Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,041,632	T→A	100%	V107E (GTG→GAG)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,632	0	T	A	100.0%	170.1 / NA	57	V107E (GTG→GAG)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (25/32);  total (25/32)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGG  >  NC_000913/3041492‑3041771                                                                                                                                             |                                                                                                                                            cgccCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTCAAgaga                                                                                                                                           >  2:168270/1‑143 (MQ=255)  gccCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGAc                                                                                                                                          <  1:523801/143‑1 (MQ=255)       ttGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACCGAACTGAAAAAATATGCGGTATTCTCTCAAGAGACCATcg                                                                                                                                     >  2:559429/1‑143 (MQ=255)           gACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAACAATATGCGGTATTCTCTAAAGAGACCATCGCGcc                                                                                                                                 >  1:594760/1‑143 (MQ=255)           gACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGcc                                                                                                                                 >  2:396923/1‑143 (MQ=255)              gCCACAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAga                                                                                                                              <  1:488245/143‑1 (MQ=255)                 aaaGGTAAACTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGAGGGTTTTTCAGGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAgacga                                                                                                                           <  2:574569/143‑1 (MQ=255)                 aaaGGTAAAATGTGGAGCAAGGTACGTGTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCGCTAAAGAGACCATCGCGCCAgacga                                                                                                                           <  2:589360/143‑1 (MQ=255)                   aGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGacgacg                                                                                                                         >  2:11509/1‑143 (MQ=255)                        aagtgtgGAGCAATTGACGTCTGTTCCGCGGCGGCGATGGCTTTGCATGGAGTGACCGGCGCAGCGTGCGTGAGCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgt                                                                                                                    <  2:245627/140‑1 (MQ=255)                                     tttACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCgcgc                                                                                                                                  <  2:670870/116‑1 (MQ=255)                                     tttACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCgcgc                                                                                                                                  >  1:670870/1‑116 (MQ=255)                                     tttACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtg                                                                                                       <  2:744117/143‑1 (MQ=255)                                       tACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTAAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGAGTGCTGCTGGGTGtt                                                                                                     >  2:81190/1‑143 (MQ=255)                                          gTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGAGTGCGTGAATCGAAGTTGACTGCAATAATTAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCGGACGACGAGCGTGTGCTGCTTGGTGTTGcc                                                                                                  >  1:407810/1‑143 (MQ=255)                                             ggaaccGCGACGGCGGGGGCTAAGGCAGGAGTGAACGGCGCAGCGTGCGTGATCCGCAGAAAAAAAAAAAAAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGt                                                                                               <  2:126476/139‑1 (MQ=255)                                               ttCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGttt                                                                                             >  2:117787/1‑143 (MQ=255)                                               ttCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGGTCCCGGatt                                                                                             >  2:444071/1‑143 (MQ=255)                                               ttCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGAGTGTGCTGCTTGGGGTTGCCGGttt                                                                                             >  1:708133/1‑143 (MQ=255)                                                tCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGtttt                                                                                            <  1:720959/143‑1 (MQ=255)                                                 ccGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTc                                                                                           >  1:336395/1‑143 (MQ=255)                                                  cgcgACGGCGATGGCTTTGCAGGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa                                                                                          <  2:470423/143‑1 (MQ=255)                                                   gcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAg                                                                                         <  1:680114/143‑1 (MQ=255)                                                           gATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACAGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcgc                                                                                 >  2:720187/1‑143 (MQ=255)                                                                  ttGGATGGACTGACCGGCGCAGCGTGCGTGACCCGCAGATTACTTAACTGTAAAAATATGCGTTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg                                                                          <  2:127517/143‑1 (MQ=255)                                                                    gCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGc                                                                        <  1:704201/143‑1 (MQ=255)                                                                      aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaa                                                                      >  1:687912/1‑143 (MQ=255)                                                                       tGGATTGAACGGCGCAGCGGGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaaa                                                                     <  2:494885/143‑1 (MQ=255)                                                                          gttGAACGGCGGCGCGTGCGTGAACCGCAGCGGACTGAACTGAGAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct                                                                  <  2:76961/142‑1 (MQ=255)                                                                              aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt                                                              <  1:11509/143‑1 (MQ=255)                                                                                 ggCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGc                                                           >  2:556501/1‑143 (MQ=255)                                                                                    gcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGaa                                                        <  2:108853/143‑1 (MQ=255)                                                                                    gcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGaa                                                        <  1:292624/143‑1 (MQ=255)                                                                                    gcAGCGTGCGGGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCTTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGaa                                                        <  2:579167/143‑1 (MQ=255)                                                                                         gtgcgggAACCGCTGCGTACTGAACTTGAAGAATATGCGGTATTCTCGAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTCGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGcc                                                   <  2:155063/143‑1 (MQ=255)                                                                                          tgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCt                                                  >  1:802287/1‑143 (MQ=255)                                                                                           gcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCtt                                                 <  1:181149/143‑1 (MQ=255)                                                                                           gcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCtt                                                 >  1:762757/1‑143 (MQ=255)                                                                                              tgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGGGCGCCGCGCTCGCGAGTCGGCTTAGCGAACTGCCGTCgc                                              >  2:1731/1‑142 (MQ=255)                                                                                              tgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCga                                              <  2:851243/143‑1 (MQ=255)                                                                                              tgAACCCCTGCTAACTGAACTGATAACGTAAGCGCATTGGTCTGTAGAGACCCTCGCGCCAGACGACGAGCGTGTGCTGCTTCGTGTTGCCGGTTTTCAGCCGCGCGCCGCGCTGGCAAATCTCTTTAGCTAACTGCCTTCga                                              <  2:619910/143‑1 (MQ=255)                                                                                                aaCCGCAGCTGGCTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa                                            <  2:512274/143‑1 (MQ=255)                                                                                                  ccGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaaga                                          <  1:260634/143‑1 (MQ=255)                                                                                                  ccGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaaga                                          >  1:295785/1‑143 (MQ=255)                                                                                                       gCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCCAAGCACCTTAACGCTTTCTTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAAc                                     <  1:200327/143‑1 (MQ=255)                                                                                                         tgactgaACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAAGCGTGCGCCGAGCTGGCAAATCGCTTTGGCGAACTGCCTACGAAAGagaagcgg                                   >  2:171794/1‑139 (MQ=255)                                                                                                         taactgaactgaAGAAATATGCGGTATTCTCTAAAGAGACCAGCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAg                                   <  2:455087/141‑1 (MQ=255)                                                                                                           actgaactgaAAAGAGATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACAACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGt                                 <  1:711909/143‑1 (MQ=255)                                                                                                             tgaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAg                               >  1:577253/1‑143 (MQ=255)                                                                                                                     aaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGACCGAACAACCGGTAGTCCaagaa                       >  1:585006/1‑143 (MQ=255)                                                                                                                      aaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAACGAAAAACAGGTAGTCAaagaag                      >  1:566491/1‑143 (MQ=255)                                                                                                                          catGCGGTATTCTCTAAAGAGACCATCGCGCCAGCCGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgcgc                  <  1:251302/142‑1 (MQ=255)                                                                                                                           atGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGcgcg                 <  1:520143/143‑1 (MQ=255)                                                                                                                              cGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGAcc              >  1:371376/1‑143 (MQ=255)                                                                                                                                  aTTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTGTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGCAAGAGACACAGGTAGTCAAAGGAGGCGCGACCACtt          >  1:428105/1‑143 (MQ=255)                                                                                                                                       ctAAAGAGACCATCGCGCCAGGCGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTa     <  1:396923/143‑1 (MQ=255)                                                                                                                                       ctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTa     <  1:720187/143‑1 (MQ=255)                                                                                                                                          aaGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAGGAAAAACAGGTGGTCAAAGAAGGCGCGACCACTTTGGTATgg  >  1:484994/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                            CGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGG  >  NC_000913/3041492‑3041771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑