTGCCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGa                                                                                                <  2:854980/143‑1 (MQ=255)
                                            cAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTACCAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTCTACGCAGACGGGAAAACAGTTGTTCCAGATAAGaa                                                                                               >  2:682416/1‑143 (MQ=255)
                                             ggagtCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCCCCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGaaa                                                                                              <  1:252/139‑1 (MQ=255)
                                               aTTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGCCCCAGATAAgcacgg                                                                                            >  2:610199/1‑138 (MQ=255)
                                                   gTCCACCGCGCTAATTACTCCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGaaa                                                                                        <  2:730745/143‑1 (MQ=255)
                                                        ccgcgcTAATTACTCCTCCAACCGCGATGCTGCTTGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACTTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAATAGTTGTTCCAGATAAGAAAGGGAAATTccc                                                                                   <  1:648805/141‑1 (MQ=255)
                                                        cGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTccc                                                                                   <  1:565975/143‑1 (MQ=255)
                                                          gcgcTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTg                                                                                 <  1:434643/143‑1 (MQ=255)
                                                              tAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAACGGGAAATTCCCTGACGt                                                                             >  1:246406/1‑143 (MQ=255)
                                                              tAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGt                                                                             >  2:590937/1‑143 (MQ=255)
                                                              tAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTAAGCAGACGGGAAAACAGTTATTAAAGATAAGAAAGGGAAATTCCCTGACGt                                                                             >  1:276196/1‑143 (MQ=255)
                                                                   aCTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGCCAGACAGCTGTTAACGACTAGGAAGGGGGATTCCCCGAccgccgga                                                                        >  2:615465/1‑136 (MQ=255)
                                                                     ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa                                                                      >  2:272293/1‑143 (MQ=255)
                                                                     tgcGCCAGCGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa                                                                      <  1:241763/141‑1 (MQ=255)
                                                                       cGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGCAAACCGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAata                                                                    >  2:549895/1‑143 (MQ=255)
                                                                        gCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat                                                                   <  1:134017/143‑1 (MQ=255)
                                                                        gCCAACGGCGATGCTGCCGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAATCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat                                                                   <  1:187558/143‑1 (MQ=255)
                                                                                    gctgctGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTATACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTGCGGAAATATTACCCAACGGTa                                                       >  2:706353/1‑143 (MQ=255)
                                                                                      tgctgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGCAAATATCCGCCAACCGGAcc                                                     >  1:279642/1‑143 (MQ=255)
                                                                                      tgctgGCATCTTTACCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATCTCAGCCAACGGTAcc                                                     >  1:508021/1‑143 (MQ=255)
                                                                                       gctgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCg                                                    <  1:689435/143‑1 (MQ=255)
                                                                                         tgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCggg                                                  >  1:732811/1‑143 (MQ=255)
                                                                                                    ccgaaCAGATAACCACCGCCGGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCaga                                       <  2:800367/140‑1 (MQ=255)
                                                                                                    ccTAACAGATAACCACCGCCTGCTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGACATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCaga                                       <  2:84087/143‑1 (MQ=255)
                                                                                                      tAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGt                                     >  2:499968/1‑143 (MQ=255)
                                                                                                         cAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGa                                  <  1:499968/143‑1 (MQ=255)
                                                                                                          aGATAACCACCGCCTGGTCAACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAg                                 <  1:107051/143‑1 (MQ=255)
                                                                                                           tatgcccaccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgc                                <  2:753453/138‑1 (MQ=255)
                                                                                                            gcttccaccTCCTGGGTCAGGTACGCGGGAAACCAGACCATTTTTACGGAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgcg                               <  1:869874/139‑1 (MQ=255)
                                                                                                              aaCCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAAGTCCCTGACGTTCGGAAATATCAGCCAACGGGACCGGGCCCGCGTCAGAGTGGAGCGCa                             >  1:733053/1‑143 (MQ=255)
                                                                                                                  accGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGCGTTGAGCGCAACGt                         >  2:585400/1‑143 (MQ=255)
                                                                                                                     gCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCCGCCAACGGTACCGGGCCCGCTTCGGAGTTTAGCGCAACGTCaa                      >  1:5214/1‑143 (MQ=255)
                                                                                                                           tCCACGTACGCTGGAAAGCAGACCATTTTTACGCCGCCCGGAGGACAGTAGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGAGCAACGTCCAGCATTg                >  1:167667/1‑143 (MQ=255)
                                                                                                                             cACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCg              <  1:554906/143‑1 (MQ=255)
                                                                                                                                gTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGACCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTc           >  1:831845/1‑143 (MQ=255)
                                                                                                                                  aCGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCAc         <  1:272293/143‑1 (MQ=255)
                                                                                                                                     cTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGc      >  1:101948/1‑143 (MQ=255)
                                                                                                                                      tGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGACATATCAGCCAACGGTACCGGGCCCGCTCCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCa     >  2:12933/1‑143 (MQ=255)
                                                                                                                                      tGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCa     >  1:606908/1‑143 (MQ=255)
                                                                                                                                      tGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCa     >  1:316870/1‑143 (MQ=255)
                                                                                                                                         aaaGCAGCCCACTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGTGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAg  <  1:690423/143‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
GCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAG  >  NC_000913/2661829‑2662108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑